1.Histopathological changes of testes and eyes by neutron irradiation with boron compounds in mice.
Yeon Joo KIM ; Won Ki YOON ; SI Yun RYU ; Ki Jung CHUN ; Hwa Young SON ; Sung Whan CHO
Journal of Veterinary Science 2006;7(1):19-23
This study was performed to investigate the biological effects of boron neutron capture therapy (BNCT) on the testes and eyes in mice using HANARO Nuclear Reactor, Korea Atomic Energy Research Institute. BNCT relies on the high capacity of (10)B in capturing thermal neutrons. Sodium borocaptate (BSH, 75 ppm, iv) and boronophenylalanine (BPA, 750 ppm, ip) have been used as the boron delivery agents. Mice were irradiated with neutron (flux: 1.036739E +09, Fluence 9.600200E+12) by lying flat pose for 30 (10 Gy) or 100 min (33 Gy) with or without boron carrier treatment. In 45 days of irradiation, histopathological changes of the testes and eyes were examined. Thirty-three Gy neutron irradiation for 100 min induced testicular atrophy in which some of seminiferous tubules showed complete depletion of spermatogenic germ cells. Lens epithelial cells and lens fiber were swollen and showed granular changes in an exposure time dependent manner. However, boron carrier treatment had no significant effect on the lesions. These results suggest that the examination of histopathological changes of lens and testis can be used as "biological dosimeters" for gauging radiation responses and the HANARO Nuclear Reactor has sufficient capacities for the BNCT.
Animals
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Boranes/*pharmacology
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Borohydrides/*pharmacology
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Boron Neutron Capture Therapy/*methods
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Eye/pathology/*radiation effects
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Histocytochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Neutrons
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Phenylalanine/*analogs&derivatives/pharmacology
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Seminiferous Tubules/pathology/*radiation effects
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Specific Pathogen-Free Organisms
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Sulfhydryl Compounds/*pharmacology
2.Push-out bond strengths of fiber-reinforced composite posts with various resin cements according to the root level.
Hoon Sang CHANG ; Young Sin NOH ; Yoon LEE ; Kyung San MIN ; Ji Myung BAE
The Journal of Advanced Prosthodontics 2013;5(3):278-286
PURPOSE: The aim of this study was to determine whether the push-out bond strengths between the radicular dentin and fiber reinforced-composite (FRC) posts with various resin cements decreased or not, according to the coronal, middle or apical level of the root. MATERIALS AND METHODS: FRC posts were cemented with one of five resin cement groups (RelyX Unicem: Uni, Contax with activator & LuxaCore-Dual: LuA, Contax & LuxaCore-Dual: Lu, Panavia F 2.0: PA, Super-Bond C&B: SB) into extracted human mandibular premolars. The roots were sliced into discs at the coronal, middle and apical levels. Push-out bond strength tests were performed with a universal testing machine at a crosshead speed of 0.5 mm/min, and the failure aspect was analyzed. RESULTS: There were no significant differences (P>.05) in the bond strengths of the different resin cements at the coronal level, but there were significant differences in the bond strengths at the middle and apical levels (P<.05). Only the Uni and LuA cements did not show any significant decrease in their bond strengths at all the root levels (P>.05); all other groups had a significant decrease in bond strength at the middle or apical level (P<.05). The failure aspect was dominantly cohesive at the coronal level of all resin cements (P<.05), whereas it was dominantly adhesive at the apical level. CONCLUSION: All resin cement groups showed decreases in bond strengths at the middle or apical level except LuA and Uni.
Adhesives
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Bicuspid
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Boron Compounds
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Collodion
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Composite Resins
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Dental Cements
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Dentin
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Humans
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Methacrylates
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Methylmethacrylates
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Resin Cements
3.The effect of surface treatment conditioning on shear bond strength between zirconia and dental resin cements.
