Objective To evaluate the efficacy and safety of combined intrapleural therapy with bevacizumab and cisplatin in controlling malignant pleural effusion(MPE)caused by non?small cell lung cancer(NSCLC). Methods A total of 63 NSCLC study subjects with MPE were randomly assigned to one of two groups(A and B). Group A(bevacizumab,n=32)received intrapleural bevacizumab(5 mg/kg)with cisplatin(40 mg/m2) therapy once a week for a total of three cycles. Group B(cisplatin,n=31)received intrapleural cisplatin therapy alone with the same dose and cycle. Pleural fluid was collected from both groups prior to and after treatment. The levels of VEGF in the pleural fluid were determined by ELISA. The cura?tive effect and adverse reaction were observed and follow?up was conducted. Results In 63 evaluable study subjects,the curative efficacy in the be?vacizumab group was significantly higher than that found in the cisplatin group(84.3%vs 61.3%,respectively,P<0.05). But the overall survival (OS)of the two groups showed no statistically significant difference(13.0 months vs 12.0 months,P>0.05). After treatment,The VEGF levels in the MPE were significantly decreased in both groups. In the bevacizumab group,the levels of VEGF in the pleural fluid were significantly lower com?pared to those of the cisplatin group after treatment,showing greater efficacy(P<0.05). The VEGF levels of the two groups whose treatment was in?valid had no statistically significant difference(P=0.079). There was no significant difference in gradeⅢ/Ⅳadverse events between the two groups. All procedures were well tolerated by the patients. Conclusion Combined intrapleural therapy with bevacizumab and cisplatin was effective and safe in managing NSCLC?mediated MPE. The patients showed high tolerance to the therapy. For prediction of local treatment efficacy of bevaci?zumab using the VEGF expression levels in MPE,further research was needed.

Objective To study the inhibitory effects of stachyose on the hyperacute rejection in pig-to-human heart xenotransplantation.Methods A pig-to-human xenogeneic heart transplantation model was established based on an in vitro free heart blood perfusion system.The pig hearts were di- vided into two groups:group A(pig hearts treated with human blood perfusion as control)and group B(pig hearts treated with human blood plus stachyose perfusion).After perfusion for 1h,the heart xenografts were examined for deposit of lgG and IgM by immunohistochemistry and pathological analy sis.Results The mean survival time of perfusion hearts in groups A and B was(9.5?2.5)min and (46.8?8.1)min respectively(P

Animals ; Biological Specimen Banks ; utilization ; Humans ; Specimen Handling ; methods ; Tissue Fixation ; methods

Adult ; Elbow Joint ; injuries ; Female ; Humans ; Joint Dislocations ; therapy ; Musculoskeletal Manipulations ; Radius ; abnormalities ; Radius Fractures ; therapy ; Synostosis ; therapy ; Ulna ; abnormalities

BACKGROUND:Immunity of fetal liver stem cel transplantation is rarely reported, syngeneic and al ogeneic fetal liver stem cel transplantation in the treatment of hepatic cirrhosis is stil unclear.
OBJECTIVE:To observe the therapeutic effects of syngeneic and al ogeneic fetal liver stem cel transplantation on hepatic cirrhosis as wel as immune rejections during the therapeutic process.
METHODS:The fetal liver stem/progenitor cel s from BALB/c and C57BL/6 mice were isolated and purified by the type IV col agen enzyme digestion method. A total of 104 healthy BALB/c mice were randomly assigned to four groups. Normal control group:no treatment;Hepatic cirrhosis group, syngeneic transplantation group and al ogeneic transplantation group:16 weeks after hepatic cirrhosis models of mice were developed by intraperitoneal injection with carbon tetrachloride, physiological saline, syngeneic fetal liver stem cel s and al ogeneic fetal liver stem cel s were injected via the caudal vein. Final y, the survival statuses, liver function, hepatic fibrosis index, the number and ratio of immune cel s (CD4+T, CD8+T, NK, NKT) and histopathologic examinations were compared in each group after transplantation 4 weeks.
RESULTS AND CONCLUSION:The survival rates in the two transplantation groups were both 100%, which was significantly higher than that in the hepatic cirrhosis group (67%, P<0.05). The liver function and liver fibrosis index in each group did not show statistical differences (P>0.05). Immunological tests showed no difference between groups (P>0.05). Pathohistology examination of hepatic tissue repair:Al ogeneic transplantation group>syngeneic transplantation group>hepatic cirrhosis group. Hence, fetal liver stem cel transplantation via the caudal vein could elevate the survival rate of hepatic cirrhosis mice, al eviate the degree of hepatocyte necrosis. There is no immunologic rejection during syngeneic and al ogeneic fetal liver stem cel transplantation that could help to treat hepatic cirrhosis in mice.

