1.Reform of and Practice in Class Teaching Quality Improvement
Jinsong XU ; Shengjuan SHEN ; Bojun DAI
Chinese Journal of Medical Education Research 2005;0(05):-
To improve class teaching quality,we must concentrate on the leading factor: teacher,the key factor: design,and the subjective factor: students.
2.Maxillary sinus carcinoma combined with maxillary sinus fungal sinusitis: one case report.
Zhenxing PENG ; Xianfa XU ; Bojun WEI
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2016;30(2):155-156
A 70 years old male patient complained a sense of swelling on right facial, mild pain, and the obstruction of right nasal, no complain of facial numbness and toothache. Physical examination showed the slight bulging on right facial with mild tenderness, purulent nasal secretions on the right middle nasal meatus, and no significant neoplasm. CT scan showed that soft density tissue in the right maxillary sinus,and the high density tissue in some period. Postoperative diagnosis: carcinoma of maxillary sinus with fungal sinusitis.
Aged
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Carcinoma
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complications
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diagnosis
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Humans
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Male
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Maxillary Sinus
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microbiology
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pathology
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Mycoses
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complications
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Nose Neoplasms
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complications
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diagnosis
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Sinusitis
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microbiology
3.A trial of competition-oriented test methods in histology and embryologytest
Juan XU ; Kaimeng HU ; Minjuan WU ; Ling YANG ; Bojun DAI
Chinese Journal of Medical Education Research 2011;10(10):1213-1215
The examination of Histology and Embryology was reformed by trying competition-oriented test based on an exam system which is supported by attitude assessment and achievement in learning process.The students welcome the system for its advantages such as the original and various forms,its inspiration of enthusiasm and innovation and its humanism.The system contained processional assessment and final assessment as well.Yet the practiser should increase the rate of written examination to keep the fairness.
4.THE TLC UV SCANNING ASSAY FOR TANSHINONE IIA AND CRYPTOTANSHINONE IN YANGXUEZHITONG PILLS
Bojun XU ; Zhiqian DU ; Xingya DU ; Geiwang XU ; Xiufang WANG ; Xueling JIN ;
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(01):-
The TLC UV scanning assay for tanshinone IIA and cryptotanshinone in Yangxuezhitong pills was built up.Their wave lengths were 260 nm and 255 nm respectively.The regressive equation and coefficient of corelation were Y=40.48+5526.90X,r=0.9995 and Y=-12.91+5640.48X,r=0.9999 respectively.The recovery ratio of cryptotanshinone was(?)=102.62%.This me- thod is simple and convenient,and elicits accurate and reproduceable results.
5.Genome-wide identification of SUN gene family in Fragaria vesca and stresses-response analysis.
Yao YU ; Ziyao WANG ; Yiling XU ; Bojun MA ; Xifeng CHEN
Chinese Journal of Biotechnology 2023;39(2):724-740
SUN gene is a group of key genes regulating plant growth and development. Here, SUN gene families of strawberry were identified from the genome of the diploid Fragaria vesca, and their physicochemical properties, genes structure, evolution and genes expression were also analyzed. Our results showed that there were thirty-one FvSUN genes in F. vesca and the FvSUNs encoded proteins were classified into seven groups, and the members in the same group showed high similarity in gene structures and conservative motifs. The electronic subcellular localization of FvSUNs was mainly in the nucleus. Collinearity analysis showed that the members of FvSUN gene family were mainly expanded by segmental duplication in F. vesca, and Arabidopsis and F. vesca shared twenty-three pairs of orthologous SUN genes. According to the expression pattern in different tissues shown by the transcriptome data of F. vesca, the FvSUNs gene can be divided into three types: (1) expressed in nearly all tissues, (2) hardly expressed in any tissues, and (3) expressed in special tissues. The gene expression pattern of FvSUNs was further verified by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, the seedlings of F. vesca were treated by different abiotic stresses, and the expression level of 31 FvSUNs genes were assayed by qRT-PCR. The expression of most of the tested genes was induced by cold, high salt or drought stress. Our studies may facilitate revealing the biological function and molecular mechanism of SUN genes in strawberry.
Fragaria/metabolism*
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Genes, Plant
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Stress, Physiological/genetics*
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Arabidopsis/genetics*
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Plant Development
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Gene Expression Regulation, Plant
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Plant Proteins/metabolism*
6.Biological function and molecular mechanism of the transcription factor GLKs in plants: a review.
