1.Partial Sequence Analysis of Puumala Virus M Segment from Bats in Korea.
Bok Young YUN ; Jeong Joong YOON ; Yun Tae LEE
Journal of the Korean Society of Virology 1999;29(1):23-31
Hantavirus is a genus of the Bunyaviridae family causing two serious diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus is a member of hantavirus originally found in Europe, and its natural reservoir is Clethrionomys glareolus. It is also associated with the hurnan disease nephropathia epidemica, a milder form of HFRS. To identify the hantaviruses in bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area in Korea, and nested RT-PCR was performed with serotype specific primer from M segment. Interestingly, Puumala virus was detected in bats (Rhinolophus ferrum-equinum) only from Won-Joo. The 327 bp nested RT-PCR product, was sequenced. The sequence database search indicates that the sequence is homologous to the published sequence of Puumala viruses. The sequence similarities were ranged from 71% to 97%. The highest sequence similarity was 97% with Puumala virus Vranicam strain, and the lowest was 71% with Puumala virus K27 isolate. Puumala virus Vranicam strain was isolated from a bank vole (Clethrionomys glareolus) in Bosnia-Hercegovina. Puumala virus K27 was isolated from human in Russia. This analysis confirms that bats (Rhinolophus ferrum-equinum) in Korea are natural reservoir of Puumala virus.
Arvicolinae
;
Base Sequence
;
Bunyaviridae
;
Chiroptera*
;
Chungcheongbuk-do
;
Europe
;
Hantavirus
;
Hantavirus Pulmonary Syndrome
;
Hemorrhagic Fever with Renal Syndrome
;
Humans
;
Korea*
;
Puumala virus*
;
Russia
;
Sequence Analysis*
3.A case of prenatal ultrasonographic diagnosis of Roberts syndrome.
Yong Hoon CHO ; Young Don YOON ; Tae Bok SONG ; Ji Soo BYUN ; Young Youn CHOI
Korean Journal of Perinatology 1992;3(1):88-94
No abstract available.
Diagnosis*
4.Effects of sodium vanadate on contractility of vascular smooth muscle.
Young Kwang YOON ; Chang Hyun MOON ; Young Ho LEE ; Bok Soon KANG ; Doo Hee KANG
Korean Circulation Journal 1993;23(1):104-116
The present study was intended to examine the effect of sodium vanadate on contractility of vascular smooth muscle. Aortic ring preparations were made from the rabbit thoracic aorta and endothelial cells were removed from the ring. The contractility of the aortic ring was measured under various conditions. The results were summarized as follows; 1) Sodium vanadate induced contraction of vascular smooth muscle in a dose-dependent fashion. 2) The contractile effects were not blocked by treatments with adrenergic blocking agent(phentolamine) and indomethacin, indicating the direct action of the drug on vascular smooth muscle. 3) In the presence of ouabain, Na(+)-K(+)-ATPase inhibitor, sodium vanadate still increased the contractility of vascular smooth muscle. 4) Treatment with 4.4'-diisothiocyanostilbene-2.2'-disulfonic acid(DIDS) blocked completely the contractile effects of sodium vanadate. 5) In the presence of verapamil, lanthanum and ryanodine, the contractility of the vascular smooth muscle by sodium vanadate was decreased. From the above results. it was suggested that sodium vanadate acts directly on vascular smooth muscle and causes contraction. It was probably due to inhibition of Ca(++)-ATPase in plasma membrane as well as increasing the release of Ca(++) from sarcoplasmic reticulum and Ca(++) influx across the plasma membrane, but not inhibition of Na(+)-K(+)-ATPase.
Aorta, Thoracic
;
Cell Membrane
;
Endothelial Cells
;
Indomethacin
;
Lanthanum
;
Muscle, Smooth, Vascular*
;
Ouabain
;
Ryanodine
;
Sarcoplasmic Reticulum
;
Sodium*
;
Vanadates*
;
Verapamil
5.Perinatal Prognosis of Single Umbilical Artery.
