1.Effect of aralosides to contraction function and calcium transient of ischemia/reperfusion myocardial cells.
Miao-di ZHANG ; Gui-bo SUN ; Hui-bo XU ; Min WANG ; Xiao-bo SUN
China Journal of Chinese Materia Medica 2015;40(12):2403-2407
To discuss the protective effect of aralosides (AS) on I/R-induced rat myocardial injury. The adult rat ventricular myocyte ischemia model was established through perfusion with sodium lactate perfusate and reperfusion with Ca(2+) -containing Tyrode's solution simulation. The cell contraction and ion concentration synchronization determination system was applied to detect the effect of AS on single I/R cell contraction and Ca2+ transients. According to the findings, AS could increase resting sarcomere length, contraction amplitude, ± dL/dt(max), calcium transient amplitude and speed of post-reperfusion myocardial cells (P < 0.05, P < 0.01), and decrease in time for achieving 90.0% of maximum relaxation, time for achieving peak value, resting calcium ratio, contraction period [Ca2+] i, time for achieving 50.0% of maximum relaxation and attenuation rate of intracellular calcium transient (P < 0.05, P < 0.01). Therefore, it is suggested that AS improved the post-reperfusion cell contraction and injury of calcium homeostasis.
Animals
;
Aralia
;
chemistry
;
Biological Transport
;
drug effects
;
Calcium
;
metabolism
;
Drugs, Chinese Herbal
;
administration & dosage
;
Humans
;
Male
;
Muscle Contraction
;
drug effects
;
Myocardial Ischemia
;
drug therapy
;
metabolism
;
physiopathology
;
surgery
;
Myocardial Reperfusion
;
Myocytes, Cardiac
;
drug effects
;
physiology
;
Rats
;
Rats, Sprague-Dawley
;
Saponins
;
administration & dosage
2.Clinical observation of sinew-regulating and bone-setting manipulation combined with functional exercise to treat rotator cuff injury
Wen-Di ZHANG ; Zhao-Hui CHEN ; Bo WANG ; Meng-Li YAO ; Han XU ; Xiao-Ming FENG
Journal of Acupuncture and Tuina Science 2018;16(5):329-337
Objective:To explore the clinical efficacy of sinew-regulating and bone-setting manipulation combined with functional exercise to treat mild to moderate rotator cuff injury (RCI).Methods:Forty patients with mild to moderate RCI were randomly divided into a treatment group and a control group,with 20 cases in each group.The treatment group was given sinew-regulating and bone-setting manipulation combined with functional exercise,and the control group was given the same manipulation as the treatment group.The therapeutic effect on the functional improvement was assessed after treatment according to pain threshold,shoulder range of motion (ROM) including flexion,abduction,internal rotation and external rotation,and the University of California at Los Angeles (UCLA) shoulder rating score.Results:Compared with the same group before treatment,the pain threshold,shoulder flexion,abduction,internal and external rotations increased after the treatment in the two groups (all P<0.05).The UCLA shoulder rating score increased (both P<0.05).The improvement in the treatment group is significantly better than that in the control group with a statistical significance (P<0.05).The total effective rate was 95.0% in the treatment group,was higher than 65.0% in the control group (P<0.05).Conclusion:The sinew-regulating and bone-setting manipulation combined with functional exercise relieve the pain of patients with mild to moderate RCI,increase the motion of shoulder joints,and improve the quality of life of patients.The curative effect is better than the treatment of simple sinew-regulating and bone-setting manipulation.
3.Effects of bone marrow mesenchymal stem cells transplantation on the neurotrophic factor expression and cell apoptosis in the rats with spina bifida aperta
Jianing MIAO ; Bo LIU ; Di WU ; Guifeng ZHAO ; Hui LI ; Zhengwei YUAN
International Journal of Pediatrics 2017;44(3):210-213,封3
Objective To observe the effects of bone marrow mesenchymal stem cells (BMSC) transplantation on the expression of nerve growth factor(NGF) and brain derived neurotrophic factor(BDNF) in the rat spinal cord with spina bifida,and to investigate the change in cell apoptosis after BMSC transplantation.Methods Spina bifida aperta was induced with a single intragastric injection of all-trans retinoic acid,then the BMSC was microinjected into spina cord of rat embryos on embryo 16 d(E16),BDNF and NGF were tested by immunofluorescence staining,and TUNEL assay were used for investigating cell apoptosis.Results Transplantation of BMSC enhanced the expression of NGF and BDNF,and reduced cell apoptosis in the defective site of spinal cord.Conclusion The transplantation of BMSC may improve the microenvironment of spinal cord and repair the neurological defects by enhancing the expression of neurotrophic factor and reducing the cell apoptosis.