Objective To establish a stable transplantation tolerance model by combined treatment with PTD-mFoxp3 fusion protein and allogeneic bone marrow transplantation and study its application to reduce the incidence of graft-versus-host disease (GVHD).Method BALB/c mice as recipients were randomly divided into four groups,accepted medical linear accelerator ray 3.0-Gy total body irradiation (TBI) before bone marrow transplantation (BMT),and injected with donor C57BL/6 mice bone marrow cells 3 × 107withinn 4-6 h.The BALB/c mice in group A were given PTD-mFoxp3 fusion protein through tail vein intermittently (day-2,0,1,3,5,7,9,11,13),those in group B given the same dose mFoxp3 protein,those in group C given normal saline,and those in blank control group given no treatment.The symptoms of GVHD were observed after BMT.Chimerisms were determined on the week 1,2,4,8 and12 post-BMT by flow cytometry.Liver and intestinal tissues were collected for pathological examination.Recipient immune response was assessed on the week 4 and 12 by mixed lymphocyte reactions (MLR) after BMT.Results The chimerism level in group A was high [(38.16 ± 3.09) ％] in the first 12 weeks after BMT,and that in group B and group C was reduced [(20.12 ± 4.75) ％ and (15.72 ± 2.36) ％ respectively,P＜0.05].MLR revealed that shown lymphocyte donor-derived lymphocyte proliferation rate at 4th and 12th week in group A was significantly lower than in other groups (P＜0.05).Pathological examination showed infiltration of lymphocytes in the liver and intestine was milder in group A than in other groups.Conclusion PTD-mFoxp3 fusion protein combined with allogeneic bone marrow transplantation can effectively establish a stable transplantation chimeric model and reduce the incidence of GVHD.

BACKGROUND: Patients with amnestic mild cognitive impairment (MCI)were considered to be going to develop into Alzheimer disease, and were the high-risk group of Alzheimer disease.OBJECTIVE: To study the implicit and explicit memory in the patient with amnestic MCI. DESIGN: Homochronous and controlled observation.SETTING: Neurological Department of the Southern Building of General Hospital of Chinese PLA.PARTICIPANTS: The research was carried out at Neurological Department of the Southern Building of General Hospital of Chinese PLA from January to June 2003. Totally 20 male patients with amnestic mild MCI,with the average age of (78.7±4.9) years and the average educational time of (8.2±1.0) years, selected from neurological out-patient clinic and ward of southern building of General Hospital of Chinese PLA, Complaint memory losing and confirmed by the relatives and close friends of the patients, Mini-mental state examination (MMSE) ≥24, activity of daily living (ADL)＜26, and clinical dementia rating equal to 0.5, Objective memory examination score reduced compared to the examiners' educational background,the memory quotient recorded by clinical memory sheet to be 63-77 (lower than the normal value with 1.5-2.5 standard deviation), were accepted, and determined as experiment group.Totally 20 healthy male veterans selected from Beijing area, with the average age of(77.9±4.7) years and the average educational time (8.1±1.1) years were selected as control group. All subjects volunteered to take part in_the examination.METHODS: Explicit memory included word free recall and re-recognition task. The Chinese characters were selected from the 1000 most commonly used ones in the books of primary and middle schools. The 30 double characters-words were chosen and randomly divided into two tasks. Recall task included 10 double character words that were target words. Re-recognition task included 20 double character words, of which one half were target words, and the other half were interfering words. Implicit memory included word free recall and character-root-complementary task. The Chinese characters were from the same source as mentioned before. Thirty Chinese characters were chosen in free recall task at beginning. These Chinese characters at least had possibility of composing over 4 commonly used double character-words in primary and middle school books. One double-Chinese character-word in four possibilities was randomly selected and the total was 30 words. Then these 30 words were divided into two groups, one was target group and the other was interfering word. All selected Chinese characters were composed either up-down structure or right-left structure. Taking a part of the Chinese character would leave a characterroot. There were 30 character-roots and they were never the same each other.There were at least four possibilities to form a Chinese character for each root as properly matching among 1000 commonly used Chinese characters.At first, the subjects were asked to read the target words loudly for 3 times in 4 tasks and tried to remember all the target words (characters) and were told to test their memorization later on. The target words (or characters)would appear in one-minute interval. The subjects were asked to repeat the words (or characters) appeared before as many as possible based on their memory in free recall task and they did not have to remember the words (or characters) in order. The correct recall rate for 10 target words was calculated. In re-recognition task, the target words and interfering words were mixed up and appeared one by one in random order and the subjects were asked to tell if the words appeared before. The correct rate of 20-word rerecognition was calculated. In free recall task, the first character in target words was mixed with the first character in interfering words and they appeared one by one in random order. The subjects were asked to form any two-character-word with appeared Chinese characters and wrote down the first word thought of in test sheet. The hitting rate of target words and interfering words were calculated. t-test for independent samples was carried 9ut respectively between the two groups.MAIN OUTCOME MEASURES: ① Semantic priming effect (the hitting rate of target words in word free recall task and the hitting rate of interfering words) and apperception priming effect (the hitting rate of target words in character-root-complementary task and the hitting rate of interfering words) in the two groups. ② Difference of explicit memory (correct rate of free recall and correct rate of re-recognition) and difference of semantic and apperception priming effect in the two groups.RESULTS: All subjects in the two groups completed the test and entered the final analysis. ① Results of inner-group analysis: In the group of MCI,the hitting rate (0.41±0.15) of target words in word free recall task was significantly higher than that of interfering words (0.31±0.12, P ＜ 0.01),suggesting that there existed semantic priming effect in the patients with amnestic MCI as completing this task. In the character-root-complementary task, the hitting rate of target words (0.17±0.10) was significantly higher than that of the hitting rate of interfering words (0.10±0.07, P ＜ 0.05), suggesting that there existed perceptional priming effect in the patients with amnestic MCI as completing this task. In the control group, the hitting rate of target words (0.45±0.11) was significantly higher than that of the interfering words (0.33±0.10, P ＜ 0.01), suggesting that there existed semantic priming effect in the elderly with normal perception as completing this task as well. In the character-root-complementary task, the hitting rate of target characters (0.18±0.08) was higher than that of interfering characters (0.13±0.07, P ＜ 0.05), suggesting that there existed perceptional priming effect in the elderly with normal perception as completing this task as well. ② Results of inter-groups analysis: Correct rates of free recall and re-recognition in the MCI group was decreased (0.10±0.09, 0.45±0.13,t=9.858, P=0.000; 0.69±0.14, 0.83±0.08, t=3.685, P=0.000). There was not significantly different in Sementic priming effect and perceptional priming effect both in the MCI group and the control group.CONCLUSION: Sementic priming effect and perceptional priming effect were existed both in the MCI experiment group and the control group, but the implicit memory was not significantly different in the two groups. However, the patients with forgetful style MCI were exhibited a decline in explicit memory. Memory function of mild MCI patients was shown double characteristics, i.e. damage of explicit memory and preservation of implicit memory. The double character in memorial function provided theoretical basis for the rehabilitative training of MCI patients.

