Objective To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice. Methods HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg?kg-1?d-1) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL. Results Dexamethasone repressed the growth of cervical cancer and induced tumor cell apoptosis, but the tumor volumes were of statistical difference between the two groups of different injection start time (P

Objective To investigate the effects of over physiological dose of dexamethasone on cervical carcinoma cell xenografted tumors in nude mouse.Methods HeLa cells were inoculated subcutaneously in BALB/c female mice aged 5-6 weeks.Over physiological dose of dexamethasone(62.5 mg?kg-1?d-1) was injected into the peritoneum from the 16th day after inoculation for 10 d.Diameters of tumor were measured every two or three days.The volumes and the inhibition rates of tumor were calculated.Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL.VEGF and TNF-? were detected by immunohistochemistry.Results In vivo,over physiological dose of dexamethasone can repress the growth of tumor and induce cell apoptosis.Compared with the control group,the tumor sizes were significantly smaller(P0.05).Conclusion Over physiological dose of dexamethasone can repress cervical tumor growth in vivo.This action may be related to apoptosis and VEGF,but have no relationship with TNF-?.

Objective To study the perioperative management of abdominal surgery in patients aged over 80 years. Methods The cliuical data of 25 patients aged over 80 years undergoing abdominal surgery were analysed retrospectively. Results Of the 25 patients, 8 subjected to biliary surgery, 13 gastiic cancer or cardiac cancer surgery, 1 esophageal cancer surgery, 3 intestinal surgery. 24 patients(96%) were cured; one died postoperalively.Conclusions In over aged patieats, they have more coexite diseases, and more postoperative complications, so extensive perioperative management, choice of selective operation, reducing of emergent operation, and giving albumin to help the recovery of tissues are essential.

Objective To evaluate the imaging features of patients with vascular cognitive impairment (VCI) induced by Subcortical Ischemic Vascular Disease (SIVD), through magnetic resonance diffusion tensor imaging ( DTI ) and proton spectroscopy ( MRS ) technology. Methods A total of 52 patients with SIVD were enrolled. After analysis of scale score, 32 patients with cognitive impairment were assigned to VCI group and 20 patients with no cognitive impairment were assigned to control group. Both group received DTI and MRS examination. The mean values of apparent diffusion coefficient (ADC) and fractional anisotropy (FA) of the bilateral temporal, frontal, parietal and occipital white matter regions as well as in the bilateral centrum semiovale were calculated. The peak value of MRS of N-acetyl aspartate (NAA) , choline ( Cho), creatine (Cr) and phaseomannite ( mI ) were calculated. Results Compared with control group, FA decreased in the region of temporal, frontal, parietal as well as in the centrum semiovale , and ADC increased in VC[group ( P ＜ 0.05 ) . In the frontal regions and centrum semiovale,the VCI patients had a significant FA decrease. The ADC value increased obviously in the temperal lobe.Spectrum analysis results showed, NAA/Cr was lower than control group in VCI group in the frontal lobe (1.43 ±0.08 vs 1.53 ± 0. 92), while mI/Cr was higher than control group in the temporal lobe(0. 51 ±0. 06 vs 0. 46 ± 0. 07 ) ( P ＜ 0. 05 ). Conclusion FA in the temporal and centrum semiovale regions of VCI group and NAA in the temporal white matter regions decreased obviously. DTI and MRS could provide a reference value for early diagnosis and assessment of VCI.

Our laboratory isolated three defensin molecules from the circulating neutrophils of healthydonors.The three molecule mixture was conjugated with BSA and immunized New Zealand rab-bit.ELISA showed that the rabbit antisera was of a very high titters,which were specific for hu-man defensins.Immunoperoxidase staining with this rabbit antisera indicated that normal humanneutrophils,acute myeloid leukemia cell lines KG1 and HL-60 were highly positive.In con-trast,normal mononuclear leukocytes,erythrocytes,erythroleukemia cell line K562 were all neg-ative,even rabbit and guinea pig neutrophils had no response to this rabbit anti-human de-fensins antisera.Our preliminary experiments suggested that the rabbit antisera was highly spe-cific for human defensins.Probably,defensin is a differentiation antigen of granunocyte lineage,and antibody against human defensins would be a candidate immunological reagent specific for a-cute myeloid leukemia.Further work for this should be done.

