Objective To study the impact of gender,gender role and gender traits on depression in adolescents.Methods A sample of 1330 middle school students from seven provinces in China completed Beck Depression Inventory (BDI) and Gender Traits Inventory.Results 27.1 percents of participants displayed the symptoms of depression.The mean score of males of BDI was 10.04 ± 8.38 while that of females was 10.33 ± 8.37,indicating that there was no sex differences in depression of adolescents(P=0.535).The participants could be classified into 4 sex role types:masculine,feminine,androgynous and undifferentiated,and the BDI mean score of each type was 9.51 ± 7.66,12.30 ± 8.81,9.14 ± 8.10,10.72 ± 8.66,and significant differences were found among adolescents with different gender roles (P =0.000).BDI scores of the feminine adolescents were the highest among the four gender roles,which were significantly higher than scores of the masculine and androgynous adolescents (P =0.000).Regression analysis indicated that adolescents with more positive masculine traits tended to be less depressive (t =-4.966,P ＜ 0.01) while adolescents with more negative masculine (t =2.574,P ＜ 0.01) or feminine (t =10.566,P ＜ 0.01)traits tended to be more depressive.Conclusion Gender role rather than gender has greater impacts on adolescent depression,and specifically,positive masculine traits represent protective factors for depression.

Objective:To investigate the efficacy of tramadol hydrochloride tablets in the control of periodontal pain following ortho-dontic treatment.Methods:76 orthodontic patients were randomly assigned to I,II,III and IV groups according to the different doses of tramadol hydrochloride tablets or placebo(control).Periodontal pain was recorded and analysed by VAS,adverse reaction was ob-served.Results:The pain scores were all lower when teeth were not touching than when teeth were touching.Pain scores of experi-mental group were less than that of the control group,muff-doses are more effective than single-dose.No obvious adverse reaction was found in all group.Conclusion:Tramadol hydrochloride tablet is effective in the control of the pain following orthodontic treatment.

Objective To analyze the curative effects and complications of ventriculo-peritoneal shunt in treating hydrocephalus caused by different diseases in children. Methods The data of 289 children with hydrocephalus who were performed ventriculo-peritoneal shunt were examined and the incidence rate of complications was analyzed. Results Thirty-seven incidences of complications occurred in 24 patients with the total incidence rate of 12.46%.There was obstruction of proximate tube in five cases,distal end in two cases,with the obstruction rate of 2.42%.Postoperative infection happened in nine cases(3.11%).Bacterial culture was positive in three cases,two of which were Staphylococcus epidermidis and Bacillus coli in the other one.Negative results were found in six patients for the bacterial culture.Subdural hematoma occured in one case,and tube break after injury in two cases.Slit ventricle syndrome broke out seven times in three cases,leakage of cerebrospinal fluid in two,exposure of pumps in three,and subcutaneous hydrops in two.Four cases(1.38%)were dead from infection. Conclusion Ventriculo-peritoneal shunt is preferred for hydrocephalus in children if the complications are well prevented and controlled.

Objective To investigate human immunodeficiency virus type 1 (HIV 1)specific CD8'T cell responses and related human leukocyte antigenⅠ(HLA-I)restriction sites.Methods Four Epstein-Barr virus (EBV)strains-transformed B-lymphoblastoid cell lines (BLCL)from periph eral blood monocytes (PBMC)of 4 healthy individuals were established.Using synthesized peptides in HIV-1 Gag,Vif and Nef regions as epitopes and using BLCL as antigen presenting cells (APC), the frequency of interferon-7(IFN-?)secreting cells in CD8 T cells from a HIV-infected long term nonprogressor by enzyme-linked immunospot (ELISPOT)assay in vitro were assessed.Moreover, matching analysis of HI.A-Ⅰrestriction sites were performed.Results HIV-1 epitopes were presen ted restrictively by HLA-Ⅰmolecules in specific CD8 T cell responses,and this antigen presenting had high specificity.HLA-Ⅰrestriction sitesA03,A26,C04andC08 contributed to presentepitopes in HIV-1 C-ag region,A03 and C08 to HIV-1 Nef as well.Conclusion HLA-Ⅰrestriction sites may include A03,A26,C04 and C08 in HIV-1 Gag region,and A03,C08 in Nef region.

