Objective To study on the expressions of IL-8mRNA,MCP-1mRNA and MIP-1mRNA and the counts of TAM and MC and their correlation in ovarian adenocarcinoma tissues.Methods detecting the expressive levels of IL-8mRNA,MCP-1mRNA In situ hybridization were derected,and MIP-1?mRNA and Envision immunohistochemistry wes used for the counts of TAM and MC.Results The positive rates of IL-8mRNA,MCP-1mRNA and MIP-1?mRNA expressions and the counts of TAM and MC were significantly lower in the cases of histologic grade G1,clinical stage Ⅰ+Ⅱ,no-metastasis of regional lymph node,and no-infiltration of peritumoral tissues than those in the ones of histologic grade G3,clinical stage Ⅲ+Ⅳ,metastasis of regional lymph node,and infiltration peritumoral tissues(P

Objective The purpose of this study was to determine and compare the hemodynamic effects of desflurane and enflurane in children.Methods Twenty-eight ASA Ⅰ-Ⅱ patients, aged 1-5 years were randomly assigned to receive either desflurane (group D) or enflurane (group E) for maintenance of anesthesia.Cardiac output was continuously monitored by transesophageal Doppler (TECO1).The stroke volume index (SVI), cardiac index (CI),heart rate (HR) and blood pressure(SBP,DBP,MAP) were determined before and during inhalation of desflurane and enflurane at 0.5,1.0 and 1.5 MAC.Results At 0.5 MAC of both agents SVI, CI and HR remained unchanged, but SBP, DBP and MAP were slightly reduced as compared to the baseline values.At 1.0 MAC of desflurane CI was increased and was higher than that in the group E. SVI and HR were significantly higher in group D than those in group E at 1.0 MAC.SBP, MAP and DBP were further reduced and the magnitude of reduction was similar between both groups.At 1.5 MAC CI and SVI were unchanged in group D but greatly reduced in group E.HR increased significantly in group D but remained unchanged in group E.There was further decrease in BP in both groups but SBP and MAP decreased more markedly in group E than in group D .Conclusions Desflurane causes less hemodynamic changes in children of 1-5 years than enflurane.

BACKGROUND: Rat model of renal transplantation is the best way for studying human rejection following renal transplantation. However, the utilization of this model is limited due to refined surgical technique.OBJECTIVE: To explore a microsurgery operation method for constructing a stable, reliable orthotopic renal transplantation model.DESIGN, TIME AND SETTING: The animal observation experiment was performed at the animal laboratory of Tianjin Union Medicine Centre from September to November 2008.MATERIALS: A total of 40 SD rats, weighing 250-300 g, were medially adopted as donators and recipients. METHODS: The renal vessels were separated by blunt separation. The kidneys of donators were infused, removed and conserved at low temperature. To the recipients, the left kidney was removed, and renal blood vessel was anastomosed by ext-ext anastomosis and reconstructed urinary passage by implanting ureter into bladder. 6-point acupuncture was used to suture wound. MAIN OUTCOME MEASURES: The rationality and the authenticity of this operation were evaluated through analyzing the operative time, postoperative death rate and the cause of death.RESULTS: The total time of operation was (150±17) min, with warm ischemia time of (15±2) s and (55±5) min cold ischemia time. Three rats died at the first week after operation, which caused by anesthetic accident, stomatorrhagia and ureterostenoma. The achievement ratio of operation was 85%. At day 8 after operation, exploratory laparotomy of survived rats showed that the transplanted kidney was recovered well without embolism or stenosis at the stoma.CONCLUSION: An orthotopic renal transplantation model of rat is constructed by ext-ext anastomosis and reconstructed urinary passage by implanting ureter into bladder.

OBJECTIVETo compare therapeutic effects between patella replacement and patella osteotomy in total knee arthroplasty.

METHODSFrom April 2004 to April 2011, 52 patients (54 knees) were enrolled in the clinical trail of total knee arthroplasty, who received patella replacement (24 knees, including 13 males and 11 females,ranging in age from 53 to 78 years old or patella osteotomy (30 knees,including 16 males and 12 females,ranging in age from 55 to 79 years old. The average follow-up period was 56 months,ranging from 20 to 80 months. The American HSS Score for knee, the Feller score for patella, range of motion (ROM) for knee, patient satisfaction and complications related to the patella were used to evaluate therapeutic effects.

RESULTSIn the patella replacement group,the preoperative and final follow-up HSS scores of patients were 38.4 +/- 8.2 and 91.2 +/- 8.6 respectively; Feller scores were 13.6 +/- 6.2 and 25.2 +/- 4.2; scores of anterior knee pain were 3.9 +/- 3.2 and 11.2 +/- 3.7; ROM were (78 +/- 26) degrees and(108 +/- 18) degrees. In the patella osteotomy group,the preoperative and final follow-up HSS scores of patients were 39.5 +/- 8.4 and 91.0 +/- 8.5 respectively;Feller scores were 13.4 +/- 6.5 and 25.6 +/- 4.0; scores of anterior knee pain were 3.7 +/- 3.1 and 11.3 +/- 3.6; ROM were (76 +/- 27) degrees and (110 +/- 19) degrees. In the patella replacement group,patient's satisfaction was 91%, and complication related to the patella was 16.7%; in the patella osteotomy group, patient's satisfaction was 89%, and complications related to the patella was 10.0%. There were no statistically significant differeneces in final follow-up HSS scores, Feller scores, scores of anterior knee pain and ROM between the two groups. However,there was no significant difference of patient's satisfaction between them. There was statistically significant differenece of patella-related complications between the two groups, and the complication rate in the patella replacement group was higher than that in the patella osteotomy group.

