1.Some distribution features of the hematopoietic and immunological cells in the bone marrow in 38 Vietnamse healthy people
Journal of Vietnamese Medicine 2002;269(2):6-11
38 Vietnamese adult healthy people studied on peripheral blood and bone marrow material to investigate on hemopoiesis and distribution of blood cells as well as lymphocytic subsets. The results showed that in peripheral blood, the hematologic and lymphocytic count parameters are sames as medical literature. In bone marrow material, nucleated cell count is in strict range with mean value of 54+/- 16G/L. The three common lines of bone marrow are granulocytic, erythrocytic and lymphocytic lines are distributed in distribution of lymphocytic subsets in bone marrow in comparison to peripheral blood. All subsets are more higher than that of peripheral bood about 2.5 to 4 time times with conversely ratio CD4/8.
Bone Marrow
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Blood Cells
2.A case of idiopathic hyperoeosinophilic syndrome with increased promyelocytes in bone marrow finding.
Yeon Suk KIM ; Jee Yung AHN ; Hwi Jun KIM ; Soon Kil KIM ; Seung Ho SHIN ; Seung Ho BAEK ; Chang Jin KIM
Korean Journal of Hematology 1992;27(2):331-337
No abstract available.
Bone Marrow*
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Granulocyte Precursor Cells*
3.Bone Marrow Cell Culture(GM-CFU) in Anaplastic Anemia of Children.
Journal of the Korean Pediatric Society 1985;28(9):888-898
No abstract available.
Anemia*
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Bone Marrow Cells*
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Bone Marrow*
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Child*
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Humans
4.Fine structures of bone marrow cells in cyclosporine: A treated mouse.
Young Ho LEE ; Tae Kyoung PAIK ; Ho Sam CHUNG ; Kyu Sik LEE
Korean Journal of Hematology 1993;28(1):47-54
No abstract available.
Animals
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Bone Marrow Cells*
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Bone Marrow*
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Cyclosporine*
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Mice*
5.Sequential changes of bone marrow pathology and BFU-E in recipients of allogenic bone marrow transplantation.
Jong Hyun YOON ; Han Ik CHO ; Sang In KIM ; Byeong Kook KIM ; Seonyang PARK ; Noe Kyeong KIM ; Munho LEE
Korean Journal of Hematology 1992;27(1):23-32
No abstract available.
Bone Marrow Transplantation*
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Bone Marrow*
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Erythroid Precursor Cells*
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Pathology*
6.Significance of Bone Marrow Unclassifiable Cells in Diagnosis of Fever of Unknown Origin.
Juan ZHAO ; Wen-Juan WANG ; Ning FU ; Yun-Ru CHEN ; Ya-Lin LIU
Journal of Experimental Hematology 2019;27(6):1845-1849
OBJECTIVE:
To investigate the clinical significance of bone marrow unclassifiable cells in diagnosis of fever of unknown origin(FUO).
METHODS:
The clinical data of 60 patients with FUO admitted in the first affiliated hospital of Xi'an Jiaotong university from June 2014 to May 2016 were collected, and 60 patients with FUO were divided into 2 group: group A(30 cases) in which the unclassifiable cells in bone marrow were observed by bone marrow examination, and group B(30 cases) in which the unclassifiable cells in bone marrow not were found by bone marrow examination. The clinical characteristics, bone marrow features, immunophenotypes of bone marrow cells and prognosis of patients in 2 groups were analyzed retrospectively.
RESULTS:
Out of 30 patients in group A, 18 were diagnosed as malignant tumors including 12 cases of lymphoma, while out of 30 patients in group B, 5 cases were diagnosed as malignant tumor, including 3 cases of lymphoma. For the patients with non-tumor diseases, the bone marrow unclassifiable cells disappeared after the patients condition was improved.
CONCLUSION
The bone marrow examination including the smear and biopsy shonld be performed routinely for the patients with FUO. If the unclassifiable cells are observed morphologically in bone marrow of patients with FUO, the disease of patients should be considered as malignant tumor, especially, lymphoma.
