To recognize the hemodynamic change in the constrictive pericarditis, we have reviewed the Doppler echocardiography, cardiac catheterization, and pathology of 6 patients who were admitted to Pusan National University Hospital due to right-side heart failure, diagnosed as constrictive pericarditis and were undertaken pericardiectomy. Doppler echocardiographic findings showed that acceleration and deceleration of early diastolic rapid filling were increased, followed by shortening of duration but there was a tendency to decrease in velocity-time integral of early diastolic rapid filling compared to that of atrial contraction filling in the left ventricle. On the contrary, there was decrease in acceleration of rapid filling in right ventricle, but other indices were comparable to that of left ventricle. The integral of D wave increased relatively compared to that of S wave in superior vena canal flow. Also, there was decrease in peak flow velocity, acceleration and velocity-time integral of aortic and pulmonary arterial flow velocity. One patient who had increased fraction of integral of early diastolic rapid filling compared to that of atrial contraction filling in right ventricle showed that he had higher central venous pressure and D wave was dominant in superior vena caval flow. In conclusion, Doppler flow patterns showed characteristic diastolic filling inpairment and systolic dysfunction in constrictive pericarditis, so that Doppler echocardiography is believed to be a useful method of noninvasive diagnosis and follow-up of hemodynamic change in constrictive pericarditis.
Acceleration ; Busan ; Cardiac Catheterization ; Cardiac Catheters ; Central Venous Pressure ; Deceleration ; Diagnosis ; Echocardiography ; Echocardiography, Doppler ; Follow-Up Studies ; Heart Failure ; Heart Ventricles ; Hemodynamics ; Humans ; Pathology ; Pericardiectomy ; Pericarditis, Constrictive*

A noninvasive method for assessing cardiac output was evaluated by comparing it with thermodilution determinations in 25 patients who admitted to Pusan national university hospital from March, 1985 to December, 1986. This method used M-mode & two dimensional echocardiography to measure the internal diameter of aortic valve anulus & pulmonary valve anulus and pulsed doppler echocardiography to obtain aortic & pulmonary blood velocity. Good correlations were observed between thermodilution and doppler echocardiographic measurements of cardiac output from aortic flow (r=0.98, p<0.05) & pulmonary flow (r=0.86, p<0.05). Linear regression analysis yielded y=0.91x0.14 for aortic flow and y=0.77x0.84 for pulmonary flow. These results indicate that accurate cardiac output can be measured by noninvasive & simple doppler echocardiography.
Aortic Valve ; Busan ; Cardiac Output* ; Echocardiography* ; Echocardiography, Doppler ; Echocardiography, Doppler, Pulsed ; Humans ; Linear Models ; Pulmonary Valve ; Thermodilution

Lymphoma forms the largest group of lymphoproliferative disorder in the orbit. Orbit CT scan and biopsy are very helpful for diagnosis. Main treatment is radiotherapy and chemotherapy. The authors experienced a case of orbital lymphoma in a 45-year-old male who visited our hospital due to proptosis of the right eye. Histopathologic result showed diffuse small lymphocytic Non-Hodgkin's lymphoma. We present this case and discuss its management.
Biopsy ; Diagnosis ; Drug Therapy ; Exophthalmos ; Humans ; Lymphoma* ; Lymphoma, Non-Hodgkin ; Lymphoproliferative Disorders ; Male ; Middle Aged ; Orbit* ; Radiotherapy ; Tomography, X-Ray Computed

STATEMENT OF PROBLEMS: The concept of CR has also changed continuously. In order to find out the factors that affect the centric slide, studies were carried out to compare the forms of wisdom teeth eruption, lateral movement, premature contact in CR, and anterior movement. Research and statistical methods were based on the report by the 1980 Korean dental association. MATERIAL AND METHOD: In our study, 403 dentists in their twenties and dentistry students who could understand CR and CO (and who did not receive occlusal, orthodontic treatment, without extreme caries and large prosthodontic care) were compared with the 25 year old results. A segment of line parallel to the upper incisor was marked on the lower incisor. When seen laterally, a line perpendicular to the occlusal plane was drawn on the foremost area of the upper incisor. This line was extended to the lower incisor and the two points (points at the lower and upper incisors) were used as reference points for the CO. After guiding the occlusion to the CR, two lines were marked by using the same method that was used for the CO. The point in which these lines meet became the reference point of CR occlusion. RESULTS AND CONCLUSIONS: Results of the experiment completed in 1980 show that all 307 research members had anterior-posterior and upper-lower displacement. Displacement measurements were 0.7+/-0.4 mm for the anterior-posterior displacement, 0.99+/-0.50 mm for the upper-lower displacement,0.18+/-0.31 mm for the lateral displacement, and 1.32+/-0.67 mm for the total displacement. Results of the 2006 experiment show that all 409 research members had anterior-posterior and upper-lower displacement. The anterior-posterior displacement was 1.12+/-0.86 mm, the upper-lower displacement was 1.02+/-0.71 mm, the lateral displacement was 0.61+/-0.56 mm, and the total displacement was 1.80+/-0.99 mm. No specific differences were found between each group when comparing displacement according to the forms of wisdom teeth eruption. Since 1980, the percentage of unerupted teeth has increased from 35.16% (111/307 people) to 57.5% (236/409 people). Westernization of the Korean cranial form and intraoral structure has brought about these results. In our experiment, 26.7% (109/409 people) of the subjects were cuspid guided, while 7.3% (30/409 people) were mutually guided. No specific differences were found in the amount of displacement between the two groups. Only the subjects with anteriors coming in contact made up the largest percentage group (42.3%, 173/409 people) in our study. No specific differences were found between each group.
Adult ; Cuspid ; Dental Occlusion ; Dentistry ; Dentists ; Humans ; Incisor ; Molar, Third ; Prosthodontics ; Tooth, Unerupted