Adenomatoid Tumor ; metabolism ; pathology ; surgery ; Adult ; Biomarkers, Tumor ; metabolism ; Female ; Humans ; Hysterectomy ; methods ; Keratins ; metabolism ; Lymphangioma ; pathology ; Uterine Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Objective To explore the relationship of a polymorphism c.757T ＞ C in C-reactive protein gene (CRP) and carotid artery atherosclerosis in patients with ischemic cerebrovascular disease (ICVD).Methods A case-control study investigated 276 ICVD patients and 125 controls matched for age and sex.Genotypes of-757T/C in CRP were analyzed in patients with ICVD using polymerase chain reaction-restriction fragment length polymorphism.The level of serum C-reactive protein was detected by using immune scatter turbidimetry and the intima-media thickness of common carotid artery and internal carotid artery were measured by color Doppler ultrasonography.The relationship between the polymorphism -757T/C in CRP and carotid atherosclerosis was assessed.Results The genotypes of the polymorphism -757T/Cin CRP in the ICVD group (207/276(75.0％),65/276(23.6％),4/276(1.4％)) was significantly different from the control group (75/125 (60.0％),46/125 (36.8％),4/125 (3.2％) ; x2 =9.531,P =0.008),the frequence of T allele in patients with ICVD (479/552(86.8％)) was increased significantly compared to the control group (196/250 (78.4％)),the frequence of C allele in the ICVD group (73/552(13.2％)) was decreased significantly in contrast to the control group (54/250 (21.6％) ;x2 =9.056,P =0.004).Among the patients with ICVD,the level of serum C-reactive protein in patients with-757TC/CC genotype was elevated significantly than that with-757TC/CC ((4.2± 1.6) mg/L vs (2.7 ± 1.9) mg/L; t =5.900,P ＜ 0.01).Logistic regression analysis demonstrated that the-757C allele in CRP was related to the carotid artery atherosclerosis (OR =1.379,95％ CI 0.852-0.864,P =0.023).Conclusions The polymorphism-757T/C in CRP maybe related to the risk of developing ICVD.The frequency of the-757C allele can increase the level of serum C-reactive protein.And this polymorphism may be associated with carotid artery atheroselerosis in patients with ICVD.

Objective To investigate the methods of rectifying the excessive width of fold line and conglutination of the upper eyelid following blepharoplasty. Methods The width of double eyelid was redesigned, ranging from 6 mm to 8 mm. Then the scar of upper eyelid was excised about 1 mm to 2 mm, and the orbital septum fat flap, orbicularis oculi flap, infraorbicularis oculi fat flap and injection of lipochondria were utilized to fill the depression of upper eyelid. Results In our series, there were 20 eyelids of 16 cases receiving rectification. Orbital septum fat flap was used to correct 2 eye-lids, orbicularis oculi flap 4 eyelids, infraorbicularis oculi fat flap 10 eyelids and injection of lipochon-dria 4 cases. Among 12 eases of following-up, 10 of them were satisfied with the postoperative effects. The results were acceptable in other 2 cases. Conclusion It is a good approach to correct the excessive width of fold line and conglutination of the upper eyelid with local flaps, including the orbital septum fat flap, orbicularis oculi flap, infraorbicularis oculi fat flap and injection of lipochondria.

Objective To evaluate the effect of combination splenectomy and endoscopic varices ligation in comparison with Hassab procedure in the treatment of portal hypertension. A prospective, controlled study was carried out on Splenectomy with EVL in comparision with portoazygous disconnection--the Hassab procedure to assess whether SEVL can achieve better results in the treatment of portal hypertension. Methods From Jan 1999 to June 2002, 103 cirrhotic patients with portal hypertension were admitted. These patients were randomized into two groups. Group A were treated by splenectomy combined with EVL(53 cases) , and group B were treated with Hassab procedure(50 cases). Results In both groups, there was a significant postoperative decrease in free portal pressure, the velocity and volume of portal flow (all P0.05). Portal vein thrombosis developed in 7 cases (13%) in group A, and in 14 cases (28%) in group B, P

Objective To study the influence of total flavonoids of hemerocallis fulva(TFHF) on hepatocyte apoptosis and related protein expression in mice with alcoholic hepatic injury.Methods A total of 40 mice were randomly divided into four groups:blank control,model control andsmall and high dose TFHF groups,10 cases in each group.The mice were given the continuous gavage administration for 7 d.Then the model group was given once gavage by 50％ ethanol 12.0 mL/kg after 1 h of the last administration.The blank control group was given the equal volume of distilled water.The activity levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum as well as the superoxide dismutase(SOD) activity and malondialdehyde (MDA) content in liver tissue hemogenate were detected.Hematoxylin and Eosin(HE) staining was performed for observing the pathological changes of the liver tissue.The flow cytometer was used to test the apoptosis ratio in hepatocyte suspension.The expressions of caspase-3,Bcl-2 and Bax protein were detected by Western blot.Results The various TFHF groups could decrease the activities of ALT and AST in serum (P＜0.05),while could decrease the MDA content in liver tissue hemogenate (P＜0.01) and increased the SOD activity;the liver tissue pathological examination showed that the high dose TFHF group could make the liver cell degeneration,alleviated the necrosis degree and relieved the pathological change of hepatic tissue;compared with the model group,the hepatocyte apoptosis rate in each TFHF group was decreased significantly;Western blotting results showed that the caspase-3 protein level in each TFHF group was decreased,expression of Bcl-2 protein was increased,whereas which of Bax protein was decreased and Bax/Bcl-2 ratio was reduced.Conclnsion TFHF has obvious protective effect on mice acute hepatic injury induced by ethanol,and can inhibit the hepatocyte apptosis,its action mechanism may be related to its antioxidation and regulation of caspase-3,Bcl-2 and Bax expression.