3.Mechanisms of lysosomal proteases participating in cerebral ischemia-induced neuronal death.
Ai-Ping QIN ; Hui-Ling ZHANG ; Zheng-Hong QIN
Neuroscience Bulletin 2008;24(2):117-123
There are three different types of cell death, including apoptosis (Type I), autophagic cell death (Type II), and necrosis (Type III). Ischemic neuronal death influences stroke development and progression. Lysosomes are important organelles having an acidic milieu to maintain cellular metabolism by degrading unneeded extra- and intracellular substances. Lysosomal enzymes, including cathepsins and some lipid hydrolases, when secreted following rupture of the lysosomal membrane, can be very harmful to their environment, which results in pathological destruction of cellular structures. Since lysosomes contain catalytic enzymes for degrading proteins, carbohydrates and lipids, it seems natural that they should participate in cellular death and dismantling. In this review, we discuss the recent developments in ischemic neuronal death, and present the possible molecular mechanisms that the lysosomal enzymes participate in the three different types of cell death in ischemic brain damage. Moreover, the research related to the selective cathepsin inhibitors may provide a novel therapeutic target for treating stroke and promoting recovery.
Animals
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Apoptosis
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Autophagy
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Brain Infarction
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enzymology
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physiopathology
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Brain Ischemia
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enzymology
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physiopathology
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Cathepsins
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metabolism
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Humans
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Lysosomes
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metabolism
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Necrosis
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physiopathology
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Nerve Degeneration
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enzymology
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physiopathology
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Peptide Hydrolases
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metabolism
4.Interferon-stimulated gene 15 as a general marker for acute and chronic neuronal injuries.
Ren-Gang WANG ; Marcus KAUL ; Dong-Xian ZHANG
Acta Physiologica Sinica 2012;64(5):577-583
Activation of interferon (IFN) signaling in the central nervous system (CNS) is usually associated with inflammation. However, a robust activation of type I IFN-stimulated genes (ISGs) at pre-symptomatic stages occurs in the spinal cord of SOD1(G93A) mice, an amyotrophic lateral sclerosis (ALS) animal model, without obvious signs of inflammation. To determine if the same signaling pathway is elevated in other types of neuronal injuries, we examined the protein expression levels of an IFN-stimulated gene, ISG15, in mouse models of acute and chronic neuronal injuries. We found that ISG15 protein was dramatically increased in the brains of mice subjected to global ischemia and traumatic brain injury, and in transgenic mice overexpressing HIV gp120 protein. These results suggest that activation of ISGs is a shared feature of neuronal injuries and that ISG15 may be a suitable biomarker for detecting neuronal injuries in the CNS.
Amyotrophic Lateral Sclerosis
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physiopathology
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Animals
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Brain Injuries
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physiopathology
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Brain Ischemia
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physiopathology
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Central Nervous System
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physiopathology
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Cytokines
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metabolism
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Disease Models, Animal
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Mice
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Mice, Transgenic
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Ubiquitins
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metabolism
5.Molecular Biological Roles of Oxidative Stress in Acute Brain Ischemia.
Xiang-yi KONG ; Jian GUAN ; Ren-zhi WANG
Acta Academiae Medicinae Sinicae 2016;38(2):222-227
The pathogenesis of acute brain ischemia is very complex, involving multiple mechanisms including excessive free radical generation. Oxidative stress means the imbalance between the generation and removal of free radicals. Once acute brain ischemia occurs, the reactive oxygen species interact with large numbers of biomacromolecules, irreversibly change or destroy the functions of cellular lipids, proteins, and nucleic acids, and thus initiate cell signaling pathways. However, the molecular biological characteristics of oxidative stress and the way to prevent and treat acute brain ischemia still need further investigations.
Brain Ischemia
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metabolism
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physiopathology
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Humans
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Oxidative Stress
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Reactive Oxygen Species
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metabolism
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Signal Transduction
8.Effect of progesterone on MMP-3 expression in neonatal rat brain after hypoxic-ischemia.
Chun-Yang XU ; Shuang LI ; Xin-Qiang LI ; Dong-Liang LI
Chinese Journal of Applied Physiology 2010;26(3):370-373
OBJECTIVETo study the effect of progesterone on matrix metalloproteinase-3 (MMP-3) expression in neonatal rat brain after hypoxic-ischemia.
