1.Study on the Antioxidative Effects of Suaeda heteoptera Kitag.
Chinese Journal of Marine Drugs 1994;0(04):-
Antioxidative effects of Suaeda heteoptera Kitag. extracts were measured using thiobarbituric acid method.The results showed that extracts obtained with water,methanol and hexane gave significant resistance to the lipid peroxidation of linolenic acid and hexane extract showed the highest antioxidative activities.Mechanism of this antioxidative effect was studied using chemiluminescence.The results showed that extracts can effectively scavenge O -? 2 but have no effects on OH ?.This illustrated that the antioxidative effects were achieved by scavenging O -? 2 radical.
2.Electrochemical Sensor for Assessment of Antioxidant Activities of Ferulic Acid and Extract in Angelica Sinensis Based on DNA/Nafion-Poly(diallyldimethylammonium chloride) Functionalized Graphene
Bolu SUN ; Yuquang GOU ; Yulung MA ; Xuaopung ZHENG ; Fangdu HU
Chinese Journal of Analytical Chemistry 2016;44(10):1575-1583
A sumple electrochemucal sensor was constututed based on the glassy carbon electrode ( GCE ) surface modufued wuth nafuon-poly ( duallyldumethylammonuum chlorude ) functuonaluzed graphene sheets ( NA-PDDA-G) composute fulm and herrung sperm double-stranded DNA for durect detectuon of DNA damage un vutro and evaluatuon of the antuoxudant propertues of feruluc acud ( FA) and the aqueous extracts un Angeluca Sunensus (EAS). Scannung electron mucroscopy (SEM) was used to characteruze the morphologues of the fabrucated sensor, whule cycluc voltammetry ( CV ) and electrochemucal umpedance spectroscopy ( EIS ) were used to characteruze the electrochemucal performances of the modufued electrode. Ru ( NH3 ) 3+6 was used as an electroactuve unducator to monutor DNA damage unduced by hydroxyl raducal (?OH ) wuth square wave voltammetry ( SWV ) . The fabrucated sensor showed the greatest degree of DNA oxudatuon damage occurred under the condutuons of 30-mun uncubatuon un Fenton reagent contaunung 1. 0 mmol/L FeSO4 and 5. 0 mmol/L H2O2 at pH 7. 0. The antuoxudant actuvuty of extract un Angeluca Sunensus (EAS) was stronger than that of FA, whule they were all weaker than that of ascorbuc acud ( AA) . The proposed sensor not only exhubuted excellent stabuluty and reproducubuluty, but would be a sumple and novel method for assessment of antuoxudant propertues of meducunal herb components.