HUANG Yuan-yu's understanding of viscera qi and blood based on the theory of rise and fall of middle qi. He believes that rise and fall of middle qi plays a vital role in the normal operation of the function of organs and blood, so the blood stasis and qi disorder of spleen and stomach can cause the dysfunction of other organs. Therefore, he adopts the method of rising and falling middle qi and four dimensions, which can provide new ideas for clinical treatment of qi and blood stasis syndrome.

442 cases of primary liver carcinoma were admitted to our hospital from August 1975 to June 1983, and confirmed by surgery and pathologic examination. Of these 442 cases, 71 cases (16.8%) were AFP-negative (h magglutination titer 1:10, counter immuno-electrophoresis negative and radio-immuno-assay less than 31ng/ml). The ege of the patients ranged from 15 to 76 years old(average : 46), and the sex ratio between males and females was 4.9 = 1.The pathologic findings were 59 liver cell carcinomas, 10 bile duct cell carcinomas, 2 mixed types and 34(57.6%)pertaining to grade I or I-II, 22(37.3%) grade II-III or III and 3(5.1 %)grade III-IV in differentiation. 31 cases (43.6%) were found to have been combined witn cirrhosis.The most common clinical symptom was hepatodynia and other symptoms were anorexia, s:nse of abdomen distention,fever etc. 10 of the cases were misdiagnosed as caver- nous hemangicma of the liver, diseases of bile ducts and hydatid cyst of the liver before operation.Earlier diagnosis could be made by combining enzyme tcsts(PHI, LDH, rGT, AKP, A1AT etc),B-mode ultrasonography, angiograpby of hepatic artery, liver scanning and clinical symptons together.In addition, an evaluation of enzyme tests, B-mode ultrasonography,liver scanning and angiography of hepatic artery were also discussed as well as the differential diagnosis.

Objective:To study the history of migraineurs,and find out the historical features which have higher value in migraine diagnosis and differential diagnosis.Method:250 patients who have primary headache were chosen in this study(include 117 migraineurs),each patient,s history was taked in detail,then each historical feature,s diagnostic value were analysised using statistic method.Results:In all the historical features that we studied pain exacerbated by activity,pain throbbing,disability,phonophobia, nausea,photophobia,pain on oneside,all have high sensitivity and high specificity.Conclusion:Known the sensitivity and specificity of each historical features,can help us to diagnose migraine rapidly and accurately.

Objective To study the longitudinal change of Migraine patient′s condition ,quality of life and disability ;analyze the evolution of the relationship between headache frequency ,pain intensity ,medication and treatment efficacy .Methods Continuous registration of migraine patients ,using WHO-DAS Ⅱ to assess the disability ,using MSQ2 .1 to assess the health-related quality of life ,using headache diary to record the patient′s condition .Results Compared the conditions of before and after treatment ,the aver-age level of pain ,acute drug using and headache frequency ,were significantly improved .The three dimensions′scores of MSQ2 .1 were decreased .Conclusion In this study ,significant improvement were found in some of the disability and health-related quality of life .Continuity of treatment has an important role to improve health-related quality of life of patients and reduce disability .

The da Vinci Surgical System has developed and great progress has been made recently in surgery. Despite lack of applications in tumors of head and neck, its advantages, such as minimally invasive procedure and rapid recovery, show great prospects. In this article, we reviewed the surgical method, indication, advantages and prospects of its application in tumors of head and neck.

