1.Relation between lymphocyte subpopulations of peripheral blood and immune responses of modified live hog cholera virus vaccine in pigs treated with an ionized alkali mineral complex.
Bong Kyun PARK ; Yong Ho PARK ; Kyung Suk SEO
Journal of Veterinary Science 2000;1(1):49-52
Thirty-nine healthy pigs (28-32 days old) were purchased from a commercial swine farm and housed at swine pens of the College. The animals were vaccinated intramuscularly (1 ml) with an attenuated live hog cholera virus (HCV, LOM strain) and then boostered at 5 weeks after the first vaccination. The animals were divided into 4 experimental groups: 0.05% (w/w) PowerFeel-supplemented diet (T-1, n = 10); 3% (w/w) SuperFeed-supplemented diet (T-2, n = 10); diluted PowerFeel solution (1 : 500, v/v) as drinking water (T-3, n=9); control (n=10). PowerFeel is an original form of ionized alkali mineral complex (IAMC) and SuperFeed is a commercial product of IAMC. The subpopulation of lymphocyte in blood was assayed by a flow cytometry and HCV-specific antibody was determined by an indirect immunofluorescence assay. In IMAC-treated groups, the proportions of subpopulation expressing MHC-class II, CD2+, CD4+, CD8+, and surface IgM+ B lymphocytes were significantly decreased at 5-weeks after the first vaccination. Significant decreases were also observed in the proportions of MHC-class II, CD2+ and CD8+ lymphocyte at 3-weeks after the booster injection. The humoral immune responses in T-1 and T-2 groups were greater than those in T-3 or control group. These results suggest that IAMC-supplemented diets may have an HCV-specific immunostimulatory effect in pigs.
Animal Feed
;
Animals
;
Antibodies, Monoclonal/*blood/isolation & purification
;
Antigens, CD2/blood
;
B-Lymphocytes/immunology
;
CD4-Positive T-Lymphocytes/immunology
;
CD8-Positive T-Lymphocytes/immunology
;
Classical Swine Fever/*immunology
;
Classical swine fever virus/*immunology
;
Dietary Supplements
;
Ions
;
Lymphocyte Subsets/*immunology
;
*Minerals
;
Swine
;
Vaccines, Attenuated/*administration & dosage
;
Viral Vaccines/*administration & dosage
2.A Case of Antenatally Diagnosed Fetal Ovarian Cyst.
Chi Hun SONG ; Seo Yoo HONG ; Soo Mi CHUNG ; Kyung Chul HAN ; Chul Bum PARK ; Bong Gyu SHIN
Korean Journal of Obstetrics and Gynecology 1999;42(3):651-655
Ovarian cysts in the newborn are uncormnon. Fetal ovarian cysts are being diagnosed with inaeasing fiequency with development of obstetrical sonography. Most of these cysts are found within the first few months of life and have no clinical significance. But, a large ovarian cyst would be founded antenatally with sonography. A large ovarian cyst can cause life-theatening complications during parturition and in the neonatal period. We have experienced a case of fetal ovarian cyst in a 32-year-old primiparous woman with antenatal sonography and report our experience with a brief review of literatures.
Adult
;
Female
;
Humans
;
Infant, Newborn
;
Ovarian Cysts*
;
Parturition
;
Ultrasonography
3.One Case of Insulinoma.
Jae Hee AHN ; Hye Sang SEO ; Sung Chul YOON ; Kyung Woo YOON ; Soo Bong CHOI ; Hyun Woo LEE
Yeungnam University Journal of Medicine 1988;5(2):205-211
Insulinoma is a rare tumor, occurring more often in women and in the older age range. Eighty percent of patients have a single benign tumor, usually 2 cm in diameter, located with about equal frequency in body, head or tail of pancreas and amenable to surgical cure. About 10% have multiple tumors. The remaining 10% of patients have metastatic malignant insulinoma. The symptom of insulinoma is characterized by the periodic attack of hypoglycemia of blood sugar level below 50mg%, by fasting or exertion, and rapid relief of symptom by oral or intravenous administration of glucose. Symptom often lead to misdiagnosis as a neurologic or psychiatric disorder. A case described by authors was 44-year old female with the chief complaints of the loss of consciousness, epileptic seizure although she has been treated by anticonvulsants. Serum blood sugar and insulin level during fasting suggested insulinoma but abdominal computed tomography shows no definitive mass in pancreas. Celiac angiography revealed insulinoma. She transferred to the department of General Surgery and was performed enucleation. Microscopic findings shows the islet cell tumor of pancreas. A brief review of the literature was made.
