Objective To analyze the difference of the delivery date reckoned by traditional and modified formula for calculating the expected date of confinement (EDC).Methods The data of 2055 women (37-41+6 week) were collected who gave monotocousa term spontaneous birth in the Chongqing Health Center for Women and Children from Jan.2014 to Feb.2015.Of which 1300 were primipara,and 755 were multipara;and the data of 1224 women (39-41week) were collected,of which 832 were primipara,and 392 were multipara.The expected date was calculated with traditional and modified calculating formula,and then the actual delivery date was used for comparison and statistical analysis.Results The coincidence of actual delivery date with the estimated due date reckoned by traditional formula (39-41week) was 8.4％ in primipara and 9.7％ in multipara,and the coincidence reckoned by modified formula was 11.9％ in primipara and 14.8％ in multipara.The EDC estimated by modified formula was more precise than that calculated by traditional formula (P＜0.05).Conclusion The EDC calculated with modified formula is more accurate than that calculated with traditional formula.

Under the principle of giving priority to patient care, efforts were made to expand the functions of HIS in the dispensary of the ward areas. Using HIS to automatically create and print drug-dispensing labels that fit in with clinical needs and thus realizing paperless dispensation in the dispensary of the ward areas could not only reduce errors but also improve work efficiency and service quality. Medication records created automatically with HIS, in the process of which clinical data were automatically entered, were easy to use and provided complete and timely information, thus setting up a sound platform ofr the delivery of pharmaceutical service. A quantitative system for personnel appraisal and control was also established. Hence the enhancement of work efficiency and control level.

Antibiotics being currently the most extensively used medicine in clinical practice and abuse of them being prevalent, it is thus necessary to monitor in an effective and systematic manner the rational use of them. Based on the existing database and network of hospital information system, a monitoring system for the rational use of antibiotics was successfully developed using the development tool of PowerBuild. This functionally powerful and easy-to-use system, which provides comprehensive and reliable information, improved work efficiency and managerial level by automatically monitoring many chief indicators of antibiotic usage, including dosage, resistance, combination with other drugs, course, pathway, usage during the perioperative period, usage for preventive purposes, therapeutic effect, and the ratio of hospital infection occurrence resulting from the use of antibiotics under different circumstances.

Objective To develop an HIS-based electronic medication record. Methods With the aid of the Windows 2000 platform, the clinical data of the patients from the relevant branch systems of HIS was loaded into the medication record management system by using PowerBuild, and then correlative data were processed and corresponding functions were appended. Results An HIS-based electronic medication record was successfully developed. Easy to operate and providing clinical pharmacists with full and accurate clinical data, it could be used both forprospective analyses and retrospective studies. Conclusion As a new mode of medication records, it has sound application prospects in hospitals with good hospital information systems.

Objective To shorten the time of drug reception for ward areas and improve the overall satisfaction level towards the hospital. Methods The process of drug reception for ward areas was optimized by means of information technology and through business process reengineering. Results The time of drug reception for ward areas was effectively shortened and work efficiency and the overall satisfaction level were improved. Conclusion Using information technology and implementing business process reengineering are effective measures that can be taken by hospitals to improve efficiency and satisfaction level.

AIM: To investigate the effect of peripheral administration of arginine vasopressin (AVP) on lipopolysaccharide (LPS)-induced fever and hyperalgesia in rats and its relationship with interleukine-1β (IL-1β) and prostaglandin E2 (PGE2).METHODS: The core temperature (Tc), brown adipose tissue (BAT) temperature and activity were measured by telemetry in adult male Sprague-Dawley rats at an ambient temperature of 23 ℃ during a 12 h light/12 h dark photoperiod (lights on at 06:00 and lights off at 18:00).The rats were intraperitoneally injected with LPS (50 μg/kg), AVP (10 μg/kg) or V1a vasopressin receptor antagonist (V1a antagonist, 30 μg/kg) at 10:00 or 11:30.Hyperalgesia was assessed by measuring the latency to withdraw a hindpaw from radiant heat (Hargreaves test).The concentrations of IL-1β and PGE2 in the serum were tested by ELISA.RESULTS: Intraperitoneal administration of LPS induced periods of biphasic fever accompanied by hyperalgesia.AVP reversed LPS-induced fever, and decreased the hyperalgesia and BAT thermogenesis.Peripheral administration of V1a antagonist enhanced the fever produced by LPS, but did not affect the hyperalgesia.AVP significantly attenuated LPS-induced IL-1β and PGE2 production.CONCLUSION: Peripheral administration of AVP reverses LPS-induced fever and decreases hyperalgesia by reduction of BAT thermogenesis and inhibition of IL-1β and PGE2.Endogenous AVP attenuates the fever induced by LPS, but does not affect the nociceptive thresholds.