Ji Hye KIM ; Jae Min SEO ; Seung Geun AHN ; Ju Mi PARK ; Kwang Yeob SONG
The Journal of Korean Academy of Prosthodontics 2013;51(2):73-81
PURPOSE: The purpose of this study was to evaluate the effect of surface treatment on the shear bond strength of zirconia ceramic to 3 resin cements. MATERIALS AND METHODS: A total of 143 disk-shaped Zirconia blocks (HASS Co., Gangneung, Korea) were randomly divided into three treatment groups: (1) only 50 microm Al2O3 sandblasting, (2) 50 microm Al2O3 sandblast and zircona liner, (3) 50 microm Al2O3 sandblasting and Rocatec (3M ESPE, Seefeld, Germany). Bistite II (Tokuyama Dental Co., Japan), Panavia F (Kuraray Medical, Japan), and Superbond C&B (Sun Medical, Japan) were used to cement onto the zirconia. After 24h of storage in distilled water, shear bond strength was evaluated. High value group was re-tested after thermocycling at 5,000 cycles(5-55degreesC). Shear bond strength data were analyzed with one-way ANOVA, two-way ANOVA test and Post Hoc Test (alpha=.05). Shear bond strength data before and after thermocycling were analyzed with Independent sample T test (alpha=.05). RESULTS: Super-bond C&B treated with Rocatec showed the most high shear bond strength. Super-bond C&B groups resulted in significantly higher than other cement groups (P<.05). Rocatec groups resulted in significantly higher than other surface treatment groups (P<.05). Shear bond strength has increased in Panavia F treated with Zirconia liner (P<.05). After thermocycling, shear bond strength was increased in Super-bond C&B treated with Rocatec but decreased in other groups (P<.05). CONCLUSION: Super-bond C&B cement resulted the highest shear bond strength and Rocatec system enhanced the shear bond strength. After thermocycling, shear bond strength has decreased in most resin cements except Super-bond C&B treated with Rocatec.
Boron Compounds
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Ceramics
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Dental Cements
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Methacrylates
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Methylmethacrylates
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Resin Cements
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Resins, Synthetic
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Water
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Zirconium
4.Detection and monitoring of enamel early caries in vivo using red fluorescence analysis by quantitative light-induced fluorescence method.
Xiao LIU ; Xiao-ping LUO ; Ning WANG ; Li ZHU
West China Journal of Stomatology 2010;28(3):273-277
OBJECTIVETo study the effects of two primers on the bond strengths between casting pure titanium and resin cements.
METHODSTwo sizes of specimen were fabricated by casting, which diameter were 4 mm and 5 mm respectively, and then were cut into 3 mm thick titanium discs. The titanium surfaces were polished with silicon carbide sand papers under running water and then treated by sandblasting. The two size of titanium discs were ultrasonic cleaned and treated with Alloy Primer, V-Primer or without primer, and then bonded in pairs with the resin-based cements Super-Bond C&B, Panavia F and Rely X Unicem. The specimens were then stored in distilled water (37 degrees C) for 24 h. Bond strengths were determined before and after 5 000 thermocycling. The titanium surface morphous and failure mode of the teat specimen were observed.
RESULTSThe shear bond strengths of titanium treated by Alloy Primer to Super-Bond C&B were (31.23 +/- 4.86), (34.08 +/- 3.36) MPa before and after 5 000 thermocycling, which higher than that of other combinations of primers and resin cements.
CONCLUSIONBond strengths were influenced by primers, resin cements, and their combinations. The highest bond strengths were the combinations of Alloy Primer and Super-Bond C&B.
Boron Compounds ; Dental Bonding ; Dental Enamel ; Fluorescence ; Methacrylates ; Methylmethacrylates ; Resin Cements ; Thiones ; Titanium ; Triazines
5.Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell.