Adenoma, Islet Cell
;
Administration, Intravenous
;
Angiography
;
Anticonvulsants
;
Blood Glucose
;
Diagnostic Errors
;
Epilepsy
;
Fasting
;
Female
;
Glucose
;
Head
;
Humans
;
Hypoglycemia
;
Insulin
;
Insulinoma*
;
Pancreas
;
Tail
;
Unconsciousness
4.The development of Genu Valgum of the Affected Limb in Legg-Calve-Perthes Disease.
Sung Man ROWE ; Sung Taek JUNG ; Hyoung Yeon SEO ; Bong Hyun BAE ; Myung Sun KIM ; Kyung Soon PARK
Journal of the Korean Hip Society 2006;18(4):173-181
Purpose: Genu valgum in Legg-Calve-Perthes disease (LCPD) is not a known complication. We investigated whether this valgus tendency is a complication of femoral varus osteotomy (FVO) or the sequela of the disease process itself, and what are the related factors. Materials and Methods: 35 patients treated by FVO and 38 by abduction orthosis (AO) were selected for this study. Only those patients with unilateral involvement, fragmentation stage, Catterall Group III or IV, and who were followed-up to full skeletal maturity were included in the study. We measured mechanical axis deviation percentage and hip-knee-ankle angle for femorotibial alignment, and mechanical lateral distal femoral angle and medial proximal tibial angle (mMPTA) for knee orientation on teleoroentgenograms and compared the affected and normal limbs. Results: All measurements, excluding the mMPTA, demonstrated a tendency of relative genu valgum versus the opposite normal limbs. This tendency was observed in both groups. There was no statistically significant difference between the two groups. Conclusion: Genu valgum occurred in 66% to 70% of the LCPD patients. Four factors were found to be significantly correlated with valgus shift: increased medial bowing of the femoral neck, decreased acetabulum head index, coxa magna, and limb shortening. Multivariate regression analysis identified limb shortening as the factor most responsible for valgus shift.
Acetabulum
;
Axis, Cervical Vertebra
;
Extremities*
;
Femur Neck
;
Genu Valgum*
;
Head
;
Hip
;
Humans
;
Knee
;
Legg-Calve-Perthes Disease*
;
Orthotic Devices
;
Osteotomy
5.Ultrastructural and Immunohistochemical Study of Hepatic Fibrosis after the Ligation of the Common Bile Duct in Rats.
Kyung Rye MOON ; Young ILL ; Woo Chul SEO ; Yeong Bong PARK ; Man Woo KIM ; Jae Hong SEO ; Sang Kee PARK
Korean Journal of Pediatric Gastroenterology and Nutrition 1999;2(2):185-193
PURPOSE: Proliferation of bile duct-like structures and fibrosis is a hepatic cellular reaction observed in most forms of human liver disease and in a variety of experimental conditions associated with liver injury. The aim of this study was to investigate the activation of Ito cells and bile duct proliferation in the rat after common bile duct ligation (CBDL). METHODS: Hepatic morphological abnormalities were examined in rats whose bile ducts had been irreversibly ligated for 15, 21, 24 and 28 days. The liver was examined by immunohistochemical staining for alpha-smooth muscle actin, the known marker of activated Ito cells, and light and electron microscopes. RESULTS: After CBDL, the bile canalicular proliferation and interstitial fibrosis were gradually increased in the periportal areas extended to hepatic sinusoids. Ito cells positive for alpha-smooth muscle actin were frequently observed in the periductular space and in perisinusoidal space of Disse. Ito cells and myofibroblasts were gradually increased in the interstitial fibrosis until the 28th day after CBDL. Ito cells and myofibroblasts had microfilaments with dense body at the periphery of the cell. CONCLUSIONS: Our results suggest that Ito cells may be fibroblastic or myogenic. It has also been postulated that during the development of hepatic fibrosis, Ito cells become myofibroblasts or fibroblast like cells.
