1.Analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.
Lu-Yao HUANG ; Lin-chuan LIAO ; Li-Li CHEN ; Gui SU ; Wen-Jia LI ; Rong HE ; You-Yi YAN ; Lin YANG
Journal of Forensic Medicine 2008;24(6):429-432
OBJECTIVE:
To establish a new method for the analysis of paraquat in blood and urine by sodium borohydride/nickel chloride chemical reduction-gas chromatography/thermionic specific detector.
METHODS:
An initial procedure of precipitation was performed by adding hydrochloric solution with sodium chloride and a mixture of chloroform and ethanol. Then the analyte contained in supernatant was reduced by a reduction system of sodium borohydride and nickel chloride and extracted by acetic ether. Ethyl paraquat (EPQ) was used as internal standard. GC/TSD was used to identify and quantify the analyte.
RESULTS:
The limits of detection (S/N=3) in blood and urine were 0.002 and 0.004 microg/mL, respectively. The linear ranges were 0.050-30.0 microg/mL. Correlation coefficients in blood and urine were 0.999 and 0.998, respectively. The recoveries exceeded 80% both in blood and urine.
CONCLUSION
This method is applicable for quantification of paraquat in biological fluids.
Borohydrides/chemistry*
;
Chromatography, Gas/methods*
;
Forensic Toxicology
;
Herbicides/urine*
;
Humans
;
Nickel/chemistry*
;
Oxidation-Reduction
;
Paraquat/urine*
;
Sensitivity and Specificity