1.Relationship Between Cytogenetic Complexity and Peritumoral Edema in High-Grade Astrocytoma.
Kyung Ho JEONG ; Young Jin SONG ; Jin Yeong HAN ; Ki Uk KIM
Annals of Laboratory Medicine 2016;36(6):583-589
BACKGROUND: The purpose of the study is to reveal the association of cytogenetic compltyexi and peritumoral edema volume (PTEV) and its prognostic significance in high-grade astrocytoma patients by culturing patient tumor cells. METHODS: Twenty-seven high-grade astrocytoma patients were divided into three groups according to karyotype complexity: normal, non-complex karyotype (NCK), and complex karyotype (CK). Endothelial growth factor receptor (EGFR) amplification was detected by FISH, and its association with chromosome 7 abnormalities was analyzed. Mean PTEV of each group was compared by ANOVA to evaluate the relationship between PTEV and cytogenetic complexity. RESULTS: The PTEV of patients in normal (n=6), NCK (n=8), and CK (n=13) groups were 24.52±17.73, 34.26±35.04, and 86.31±48.7 cm3, respectively (P=0.005). Ten out of 11 patients with EGFR amplification showed abnormalities in chromosome 7. The mean PTEV of EGFR-amplified and non-amplified groups were 80.4±53.7 and 41.3±37.9 cm3, respectively (P=0.035). The average survival of patients with PTEV less than 90 cm3 was 30.52±26.11 months, while in patients with PTEVs over or equal to 90 cm3, it was 10.83±5.53 months (P=0.007). CONCLUSIONS: The results show an association of complex karyotype with the PTEV of high-grade astrocytoma. EGFR amplification plays a significant role in the formation of peritumoral edema, causing PTEV to increase, which is related with survival. This implies that cytogenetic karyotype can be applied as a prognostic factor.
Adult
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Aged
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Astrocytoma/diagnostic imaging/mortality/*pathology
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Brain Neoplasms/diagnostic imaging/mortality/*pathology
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Chromosome Aberrations
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Chromosomes, Human, Pair 7
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Edema/diagnostic imaging/pathology
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Female
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Humans
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In Situ Hybridization, Fluorescence
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Kaplan-Meier Estimate
;
Karyotype
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Magnetic Resonance Imaging
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Male
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Middle Aged
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Neoplasm Grading
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Prognosis
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Receptors, Vascular Endothelial Growth Factor/metabolism
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Tumor Cells, Cultured
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Young Adult