Yoon Ha KIM ; Tae Bok SONG ; Ji Soo BYUN ; Young Youn CHOI ; Ji Young LEE
Korean Journal of Perinatology 1999;10(2):155-160
OBJECTIVE: To evaluate the perinatal prognosis of fetuses with a single umbilical artery. METHODS: From 1992 to 1998, nineteen cases with single umbilical artery(SUA) was observed in 8,704 deliveries at Chonnam University Hospital. RESULTS: Out of nineteen fetuses, thirteen fetuses with single umbilical artery were detected by antenatal ultrasonographic examination and six fetuses were detected after birth. The male to female ratio was 0.9: 1. Congenital malformations were observed in 8 babies(42.1%) and included leg deformity, esophageal atresia, imperforated anus, ventriculomegaly, meningocele, hydronephrosis, ventricular septal defect, joint contracture, cleft lip and palate, toe anomaly, imperforated anus, kyphosis, no urethra and testis, clubfoot, patent ductus arteriosus and rnild mitral regurgitation. Among 10 cases of karyotyping analysis three cases were diagnosed as trisomy 18. Fourteen fetuses(77.8%) showed growth restriction at delivery. Antenatal obstetric complications were hydramnios(n = 3), oligohydramnios(n =2), and severe preeclampsia(n = 3). CONCLUSION: Careful ultrasonographic evaluation for the identification of a SUA is necessary because of its frequent association with congenital anomaly, growth restriction and cytogenetic abnormality.
Anal Canal
;
Chromosome Aberrations
;
Cleft Lip
;
Clubfoot
;
Congenital Abnormalities
;
Contracture
;
Ductus Arteriosus, Patent
;
Esophageal Atresia
;
Female
;
Fetus
;
Heart Septal Defects, Ventricular
;
Humans
;
Hydronephrosis
;
Jeollanam-do
;
Joints
;
Karyotyping
;
Kyphosis
;
Leg
;
Male
;
Meningocele
;
Mitral Valve Insufficiency
;
Palate
;
Parturition
;
Prognosis*
;
Single Umbilical Artery*
;
Testis
;
Toes
;
Trisomy
;
Urethra
6.A comparison of ritodrine and magnesium sulfate for the suppression of preterm labor.
Tae Bok SONG ; Young Don YOON ; Sang Yook LEE ; Seung Kuk KIM
Korean Journal of Perinatology 1992;3(1):33-41
No abstract available.
Female
;
Magnesium Sulfate*
;
Magnesium*
;
Obstetric Labor, Premature*
;
Pregnancy
;
Ritodrine*
7.A case of pregnancy complicated by ruptured endometrioma.
Young Don YOON ; Hye Won CHUNG ; Tae Bok SONG ; Sang Woo JUHNG ; Ji Soo BYUN
Korean Journal of Perinatology 1993;4(3):428-432
No abstract available.
Endometriosis*
;
Female
;
Pregnancy*
8.Protein/creatinine ratio in random urine specimens for quantitation of proteinuria in preeclampsia.
Kyeong Seok JANG ; Sang Yook LEE ; Young Don YOON ; Tae Bok SONG ; Ji Soo BYUN
Korean Journal of Obstetrics and Gynecology 1993;36(7):2147-2151
No abstract available.
Pre-Eclampsia*
;
Proteinuria*
9.A clinical study on the delivery after previous cesarean section.
Hyun Ju KIM ; Young Don YOON ; Tae Bok SONG ; Ji Soo BYUN
Korean Journal of Obstetrics and Gynecology 1993;36(7):2140-2146
No abstract available.
Cesarean Section*
;
Female
;
Pregnancy
10.A case of monozygotic twin with Down syndrome.
Seong Heon JEON ; Choong Hyun YOON ; Young Wook KIM ; Ki Bok KIM
Journal of the Korean Pediatric Society 1993;36(3):434-438
No abstract available.
Down Syndrome*
;
Humans
;
Twins, Monozygotic*