Shurong SHEN ; Junjie YUAN ; Yiling XU ; Bojun MA ; Xifeng CHEN
Chinese Journal of Biotechnology 2022;38(8):2700-2712
GLKs (GOLDEN 2-LIKEs) are a group of plant-specific transcription factors regulating the chloroplast biogenesis, differentiation and function maintains by triggering the expression of the photosynthesis-associated nuclear genes (PhANGs). The GLKs also play important roles in nutrient's accumulation in fruits, leaf senescence, immunity and abiotic stress response. The expression of GLK genes were affected by multiple hormones or environmental factors. Therefore, GLKs were considered as the key nodes of regulatory network in plant cells, and potential candidates to improve the photosynthetic capacity of crops. Since numerous researches of GLKs have been reported in plants, the biological function, molecular mechanism of GLKs genes and its applications in breeding were summarized and a GLK-mediated signaling network model was developed. This review may facilitate future research and application of GLKs.
Chloroplasts/genetics*
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Gene Expression Regulation, Plant
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Photosynthesis/genetics*
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Plant Breeding
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Transcription Factors/metabolism*
7.Identification and expression analysis of the YABBY gene family in strawberry.
Tingting YU ; Shurong SHEN ; Yiling XU ; Xinyu WANG ; Yao YU ; Bojun MA ; Xifeng CHEN
Chinese Journal of Biotechnology 2024;40(1):104-121
YABBY proteins are important transcription factors that regulate morphogenesis and organ development in plants. In order to study the YABBY of strawberry, bioinformatic technique were used to identify the YABBY gene families in Fragaria vesca (diploid) and Fragaria×ananassa (octoploid), and then analyze the sequence characters, phylogeny and collinearity of the family members. The RNA-seq data and the quantitative reverse transcription-polymerase chain reaction (qRT-PCR) technique were used to assay the expression patterns of the family members. A green fluorescent protein (GFP) was fused with FvYABBYs and transiently expressed in tobacco leaf cells for the subcellular localization. As the results, six FvYABBY genes and 26 FxaYABBY genes were identified from F. vesca and F.×ananassa, respectively. The FvYABBY genes were grouped into five clades, and five family members were orthologous with AtYABBY genes of Arabidopsis. In F. vesca, all of the FvYABBYs were basically not expressed not expressed in root and receptacle, while FvYABBY1, FvYABBY2, FvYABBY5 and FvYABBY6 were highly expressed in leaf, shoot, flower and achene. In F.×ananassa, FxaYABBY1, FxaYABBY2, FxaYABBY5 and FxaYABBY6 were expressed in achene, and all FxaYABBY were poorly or not expressed in receptacle. Additionally, under the abiotic stresses of low temperature, high salt and drought, the expression of FvYABBY1, FvYABBY3, FvYABBY4 and FvYABBY6 were down-regulated, FvYABBY5 was up-regulated, and FvYABBY2 was up-regulated and then down-regulated. In tobacco leaf cells, the subcellular localization of FvYABBY proteins were in the nucleus. These results provides a foundation for the functional researches of YABBY gene in strawberry.
Fragaria/genetics*
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Arabidopsis
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Biological Assay
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Cold Temperature
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Computational Biology
8.Neurofeedback technology based on functional near infrared spectroscopy imaging and its applications.
Mengqi LI ; Anmin GONG ; Wenya NAN ; Bojun XU ; Peng DING ; Yunfa FU
Journal of Biomedical Engineering 2022;39(5):1041-1049
Neurofeedback (NF) technology based on electroencephalogram (EEG) data or functional magnetic resonance imaging (fMRI) has been widely studied and applied. In contrast, functional near infrared spectroscopy (fNIRS) has become a new technique in NF research in recent years. fNIRS is a neuroimaging technology based on hemodynamics, which has the advantages of low cost, good portability and high spatial resolution, and is more suitable for use in natural environments. At present, there is a lack of comprehensive review on fNIRS-NF technology (fNIRS-NF) in China. In order to provide a reference for the research of fNIRS-NF technology, this paper first describes the principle, key technologies and applications of fNIRS-NF, and focuses on the application of fNIRS-NF. Finally, the future development trend of fNIRS-NF is prospected and summarized. In conclusion, this paper summarizes fNIRS-NF technology and its application, and concludes that fNIRS-NF technology has potential practicability in neurological diseases and related fields. fNIRS can be used as a good method for NF training. This paper is expected to provide reference information for the development of fNIRS-NF technology.
Neurofeedback/methods*
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Spectroscopy, Near-Infrared/methods*
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Brain/diagnostic imaging*
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Magnetic Resonance Imaging
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Technology