1. The effect of complement C3a receptor antagonist in the kidney immune injury in trichloroethylene-sensitized mice
Peng YANG ; Dandan ZANG ; Xiaodong YANG ; Bodong LI ; Xian WANG ; Jiaxiang ZHANG ; Qixing ZHU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(3):161-165
Objective:
To explore the effect of complement C3 a-C3a receptor in the kidney immune inju-ry in trichloroethylene-sensitized mice by using C3a receptor specific antagonist C3aRA and discuss the patho-genesis of kidney injury in occupational dermatitis medicamentosa-like of trichloroethylene (ODMLT) .
Methods:
42 female 6~8 weeks old BALB/c mice of specific pathogen free were randomly divided into blank control group (5) , solvent control group (5) , TCE treatment group (16) and TCE+C3aRA treatment group (16) . The TCE treat-ment group and TCE+C3aRA treatment group were further divided into the sensitized group and the non-sensi-tized group according to the skin sensitization test score. Renal function was detected by biochemical detection kit; expression of C3aR in kidney tissue was detected by qPCR; expression of IL-1β and TNF-α protein were de-tected by immunohistochemical.
Results:
Compared with solvent control group and corresponding non-sensitized group, CRE and BUN in TCE sensitized group and TCE + C3aRA sensitized group were significantly increased (
2. Role of complement regulatory protein CD55 in the liver immune injury of trichloroethylene-sensitized mice
Xian WANG ; Cheng ZHANG ; Xiaodong YANG ; Bodong LI ; Dandan ZANG ; Peng YANG ; Jiaxiang ZHANG ; Qixing ZHU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(4):246-250
Objective:
To explore the expression of CD55 in liver tissue of trichloroethylene-sensitized mice and discuss the role of CD55 in the liver immune injury of trichloroethylene-sensitized mice.
Methods:
6-8 weeks specific pathogen free female BALB/c were randomly divided into blank control group, solvent control group and TCE treatment group to establish BALB/c mice sensitized model. According to mouse skin sensitization reaction score, TCE treatment mice were divided into sensitized and non-sensitized group at 24 h after the last challenge. At 48 h after the last challenge, the blood and aseptic livers were collected. The level of serum ALT was tested by automatic biochemical analyzer and pathology of the liver was observed. C5b-9 deposition was studied by immunohistochemistry (IHC) . CD55 protein expression level in liver tissue was studied by immunohistochemistry and Western blotting. The expression of CD55 mRNA in liver tissue was detected by qRT-PCR.
Results:
Liver function test result showed level of serum ALT in TCE sensitized group was significantly higher than solvent control group and TCE non-sensitized group (
3. Effect of cathepsin L activation on trichloroethylene-induced skin allergy in mice
Xiaodong YANG ; Xian WANG ; Bodong LI ; Yun YU ; Qixing ZHU
China Occupational Medicine 2018;45(01):12-18
OBJECTIVE: To observe the expression of cathepsin L( CTSL) on the skin of trichloroethylene( TCE)-sensitized mice,and explore the mechanism of CTSL in TCE-induced immunological skin damage. METHODS: The specific pathogen free female BALB/c mice were randomly divided into blank control group( n = 5),solvent control group( n = 5),TCE group( n = 15) and inhibitor group( n = 15). Skin sensitization experiments were performed according to the maximum guinea pig test method. The TCE group and inhibitor group were divided into sensitized subgroups and non-sensitized subgroups according to skin sensitization results. The skin tissues were taken 72 hours after the last TCE challenge.Hematoxylin-eosin staining was used to observe the pathological structure of skin tissues and measured the thickness of epidermis. The level of Ctsl mRNA was examined by real-time quantitative polymerase chain reaction,and the expression of CTSL, interleukin( IL)-6 and IL-17 were studied by immunohistochemical staining technique. RESULTS: The sensitization rate of TCE group and inhibitor group were 40. 0%(6/15) and 33. 3%(5/15) respectively. There was no statistical difference in the sensitization rate between the two groups( P > 0. 05). The thickness of epidermis and relative expression of Ctsl mRNA,CTSL,IL-6 and IL-17 in TCE sensitized subgroup and inhibitor sensitized subgroup were higher than that in blank control group,solvent control group,TCE non-sensitized subgroup and inhibitor non-sensitized subgroup(P < 0. 05). The above-mentioned indexes were higher in TCE sensitized subgroup than that in inhibitor sensitized subgroup( P < 0. 05). The relative expression of Ctsl mRNA,CTSL,IL-6 and IL-17 in skin of TCE sensitized subgroup were positively correlated between any two indexes( P < 0. 05). CONCLUSION: CTSL activation may play an important role in immunological skin damage of TCE-sensitized mice,which may be related to the promotion of IL-6 and IL-17 release.