We report a clinical ananlysis of children's perforating eye injuries which happened in middle western of Korea from Jan., 1988 to Apr., 1993. Total number of patients with perforating eye injuries was 40(40 eyes). The peak age group was 5-6 year of age. The cornea was the most common site of perforation. The most frequent cause of perforating eye injuries was knife and scissors(17.5%) and sharp pointed iron material(17.5%) and followed by glass(17.5%), wood(17.5%), pencil 7.5%), toy(5%), traffic accident(5%). According to this result obtained, the visual prognosis of perforating eye injuries in children is still poor. Special care should be taken to prevent such a severe eye trauma in children by every means.
Child ; Cornea ; Eye Injuries* ; Humans ; Iron ; Korea* ; Prognosis

BACKGROUND: Lung cancer is one of the leading causes of cancer-related deaths throughout the world. The gene tumor protein 53 (TP53) is frequently mutated in cases of lung cancer. This study was performed to investigate the frequencies and types of mutations in the TP53 gene in Korean patients with lung cancer. METHODS: We obtained tissue samples from 80 lung cancer patients and synthesized TP53 cDNA by using RNA isolated from these tissues by performing reverse transcriptase polymerase chain reaction. Hybridization and denaturing high-performance liquid chromatography were performed to identify the TP53 gene mutations, and then, the mutations were validated by direct sequencing. RESULTS: Forty mutations out of the 80 patients (50.0%) were noted in the TP53 gene. The frequencies of TP53 gene mutation for different cancer types, namely, squamous cell carcinoma, adenocarcinoma, and small cell carcinoma were 61.1%, 27.3%, and 26.7%, respectively. The mutation frequencies in the different regions of the gene were 10.0% for exon 4, 35.0% for exon 5, 12.5% for exon 6, 22.5% for exon 7, 17.5% for exon 8, and 2.5% for exon 9. The frequently mutated positions were codon 179 in exon 5, codons 202 and 220 in exon 6, and codons 266 and 273 in exon 8. CONCLUSIONS: Exon 5 was the most frequently mutated region in the TP53 gene. Compared to the patients with the other types of cancers, patients with squamous cell carcinoma showed a higher frequency of TP53 mutation. Codon 179 was the most frequently mutated codon in the TP53 gene.
Adenocarcinoma ; Carcinoma, Small Cell ; Carcinoma, Squamous Cell ; Chimera ; Chromatography, Liquid ; Codon ; DNA, Complementary ; Exons ; Genes, p53 ; Humans ; Lung ; Lung Neoplasms ; Mutation Rate ; Reverse Transcriptase Polymerase Chain Reaction ; RNA

We analyzed the practical problems and factors which affect making the correct differential diagnosis in the interpretation or test results from the nocturnal penile tumescence (NPT) test and erotic stimulation test (EST). This was done to provide better information for higher diagnostic accuracy in the clinical application of these tests. The followings are the results of NPT tests and EST identifying the factors affecting correct differential diagnosis by comparison the other differential diagnostic methods. The overall sensitivity of NPT test (Number: 114 total patients) was 82%. 21 cases (18%) could not be diagnosed correctly due to traction of the sensor (12 cases, 10%) and sleep disturbance (9 cases, 8%). The overall sensitivity of EST (Number: 174 total patients) without considering the degree of patient`s sexual drive to erotic stimulation was 77%. 40 cases (23%) could not be diagnosed correctly due to tolerance to pornographic film (17 cases, 10 %), discomfort by the body attachments (14 cases, 8%) and traction of the sensor (9 cases, 5%). However, higher sensitivity (90%) and lower rate of incorrect diagnosis (10% ) were observed in 119 patients who showed Grade II or III (moderate to good) sexual drive to erotic stimulation. The results suggest that undesirable factors in the primary screening methods, traction of sensor, sleep disturbance in NPT test, and tolerance to pornographic film, discomfort by the body attachments, traction of sensor in EST must be taken into consideration when interpretation of test results is being performed.
Diagnosis ; Diagnosis, Differential ; Diagnostic Tests, Routine* ; Erectile Dysfunction* ; Humans ; Male ; Mass Screening ; Penile Erection ; Traction