4.Prevalence of hypertension based on three separate visits among primaryand middle school students in Suzhou City
Di HAN ; Mingzhu SHEN ; Bing SHI ; Bo HAI ; Ziyao DING ; Jieyun YIN ; Hui SHEN ; Jia HU
Journal of Preventive Medicine 2022;34(2):109-113
Objective:
To investigate the prevalence of hypertension among primary and middle school students living in Suzhou City, Jiangsu Province, so as to provide insights into comprehensive hypertension control among children and adolescents.
Methods:
Primary and middle school students at ages of 7 to 17 years were recruited for a questionnaire survey in Suzhou City using the stratified cluster random sampling method from September to December, 2020, and the height and body weight were measured. Blood pressure was measured at three separate clinic visits according to the national criteria Reference of Screening for Elevated Blood Pressure among Children and Adolescents Aged 7-18 Years ( WS/T 610-2018 ), and the detection of elevated blood pressure was estimated at three separate visits. In addition, factors affecting elevated blood pressure were identified.
Results:
A total of 3 713 students were enrolled, including 1 924 boys ( 51.82% ) and 1 789 girls ( 48.18% ). The detection of elevated blood pressure was 13.63%, 5.36%, and 3.37% at three separate visits, respectively, and the prevalence of hypertension ( elevated blood pressure at all three visits ) was 3.37%. The detection rates of elevated blood pressure were all higher at three visits ( 16.90%, 8.40%, and 5.26% ) among students at ages of 12 to 17 years than among students at ages of 7 to 11 years ( 9.65%, 1.67%, and 1.07%, P<0.05 ). The detection of elevated blood pressure was significantly higher in boys ( 15.23% ) than in girls (11.91%) at the first visit ( P<0.05 ), while no significant differences were seen at the second or third visit ( P>0.05 ). In addition, higher detection rates of elevated blood pressure were seen in obese ( 27.62%, 11.51%, and 7.06% ) and overweight students ( 17.45%, 6.95%, and 4.85% ) than in students with normal weight ( 9.44%, 3.54%, and 2.15% ) at all three visits, and greater detection rates of elevated blood pressure were found in obese students than in overweight students at the first and second visits ( P<0.017 ).
Conclusions
The prevalence of hypertension was 3.37% based on three separate visits among primary and middle school students in Suzhou City. Measurement of blood pressure at three separate visits within different days is effective to reduce the false positive rate of hypertension and decrease misdiagnosis among children and adolescents.
5.Influencing factors for myopia among primary and secondary schoolstudents in Suzhou
HU Jia ; DING Zi Yao ; HAN Di ; HAI Bo ; YIN Jie Yun ; SHEN Hui
Journal of Preventive Medicine 2021;33(3):241-245
Objective:
To investigate the influencing factors for myopia among primary and secondary school students in Suzhou, so as to provide basis for myopia prevention and control.
Methods:
The students in Grade 4-12 were recruited by stratified cluster random sampling method. Gender, grade, parents' myopia history, outdoor activity time and video display terminal time were collected through the questionnaire of National Surveillance Program of Influencing Factors for Common Diseases and Health in Students. Uncorrected visual acuity and cycloplegic refraction were tested. Multivariate logistic regression analysis was performed to explore myopia-related factors.
