Objective:Studies suggested that some growth factors and their receptors play important roles in the treatment and prognosis of lung cancer.In this study we investigated the expression of epidermal growth factor receptor(EGFR),vascular endothelial growth factor(VEGF),and Ki-67 simultaneously in the tissues of aged patients with non-small cell lung cancer(NSCLC). Methods:The expression levels of EGFR,VEGF,and Ki-67 in 64 cases of aged patients with NSCLC were detected by immunohistochemistry staining.Results:The positive expression of EGFR,VEGF and Ki-67 in the tissue of lung cancer was 59.4%,57.8% and 92.2%,respectively.It is different significantly while compared with control group of benign tissues.The expression levels of EGFR and VEGF are correlated significantly with the pathologic type,differential degree,TNM stage and lymphatic metastasis.The expression level of Ki-67 is related to differential degree and TNM stage of lung cancer.Conclusion:The method of immunohistochemistry to detect multiple biological markers simultaneously is simple and feasible.It can supply important information for the staging and prognoses of patients with NSCLC,and more than that,it can also provide the theoretic basis for the individualized molecular targeting therapy of NSCLC.

In this study, the processes of pollination ecology of Fritillaria delavayi were investigated to document its reproductive characteristics. Some individuals of F. delavayi could produce seeds under bagging without emasculation (11%), but the rate was significantly lower than that of the natural control (87%). It is suggesting that pollination of F. delavayi largely depends on pollen vectors. Bombus sushikini was the only effective pollinator of F. delavayi and the visitation frequency was 0.003 time xXflower(-1) x min(-1). Flowering of F. delavayi in whole population lasted for 35 d and single flower for 11 d. Pollen viability and stigma receptivity lasted for 9 d and were relatively long compared with other Fritillaria genus plants. Consequently, bumblebee pollination and long floral longevity seem to be important for reproductive assurance of F. delavayi in harsh alpine environments.
Animals ; Bees ; physiology ; Fritillaria ; physiology ; Pollen ; physiology ; Pollination

Objective To understand the prevalence,drug resistance and clinical features of children with acute lower respiratory tract infection(ALRI) caused by escherichia coli.Methods From Oct. 2005 to Oct. 2006,659 patients with ALRI who were admitted to hospital were chosen and their nasopharyngeal secretions were obtained and cultured.K-B disc diffusion for antibiotic susceptibility were performed for these clinical isolates.Results Among 659 patients,118 cases were isolated escherichia coli,the rate was 17.99% which had 90 boys and 28 girls.Eighty-seven of 118 E.coli strains were with extended-spectrum ?-lactamase(ESBLs),the rate was 73.73%.All of strains were sensitive to imipenem.For ESBLs-producing strains,the ratio of resistance tocefotaxime,ceftriaxone,cefuroxime,ampicillin,piperacillin were 78.81%,73.73%,73.73%,76.27%,78.81%,respectively.Conclusions The positive rate of ESBLs producing E coli in Kunming area is high and drug resistance is severe gradually.Imipenem can be the first selection for treatment on these infections.

Objective : To understand the relationship between sleep duration and low vision among primary and middle school students, so as to provide the reference for promoting eye health of primary and middle school students. Methods : Using stratified cluster sampling method, the students were selected from four primary schools, four junior high schools and three senior high schools in Dinghai, Putuo, Daishan and Shengsi of Zhoushan. The questionnaire for the influencing factors of myopia in the national monitoring and intervention project of common diseases and influencing factors among students in 2019 was used to collect their demography, video display terminal use, outdoor activity, parents'myopia and sleep duration. The multivariate logistic regression model was established to analyze the relationship between sleep duration and low vision among the students. Results : A total of 3 016 questionnaires were sent out, and 2 932 valid ones were returned. The effective rate was 97.21%. There were 1 537 ( 52.42% ) males and 1 395 females ( 47.58% ); 947 ( 32.30% ) primary school students and 1 985 ( 67.70% ) middle school students; 2 328 ( 79.40% ) students with low vision. There were 1 022 ( 34.85% ), 903 (30.80%), 697 ( 23.77% ) and 310 ( 10.57% ) students with the daily sleep duration of <8 hours, 8-<9 hours, 9-<10 hours and ≥10 hours, respectively. The multivariate logistic regression analysis showed that the risk of low vision was lower when the daily sleep duration of students was 8-<9 hours ( OR=0.735, 95%CI: 0.561-0.963 ) and 9-<10 hours ( OR=0.605, 95%CI: 0.446-0.825 ). Conclusion Adequate sleep duration is conducive to reduce the risk of low vision among primary and middle school students.