Objective To investigate the effect of angiotensin Ⅱ-1 receptor antagonist losartan on expression of tumor necrosis factor-alpha (TNF-?) in the ventricular myocardium in the renovascular hypertension rats. Methods Renovascular hypertension model was obtained by clip left renal artery in Sprague-Dawley(SD) rats. After operation the rats were divided into 3 groups: sham group, two-kidney one clip (2K1C) group, and losartan treatment group(2K1C and losartan 20 mg/kg?d by drinking). Tail blood pressure was determined every week. Animals were euthanized after treatment with losartan for four weeks. Cardiac index(CI)was calculated by HW/BW, and TNF-? protein of ventricle myocardium was determined by ELISA and immunohistochemistry. Results Losartan significantly decreased blood pressure(P

Objective To investigate the antimicrobial and antitumor activities of extracts from the root of mangrove plant,Kandelia candel.Methods Micro-dilution and MTT method were used to test the antimicrobial and antitumor activities of extracts from the root of K.candel.Results The petroleum ether extracts of root could inhibit the growth of Staphylococcus aureus,S.epidermidis,Bacillus subtilis and Sarcina lutea with the minimal inhibitory concentration(MIC) of 3.125 mg?mL-1,1.5625 mg?mL-1,0.78125 mg?mL-1 and 3.125 mg?mL-1 respectively.The ethyl acetate extracts and ethyl alcohol extracts could inhibit the growth of S.epidermidis with MIC of 3.13 mg?mL-1 and 6.25 mg?mL-1 respectively.At the same time,the petroleum ether extracts could inhibit the proliferation of tumor cells,such as SMMC-7721,B16,BGC803 and HepG2 with IC50 of 355.2?g?mL-1,378.6?g?mL-1,275.6?g?mL-1 and 411.6?g?mL-1 respectively,while the other extracts could stimulate the proliferation of tumor cells apparently.Conclusion The antimicrobial and antitumor activities of extracts from the root of K.candel indicate the potential pharmaceutical usage of this plant.

Objective To identify subtypes of human immunodeficiency virus 1(HIV-1)strains from the AIDS patients in Shenzhen and determine whether the HIV-1 subtypes differ in disease progression.Methods HIV-1 env gene was amplified by the nest-RT-PCR from plasma obtained from 26 patients with AIDS in Shenzhen. The C2-V3 regions were sequenced to identify subtypes The plasma viral loads and CD4T lymphocyte were measured as the same time.Results Phylogenetic trees showed that the 12 AIDS patients had subtype B in which, one was close with the U.S reference strain and 11 with the Chinese Yunnan reference strain;13 AIDS patients had subtype CRF01-AE from Thailand;There were no differences in the CD4 cell count and plasma HIV-RNA levels between individuals infected with subtypes B and CRF01-AE.Conclusion Our study indicated that HIV-1 subtype B and CRF01-AE strains were present in AIDS patients in Shenzhen. There was no evidence that the subtypes of virus could determine disease progression.

AIM:Toinvestigatetheeffectofrhynchophylline(Rhy)onbloodpressure,cardiachypertrophy and myocardial fibrosis in spontaneously hypertensive rats ( SHR) .METHODS:Spontaneously hypertensive rats were ran-domly divided into model group , high dose (10 mg? kg-1? d-1 ) and low dose (2.5 mg? kg-1? d-1 ) group of rhyncho-phylline, captopril group (17.5 mg? kg -1? d-1).Wistar-Kyoto rats were used as normal control.Respectively, systolic blood pressure was measured by tail cuff every 2 weeks.After 10 weeks, heart weight index and left ventricular weight in-dex were calculated .The myocardial hydroxyproline and plasma angiotensin Ⅱwere detected .Moreover , basic myocardial histopathological changes and myocardial collagen fibres were observed by HE staining and Masson staining , respectively . The protein expression of TGF-β1 and Smad3 in the myocardium was measured by the methods of immunohistochemistry and Western blot .RESULTS:Compared with SHR model group , Rhy significantly reduced blood pressure ( P<0.05 ) , the levels of HYP in the myocardium (P<0.05) and the levels of AngⅡin the plasma (P<0.01).The pathological dama-ges of the myocardial tissues and collagen deposition were attenuated .The protein expression of TGF-β1 and Smad3 was sig-nificantly reduced by the treatment with Rhy (P<0.01).CONCLUSION:Rhynchophylline reduces blood pressure and adjusts to improve ventricular remodeling of SHR .The mechanism may be involved in the TGF-β1/Smad pathway and re-ducing AngⅡcontent.