ABSTRACT:Objective To verify whether rosuvastatin can inhibit homocysteine (Hcy)‐induced rat aortic vascular smooth muscle cell (VSMC ) phenotype transformation and its potential mechanism .Methods The primary culture and identification of rat VSMCs were conducted , using VSMCs in passage4‐7 for the following experiments .The VSMCs were divided into 4 groups:control group ,Hcy (100μmol/L) group ,Hcy+ rosuvastatin group ,and Hcy+ rapamycin group .MTT was used to investigate the proliferation of VSMCs .Transwell chambers and wound healing were employed to test the migration ability of VSMCs . ICC was used to detect the VSMCs'morphology .Western blotting was used to investigate the expressions of SM‐actin ,SM‐MHC ,calponin ,OPN ,and p‐P70S6K1 in VSMCs of each group .Results Compared with those in control group , the proliferation and migration ability of VSMCs were significantly increased in Hcy modulation group (P< 0 .01) .The expressions of SM‐MCH and calponin increased but those of OPN and p‐P70S6K1 decreased in Hcy group compared with control group (P<0 .01) .The expression of SM‐actin did not significantly differ between the groups .Compared with those in Hcy modulation group , the proliferation and migration ability of VSMCs were significantly decreased in rosuvastatin and rapamycin groups ( P<0 .01) .The expressions of SM‐MCH and calponin increased while OPN and p‐P70S6K1 expressions decreased in rosuvastatin and rapamycin groups compared with Hcy group ( P< 0 .01 ) . Conclusion Hcy can induce the dedifferentiation of VSMCs ,and rosuvastatin can inhibit this effect of Hcy .Its potential mechanism may be realized via the mTOR/P70S6K1 signal pathway .

Objective To evaluate the clinical feasibility of performing the placement of drainage tube via transnasal route in treating mediastinal anastomotic fistula after surgery of esophageal cancer.Methods A total of 6 patients with mediastinal anastomotic fistula after surgery of esophageal cancer,who were admitted to authors' hospital during the period from August 2015 to January 2016,were included in this study.The diagnosis was confirmed by esophageal radiography and thoracic CT scan in all the 6 patients.Under X-ray monitoring,the drainage tube was inserted into the cavity of mediastinal fistula with the help of a guide wire guidance,and continuous negative pressure suction was adopted.The jejunum nutrition tube was inserted via the same nostril if feeding tube was not placed.Results Successful placement of fistula drainage tube and jejunum nutrition tube was achieved in all the 6 patients.In one patient the fistula drainage tube had to be re-placed as the drainage tube was obstructed five days after initial placement.Under X-ray monitoring the mean time used for the drainage tube placement was 33 min (range of 23-48 min).The procedure was well tolerated by all the 6 patients,and no procedure-related complications occurred.After continuous negative pressure suction that lasted for 6-40 days (mean of 23 days) the fistulae healed.Conclusion For the treatment of mediastinal anastomotic fistula after surgery of esophageal cancer,the placement of fistula drainage tube under X-ray monitoring is simple,safe and effective.(J Intervent Radiol,2017,26:250-252)

Objective To establish experimental radioactive wound model in miniature pig and observe its pathological changes in order to carry out in vivo study of radioactive wound-related diseases.Methods According to the random number table,eight adult Bama miniature pigs were divided into four groups after being anesthetized:normal control and experiment groups exposed to 0,10,20 and 30 Gy respectively using the X-ray linear accelerator.Then a puncher was use to prepare full-thickness skin defects (4 cm in length) in the irradiation site.Wound samples were collected every other week to observe wound healing.RT-PCR was used to measure expressions of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β in wound tissues 35 days after irradiation.At the same time,Western blot was utilized to detect expressions of CD31 and collagen Ⅰ.HE staining was applied to observe histopathological changes of the radioactive wound.Results Skin wounds in normal control group healed in 35 days.Whereas in 20 and 30 Gy experiment groups,the wounds were not healed in 63 days and the healing rate was gradually weakened with the irradiation dose increasing.Expression ratio of TNF-α mRNA was 0.315 ±0.007 in normal control group,0.984 ±0.211 in 10 Gy experiment group,1.140 ±0.088 in 20 Gy experiment group,and 0.471 ±0.030 in 30 Gy experiment group.Expression ratio of TGF-β mRNA was 0.402 ± 0.018 in normal control group,1.707 ± 0.147 in 10 Gy experiment group,1.933 ± 0.078 in 20 Gy experiment group,and 0.968 ± 0.084 in 30 Gy experiment group.Expressions of TNF-α and TGF-β mRNA were significantly increased in 20 Gy experiment group compared to normal control group (P ＜ 0.05).Expressions of CD 31 and Collagen Ⅰ protein were inhibited in 20 Gy experiment group compared to normal control group (P＜0.05).Epithelialization and granulation tissue regeneration in 20 Gy experiment group were impaired.Conclusion An irradiation wound model in Bama miniature pigs can be efficiently and effectively simulated using the 20 Gy X-ray irradiation combined with full-thickness skin defect,and can be used to study the pathophysiology and therapeutic intervention of radioactive wound-related diseases in vivo.