CONCLUSIONTotal knee arthroplasty with patella replacement or patella osteotomy dramatically relieves pain and improves the knee function. Patella-related complications are associated with its treatment methods, but post-operative anterior knee pain and patient's satisfaction are not related to treatment methods of the patella.

Aged ; Arthroplasty, Replacement, Knee ; adverse effects ; Case-Control Studies ; China ; Female ; Humans ; Knee Joint ; surgery ; Male ; Middle Aged ; Osteotomy ; adverse effects ; Pain, Postoperative ; epidemiology ; etiology ; Patella ; surgery ; Treatment Outcome

OBJECTIVE:To observe the clinical efficacy and safety of piperazine ferulate combined with glutathione in the treatment of diabetic nephropathy. METHODS:80 patients with diabetic nephropathy in our hospital were divided into observation group and control group according to random number table,with 40 cases in each group. Both groups was given general treatment as blood glucose,blood lipid and blood pressure control. Control group was additionally given Reduced glutathione tablets 400 mg, tid,on the basis of general treatment. Observation group was additionally given Piperazine ferulate tablets 100 mg,tid,on the ba-sis of control group. Both groups were treated for 12 weeks. The fasting plasma glucose(FPG),2 h postprandial plasma glucose(2 hPG),blood pressure,blood lipid,serum creatinine (Scr),blood urea nitrogen (BUN),24 h urinary total protein and albumin, urine β2 microglobulin(β2-MG)and N-acetyl-β-glucosaminidase(NAG)of 2 groups were detected before and after treatment. The occurrence of ADR was observed. RESULTS:Before treatment,there was no statistical significance in FPG,2 hPG,blood pres-sure,blood lipid,Scr,BUN,24 h urinary total protein and albumin,urine β2-MG and NAG between 2 groups(P>0.05). After treatment,above indexes of 2 groups were improved significantly,and the observation group was better than the control group, with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:In the treatment of diabetic ne-phropathy,piperazine ferulate combined with glutathione can improve blood glucose,blood pressure,blood lipid levels and renal function with good safety.

Objective: To study network pharmacology mechanism of main ingredients of Guanxinning Injection treating cardiovascular diseases by computer simulation. Methods: Danshensu, salvianolic acid B, protocatechuic aldehyde, rosmarinic acid, tanshinone IIA, lithospermic acid, ferulic acid, senkyuno lide I, ligustrazine, butylphthalide, and ligustilide from Guanxinning Injection were used to predict and screen the targets by reverse molecular docking technology, and were used to study pharmacological mechanism of main ingredients of Guanxinning Injection treating cardiovascular diseases relying on protein-protein interaction network, GO biological processes enrichment, and KEGG signaling pathways enrichment. Results: There were total 11 active ingredients acting INS, Akt1, TNF, MAPK1, ESR1, F2, SERPINE1, and 142 cardiovascular diseases related targets in Guanxinning Injection. These targets were mainly involved in steroid hormone mediated signaling pathway, glutathione metabolic process, positive regulation of smooth muscle cell proliferation and other biological processes, and regulation of coagulation, inflammation and immune, endocrine, and 20 relevant pathways. Conclusion: Guanxinning Injection participated in the treatment of the cardiovascular diseases by anti-inflammatory, anti-oxidant, anticoagulation, promoting fibrinolysis, regulating hormones, and maintaining cardiovascular homeostasis.

OBJECTIVE: To analyze active ingredients,targets,pathways and biological processes of xianglian pills by means of network pharmacology and explore its pharmacodynamics material base and mechanism of action.METHODS: Active ingredients and corresponding targets of xianglian pills were obtained by using TCMSP database and BATMAN-TCM analytical platform,the network of xianglian pills active ingredients-targets was constructed by using Cytoscape 3.5.1 software, using the STRING platform to build protein-protein interaction; GO bioprocess enrichment and clustering analysis of target genes were finished by using BINGO and MCODE plug-ins; KEGG signaling pathways of target genes were analyzed by means of DAVID database.RESULTS: Eleven active ingredients in xianglian pills act on 291 potential targets. these targets participate in eighteen major GO biological process adjustment sub-clusters of the inflammatory response, leukotriene metabolism, lipid metabolism, apoptosis, smooth muscle contraction and platelet activation etc. involve in thirty-three signal pathways of seven categories: inflammation, metabolism,signal transduction, cancer, secretion of digestive juices, ardiovascular system etc.CONCLUSION: The different therapeutic value, pharmacodynamic substances and molecular mechanism of Xianglian pills in the treatment of inflammation,diabetes,cancer and cardiovascular diseases have been preliminarily elucidated, which provides new clues for the development of its clinical application.

