1.Antibodies to borrelia burgdorferi in subjects with high risk of exposure to ticks.
Journal of the Korean Society for Microbiology 1992;27(3):277-282
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Ticks*
2.Indirect immunofluorescent antibody titers of apodemus agrarius serum against borrelia burgdorferi.
Hyung Hoan LEE ; Hyun KANG ; yunsop CHONG ; Hun Gil LIM
Journal of the Korean Society for Microbiology 1992;27(2):163-171
No abstract available.
Animals
;
Borrelia burgdorferi*
;
Borrelia*
;
Murinae*
3.Serological and antigenic analysis against borrelia burgdorferi of febrile patients in Korea.
Kyung Hee PARK ; Seung Hyun LEE ; Won Jong JANG ; Sang Mahn KIM ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1993;28(5):397-408
No abstract available.
Borrelia burgdorferi*
;
Borrelia*
;
Humans
;
Korea*
4.Immunoblotting analysis of antibodies against borrelia burgdorferi, the lyme disease agent, in sera from the Korean residents.
Sang Nae CHO ; Tae Yoon LEE ; Mi Kyeong LEE ; Dook Soon KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(3):263-272
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Immunoblotting*
;
Lyme Disease*
5.Production and characterization of monoclonal antibodies to borrelia burgdorferi, the lyme disease agent.
Jeon Soo SHIN ; Sang Nae CHO ; Jung Lim LEE ; Sun PARK ; Kyeong Han YOON ; Jae Myun LEE ; Mi Kyeong LEE ; Se Jong KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1992;27(6):537-544
No abstract available.
Antibodies, Monoclonal*
;
Borrelia burgdorferi*
;
Borrelia*
;
Lyme Disease*
6.Retrospective survey of antibody response against borrelia burgdorferi in febrile pateints in 1990.
Sun Ho KEE ; Kyu Jam HWANG ; Hee Bok OH ; Kyung Seok PARK
Journal of the Korean Society for Microbiology 1993;28(6):462-471
No abstract available.
Antibody Formation*
;
Borrelia burgdorferi*
;
Borrelia*
;
Retrospective Studies*
7.Isolation of borrelia burgdorferi, the causative agent of lyme disease, from ixodes ticks in Korea.
Kyung Hee PARK ; Seung Hyun LEE ; Woong Jae WON ; Won Jong JANG ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1992;27(4):307-312
No abstract available.
Borrelia burgdorferi*
;
Borrelia*
;
Ixodes*
;
Korea*
;
Lyme Disease*
;
Ticks*
8.Classification of Borrelia burgdorferi Sensu Lato Isolated in Korea by Restriction Fragment Length Polymorphism.
Kyung Hee PARK ; Myong Joon HAHN ; Seung Hyun LEE ; Jong Hyun KIM
Journal of the Korean Society for Microbiology 1998;33(2):119-128
The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (SS) genes are duplicated in tandem. Twelve Korean Borrelia isolates were classified by the method of rRNA gene restriction fragment length polymorphism (RFLP) and Southern hybridization. One of the isolates, KW-3, as well as the representative of ribotype group IV Ip89 appeared to have evolved within B. garinii. The size of the restriction bands of the 11 isolates showed different patterns from the USA and European isolates. The result by RFLP of rrf-rrl intergenic spacer region amplified by PCR showed a good correlation with that of Southern hybridization of rRNA gene. Therefore, the eleven Korean isolates could be classified as members of a new Borrelia group.
Borrelia burgdorferi Group*
;
Borrelia burgdorferi*
;
Borrelia*
;
Classification*
;
Genes, rRNA
;
Korea*
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length*
;
Ribotyping
9.First delection of Borrelia burgdorferi sensu stricto genotype from Hyalomma asiaticum in Karamay, Xinjiang Uygur Autonomous Region of China.
Jingyun DU ; Andong WANG ; Lumeng MU ; Ke ZHANG ; Yuanzhi WANG ; Jun XU ; Chuangfu CHEN ; Lina WANG ; Jinliang SHENG ; Weize ZUO
Chinese Journal of Epidemiology 2015;36(5):539-540
10.Establishment of multiple locus variable-number tandem repeat analysis assay for genotyping of Borrelia burgdorferi sensu lato detected in China.
Xin ZHOU ; ; Xue Xia HOU ; Zhen GENG ; Rui ZHAO ; Kang Lin WAN ; Qin HAO ;
Biomedical and Environmental Sciences 2014;27(9):665-675
OBJECTIVEHuman Lyme Borreliosis (LB), which is caused by Borrelia burgdorferi sensu lato (B. burgdorferi), has been identified as a major arthropod-borne infectious disease in China. We aimed to develop a multiple locus variable-number tandem repeat (VNTR) analysis (MLVA) assay for the genotyping of Borrelia burgdorferi strains detected in China.
METHODSB. garinii PBi complete 904.246 kb chromosome and two plasmids (cp26 and lp54) were screened by using Tandem Repeats Finder program for getting potential VNTR loci, the potential VNTR loci were analyzed and identified with PCR and the VNTR loci data were analyzed and MLVA clustering tree were constrcted by using the categorical coefficient and the unweighted pair-group method with arithmetic means (UPGMA).
RESULTSWe identified 5 new VNTR loci through analyzing 47 potential VNTR loci. We used the MLVA protocol to analyse 101 B. burgdorferi strains detected in China and finally identified 51 unique genotypes in 4 major clusters including B. burgdorferi sensu stricto (B.b.s.s), B. garinii, B. afzelii, and B. valaisiana, consistent with the current MLSA phylogeny studies. The allele numbers of VNTR-1, VNTR-2, VNTR-3, VNTR-4, and VNTR-5 were 7, 3, 9, 7, and 6. The Hunter-Gaston index (HGI) of five VNTR loci were 0.79, 0.22, 0.77, 0.71, and 0.67, respectively. The combined HGI of five VNTR loci was 0.96. Clustering of the strains of Xinjiang, Inner Mongolia and Heilongjiang was confirmed, and this situation was consistent with the close geographical distribution of those provinces.
CONCLUSIONThe MLVA protocol esytablished in this study is easy and can show strains' phylogenetic relationships to distinguish the strains of Borrelia species. It is useful for further phylogenetic and epidemiological analyses of Borrelia strains.
Borrelia burgdorferi Group ; genetics ; China ; Genotyping Techniques ; Minisatellite Repeats