Actin Cytoskeleton
;
Actins
;
Animals
;
Bile
;
Bile Ducts
;
Common Bile Duct*
;
Fibroblasts
;
Fibrosis*
;
Hepatic Stellate Cells
;
Humans
;
Ligation*
;
Liver
;
Liver Diseases
;
Myofibroblasts
;
Rats*
6.Ejaculatory Failure after Unilateral Neurolytic Celiac Plexus Block.
Seo Kyung SHIN ; Tae Dong KWEON ; Sang Hee HA ; Kyung Bong YOON
The Korean Journal of Pain 2010;23(4):274-277
Abdominal pain associated with chronic pancreatitis is often difficult to control with analgesics and can be severely debilitating with significant impairment of quality of life. In these patients, neurolytic celiac plexus block (NCPB) is an effective treatment option with a low complication rate. However, there is a risk of ejaculatory failure after NCPB, which may be a problem in patients with a long life expectancy. We report a case of ejaculatory failure after unilateral NCPB in a patient with chronic pancreatitis.
Abdominal Pain
;
Analgesics
;
Celiac Plexus
;
Humans
;
Life Expectancy
;
Pancreatitis, Chronic
;
Quality of Life
7.Effect of Extracorporeal Shockwave Therapy Versus Intra-articular Injections of Hyaluronic Acid for the Treatment of Knee Osteoarthritis.
June Kyung LEE ; Bong Yeon LEE ; Woo Yong SHIN ; Min Ji AN ; Kwang Ik JUNG ; Seo Ra YOON
Annals of Rehabilitation Medicine 2017;41(5):828-835
OBJECTIVE: To evaluate and compare the effects and outcomes of extracorporeal shock wave therapy (ESWT) and intra-articular injections of hyaluronic acid (HA) in patients with knee osteoarthritis (OA). METHODS: Of the 78 patients recruited for the study, 61 patients met the inclusion criteria. The enrolled patients were randomly divided into two groups: the ESWT group and the HA group. The ESWT group underwent 3 sessions of 1,000 shockwave pulses performed on the affected knee with the dosage adjusted to 0.05 mJ/mm² energy. The HA group was administered intra-articular HA once a week for 3 weeks with a 1-week interval between each treatment. The results were measured with the visual analogue scale (VAS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), Lequesne index, 40-m fast-paced walk test, and stair-climb test (SCT). A baseline for each test was measured before treatment and then the effects of the treatments were measured by each test at 1 and 3 months after treatment. RESULTS: In both groups, the scores of the VAS, WOMAC, Lequesne index, 40-m fast-paced walk test, and SCT were significantly improved in a time-dependent manner (p<0.01). There were no statistically significant differences measured at 1 and 3 months after treatment between the two groups (p>0.05). CONCLUSION: The ESWT can be an alternative treatment to reduce pain and improve physical functions in patients with knee OA.
High-Energy Shock Waves
;
Humans
;
Hyaluronic Acid*
;
Injections, Intra-Articular*
;
Knee*
;
Ontario
;
Osteoarthritis
;
Osteoarthritis, Knee*
;
Shock
9.Effects of Repetitive Ischemic Preconditioning on the Expression of Nitric Oxide Synthase in Tibialis Anterior and Soleus Muscles of the Rat.
