1.Indirect immunofluorescent antibody titers of apodemus agrarius serum against borrelia burgdorferi.
Hyung Hoan LEE ; Hyun KANG ; yunsop CHONG ; Hun Gil LIM
Journal of the Korean Society for Microbiology 1992;27(2):163-171
No abstract available.
Animals
;
Borrelia burgdorferi*
;
Borrelia*
;
Murinae*
2.Serological and antigenic analysis against borrelia burgdorferi of febrile patients in Korea.
Kyung Hee PARK ; Seung Hyun LEE ; Won Jong JANG ; Sang Mahn KIM ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1993;28(5):397-408
No abstract available.
Borrelia burgdorferi*
;
Borrelia*
;
Humans
;
Korea*
3.Antibodies to borrelia burgdorferi in subjects with high risk of exposure to ticks.
Journal of the Korean Society for Microbiology 1992;27(3):277-282
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Ticks*
4.Production and characterization of monoclonal antibodies to borrelia burgdorferi, the lyme disease agent.
Jeon Soo SHIN ; Sang Nae CHO ; Jung Lim LEE ; Sun PARK ; Kyeong Han YOON ; Jae Myun LEE ; Mi Kyeong LEE ; Se Jong KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1992;27(6):537-544
No abstract available.
Antibodies, Monoclonal*
;
Borrelia burgdorferi*
;
Borrelia*
;
Lyme Disease*
5.Retrospective survey of antibody response against borrelia burgdorferi in febrile pateints in 1990.
Sun Ho KEE ; Kyu Jam HWANG ; Hee Bok OH ; Kyung Seok PARK
Journal of the Korean Society for Microbiology 1993;28(6):462-471
No abstract available.
Antibody Formation*
;
Borrelia burgdorferi*
;
Borrelia*
;
Retrospective Studies*
6.Immunoblotting analysis of antibodies against borrelia burgdorferi, the lyme disease agent, in sera from the Korean residents.
Sang Nae CHO ; Tae Yoon LEE ; Mi Kyeong LEE ; Dook Soon KIM ; Joo Deuk KIM
Journal of the Korean Society for Microbiology 1991;26(3):263-272
No abstract available.
Antibodies*
;
Borrelia burgdorferi*
;
Borrelia*
;
Immunoblotting*
;
Lyme Disease*
7.Isolation of borrelia burgdorferi, the causative agent of lyme disease, from ixodes ticks in Korea.
Kyung Hee PARK ; Seung Hyun LEE ; Woong Jae WON ; Won Jong JANG ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1992;27(4):307-312
No abstract available.
Borrelia burgdorferi*
;
Borrelia*
;
Ixodes*
;
Korea*
;
Lyme Disease*
;
Ticks*
8.Classification of Borrelia burgdorferi Sensu Lato Isolated in Korea by Restriction Fragment Length Polymorphism.
Kyung Hee PARK ; Myong Joon HAHN ; Seung Hyun LEE ; Jong Hyun KIM
Journal of the Korean Society for Microbiology 1998;33(2):119-128
The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (SS) genes are duplicated in tandem. Twelve Korean Borrelia isolates were classified by the method of rRNA gene restriction fragment length polymorphism (RFLP) and Southern hybridization. One of the isolates, KW-3, as well as the representative of ribotype group IV Ip89 appeared to have evolved within B. garinii. The size of the restriction bands of the 11 isolates showed different patterns from the USA and European isolates. The result by RFLP of rrf-rrl intergenic spacer region amplified by PCR showed a good correlation with that of Southern hybridization of rRNA gene. Therefore, the eleven Korean isolates could be classified as members of a new Borrelia group.
Borrelia burgdorferi Group*
;
Borrelia burgdorferi*
;
Borrelia*
;
Classification*
;
Genes, rRNA
;
Korea*
;
Polymerase Chain Reaction
;
Polymorphism, Restriction Fragment Length*
;
Ribotyping
9.groEL Gene Analysis of Borrelia afzelii Isolated in Korea.
Hyo Soon PARK ; Jung Hee LEE ; Eun Ju JEONG ; Won Jong JANG ; Kyung Hee PARK ; Bum Joon KIM ; Yoon Hoh KOOK ; Seung Hyun LEE
Journal of Bacteriology and Virology 2004;34(1):1-8
Eleven Borrelia afzelii strains, isolated from Ixodes nipponensis and Apodemus agrarius in Korea, were characterized by groEL gene analysis. Results from previous studies suggested that the groEL gene, which encodes the 60-kDa heat shock protein GroEL, was useful for the differentiation of B. burgdorferi sensu lato. The B. afzelii isolates could be divided into two groups by the phylogenetic tree constructed by UPGMA method and Tsp509 I PCR-RFLP analysis. The result suggested that the groEL gene is useful for identification and characterization of B. burgdorferi sensu lato though a short DNA fragment (310 bp) of the gene was sequenced and compared each other, and that Korean B. afzelii strains are heterogeneous genotypically.
Animals
;
Borrelia burgdorferi Group*
;
Borrelia*
;
DNA
;
Heat-Shock Proteins
;
Ixodes
;
Korea*
;
Murinae
;
Population Characteristics
10.groEL Gene Analysis of Borrelia afzelii Isolated in Korea.
Hyo Soon PARK ; Jung Hee LEE ; Eun Ju JEONG ; Won Jong JANG ; Kyung Hee PARK ; Bum Joon KIM ; Yoon Hoh KOOK ; Seung Hyun LEE
Journal of Bacteriology and Virology 2004;34(1):1-8
Eleven Borrelia afzelii strains, isolated from Ixodes nipponensis and Apodemus agrarius in Korea, were characterized by groEL gene analysis. Results from previous studies suggested that the groEL gene, which encodes the 60-kDa heat shock protein GroEL, was useful for the differentiation of B. burgdorferi sensu lato. The B. afzelii isolates could be divided into two groups by the phylogenetic tree constructed by UPGMA method and Tsp509 I PCR-RFLP analysis. The result suggested that the groEL gene is useful for identification and characterization of B. burgdorferi sensu lato though a short DNA fragment (310 bp) of the gene was sequenced and compared each other, and that Korean B. afzelii strains are heterogeneous genotypically.
Animals
;
Borrelia burgdorferi Group*
;
Borrelia*
;
DNA
;
Heat-Shock Proteins
;
Ixodes
;
Korea*
;
Murinae
;
Population Characteristics