DNA replicating cells were detected in the psoriatic epidermis by anti BrdU antibody after incubating tissue sections biopsied in acute and chronic areas with BrdU. The expression pattern of No. 1 keratin, involucrin. filaggrin, loricrin and transglutaminase E was studied in the paraffin embedded sections of psoriatic specimen which showed various histopathological findings. The results are as follows 1) The labeling index of BrdU in lesional psoriatic skin was significantly greater than that in normal skin. The labeling index was greater in the clinically active lesion of psoriasis than that of the chronic lesion, which suggest that psoriatic lesions are composed of distinct lesions differing in activity. 2) In the epidermis of the psoriatic plaques with extensive parakeratosis or microabscesses at the base of the stratum corneum and absent granular layers, the expression pattern of five epidermal proteins appeared as follows : No. 1 keratin which is found in normal epidermis immediately above the basal layer, appeared several layers higher. Involucrin was detected in most of the suprabasal layers. Fillaggrin, loricrin and transglutaminase E showed negative expression. The results obtained in the present study suggest that the increment of DNA replicating cells and aberrant, maturation pathway of epidermis appear in active psoriatic plaques.
Bromodeoxyuridine ; DNA ; Epidermis ; Paraffin ; Parakeratosis ; Psoriasis ; Skin*

No abstract available.
Bromodeoxyuridine* ; Cervix Uteri* ; Drug Therapy* ; Female

No abstract available.
Bromodeoxyuridine* ; Hep G2 Cells* ; Kinetics* ; Tretinoin*

PURPOSE: We investigated the inhibitory effects of mitomycin-C (MMC) and 5-fluorouracil (5-FU) on the proliferation of the lens epithelial cells (LECs) depending on time and concentration during the epithelial cell culture using capsular bag model. METHODS: We cultured LECs using capsular bag model after exposure to various time and concentration of MMC and 5-FU. We compared the half coverage time and appearance of posterior capsule by LECs. We examined the proliferative and inhibitory effect on LECs using BrdU immune staining. We observed the morphologic change of LECs on histologic section. RESULTS: Half coverage time of posterior capsule by LEC was 18.2 +/- 3.5 days in control group, whereas 27.5 +/- 3.8 days and 26.8 +/- 4.2 days when treated with 0.2 mg/ml MMC, 50 mg/ml 5-FU for 3 minutes, repectively. The increase of concentration and the exposure time of MMC or 5-FU resulted in the delay of coverage time of posterior capsule by LECs and reduction of BrdU incorporation in the nucleus of proliferating cells. On histologic section, reduction of LECs' multilayering and few cytoplasmic organells were observed. CONCLUSIONS: Using capsular bag model, we found the inhibitory effect of MMC and 5-FU on LECs proliferation depending on the concentration and exposure time. Capsular bag model would be contribute to the study about after-cataract.
Bromodeoxyuridine ; Cytoplasm ; Epithelial Cells* ; Fluorouracil* ; Mitomycin*

BACKGROUND: Anesthetics have been suspected of impairing various aspects of the immune function either directly by affecting the function of immunocompetent cells or indirectly by modulating the stress response. Splenocytes play important roles in the cellular host defense against infection. In order to assess the immune modulatory effects of propofol, this study examined the cytotoxic and proliferative effects of propofol on splenocytes. METHODS: Splenocytes, as responders, were isolated from BALB/c mice (n = 10). The cells were pretreated with different propofol concentrations (0micrometer, 30micrometer, 100micrometer, 300micrometer) for 24 hours. The cytotoxic effect was assayed by the NADH dehydrogenase activity and the proliferation was evaluated by the level of 5-bromo-2'-deoxyunridine (BrdU) incorporation during DNA synthesis in the presence or absence of propofol, in addition to lipopolysaccharide (LPS, 1 microgram/ml) for mitogenic stimulation. A cell proliferation enzyme-linked immuno-sorbent assay (ELISA) system was used, and the stimulation index was calculated in the presence or absence of propofol. RESULTS: The percentage of the NADH dehydrogenase activity was changed by the propofol pretreatment (P < 0.001). LPS stimulation significantly decreased the NADH dehydrogenase activity at 100micrometer and 300micrometer compared with the propofol-added or pretreated cells (P < 0.05). The stimulation index to LPS was lower at concentrations of 100micrometer and 300micrometer than at 30micrometer, and proliferative response of splenocytes were completely abrogated by adding toxic concentrations (100micrometer) of propofol (P < 0.05). CONCLUSIONS: Neither cytotoxicity, as defined by the NADH dehydrogenase activity, nor a proliferative effect, as measured by the level of (BrdU) incorporation in the splenocytes, were affected by the clinical concentration of propofol.
Anesthetics ; Animals ; Bromodeoxyuridine ; Cell Proliferation ; DNA ; Mice ; NADH Dehydrogenase ; Propofol

BACKGROUND: Various aspects of immunological homeostasis are affected by anesthesia and surgery, including the function of immunocompetent cells and the modulation of stress responses. To evaluate immunologic changes that occurred following propofol and enflurane anesthesia, we evaluated the proliferative responsiveness of peripheral blood mononuclear cells (PBMC) in patients undergoing laparoscopic gynecologic surgery. METHODS: PBMC were isolated from patients prior to anesthesia and on the first postoperative day (n = 10). The proliferative response was then evaluated based on the level of 5-bromo-2-deoxyunridine (BrdU) incorporation that occurred during DNA synthesisafter the induction of mitogenic stimulation by treatment with 1 microgram/ml lipopolysaccharides (LPS). To accomplish this, cell proliferation was assayed by enzyme-linked immuno-sorbent assay (ELISA), after which a stimulation index was calculated. RESULTS: Although the calculated stimulation index decreased in response to both propofol and enflurane anesthesia, the stimulation index did not differ significantly between groups. However, following stimulation with LPS, the stimulation index was significantly higher in the enflurane group than in the propofol group (P < 0.05). CONCLUSIONS: Propofol and enflurane anesthesia inhibit the PBMC proliferation. However, the decrease in proliferation that occurred in response to enflurane was attenuated by LPS.
Anesthesia ; Bromodeoxyuridine ; Cell Proliferation ; DNA ; Enflurane ; Homeostasis ; Humans ; Lipopolysaccharides ; Propofol