ObjectiveTo evaluate the effects of aerobic exercise, nutrition or both on physical fitness and metabolism in obesity adolescents. Methods94 overweight/obese adolescents (12～16 years old) underwent 10 weeks exercise alone, nutrition intervention alone, and exercise combined with nutrition, respectively. The physical and metabolism index were detected before and after intervention. ResultsThe body mass index (BMI), body fat, body fat% decreased in all the groups. Especially, exercise alone decreased total cholesterol (TC), increased fasting insulin (FINS), insulin resistance (HOMA-IR), and function of β-cells (HOMA-β). Nutrition alone decreased TC, low-density lipoprotein cholesterol (LDL-C). Exercise combined with nutrition decreased TC and LDL-C, increased FINS and HOMA-β. High-density lipoprotein cholesterol (HDL-C) tended to decrease in all the groups. ConclusionThe aerobic exercise and /or nutrition intervention can improve the lipid metabolism, Exercise can improve function of the β-cell of pancreas. But the effects of exercise and/or nutrition on HDL-C and insulin resistance are not satisfactory.

Objective:To investigate the ability of fluoride toothpaste and fluoride varnish on enamel remineralization and acid resist-ance.Methods:Enamel specimens were prepared from bovine incisors,and were randomly divided into 4 groups(n=3)after acid-etching by 37%phosphoric acid.Specimens in group A(control)was processed daily with normal saline;those in group B and C were treated once with Duraphat varnish and Fluor Protector varnish respectively;in group D was daily processed with fluoride toothpaste. All specimens were incubated in artificial saliva for 2 weeks.Then all specimens received acid-etching again.Micro-hardness test, SEM observation and image analysis were performed before and after each step.Results:After 2 weeks of processing,no remineraliza-tion was found in group A.Varnish layers were observed on the surface of specimens in group B and C.In group D remineralization was detected on the enamel surface.After re-etching,micro-hardness decreased in group A and D.Fluoride varnish layers in group B and C showed strong resistance to acid-etching.After re-etching,area of micro-holes in group A and D increased(P<0.05 ),but that in group D was smaller than in the control(P<0.05).No micro-hole was observed in group B and group C.Conclusion:Protec-tive layer formed on the enamel surface by fluoride varnish is resistant to acid-etching and promotes enamel remineralization.Fluoride toothpaste application can promote enamel remineralization,but with less resistance to acid.

Apigenin is a plant flavonoid which has various biological activities and pharmacological effects including anti-tumor, antioxidant, anti-inflammatory, sedative and so on, particularly in the anti-tumor areas. It can inhibit proliferation, invasion, metastasis and angiogenesis of tumor cells. In addition, it can also induce apoptosis and enhance chemotherapy sensitivity.

Objective To develop Z-score reference ranges for tricuspid annular plane systolic excursion(TAPSE) in normal fetuses from the measurements of gestational age(GA),biparietal diameter (BPD) or femur length(FL) using fetal echocardiography.Methods A retrospective cross sectional study of 1012 singleton normal fetuses were performed.The gestation age ranged from 20 to 41 weeks.Non-cardiac fetal biometric parameters included GA and BPD and FL were measured and calculated GA based on menstrual age.TAPSE was measured in a standard apical four-chamber view by free angle M-mode echocardiography.Normal Z-score ranges were developed for TAPSE using GA,BPD and FL as independent variables.These were accomplished by using first standard regression analysis and then weighted regression of absolute residual values for each parameter in order to adjust for inconstant variance.Results Linear regression model was the best description of the data in each case and correlations between TASPE and independent variables (GA,BPD,FL) were excellent.Heteroscedasticity of standard deviation (SD) with increasing independent variables also could be modeled with a simple linear regression.According to these equations,TAPSE Z-score =(the actual measurement of TAPSE-predicted TAPSE)/prediction SD.Conclusions Normal reference ranges and Z-scores for TAPSE have been provided.These normative data may be useful tools for assessment of fetal TAPSE,to evaluate fetal right ventricular function more accurately and effectively.

Cancer immunotherapy is a new approach to cancer treatment. Dendritic cells, the most potent antigen-presenting cells found in humans, have attracted a lot of researches. However, dendritic cells are insufficient to cross-present self-antigens in-vivo. To improve the dendritic cells' antigen-presenting capabilities and the anti-tumor effect, in vitro preparation of dendritic cell vaccine and combination with pro-apoptotic treatments such as chemotherapy are being used.