Results:
A total of 990 questionnaires were distributed, and 882 valid questionnaires were recovered, with an effective rate of 89.09%. The prevalence rate of myopia was 78.23% ( 690 cases ). Multivariate logistic regression analysis showed that females ( OR=1.703, 95%CI: 1.173-2.474 ) , middle school students ( OR:5.597-11.949, 95%CI: 3.573-28.349 ) , both parents'myopia ( OR=2.445, 95%CI: 1.597-3.742 ) , video display terminal time over 3 hours per day ( OR=2.026, 95%CI: 1.235-3.325 ) were risk factors for myopia; outdoor activity time over 2 hours per day ( OR: 0.493-0.510, 95%CI: 0.273-0.943 ) was a protective factor for myopia.
Conclusion
The prevalence of myopia among primary and secondary school students in Suzhou is 78.23%. Gender, grade, parents' myopia history, outdoor activity time and video display terminal time are influencing factors for myopia.
6.Effects of Astaxanthin on the damage of osteoblast induced by H2O2.
Ling-peng PEI ; Fu-hui DONG ; Bo-di HUI
China Journal of Orthopaedics and Traumatology 2008;21(3):187-189
OBJECTIVETo investigate the effect of Astaxanthin on enhancing the function of anti-oxidative damage in osteoblast.
METHODSMC3T3-E1 osteoblasts were randomly divided into five groups, including control group, model group, Astaxanthin group [low-dose (1 x 10(-7) mol/L), middle-dose (1 x 10(-6) mol/L), high-dose (1 x 10(-5) mol/L)], in which the activity of cells, activity of superoxide dismutase (SOD), the content of reactive oxygen species (ROS), lipid oxygen (LPO) and membrane fluidity were tested and compared.
RESULTSCompared with Astaxanthin groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased (P < 0.01). However, the contents of ROS and LPO were significantly raised (P < 0.01).
CONCLUSIONH2O2 can cause oxidative damage of MC3T3-E1 osteoblasts, but Astaxanthin can prevent or decrease its influence.
Animals ; Antioxidants ; chemistry ; pharmacology ; Cell Line ; Hydrogen Peroxide ; metabolism ; Lipid Peroxidation ; drug effects ; Membrane Fluidity ; drug effects ; Mice ; Osteoblasts ; drug effects ; metabolism ; Oxidative Stress ; drug effects ; Reactive Oxygen Species ; metabolism ; Superoxide Dismutase ; metabolism ; Xanthophylls ; chemistry ; pharmacology
7.Influence of canthaxanthin on D-galactose induced osseous changes of rat.
Ling-peng PEI ; Bo-di HUI ; Fu-hui DONG
China Journal of Orthopaedics and Traumatology 2008;21(8):613-616
OBJECTIVETo study the influence of canthaxanthin on D-galactose induced osseous changes of rat.
METHODSForty-five six-week-old Wistar male rats were randomly divided into model group, canthaxanthin group and young control group. In addition, 15 sixteen-month-old Wistar male rats were used as old control group. Model group and canthaxanthin group were given injections of D-galactose for 5 months (20 mg/kg/once per-day) to cause aging of rat. Then routine osseous parameters were tested and compared among the 4 groups.
RESULTSCompared with young control group, the BMD, parameters of structural mechanics and biomechanics, bone calcium, manganese, magnesium and the content of hydroxyproline in the model group decreased significantly (P < 0.01), however, the content of bone phosphorus, the activity of bone and serum ALP increased significantly (P < 0.01). Those changes of the model group were the same as the old control group,but the changes in the canthaxanthin group significantly differed with the model group (P < 0.01).
CONCLUSIONThe high does of D-galactose intake can cause aging and osteoporosis at the same time in rat, but canthaxanthin can prevent and inhibit D-galactose induced osseous changes.
Alkaline Phosphatase ; blood ; Animals ; Biomechanical Phenomena ; Bone Density ; drug effects ; Bone and Bones ; chemistry ; drug effects ; Calcium ; analysis ; Canthaxanthin ; pharmacology ; Galactose ; toxicity ; Male ; Malondialdehyde ; blood ; Rats ; Rats, Wistar ; Superoxide Dismutase ; blood
8.Determination of luteolin and luteolin-7-beta-D-glucoside in Chrysanthemum morfolium Ramat. from different collection time by RP-HPLC.
Bi-bo HU ; Hui-di JIANG ; Jun YANG ; Su ZENG
Journal of Zhejiang University. Medical sciences 2004;33(1):29-32
OBJECTIVETo observe the content variation of luteolin and luteolin-7-beta-D-glucoside in Chrysanthemum morifolium Ramat. (CMR) from different collection time.
