Medicine, Chinese Traditional

Medicine, Chinese Traditional

Patients who have received coronary artery interventional therapy exhibit visible anxiety and depression, which is closely related to prognosis and life quality. Therefore, for coronary artery stent patients,exception of anticoagulation and antihyperlipedemic drugs, psychological support, cognition therapy and distorted behavior rectification should be applied. The medication of anti-anxiety or depression is of great importance in ameliorate mental state, relieve mental load, as well as enhance prognosis of patients.

The acurate diagnosis of invasive fungal infection(IFI)is the key point for the treatment and proginosis for IFI patients.Pathological diagnosis is still considered as the gold standard for the IFI diagnosis although difficult.The regular procedures for IFI diagnosis including culture and direct examintion are often low yeilding,the discrimination for contamination and infection is hard.Imaging manifestations are nonspecific but have important suggestive values.The detection for molds antigen content is useful,but its sensitivity and specifity need to be improved.The proper diagnosis for IFI relys on the combination of clinical characteristic,imaging manifestion and serial observation.

Objective To understand the effect of environment variables on anxiety in the soldiers at high altitude.Methods A total of 267 soldiers at high altitude were investigated with the self-rating anxiety scale,the environmental scale and Eysenck personality questionnaire.Results Compared with norm(29.78±0.46),the soldiers at high altitude had higher sore of anxiety(37.22±6.64;t=17.92,P＜0.01).One-way ANOVA and independent.samples t test showed that the anxiety(38.37±5.93,36.65±6.951,36.75±6.88)of military people at high altitude had not evidently difference in different altitude(≥3587.5m,3587.5～2767.9m,≤2767.9m).Parental culture.length of military service(F=3.25,P＜0.05)and neuroticism(R=0.52,P＜0.01)had evidently effect on the depression of soldiers.Conclusion The occurrence of anxiety in the soldiers at high altitude has a higher level.Parental culture.1ength of military service and neuroticism had predictive value for depression.

A large number of epidemiological studies have indicated that periodontal disease and ischemic stroke have a certain correlation, which may be its independent risk factor, but the exact mechanism is not fully understood. This article reviews the epidemiological studies of periodontal disease and ischemic stroke as well as its possible mechanism of action.

OBJECTIVE:To study the compatible stability of doxofylline for injection with cefoperazone sodium/sulbactam sodium for Injection. METHODS:The contents of 2 constituents in the mixed solution stored at (25?1)℃ within 6 hours were determined by dual ultraviolet wavelength spectrophotometry,and the appearance and pH value of the mixture were compared with those before mixing. RESULTS:There was no significant change in the content of doxofylline after mixing,while the contents of cefoperazone sodium and sulbactam sodium showed a slight reduction. No significant change was noted for other indexes. CONCLUSION:Doxofylline injection is compatible with cefoperazone sodium and sulbactam sodium for injection in 0.9% sodium chloride injection at 25℃ within 4 hours' mixing.

BACKGROUND: Construction of lentivirus vector has the advantages of simplicity,it is regard as the most effective and successful method in transgene therapy. OBJECTIVE: To clone the metastasis suppressor gene KiSS-1 from human normal placenta tissue and construct its lentivirus vector. DESIGN,TIME AND SETTING: The open experiment was performed at the Laboratory of Fujian Normal University from September 2006 to December 2007. MATERIAL: The pNL-IRES2-EGFP vector was conservated by the Laboratory of Fujian Normal University. METHODS: Total RNA was extracted from human placenta tissue. The opening reading frame cDNA of KiSS-1 was isolated by using RT-PCR,and cloned into its lentiviral vector pNL-IRES2-EGFP to construct expression plasmid pNL-IRES2-EGFP-KiSS-1. MAIN OUTCOME MEASURES: Clone objective gene fragment of KiSS-1,restriction enzyme digestion and gene sequencing of the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 were observed in the study. RESULTS: The nucleotide sequence isolated from the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 was confirmed the same as expected by restriction enzyme digestion and gene sequencing. CONCLUSION: The recombinant plasmid pNL-IRES2-EGFP-KiSS-1 has been constructed successfully.

dbcAMP was injected i. p. into four U_(14) ascitic tumor bearing mice on 3,4,5,6 and 7 days after inoculation of tumor cells. The ascites was aspirated 0.5-1 hour after last injection. For scanning electron microscopy, the tumor cells, washed twice in Hanks solution, were fixed in glutaraldehyde and OsO_4, dehydrated with ethyl alcohol and dried with camphene. For flow microcytometric analysis, the treated tumor cells were fixed in 70% cold ethyl alcohol and stained with propidium iodide. Under SEM, the untreated tumor cells were large, spherical, and uniform in size with numerous long thin microvilli on the cell surface. A few barely visible minute protrusions were present on the microvilli and cell membrane. The great majority of treated tumor cells became smaller and variable in size, with shortened microvilli which reduced in number and even disappeared in some area. Many granular protrusions, larger than that of the control, were clearly observed on the cell membrane and microvilli. The result of flow microcytometric analysis showed that the DNA histogram and cell cycle profile from dbcAMP treated cells have no significant difference from the untreated controls, so it is evident that morphologic changes resulted from dbcAMP were not caused by cell cycle alteration. The morphogenesis of microvilli and cell membrane changes in dbcAMP treated cells is not clear. The relation of these configurations to differentiation of malignant tumor cells is discussed.

Murine ARS reticulum sarcoma cells, when incubated with 1.5, 2.0 and 2.5% DMSO in vitro, were induced to differentiate into macrophage like cells. They showed reduction of cellular and nuclear size and lowering of nucleo-cytoplasmic ratio with marked change in nuclear size. The most effective one was 2.5% DMSO which caused marked reduction of nucleolar size. Tumor cells with nucleo-cytoplasmic ratio less than 0.5 and with reduced nucleoli resembled macrophage. 2.5% DMSO induced the increase of non-specific esterase positive cells and the increase of polystyrene phagocytic percentage and phagocytic index. Under EM, 2.5% DMSO treated ARS cells with nucleocytoplasmic ratio less than 0.5 exhibited abundant cytoplasmic organelles, developed Golgi complex and secondary lysosomes. Scanning EM showed that many large cells with numerous dense slender microvilli were observed in the control. Under the action of 2.5% DMSO, large cells were reduced, small cells were increased and microvilli markedly decreased and shortened. These small cells appeared to be differentiated macrophage like cells and were similar to small cells seen under EM and light microscope. In 2.5% DMSO treated groups, the mitotic index of ARS cells were markedly lowered.