Kyung Jin CHOI ; Kab Sung KIM ; Se Hoon KIM ; Dong Kwan KIM ; Hyung Seo PARK
The Korean Journal of Physiology and Pharmacology 2010;14(2):105-111
Inositol 1,4,5-trisphosphate receptors (InsP3Rs) modulate Ca2+ release from intracellular Ca2+ store and are extensively expressed in the membrane of endoplasmic/sarcoplasmic reticulum and Golgi. Although caffeine and 2-aminoethoxydiphenyl borate (2-APB) have been widely used to block InsP3Rs, the use of these is limited due to their multiple actions. In the present study, we examined and compared the ability of caffeine and 2-APB as a blocker of Ca2+ release from intracellular Ca2+ stores and Ca2+ entry through store-operated Ca2+ (SOC) channel in the mouse pancreatic acinar cell. Caffeine did not block the Ca2+ entry, but significantly inhibited carbamylcholine (CCh)-induced Ca2+ release. In contrast, 2-APB did not block CCh-induced Ca2+ release, but remarkably blocked SOC-mediated Ca2+ entry at lower concentrations. In permeabilized acinar cell, caffeine had an inhibitory effect on InsP3-induced Ca2+ release, but 2-APB at lower concentration, which effectively blocked Ca2+ entry, had no inhibitory action. At higher concentrations, 2-APB has multiple paradoxical effects including inhibition of InsP3-induced Ca2+ release and direct stimulation of Ca2+ release. Based on the results, we concluded that caffeine is useful as an inhibitor of InsP3R, and 2-APB at lower concentration is considered a blocker of Ca2+ entry through SOC channels in the pancreatic acinar cell.
Acinar Cells
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Animals
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Boron Compounds
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Caffeine
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Calcium
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Carbachol
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Inositol 1,4,5-Trisphosphate Receptors
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Membranes
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Mice
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Reticulum
6.Apical microleakage of MTA with 4-META/MMA & TBB resin as a root-end filling material.
Jin Cheol KIM ; Mi Ri KIM ; Hyun Jung KO ; Won Kyung YANG
Journal of Korean Academy of Conservative Dentistry 2009;34(4):371-376
We evaluated in vitro microleakage of Mineral Trioxide Aggregate (MTA) powder with 4-methacryloxyethyl trimellitate anhydride (4-META) / methyl methacrylate (MMA) & tri-n-butylborane (TBB) resin as a retrograde filling material by using methylene blue dye method. Fifty-two single rooted, extracted teeth were instrumented and obturated with gutta percha and AH plus sealer. The apical 3mm of each root was resected and 3mm deep ultrasonic root end preparation was done. External surface of roots was coated with nail varnish. Prepared teeth were randomly divided into five groups; Negative control: completely covered with nail varnish; Positive control: coated with nail varnish except for apical foramen; Group 1 (retrofilled with Portland cement); Group 2 (retrofilled with MTA); Group 3 (retrofilled with MTA powder mixed with 4-META/MMA & TBB resin). Immediately after completion of root-end filling, all specimens were submerged in methylene blue dye for 72 hours in 37degrees C incubator. The roots were longitudinally sectioned and measured for extent of dye penetration by three different examiners under microscope (x10). The results were statistically analyzed using one way ANOVA and Turkey's HSD test. No leakage was evident in negative control and complete leakage in positive control group. Group 3 showed significantly less leakage than group 1 and 2 (p < 0.01). There was no significant difference between group 1 and 2 (p > 0.01). It was concluded that MTA powder with 4-META/MMA & TBB resin was excellent in reducing initial apical microleakage.
Aluminum Compounds
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Boron Compounds
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Calcium Compounds
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Drug Combinations
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Epoxy Resins
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Glutamates
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Guanine
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Gutta-Percha
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Incubators
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Methylene Blue
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Nails
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Oxides
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Paint
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Root Canal Filling Materials
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Silicates
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Tooth
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Ultrasonics
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Pemetrexed
7.A comparative study on the dislodging force of magnetic attachment to the denture resin by magnetic design and fixing materials.