METHODSRP-HPLC was used to analyze these two components in CMR collected in 2001 and 2002.
RESULTThe content of luteolin was significantly lower than that of luteolin-7-beta-D-glucoside. Furthermore, the former showed no marked changes during collection, while the latter did not varied markedly in early collection but decreased significantly in later collection.
CONCLUSIONThe content of luteolin-7-beta-D-glucoside reflects the quality of Chrysanthemum morifolium Ramat. more viably than that of luteolin.
Chromatography, High Pressure Liquid ; Chrysanthemum ; chemistry ; Flavonoids ; analysis ; Glucosides ; analysis ; Luteolin
9.A survey on the consumption of edible oil among residents in Tongxiang City
Journal of Preventive Medicine 2015;(1):6-9
Objective To learn the consumption of edible oil among residents in Tongxiang City. Methods 180 households were randomly sampled from 6 resident committees. The consumption of edible oil was obtained by questionnaire investigation,and the levels of edible oil consumption between different oil types,survey spots and districts were compared. Results The per-capita of edible oil consumption was(42. 89 ± 5. 83)g. There was significant difference among the per-capita of six survey spots( p<0. 05 ). Among all the residents,rapeseed oil( 55. 00%)and blended oil( 55. 00%) were the most consumed edible oil. Except lard oil,there was no significant difference among different types of edible oils (p>0. 05). In rural area,the per-capita of oil was(48. 46 ± 8. 90)g,which was higher than(35. 78 ± 4. 27)g in city (p<0. 05). Conclusion The consumption level of edible oil among residents is high in Tongxiang City. It's necessary to strengthen the health education and to guide residents intaking edible oil correctly.
10.Unilateral pedicle screw fixation plus interbody fusion by Quadrant system through spatium intermuscular of multifidus.
Qing-chu LI ; Hui-lin HU ; Hui-bo YAN ; Da-di JIN
Chinese Journal of Surgery 2010;48(17):1317-1320
OBJECTIVETo explore the clinical outcome of unilateral pedicle screw fixation plus single cage interbody fusion through spatium intermuscular of multifidus by Quadrant system.
METHODSFrom April 2008 to April 2009, 47 patients underwent unilateral pedicle screw fixation plus single cage interbody fusion through spatium intermuscular of multifidus. There were 22 males and 25 females with the mean age of 58.2 years (range, 46-74 years). Among them 12 cases had far-lateral lumbar disc herniation, 7 cases had post-discectomy recurrence, and 28 cases had degenerative instability. Thirty-seven cases were treated with lumbar interbody fusion through transforaminal approach, 10 cases through posterior approach. After surgery, the radiography was carried out to demonstrate the fusion status, and the Nakai criterion was used for assessment.
RESULTSThe average skin incision length was 3.2 cm (range, 3.0 to 3.5 cm), the average operative time was 90 min (range, 70 to 160 min), and the average blood loss was 130 ml (range, 90 to 360 ml). All cases were followed up for 8 - 20 months (average 13.6 months). Postoperative radiography showed no evidence of instrument failure, and 43 cases got bone fusion, 4 cases got suspicious fusion. At final followed-up the average leg pain VAS decreased from 7.4 ± 1.1 preoperatively to 2.4 ± 1.3 postoperatively, the average low back pain VAS decreased from 6.7 ± 1.3 preoperatively to 1.8 ± 1.5 postoperatively. According to Nakai criterion, 31 cases were rated as excellent, 11 cases as good, and 5 cases as fair with the total excellent and good rate of 89.4%.
CONCLUSIONSUnilateral pedicle screw fixation plus single cage interbody fusion through spatium intermuscular of multifidus has some advantages of minimal invasiveness, less blood loss, less complications and reliable curative effect. It is a satisfactory lumbar fusion method under suitable indication.
Aged ; Bone Screws ; Female ; Follow-Up Studies ; Humans ; Lumbar Vertebrae ; surgery ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; Muscle, Skeletal ; surgery ; Spinal Fusion ; methods ; Treatment Outcome