METHODSFollowed the hypoxic-ischemia of neonatal rat brain, Evans blue (EB) staining and transmission electron microscopy were used to detect the blood-brain barrier pathological changes on permeability. MMP-3 protein expression in cerebral cortex was measured with Western blot.
RESULTSTransmission electron microscopy results showed that the blood brain barrier in hypoxic-ischemic group changed significantly compare to progesterone group. EB staining results suggested that the blood-brain barrier permeability of hypoxic-ischemic group was significantly increased compared to sham-operated group (P < 0.01). The blood-brain barrier permeability in progesterone group was also decreased in comparison to that of hypoxic-ischemic group (P < 0.05). Western blot image analysis results indicated that MMP-3 protein expression in the hypoxic-ischemic group increased significantly than that in sham-operated group (P < 0.01), and the progesterone group was decreased significantly than that in hypoxic-ischemic group (P < 0.05).
CONCLUSIONProgesterone may reduce the blood-brain barrier damage by reducing MMP-3 expression. This might be one of the protective mechanisms in the hypoxic-ischemic brain injury.
Animals ; Animals, Newborn ; Blood-Brain Barrier ; physiopathology ; Hypoxia-Ischemia, Brain ; metabolism ; pathology ; physiopathology ; Matrix Metalloproteinase 3 ; metabolism ; Progesterone ; pharmacology ; Rats ; Rats, Sprague-Dawley
9.The expression of bFGF, GAP-43 and neurogenesis after cerebral ischemia/reperfusion in rats.
Wang-Qing SHI ; Guan-Yi ZHENG ; Xiao-Dong CHEN ; Yuan-Gui ZHU ; Jing ZHANG ; Qiong JIANG
Chinese Journal of Applied Physiology 2013;29(1):63-67
OBJECTIVETo observe time points of the expressions of basic fibroblast growth factor (bFGF), growth associated protein-43 (GAP-43) and neurogenesis after cerebral ischemia/reperfusion in rats and explore its possible mechanism of neurogenesis.
METHODSModels of middle cerebral artery occlusion (MCAO) were established in SD rats which were divided into 3 d, 7 d, 14 d and 28 d groups (n = 6). The neurological severity was evaluated by neurological severity scores (NSS) and scores of motor test (SMT). Neuronal injury in the boundary zone of the infarction area was evaluated by TUNEL and Nissl staining; The expressions of bFGF and GAP-43 and neurogenesis were evaluated by Western blot and 5-bromodeoxyuridine (Brdu) fluorescence staining, respectively.
RESULTSIt showed up neurologic impairment and motor dysfunction after cerebral ischemia/reperfusion in rats at 3 d, the numbers of neuron apoptosis also peaked at 3d, the protein levels of bFGF and GAP-43 were significantly increased in time-dependent manner, peaked at 7 d and then decreased gradually, meanwhile, Brdu and NeuN double fluorescence staining displayed scattered Brdu-and NeuN-positive cells in the boundary zone of the infarction area.
CONCLUSIONThese results suggest that the upregulation of bFGF and GAP-43 may contribute to the neurogenesis after cerebral ischemia/reperfusion.
Animals ; Brain Ischemia ; metabolism ; physiopathology ; Fibroblast Growth Factor 2 ; metabolism ; GAP-43 Protein ; metabolism ; Male ; Neurogenesis ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; metabolism ; physiopathology
10.Progress in the studies on neuronal nitric oxide synthase inhibitors.
Chao-Zai ZHANG ; Lei DONG ; Feng-Hui MU ; Xiao-Hong YANG ; Wei SUN
Acta Pharmaceutica Sinica 2014;49(6):781-788
Nitric oxide (NO), which is involved in the regulation of the cardiovascular system, nervous system, immune system, reproductive system, digestive system and other physiological activities, is an important biological substance with activity. Under normal physiological conditions, neuronal nitric oxide synthase (nNOS) can precisely regulate the nervous system NO production, release, diffusion and inactivation processes. But an excess of NO associates with the development of cerebral ischemia, Alzheimer's and Parkinson's psychosis nervous system diseases, while inhibition of nNOS activity can regulate the content of NO in vivo, and produce a therapeutic effect on some of the nervous system diseases. This review mainly describes the structure and regulation of nNOS and recent developments of small molecule inhibitors of nNOS.
Alzheimer Disease
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physiopathology
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Brain Ischemia
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physiopathology
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Humans
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Nitric Oxide
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metabolism
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Nitric Oxide Synthase Type I
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antagonists & inhibitors
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metabolism
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Parkinson Disease
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physiopathology