AIM: To obtain high level expression of recombinant human truncated osteoprotegerin (TOPG) with higher bioactivity in CHO-DHFR~-cells. METHODS: The recombinant vector pcDNA3.1/DHFR-TOPG was constructed and transfected into CHO-DHFR~- Cells by the directions of LipofectAMINE~(TM)2000 for stable expression. The stable expression cell strains were screened by selective medium IMDM with 50 mL/L FCS, then serially passed in methotraxate (MTX) for gene amplification. The expression were analyzed by ELISA and RT-PCR. At last, the bioactivity analysis was performed in vitro. RESULTS: The expression level of recombinant truncated human OPG was up to 6 mg/L·72 h, and it had significant suppression effect on the formation of OLC(P<0.05). CONCLUSION: Recombinant truncated human OPG has high expression and bioactivity. The results make it possible for further studying and clinical implying of OPG.

Kimura's disease is an important category of reactive lymphadenopathy especially among Asians. It is a chronic inflammatory disorder of unknown etiology and is characterized by angiolymphoid proliferation and eosinophilia. The patient was a 17-year-old man with a 3-year-history of submandibular lymphadenopathy Who was referred to Kyung Hee University Hospital for evaluation of enlarging mass. He had a history of nephrotic syndrome for 7-8 years since 1984. Laboratory findings were not specific except for peripheral blood eosinophilia. The submandibular lymph node showed florid germinal enters, increased postcapillary venules in the paracortex, eosinophilic infiltration, and sclerosis. In this paper, a case of lymphadenopathy of Kimura's disease associated with nephrotic syndrome is reported.
Male ; Humans