Objective: To evaluate effect of Tanreqing injection combined conventional western medicine therapy on acute exacerbation of chronic obstructive pulmonary disease (AECOPD) of Phlegm heat obstructing Lung. Methods: A total of 112 patients with AECOPD were randomly divided into two groups with digital table method from February 2015 to February 2018, 56 cases in each group. The control group was treated byconventional western medicine therapy, and treatment group was treated by Tanreqing injection based on control group. Both groups' treatment lasted for 2 weeks. The modified version of British Medical Reseach Council respiratory questionnaire (mMRC) was used for severity evaluation in breath difficulty. The COPD assessment test (CAT) was used for the assessment of symptoms, activity ability, psychological, social impact and sleep. The FEV1% and FEV1/FVC were recorded. The SaO₂, PaO₂ and PaCO₂ were recorded. The phlegm heat obstructing lung symptom scores were detected. The adverse reactions were recorded. Results: The total effective rate of treatment group was 94.4% (51/54), and the control group was 77.3% (41/53). There was statistically significant difference between two groups (χ²=5.138, P=0.023). After treatment, the mMRC and CAT scores in the treatment group were significantly lower than those in the control group (t value were 17.233, 17.111, respectively, all Ps<0.01); the cough, wheezing, shortness of breath, fever, dry mouth, constipation scores were significantly lower than the control group (t value were 27.717, 29.387, 27.227, 30.268, 28.430, 29.738, respectively, all Ps<0.01). After treatment, the FEV1% (52.34% ± 5.79% vs. 46.98% ± 5.72%, t=4.817), FEV1/FVC (61.36 ± 6.52 vs. 56.93 ± 5.94, t=3.675) in the treatment group were significantly higher than the control group (P<0.01); SaO₂ (90.11% ± 9.53% vs. 83.56% ± 8.84%, t=3.684); the PaO₂ (91.22 ± 9.79 mmHg vs. 85.18 ± 8.80 mmHg, t=3.354) significantly higher than the control group (P<0.01), and PaCO₂ (44.75 ± 4.94 mmHg vs. 51.05 ± 5.46 mmHg, t=6.261) significantly lower than the control group (P<0.01). The incidence of adverse reactions in the treatment group was 11.1% (6/54), and the control group was 7.5% (4/53). There was no statistically significant difference between two groups (χ²=0.091, P=0.763). Conclusions Tanreiqing injection combined with conventional western medicine therapy can take effectsimprove levels of SaO₂ and PaO₂, reduce PaCO₂ level of the AECOPD patients.

Aim To discover specific telomerase inhibitors by high-throughput screening in the more than 2 000 strains of actinomycetes fermentation broth.Methods After verifying the three kinds of screening models for telomerase inhibitor by yeast PCR,the three yeast strains were inoculated overnight into yeast liquid culture which was lacking in histidine and contained 3-AT.Then concentration of yeast strains in culture liquid and dosage of screening sample were regulated for better adjustment to the high-throughput screening.At last the adjusted liquid culture containing yeast strain piped to 96-well plates,drug samples and control article were also added,then the yeast was incubated in the thermostat oscillator.At the same time OD_(595nm) of the yeast liquid was measured every 12 h by using Multifunctional Microplate Analyzer,and the samples whose inhibitory efficiency was over 0.45 were selected for secondary screening.Results The high-throughput screening method related to yeast three-hybrid system was effective in screening samples that had potential telomerase inhibitory ability.The initial concentration of yeast liquid OD_(595nm) was 0.04 and the best dosage of samples was 5 ml.14 active samples,whose inhibitory efficiency was over 0.45,were founded after primary and secondary screening.Conclusions High-throughput screening method related to yeast three-hybrid system is simple and stable in discovering telomerase inhibitors,and 14 new antitumor compounds are identified,which lays the foundation in the development of new anti-tumor agents.

Objective To establish the model of maternal high salt exposure and investigate the effects of maternal high salt exposure on growth and blood pressure in maternal and offspring.Methods At the age of 3 weeks (after weaning) female specific pathogen free (SPF) Sprague Dawley (SD) rats fed for 1 weeks,were randomly divided into three groups,including the high diet salt group (group H,8％ NaCl),low salt diet group (group L,0.26％ NaCl),and normal diet salt group (group N,0.5％ NaCl).Breeding started in feeding until 12 weeks of age,and the inverted microscope was used to see male sperm full view as the zeroth day of pregnancy.Pregnant,lactating,postpartum were grouped by the corresponding feed.Rats fed high salt group after weaning rats continued to high salt diet,denoted as HH group;maternal low salt group after weaning rats received continued low salt diet,denoted as LL group;normal saline group rats after weaning rats fed with normal feed to salt,denoted as NN group.Each period of maternal and offspring weight were measured with the electronic balance.Maternal and offspring's blood pressure was detected with Softron BP-2010A intelligent non-invasive blood pressure instrument (Japan) in different periods.SPSS19.0 software was used to analyze the data,including One-way ANOVA analysis,SNK,and LSD methods.Results High salt exposure,maternal postpartum 2 weeks,and the weight of H group were higher than that of L group,there were no difference in other different periods of maternal.In the offspring,in addition to 32 weeks of age,weight of HH group was lower than that of LL group;and at the age of 8 weeks and 24 weeks,HH group rats body weight was less than the NN group,the differences were statistically significant (P ＜ 0.05).After high salt exposure,after 3 weeks postpartum maternal blood pressure in H group was the highest (158.79 ± 1.87)mmHg.In the offspring,the blood pressure of 60 weeks in the HH group was the highest (162.50 ±2.11) mmHg.At the age of 8 weeks,9 weeks,10 weeks,11 weeks,7 days gestation,pregnant 14 days,1 weeks postpartum,2 weeks postpartum,3 weeks postpartum of maternal,and high salt group's blood pressure were higher than those in normal group,at the age of 9 weeks,10 weeks,11 weeks,7 days of pregnancy,postpartum 1 weeks,2 weeks postpartum,postpartum 3 weeks of high salt group's blood pressure was higher than the low salt group.In the offspring each period of HH group,the blood pressure was higher than that of NN group and LL group,and in the 28,32,36,44,48,and 52 weeks,blood pressure of LL was higher than that of NN group,the differences were statistically significant (P ＜ 0.05).Conclusions Maternal high salt exposure can affect the growth and development of offspring and lead to the occurrence of maternal and offspring hypertension,and with the high salt exposure time,blood pressure was gradually rising trend.