Jung Hwa LEE ; Jong Hyuk LEE ; In Ho CHO
The Journal of Korean Academy of Prosthodontics 2008;46(3):261-268
STATEMENT OF PROBLEM: Detachment of the magnetic assembly from the denture base has been a problem in magnetic overdenture patients. PURPOSE: The objectives of this study were to compare the dislodging force by the fixing materials and the designs of the magnetic assembly, and to compare the effect between the fixing materials and the designs of the magnetic assembly. MATERIAL AND METHODS: Two fixing materials, Jet denture repair acrylic(R) and Super-Bond C&B(R) and two types of magnetic assembly designed with or without wing were used. Each magnetic assembly was fixed in the chamber of the denture base resin block (Lucitone(R)199) with each fixing material respectively. These specimens were thermocycled 2,000 cycles in the water held at 4degrees C and 60degrees C with a dwell time of 1 min each time. Each specimen was seated in a testing jig and then a push-out test was performed with a universal testing machine at a cross head speed of 0.5 mm/min to measure the maximum dislodging forces. RESULTS: Comparing the fixing materials, Super-Bond C&B(R) showed superior dislodging force than Jet denture repair acrylic(R). Comparing the design of the magnetic assemblies, the wing design magnetic assembly showed better dislodging force. Combination of the Super-Bond C&B(R) as a fixing material and wing design magnetic assembly revealed a greatest dislodging force. The kind of fixing material was more influential than the type of magnetic assembly. CONCLUSION: The dislodging force of Super-Bond C&B(R) was significantly higher than Jet denture repair acrylic(R). And the dislodging force of magnetic assembly which have wing design was significantly higher than magnetic assembly which have no wing design.
Boron Compounds
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Collodion
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Denture Bases
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Denture Repair
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Denture, Overlay
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Dentures
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Head
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Humans
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Magnetics
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Magnets
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Methacrylates
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Methylmethacrylates
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Water
8.Implementation of Multiplex PCR for Species Identification and Toxin Typing in Toxigenic Clostridium difficile Culture.
Yun Ha JANG ; Jaewoo CHUNG ; Seungmi BAEK ; Sookja PARK ; Heungsup SUNG ; Mi Na KIM
Korean Journal of Clinical Microbiology 2009;12(1):11-16
BACKGROUND: We evaluated multiplex PCR for species identification and toxin typing to improve the sensitivity and turnaround time of toxigenic Clostridium difficile culture (TCDC). METHODS: We performed multiplex PCR using primers targeting the species-specific gene, tpi, and the toxin genes, tcdA and tcdB. From January to March 2008, 528 stool specimens were tested with direct toxin assay (DT) using C. difficile Tox A/B II (Techlab, Blacksburg, USA) and TCDC. For 288 specimens from early study period, toxin production by C. difficile isolates of TCDC was measured by enzyme immunoassay with culture supernatants using VIDAS C. difficile Toxin A&B (CDAB;bioMerieux, Marcy-l'Etoile, France) and multiplex PCR with isolated colonies. For 240 specimens from late period, only multiplex PCR was used to test toxin production by the isolates. RESULTS: During the early period, 29 C. difficile were isolated and their toxin-positive rates were 65.5% by PCR and 44.8% by CDAB (P<0.05). Among 528 stool specimens, the results of DT+/TCDC+, DT+/ TCDC-, and DT-/TCDC+ were 32 (6.1%), 33 (6.3%), and 10 (1.9%), respectively, when tested with PCR. 13.3% of total 75 positive specimens was detected only by TCDC. Of the 42 toxigenic C. difficile isolates, all were positive for tpi, 30 (71.4%) were tcdA+/tcdB+, and 12 (28.6%) were tcdA-/tcdB+. CONCLUSION: TCDC using multiplex PCR for species identification and toxin typing is sensitive and rapid to be used as a routine diagnostic test.