Adult ; Coronary Angiography ; Drug-Eluting Stents ; adverse effects ; Humans ; Male ; Multidetector Computed Tomography ; Percutaneous Coronary Intervention ; adverse effects ; Sirolimus ; Thrombosis ; diagnosis ; etiology ; Ultrasonography, Interventional

Child ; Child, Preschool ; Female ; Flow Cytometry ; methods ; Humans ; Infant ; Male ; Thrombasthenia ; classification ; diagnosis

Abstract: Objective　To master the condition of cockroach population distribution, seasonal dynamics, cockroach density for different habitat, and to provide a basis for developing cockroach control strategies. Methods　Six types of surveillance sites, including residential areas, hotels, restaurants, supermarkets, hospitals and farm product markets, were set up in 14 cities in Liaoning to monitor cockroaches using the sticky-trap method. The cockroach surveillance data from vector surveillance sites in fourteen cities of Liaoning Province in 2021 were collected and statistically analyzed using Excel 2010 and SPSS 23.0 software. The density and species composition of cockroaches were analyzed, and the density difference and seasonal dynamics trend of cockroaches in different habitats were compared. Results　A total of 3 031 cockroaches were captured in 2021, of which Blattella germanica accounted for 94.66% (2 869/3 031) and was the dominant population. The total density of cockroaches was 0.230 0 cockroaches per sheet (3 031/13 234) and the total infestation rate of cockroaches was 5.59% (562/10 052). The density and infestation rate of cockroaches in different habitats were in the order of farm product markets, restaurants, and hotels and the difference in infestation rate between habitats was statistically significant (χ2=168.327, P<0.05). The seasonal dynamics trend of cockroach density and disoperation rate showed a unimodal curve, and the peaks were distributed in July. The seasonal dynamics of cockroach density and disoperation rate in different habitats showed a unimodal curve in the habitats of farm product markets, supermarkets, hotels, hospitals and residential areas all, while the habitats of restaurants were close to a double peak curve. Conclusions　B. germanica is the dominant species of cockroaches in Liaoning Province in 2021. Compared with 2020 the density and disoperation rate of cockroach in 2021 showed a slight downward trend, and the seasonal dynamics trend of cockroach density and disoperation rate showed a unimodal curve. The farm product markets are the key places for cockroach prevention and control. According to the seasonal fluctuation trends in cockroach density and infestation rate in different habitats, comprehensive prevention and control measures should be taken before the peak periods to reduce cockroach density and control diseases.

Adult ; Aneurysm, Dissecting ; complications ; Coronary Aneurysm ; complications ; Female ; Humans ; Myocardial Bridging ; complications ; Myocardial Infarction ; etiology

Objective:To investigate the gender specific differences clinical characteristics of coronary spasm angina patients by comparing differences of the risk factors ,clinical manifestations and coronary angiography with different gender . Methods:The clinical data of 114 patients with coronary spasm angina admitted to Zhongshan Hospital ,Fudan University from January 2003 to December 2013 were retrospectively analyzed .The patients were divided into two groups according to gender:male group (n= 99) and female group (n= 15) .The differences of the risk factors ,clinical manifestations and coronary angiography between these two groups were compared . Results: The percentage of smoking patients in the male group (75 .8% ) was higher than that in the female group (6 .7% , P< 0 .001) .Similarly ,higher uric acid levels ([350 .6 ± 96 .2]μmol/L vs [249 .1 ± 63 .1] μmol/L , P< 0 .001) and lower high density lipoprotein-cholesterol ([1 .09 ± 0 .29] mmol/L vs [1 .30 ± 0 .35] mmol/L , P= 0 .02) were also found in the male group .No statistically significant differences were found between the two groups in other risk factors ,clinical manifestations or coronary angiography .Conclusions:Male coronary spasm angina patients seemed to more smoke ,have higher uric acid levels ,and lower high density lipoprotein-cholesterol levels than female patients .

OBJECTIVETo investigate the expression of guanylin in colorectal cancer.

METHODSThe expression of guanylin was examined by RT-PCR and semiquantitative analysis in 20 cases of colorectal cancer, and its relationship with clinical characteristics was analyzed.

RESULTSThe positive expression of guanylin in normal tissue (80%, 16/20) was significantly higher than that in tumor tissue (35%, 7/20) (P<0.01). The same result was found in the semiquantitative analysis of 14 cases with differential expression. Differential expression of guanylin in colorectal cancer was associate with TNM stage (P<0.05), not with sex, Borrmann type and degree of differentiation (all P>0.05).

CONCLUSIONThere is differential expression of guanylin in colorectal cancer, and this kind of differential expression is associated with tumor TNM stage.

Colorectal Neoplasms ; metabolism ; pathology ; Female ; Gastrointestinal Hormones ; metabolism ; Humans ; Male ; Middle Aged ; Natriuretic Peptides ; metabolism ; Neoplasm Staging