4. External physical vibration lithecbole in treatment of ureteral calculi with renal colic used different positions: a prospective multicenter randomized controlled clinical study
Jiacheng ZHANG ; Tianqiang YU ; Zedong LIAO ; Xiangjun LI ; Yanli SUN ; Jun CHEN ; Jun FU ; Bodong LYU ; Yue DUAN
Chinese Journal of Urology 2020;41(1):46-50
Objective:
To evaluate the efficacy and safety of different positions external physical vibration lithecbole (EPVL) therapy for ureteral calculi related renal colic.
Methods:
This study was a prospective multicenter randomized controlled trial. The inclusion criteria was that patients volunteered to participate in the trial and signed informed consent, patients’age ranged from 18-65 years old, ureteral calculi related with renal colic, stone diameter was less than 7 mm, patients were not treated with analgesia, antispasmodic drugs. The exclusion criteria was that combination of severe urinary tract infection, severe hydronephrosis, urinary malformation, severe hypertension, history of cerebrovascular disease, vital organ dysfunction, obesity (BMI>35 kg/m2), history of ureteral calculi exceeded 2 months, abnormal blood coagulation. Patients were randomized into observation group and control group using random number table method. The observation group and the control group were placed on the physical vibration stone arranging machine with head low foot high position and head high foot low position respectively. The inclination angle was 24°. The secondary vibrator vibrated for 6 minutes, then the patient took the prone position and opened the main, the secondary vibrator. The treatment is completed after 6 minutes of vibration. The analgesic effect, stone removal, follow-up effects and adverse reactions in the two groups was compared. We defined the pain relief rate as(VAS score before treatment-VAS score after treatment)/VAS score before treatment×100%.
Results:
A total of 100 patients were included in the study, 50 in the observation group and 50 in the control group. There were no statistical difference in the age of the two groups [(41.8±11.7)years and (46.6±13.9 years)], gender distribution [37(male)/13(female) and 42(male)/ 8(female)], location of stones (in the observation group, 19 cases in upper ureter, 7 cases in the middle ureter and 24 cases in the lower ureter; in the control group, 12 cases in the upper ureter, 3 cases in the middle ureter, and 35 in the lower ureter), left and right distribution of stones [21(right)/ 29 (left) and 22 (right)/ 28(left)], long diameter of stones [(5.2±0.9)mm and(5.1±1.1)mm], VAS scores before treatment (7.5±1.4 and 7.6±1.5), and readmission rate [22%(11/50)With 18%(9/50)], 1 week stone removal rate [70%(35/50) and 64%(32/50)]. The incidence of adverse reactions was 8%(4/50) in the observation group including 3 cases of nausea, 1 case of vomiting. The incidence of adverse reactions was 4% in the control group (2/50), which 2 cases showed nausea. The number of patients who chose EPVL, ESWL or surgery for the subsequent treatment in observation group was 35 cases, 9 cases, and 6 cases respectively. The number of patients who chose EPVL, ESWL or surgery for the subsequent treatment in the control group was 35 cases, 10 cases and 5 cases respectively. There was no significant difference between the two groups (