Boron Compounds
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Clostridium
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Clostridium difficile
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Diagnostic Tests, Routine
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Immunoenzyme Techniques
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Multiplex Polymerase Chain Reaction
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Polymerase Chain Reaction
9.Effect of different treatments of dentin surface on sheer bond strength between different bonding agents and dentin.
Xiao-yan HAN ; Hong-shui ZHU ; Qiu-yue LIU
West China Journal of Stomatology 2008;26(2):125-128
OBJECTIVETo evaluate the effect of Zinc oxide-eugenol cement and Gluma desensitizer on the sheer bond strength of three kinds of dentin bonding agents. The three dentin bonding agents were Zinc phosphate cement, Glass ionomer cement and Super-Bond C&B. To find the theory depending for the using of different protective methods and the selecting of different kinds of dentin bonding agents in prepared abutment teeth.
METHODSThe buccal surfaces of ninety freshly extracted human premolars were flattened to expose an adequate area of lower dentin. Followed by wet-grinding on a series of silicon carbide paper from number 320, 400, 600 grit to produce the dentin bonding surface. The teeth roots were embedded in self-curing resin with the crown out of the resin. The embedded ninety teeth were divided randomly into three groups. The group A was control and the dentin surfaces were not treated. The group B was covered with a paste of Zinc oxide-eugenol cement. The group C was covered with Gluma desensitizer. Calculating the sheer strength between three bonding agents and dentin after the two treatments of dentin surface. The results were statistically assessed with SPSS software. Dentin surfaces were observed with scanning electron microscope (SEM).
RESULTSThe sheer bond strengths of Zinc phosphate cement had significant decrease (P<0.05), especially the C1 group. The sheer bond strengths of Glass ionomer cement and Super-Bond C&B had no significant difference.
CONCLUSIONZinc oxide-eugenol cement and Gluma desensitizer could reduce the sheer bond strength of Zinc phosphate cement with dentin surface. Zinc oxide-eugenol cement and the Gluma desensitizer could not effect Glass ionomer cement and the Super-Bond C&B with dentin.
Boron Compounds ; Crowns ; Dental Bonding ; Dentin ; Dentin-Bonding Agents ; Glutaral ; Humans ; Methacrylates ; Methylmethacrylates ; Resin Cements ; Zinc Oxide-Eugenol Cement
10.Evaluation of Rapid Assay (Tox A/B Quik Chek) for the Detection of Clostridium difficile Toxins A and B.
Sue Jung KIM ; Heejung KIM ; Myung Sook KIM ; Eunmi KOH ; Chang Ki KIM ; Seok Hoon JEONG ; Yunsop CHONG ; Kyungwon LEE
Korean Journal of Clinical Microbiology 2008;11(2):112-116
BACKGROUND: Toxin immunoassay is widely used for rapid diagnosis of Clostridium difficile-associated diarrhea. The aim of this study was to evaluate the performance of Tox A/B Quik Chek test (TECHLAB, Blacksburg, VA, USA) compared to toxigenic culture. METHODS: From September 2006 to August 2007, 959 stools were examined by Tox A/B Quik Chek test and toxigenic culture (C. difficile culture plus tcdB PCR using colonies obtained from culture). RESULTS: Compared to the results of toxigenic culture, the sensitivity and specificity of Tox A/B Quik Chek test were 47.5% and 97.5%, respectively. CONCLUSION: The sensitivity of Tox A/B Quik Chek test was not high, but the specificity was high. Although Tox A/B Quik Chek test alone is not sufficient to diagnose Clostridium difficile-associated diarrhea, it may aid rapid diagnosis, early treatment and prevention of nosocomial spread of the infection, if supplemented by C. difficile culture or tissue culture cytotoxin assay.
Bacterial Proteins
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Bacterial Toxins
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Boron Compounds
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Clostridium
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Clostridium difficile
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Diarrhea
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Early Diagnosis
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Enterotoxins
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Immunoassay
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Immunoenzyme Techniques
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Polymerase Chain Reaction
;
Sensitivity and Specificity