Objective To investigate the contamination status of food-borne pathogens in foods sold in Guangzhou, and find the potential food safety hazards, and to provide a scientific basis for formulating food safety measures. Methods From 2013 to 2018, a total of 5 021 food samples from 15 common diets of residents were collected for monitoring Vibrio parahaemolyticus, Staphylococcus aureus, Diarrheagenic Escherichia coli, Vibrio vulnificus, Salmonella, Bacillus cereus, Listeria monocytogenes and Vibrio cholerae. Results Among 5 021 samples, a total of 424 samples were found to carry foodborne pathogens, with a total detection rate of 8.44%. Vibrio parahaemolyticus had the highest detection rate among food-borne pathogens (11.20%), followed by Vibrio vulnificus (9.20%), Bacillus cereus (7.26%), and Staphylococcus aureus (1.72 %), Listeria monocytogenes (1.08%), Escherichia coli (0.86%), and Salmonella (0.48%). Vibrio cholerae was not detected. Among different food categories, the detection rate of aquatic products was the highest, reaching 43.52%, followed by raw aquatic animal food products (16.24%), imported raw livestock and poultry meat (11.11%), foods sold in vendors (10.62%), and baked foods (10.56%). The difference in the detection rate of food-borne pathogens among different foods was statistically significant. Analysis of the detection rate of foods from different sampling locations showed that the highest detection rate was in online shops (22.92%), followed by caterings (12.49%), vendors (11.21%), supermarket (8.995), farmers’ markets (8.59%), and the lowest was retail stores (5.63%). The highest detection rate was found in the third quarter (10.04%), followed by the second quarter (9.81%), and the lowest was in the first quarter (6.43%). Conclusion There was contamination with food-borne pathogens at different degrees in foods sold in Guanghzou. The food safety risk monitoring should be carried out continuously. The prevention and control should be focused on foods with a higher risk of contamination of food-borne pathogens including aquatic animal products, frozen livestock and poultry meat, foods sold in vendors and baked foods.

BACKGROUND: This study aimed to develop a comprehensive instrument for evaluating and ranking clinical practice guidelines, named Scientific, Transparent and Applicable Rankings tool (STAR), and test its reliability, validity, and usability. METHODS: This study set up a multidisciplinary working group including guideline methodologists, statisticians, journal editors, clinicians, and other experts. Scoping review, Delphi methods, and hierarchical analysis were used to develop the STAR tool. We evaluated the instrument's intrinsic and interrater reliability, content and criterion validity, and usability. RESULTS: STAR contained 39 items grouped into 11 domains. The mean intrinsic reliability of the domains, indicated by Cronbach's α coefficient, was 0.588 (95% confidence interval [CI]: 0.414, 0.762). Interrater reliability as assessed with Cohen's kappa coefficient was 0.774 (95% CI: 0.740, 0.807) for methodological evaluators and 0.618 (95% CI: 0.587, 0.648) for clinical evaluators. The overall content validity index was 0.905. Pearson's r correlation for criterion validity was 0.885 (95% CI: 0.804, 0.932). The mean usability score of the items was 4.6 and the median time spent to evaluate each guideline was 20 min. CONCLUSION The instrument performed well in terms of reliability, validity, and efficiency, and can be used for comprehensively evaluating and ranking guidelines.
Reproducibility of Results ; Surveys and Questionnaires ; Practice Guidelines as Topic ; Humans