OBJECTIVE: To discuss anti-depression mechanism of baihe zhimu tang using network pharmacology. METHODS: The reported antidepressant active ingredients in baihe zhimu tang were used to predict the targets of main active ingredients of baihe zhimu tang relying on traditional Chinese medicine pharmacology system technology platform(TCMSP)database, reverse molecular docking server(DRAR-CPI) and annotation of the human genome database(GeneCards). The Cytoscape 3.5.1 software was used to construct the baihe zhimu tang active ingredients-targets network. The STRING platform was used to construct protein-protein interaction network, and biological information annotation databases(DAVID)was used to analyze metabolic pathways and biological processes of the targets. RESULTS: There are total 11 active ingredients and 106 anti-depression related targets in the baihe zhimu tang. The 106 targets were mainly involved in regulation of neurotrophin, FoxO, insulin, MAPK, PI3K-Akt, prolactin and proteolysis, signal transduction, protein phosphorylation and RNA regulation relevant biological processes. CONCLUSION: Baihe zhimu tang may play a role in the intervention of depression by acting on the relevant neural, hormonal targets and pathways.

OBJECTIVETo explore effects of color Doppler ultrasound and magnetic resonance imaging (MRI) in diagnosis intramuscular hemangioma of skeletal muscle.

METHODSFrom December 2000 to January 2013, 54 patients treated by operation confirmed as intramuscular hemangioma of skeletal muscle by pathology postoperatively, there were 19 males and 35 females aged from 11 to 59 years old (averaged 33.6); the courses of disease ranged from 2.5 to 15 years with an average of 5.2 years. Thirty-eight patients were checked by color Doppler ultrasound, and 14 patients were inspected by MRI. All patients were treated by operation. Postoperative operative time, blood loss in operation, and complications and pathology postoperatively were observed, and IMH clinical effective evaluating standard were used to evaluate clinical outcomes.

RESULTSForty-three patients were followed up from 7 to 49 months with an average of 28.4 months. Operative time was (53 to 187) min with average of 76.3 min, blood loss was (70 to 350) ml with an average of 223.6 ml. No infections and death occurred. Thirty-five patients were diagnosed by color Doppler ultrasound and 13 patients were confirmed by MRI. Twenty patients were capillary type, 22 patients were spongy vascular type and 12 patients were mixed type according to Brown pathological type. In accordance with IMH clinical effective evaluating standard, 29 cases obtained excellent results, 8 moderate and 4 dissatisfaction and 2 poor.

CONCLUSIONColor doppler ultrasound and MRI get a high rate diagnosing patients with intramuscular hemangioma and have an significant valuable in clinical application, and surgical operation which has advantages of relieve symptoms obviously, improve life quality and reduce recurrence rate, could receive good curative effect.

Adolescent ; Adult ; Child ; Female ; Hemangioma ; diagnosis ; pathology ; surgery ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Muscle, Skeletal ; pathology ; Ultrasonography, Doppler, Color

BACKGROUND:Human umbilical vein endothelial cells transfected with lentivirus containing enhanced green fluorescent protein can be easily traced. The optimal multiplicity of infection and time for producing strong fluorescence intensity can lay the foundation of tracing human umbilical vein endothelial cells in animal models.
OBJECTIVE:To observe expression of lentivirus containing enhanced green fluorescent protein in human umbilical vein endothelial cells, and thereby to find a stable method to label human umbilical vein endothelial cells.
METHODS:Using 0.1%col agenase perfusion digestion, we isolated human umbilical vein endothelial cells, which then were placed into a culture medium containing 20%fetal bovine serum and endothelial cellgrowth factor and observed under an inverted microscope. Fol owing digestion, centrifugation and suspension, the cells were counted and divided into four groups, 5.0×105 cells in each group. After cells were seededonto 24-wel plates, 10μL serum-free Dulbecco’s modified Eagle’s medium was added into the blank group, and lentiviruses containing enhanced green fluorescent protein were added into another three groups for celltransfection respectively at multiplicities of infection of 2, 3, 4. There were three dishes in each group.
RESULTS AND CONCLUSION:After cultured for 5-7 days, isolated cells grew into a single layer and exhibited a cobblestone-like arrangement under a light microscope. In addition, factor VIII related antigen test was positive. A green fluorescence was visible at 24 hours of transfection, and peaked at 72 hours. Transfection efficiency was in a linear growth with the multiplicity of infection. Up to the 21st day of transfection, the green fluorescence was stil visible. After 0, 7, 14, 21 days of transfection, the number of human umbilical vein endothelial cells showed no difference between the transfection group with the multiplicity of infection=3 and blank group, suggesting the proliferative ability of cells has no changes after transfection with lentivirus containing enhanced green fluorescent protein. These findings indicate that the lentivirus containing enhanced green fluorescent protein can highly transfect human umbilical vein endothelial cells, and green fluorescent protein can sustainably express for 21 days but cannot impact the cellproliferation.