PURPOSE: To determine whether the delivery of the SV40 large T-antigen is a feasible method for transiently inducing proliferation of corneal endothelial cells, we delivered liposome-protein complex into bovine corneal endothelial cells(BCEC). METHOD: SV40 large T-antigen protein was introduced into BCEC and positive cells were identified by immunohistochemistry. Quiescent BCECs were double-labeled using BrdU as a measure of de novo DNA synthesis and the Ki-67 was detected by standard immunohistochemical methods. RESULT: The treatment of quiescent BCECs with large T antigen caused an increase in BrdU incorporation and Ki-67 expression. It was tested by time-course study. CONCLUSION: This finding suggests that liposome-mediated delivery of transforming proteins could be a method to transiently induce corneal endothelial cell proliferation.
Antigens, Viral, Tumor* ; Bromodeoxyuridine ; Cell Proliferation ; DNA ; Endothelial Cells* ; Immunohistochemistry

PURPOSE: To investigate the effect of low dose radiation on neuronal cell proliferation in diabetic rats. MATERIALS AND METHODS: A group of rats (first group) were divided into three subgroups (nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy groups) to determine the effect of radiation on normal hippocampal neuronal cell proliferation. A further group of rats (second group) were divided into six subgroups (nondiabetic control, diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy groups) to determine the effect of radiation on hippocampal neuronal cell proliferation under diabetic conditions. Using immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU), the number of neuronal cells in the dentate gyrus of all the groups was counted. RESULTS: The number of BrdU-positive cells in the dentate Gyrus of the nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy subgroups of the first group were 45.96+/-3.42, 59.34+/-5.20 and 19.26+/-2.98/mm2, respectively. The number of BrdU-positive cells in the dentate gyrus of the diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy subgroups of the second group were 55.44+/-8.57, 33.33+/-6.46, 67.75+/-10.54, 66.63+/-10.05, 23.59+/-6.37 and 14.34+/-7.22/mm2, respectively. CONCLUSION: Low dose radiation enhances cell proliferation in the dentate gyrus of STZ-induced diabetic rats.
Animals ; Bromodeoxyuridine ; Cell Proliferation* ; Dentate Gyrus ; Hippocampus ; Immunohistochemistry ; Neurons* ; Rats*

PURPOSE: To investigate the effect of low dose radiation on neuronal cell proliferation in diabetic rats. MATERIALS AND METHODS: A group of rats (first group) were divided into three subgroups (nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy groups) to determine the effect of radiation on normal hippocampal neuronal cell proliferation. A further group of rats (second group) were divided into six subgroups (nondiabetic control, diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy groups) to determine the effect of radiation on hippocampal neuronal cell proliferation under diabetic conditions. Using immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU), the number of neuronal cells in the dentate gyrus of all the groups was counted. RESULTS: The number of BrdU-positive cells in the dentate Gyrus of the nondiabetic control, nondiabetic 0.1 Gy and nondiabetic 10 Gy subgroups of the first group were 45.96+/-3.42, 59.34+/-5.20 and 19.26+/-2.98/mm2, respectively. The number of BrdU-positive cells in the dentate gyrus of the diabetic control, diabetic 0.01 Gy, diabetic 0.1 Gy, diabetic 1 Gy and diabetic 10 Gy subgroups of the second group were 55.44+/-8.57, 33.33+/-6.46, 67.75+/-10.54, 66.63+/-10.05, 23.59+/-6.37 and 14.34+/-7.22/mm2, respectively. CONCLUSION: Low dose radiation enhances cell proliferation in the dentate gyrus of STZ-induced diabetic rats.
Animals ; Bromodeoxyuridine ; Cell Proliferation* ; Dentate Gyrus ; Hippocampus ; Immunohistochemistry ; Neurons* ; Rats*

Physeal distraction is used for limb lengthening or correction of deformities in skeletally immature patients. But the effect of distraction on the physis is uncertain. The young rabbits were arranged into five groups according to the slow distraction rates: Group I (no distraction), Group II (distraction rate of 0.25mm per day), Group III (distraction rate of 0.5mm per day), Group IV (distraction rate of 0.75 mm per day), Group V (distraction rate of 1mm per day). The tibial length, size of proliferating zone, microscopic findings of physis immunostained with bromodeoxyuridine, and physeal response to physeal distraction on each group were studied. The results were as follows. 1. There was an increase in bone length on the distracted side (1.8 to 4.2mm). 2. There was an irregular increase in the thickness of the distracted physis. 3. There was an abnormal accumulation of hypertrophic chondrocytes in hypertrophic zone in distracted physis. 4. There was no evidence of anomalous cell proliferation, in the resting, proliferating and hypertrophic zones. These results conclude that the physeal distraction does not stimulate cell proliferation in the physis, even when it is seen to be thickened after the chondrodiatasis.
Bromodeoxyuridine ; Cell Proliferation ; Chondrocytes ; Congenital Abnormalities ; Extremities ; Humans ; Rabbits*