Bone Marrow
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Bone Marrow Cells
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Bone Marrow Examination
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Fever of Unknown Origin
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Humans
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Retrospective Studies
7.Establishment of a platelet production model by bone marrow cavity transplantation of mouse primary megakaryocytes.
Bai Ming HUANG ; Xiao Yuan CHEN ; Mei Juan XIA ; Lin ZHENG ; Cui Cui LIU ; Jing Jing ZHAO ; Pei SU ; Hong Tao WANG ; Jia Xi ZHOU
Chinese Journal of Hematology 2022;43(4):272-278
Objective: To establish an intramedullary transplantation model of primary megakaryocytes to evaluate the platelet-producing capacity of megakaryocytes and explore the underlying regulatory mechanisms. Methods: Donor megakaryocytes from GFP-transgenic mice bone marrow were enriched by magnetic beads. The platelet-producing model was established by intramedullary injection to recipient mice that underwent half-lethal dose irradiation 1 week in advance. Donor-derived megakaryocytes and platelets were detected by immunofluorescence staining and flow cytometry. Results: The proportion of megakaryocytes in the enriched sample for transplantation was 40 to 50 times higher than that in conventional bone marrow. After intramedullary transplantation, donor-derived megakaryocytes successfully implanted in the medullary cavity of the recipient and produce platelets, which showed similar expression of surface markers and morphology to recipient-derived platelets. Conclusion: We successfully established an in vivo platelet-producing model of primary megakaryocytes using magnetic-bead enrichment and intramedullary injection, which objectively reflects the platelet-producing capacity of megakaryocytes in the bone marrow.
Animals
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Blood Platelets
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Bone Marrow
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Bone Marrow Cells
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Bone Marrow Transplantation
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Humans
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Megakaryocytes/metabolism*
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Mice
8.Autologous Bone Marrow Derived Stem Cells for the Treatment of Multiple Sclerosis.
Nassim Abi CHAHINE ; Tarek WEHBE ; Johny RASHED ; Ramzi HILAL ; Nada ELIAS
International Journal of Stem Cells 2016;9(2):207-212
Stem cell therapy, an evolving, progressive field of therapeutics has shown several successes in areas where classic treatments failed to prevent or stop disability. Starting in 2009, twenty two sequential patients with progressive Multiple Sclerosis (MS) courses were treated with Autologous Bone Marrow Mononuclear stem cells (BM-MNSCs). The cells were given both intravenously and intrathecally. Using the Expanded Disability Status Scale (EDSS) score for evaluation, our data indicates that the majority of the patients benefited on the average one point on the scale. This paper adds to the body of evidence suggesting the safety and efficacy of autologous BM-MNSCs in the treatment of MS and awaits validation through larger, randomized studies.
Bone Marrow*
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Humans
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Multiple Sclerosis*
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Stem Cells*
10.Fluid shear stress increases the Ca2+ concentration in bone-marrow derived osteoclast-like cells.
Ming CHEN ; Xing LIANG ; Ying WEN ; Bao-jing BAI ; Meng-lu HUANG ; Wei-min GAO
West China Journal of Stomatology 2007;25(4):412-414
OBJECTIVETo study the change of Ca2+ density in cultured osteoclast-like cells in response to fluid shear stress.
METHODSLaser scanning confocal microscope and fluorescent probe were used to detect the free Ca2+ in osteoclast-like cells before and after undergoing fluid shear stress. The images were analyzed and compared with image software.
RESULTSAt 37 degrees C the free Ca2+ in osteoclast-like cells could be labelled effectively with 10 micromol/L Fluo-3/AM. Compared with contol group, the average intensity of Ca2+ fluorescent signal in osteoclast-like cells undergoing fluid shear stress increased significantly.
CONCLUSIONThe Cal2+ concentration in bone-marrow derived osteoclast-like cells is sensitive to fluid shear stress, which suggests osteoclast-like cells modulate their function in response to fluid shear stress through the change of free Ca2+ concentration.
Aniline Compounds ; Bone Marrow ; Bone Marrow Cells ; Calcium ; Cells, Cultured ; Osteoclasts ; Stress, Mechanical ; Xanthenes