Human gingival fibroblasts have proven to useful as a species specific cell culture system in various system on periodontal disease and regeneration. However, their use is limited, since they are hard to obtain and lifespan is short due to replicative senescence. To overcome these disadvantages, we transfected primary human gingival fibroblasts by the E6 and E7 genes of the Human papilloma virus(HPV) 16. The full length of HPV 16 E6 and E7 was cloned from the pBR322 into BamH1 and Sal I of a pBabe vector including hygromycin B resistance. Before pBabeE6/E7 plasmid transfection, peak 8 GFP including G418 resistance was transfected into primary GF to check the transfection efficency. PBabe E6/E7 plasmid was transfected using Lipofectamine plus following manufacter's instruction into primary normal human gingival fibroblasts in 60mm dishes with FBS free DMEM. After 2 days of transfection, the cells were treated with hygromycin for 2 weeks until the transfected control cells died. The resulting hygromycin resistant colonies were pooled, and clonned, and sucessful transfection was established for immortalized gingival fibroblast cell lines. Immoralized GF cells showed stellate shape, that is similar to that of orange grains, and more rapid growth and higher proliferation than that of primary gingival fibroblasts. This cell lines overcame crisis and could be cultured over 30 subcultured, could be use for three dimentional culture, epithelial-mesenchymal interaction study.
Cell Aging ; Cell Culture Techniques ; Cell Line* ; Edible Grain ; Citrus sinensis ; Clone Cells ; Fibroblasts* ; Human papillomavirus 16 ; Humans* ; Hygromycin B ; Papilloma ; Periodontal Diseases ; Plasmids ; Regeneration ; Transfection

In order to observe the change of the clinical results after Microvascular Decompression(MVD), we followed up 261 cases with hemifacial spasm(HFS). The patients were operated between Nov. 1985 and Aug. 1994. The length of observation had been more than 6 months in all cases. The mean age of the patients was 47.0 years old and the mean duration of symptom was 8.2 years. Female constitutes 82.8% of the total an right side was involved in 52.1%. The most common offender was the anterior inferior cerebellar artery (51.0%), and 33% involved multiple vessels. The most common offender was the anterior inferior cerebellar artery (51.0%), and 33% involved multiple vessels. The postoperative complications occurred in 84 cases, 4 of which were permanent. However, there were no mortalities. Among the 226 cases, which were followed up for more than 6 months after the MVD, the final clinical results of 187 cases were excellent, the success rate was 91.7%, and the patients' satisfaction rate was 96.1%. The rate of excellence was 61.1% at POD 7th and 82.7% at final, respectively. The delayed resolution of the HFS during follow-up comprised 37.4% of the completely resolved cases. According to the above results, the clinical status of HFS after MVD continuously changes, therefore follow-up observation after MVD is needed to evaluate the operative results, especially in the delayed resolved cases.
Arteries ; Criminals ; Female ; Follow-Up Studies ; Hemifacial Spasm* ; Humans ; Microvascular Decompression Surgery* ; Mortality ; Postoperative Complications ; Prospective Studies*

BACKGROUND: To enhance the cancer cell detection rate in blood, we tried to detect cancer cells by blood filtration, RNA extraction and reverse transcription (RT)-PCR in the filtered mononuclear cells (MNCs). METHODS: From the specimens of whole blood and filtered MNCs, RNA was extracted by the guanidinium isothiocyanate buffer method. Filtration efficiency was evaluated by measurement of leukocyte count, red cell count, and hemoglobin level. To compare the RNA extraction efficiency between whole blood and filtered MNCs, the followings were examined: (1) RNA electrophoresis, (2) hTERT, survivin, plakophilin, LunX, and MAGE A1-6 RT-PCR, and (3) the detection limit of added SNU484 cells in the blood by MAGE A1-6 RT-PCR. Finally blood specimens of 13 lung cancer patients were used to detect cancer cells by LunX and MAGE A1-6 RT-PCR with filtered MNCs. RESULTS: The filtration method revealed 0%, 92.8% and 95.1% filtration rates for leukocyte, red cell, and hemoglobin, respectively. Contamination of concentrated genomic DNA was observed in the electrophoresis of RNA extracted from whole blood. Positive rates of hTERT, survivin and plakophilin were higher in the filtered MNCs. The filtration method detected 1 SNU484 cell/mL, and the blood samples of 4 (30.8%) lung cancer patients showed positive results for RT-PCR. CONCLUSIONS: For the detection of cancer cells in the blood, the filtration method was very efficient, and LunX and MAGE A1-6 genes would be useful for the detection of blood cancer cells in patients with lung cancer.
Cell Count ; DNA ; Electrophoresis ; Filtration ; Guanidine ; Hemoglobins ; Humans ; Isothiocyanates ; Leukocyte Count ; Leukocytes ; Limit of Detection ; Lung Neoplasms ; Reverse Transcription ; RNA