Objective To investigate the current status of medication compliance of patients with anky-losing spondylifis and analyze its related factors. Methods Seventy-four patients with ankylosing spondylitis were investigated about their general conditions,medication compliance and its related factors.The results were analyzed. Results Thirty-nine patients(52.7%) had good medication compliance while the rest 35 cases has poor medication compliance among which 24 patients with drug withdrawal exceeded 1 month.The medication compliance for patients with disease process of lower than 0.5 year,0.5 to 1.0 year,1 to 2 years and above 2 years was 82.0%,60.0%,25.0%and 31.0%.The logistic regression analysis showed that the disease process,dis-ease knowledge and media advertisement were the main influencing factor for medication compliance,while the age,sex,marital status,education background,adverse reaction,satisfaction degree with medication effect, economic conditions and depression were not major influencing factors. Conclusions About half of patients with anky-losing spondylitis had poor medication compliance,especialy with disease process of above 1 year.The main in-fluencing factors include disease process,disease knowledge and media advertisement.

Objective To investigate the difference between anagenetic fibula with normal fibula in mechanical parameters, osteogenesis and tissue morphology,through establishing the model of fibula defect in rabbit.Methods Twenty New Zealand white rabbits were randomly divided.Model group were cuted the right fibula bone with 1.5 cm length.X-ray was used to observe the anagenetic fibula(half month,one month,two months).Stumped the anagenetic and normal fibula(1.5 cm)after two months. Three-point bending test was used to test the mechanical properties;alkaline phosphatase staining was checked the bone-formation ability;HE staining to check the tissue morphology.Results After half month a few new bone were formed at the edges of resec-tion area,one month later new bone were growed in the donor site,and after two month new fibula were completely formed.The three point bending test of fibula showed:there was no statistically significant difference between anagenetic fibula and normal fibu-la(P >0.05);alkaline phosphatase staining and HE staining showed the anagenetic fibula bone forming ability,histology had no sig-nificant difference compared with normal fibula.Conclusion Fibula were regenerated successfully after removing which retaining the periosteum.Compared with the normal fibula,there were no remarkable differences in mechanical parameters,osteogenesis and tissue morphology.

A new method was developed for the determination of 11 fat_soluble vitamins ( A, D, E and K) and its derivatives in vitamin tablets by ultra performance convergence chromatography ( UPC2 ) . The mobile phase was the mixture of supercritical CO2 and acetonitrile at a flow rate of 1 mL/min. The separation was carried out on the Waters Acquity UPC2 HSS C18 SB 100 mm × 3. 0 mm i. d. , 1. 8 μm column. The UV detector was set at a wavelength of 284 nm. The limits of detection ( LOD) were 1. 5-2. 0 mg/L, and the calibration linear for VK1 , VK2 , VK3 and VB3 was 3-300 mg/L, linear for VA, VA palmitate, VA formic acid, VE, VE acetate, VD2 and VD3 was 5-300 mg/L, respectively. Its spiked recoveries were 97. 31%-98. 76%, and the relative standard deviations ( RSDs) were 0. 41%-0. 96%. The method is applicable for the determination of fat_soluble vitamins ( A, D, E and K) and Their derivatives in vitamin tablets.