Objective To investigate the expression of anchor attachment protein(AAP)in colorectal carcinoma tissues and serum level of AAP in colorectal carcinoma patients and to identify the relationship among AAP expression,lymph node and liver metastases.Methods Immunohistochemistry was used to detect AAP expression,and ELISA was used to test AAP level in peripheral vein blood in 83 patients with colorectal carcinoma.Results The expression rate in normal colorectal mucosa,primary cancer,lymph nodes and liver metastases were 20.5%、53.0%、69.8% and 80.0%,respectively.The positive rate of AAP in primary cancer,lymph nodes and in liver metastasesfoci was higher than that in normal mucosa(P

Objective To study the influence of hyponatremia on prognosis in hospitalized patients with chronic heart failure . Methods A total of 322 patients with chronic heart failure from Feb .2006 to Aug .2012 were retrospectively reviewed and random-ly divided into hyponatremia group(n=161) and normal serum sodium group(n=161) .The clinical data of the two groups were compared .Results There were significant difference between the two groups in the BNP levels ,length of stay ,hospital mortality and readmission rates(P<0 .05) .Serum sodium concentration in hyponatremia group was decreased with the decrease of cardiac function(P<0 .05) ,BNP levels was elevated with the decrease of blood sodium level (P<0 .05) ,days of hospitalization and hospital mortality were increased with the decrease of cardiac function (P< 0 .05) .Conclusion Patients with heart failure combined hy-ponatremia have poor cardiac function ,higher in-hospital mortality and readmission rates and longer hospital stay .

Objective To investigate the long-term excessive drinking semen nitric oxide (NO) content in their sperm quality and spermatogenic cell apoptosis and infertility. Methods Nitrate reductase method was used to specific reduce the NO metabolites nitrate (NO3-) to nitrite (NO2-), which was used on behalf of the total NO level. Terminal deoxynucleotidyl transferase (TdT) and mediated nick end labeling (TUNEL) method and binocular optical microscope were used to detect and observe the rate of apoptosis of spermatogenic cells and the morphological structure. The SQA-V sperm quality automatic analyzer was used to measure sperm quality. Results In not drinking semen fertility group, NO content was (54.81±11.45)μmol / L, the rate of spermatogenic cell apoptosis was (4.52±1.23)%, sperm motility was (80.24±0.17)%, energy a+b (78.32±0.12)%, deformity rate (5.30±0.13)%, and long-term excessive alcohol infertility C group was [(128.83±22.73)μmol/L,(17.34±2.53)%,(51.18±0.58)%,(21.45±0.26)%,(21.12±3.24)%] respectively. Compared to a very significant difference (t＝10.04,17.38,6.69,15.59,17.02,P＜0.01) . In long-term excessive drinking group, the levels of NO and spermatogenesis cell apoptosis rate was significantly positive correlated (r＝0.93,P＜0.01).Apoptosis of spermatogenic cell nucleus chromatin was condensed in the formation of crescent-shaped perinuclear, nuclear was cleavaged to form apoptotic bodies. Conclusions In the long-term excessive drinking semen, NO content and spermatogenic cell apoptosis rate was increased, the sperm had poor quality. The results showed that the long-term excessive drinking can cause germ cell apoptosis in male infertility and promote the body to overproduce NO, whichmay be one of the reasons for male infertility.