Bong Cheol KOH ; Youn Kyoung SEO ; Chu Ok SHIN ; Soo Kyung CHUN ; Doo Jin PAIK
Korean Journal of Physical Anthropology 2007;20(3):213-224
Nitric oxide synthases (NOSs) that catalyzed the conversion of L-arginine to nitric oxide and L-citrulline play a role in ischemic-reperfusion injury. The purpose of this study was to observe the expression patterns of nNOS, iNOS and eNOS in the rat tibialis anterior and soleus muscles after multiple cyclic episodes of ischemic preconditioning (IP). Nine weeks old male SD rats were divided into control and IP groups. The IP group was further divided into 3 groups based on cycle of IP. For IP, left commom iliac artery was occluded 3, 6 and 10 times for 5 minutes ischemia followed by 5 minutes reperfusion using rodent vascular clamps. The animals were sacrificed at 0, 3, 6, 24 and 72 hours of reperfusion and the left tibialis anterior and soleus muscles were removed. The expression of nNOS, iNOS and eNOS were examined with immunohistochemical methods and Western blot analysis. IP increased the expression of nNOS, compared with the control. In the tibialis anterior muscle, the levels of nNOS in the 3IP and 6IP were higher than that in 10IP. IP increased the expression of iNOS, compared with the control, and the levels of iNOS in tibialis anterior muscle were higher than that in soleus muscle. The level of iNOS in the 10IP was higher than those in the 3IP and 6IP. IP increased the expression of eNOS, compared with the control, and the level of eNOS in soleus muscle were higher than that in tibialis anterior muscle. At 0 and 3 hours after reperfusion, the level of eNOS in 6IP and 10IP were higer than that in 3IP. In summary, these results suggest that the ischemic preconditioning increases the expression of nNOS, iNOS and eNOS, and 10 times of ischemic preconditioning may induce ischemic injury through upregulation of iNOS. And tibialis anterior muscle is more susceptabile to ischemic injury than soleus muscle.
Animals
;
Arginine
;
Blotting, Western
;
Humans
;
Iliac Artery
;
Ischemia
;
Ischemic Preconditioning*
;
Male
;
Muscle, Skeletal
;
Muscles*
;
Nitric Oxide Synthase*
;
Nitric Oxide*
;
Rats*
;
Reperfusion
;
Rodentia
;
Up-Regulation
10.Volume Changes of Frontal Lobe and Hippocampus in Juvenile Myoclonic Epilepsy.
Woo Suk TAE ; Eun Kyung LEE ; Eun Yeon JOO ; Dae Won SEO ; Seung Bong HONG
Journal of the Korean Neurological Association 2003;21(1):54-61
BACKGROUND: In order to investigate the structural abnormalities in juvenile myoclonic epilepsy (JME), the volumes of the hippocampus and frontal lobe were measured. METHODS: Nineteen JME patients and 19 age- and sex-matched normal controls underwent a 1.6 mm thick brain SPGR MRI. The volumes of the frontal lobe and hippocampus were measured with a volume of interest method. The differences of volumes between JME and normal groups were compared. The volumes were also compared between the early and late onset groups, and between the short and long seizure duration groups. The correlations between the volumes of the frontal lobe, hippocampus, or mid-sagittal area of the corpus callosum, and the age of seizure onset or the duration of disease were tested. RESULTS: The cerebral volume was not found to be different between the JME and normal groups (p=0.521). The left hippocampus was significantly smaller in the JME group (p=0.032) while the left frontal lobe was significantly larger in the JME group (p=0.004). The area of the rostral body of corpus callosum showed a significant positive correlation with the age of onset (r=0.561, p=0.012). The right frontal lobe volume showed a significant negative correlation with the duration of disease (r=-0.511, p=0.025). CONCLUSIONS: These results imply that JME has volume changes in the frontal lobe and hippocampus. The volume reduction of the right frontal lobe in patients with a longer disease duration may suggest a progressive nature of JME. The smaller rostral body of the corpus callosum in patients with earlier seizure onset may suggest an ontogenic abnormality of JME.
Age of Onset
;
Brain
;
Corpus Callosum
;
Frontal Lobe*
;
Hippocampus*
;
Humans
;
Magnetic Resonance Imaging
;
Myoclonic Epilepsy, Juvenile*
;
Seizures