Objective To explore the relationship between the amount of CD3+ T cells in gastric cardia carcinoma(GCC)samples and the clinical pathological index of GCC.Methods Immunohistochemical Elivision method was used to detect CD3+T cells in 42 cases of GCC samples,18 cases of paraneoplastic tissue and 12 cases of normal cardiac mucosa tissue.The relationship between CD3+ T cell and the clinical pathological index was analysed.Results(1)The order of the mean number of CD3+ T cells from high to low was paraneoplastic tissue,GCC and normal mucosa(P0.05).(3)The mean number of CD3+ T cells was negatively correlated with the depth of carcinoma invasion and the lymphatic metastisis(P

Objective To study on the expressions of IL-8mRNA,MCP-1mRNA and MIP-1mRNA and the counts of TAM and MC and their correlation in ovarian adenocarcinoma tissues.Methods detecting the expressive levels of IL-8mRNA,MCP-1mRNA In situ hybridization were derected,and MIP-1?mRNA and Envision immunohistochemistry wes used for the counts of TAM and MC.Results The positive rates of IL-8mRNA,MCP-1mRNA and MIP-1?mRNA expressions and the counts of TAM and MC were significantly lower in the cases of histologic grade G1,clinical stage Ⅰ+Ⅱ,no-metastasis of regional lymph node,and no-infiltration of peritumoral tissues than those in the ones of histologic grade G3,clinical stage Ⅲ+Ⅳ,metastasis of regional lymph node,and infiltration peritumoral tissues(P

The DNA microarray study showed that in STZ-induced diabetic rats the elevatedexpression of 42 genes in artery were depressed markedly after atorvastatin treat ment .This suggeststhat atorvastatin may have the protective effects on the diabetic vascular lesion.

OBJECTIVE:To probe into the integrity of package insert for 75 kinds of commonly used antiinfective agents for injection in our hospital and to provide reference for rational use of antiinfective agents for injection. METHODS:According to Drug Description Specification and Management Regulation for Drug Description and Label,the terms included in package insert for antiinfective agents for injection were analyzed statistically. RESULTS:The package inserts for 75 kinds of antiinfective agents for injection were enrolled. The proportion of integral items were high while that of some terms related to clinic were low such as dosage and usage,medication for children,over dosage and skin test. The descriptions of PK,pharmacology,toxicdogy, ADR,et al. were very simple and different. CONCLUSION:It is suggested that items about over dosage and medication for special population should be detailed as well as usage so that package insert can guide rational use of drugs in the clinic.

Objective To describe a minimal invasional surgical procedure for the treatment of avulsion fracture of tibial intercondylar eminence. Methods From July 1999 to December 2000, 26 cases of avulsion fracture of tibial intercondylar eminence were treated. With the arthroscope, reduction and absorbable suture fixation of anterior cruciate ligament or the avulsion fracture were performed. Taking the advantage of ACL－ aimer, bone tunnels, through which the suture can be pulled out and tied outside the joint, could be accurately produced. Results Twenty-two patients were available for 2－ 17 months(mean 10.5 months) follow-up. Seventeen patients gained normal range of motion, 5 have limited flexion but exceeded 90 degrees. X－ ray demonstrated bone union in all the cases and anatomical reduction obtained in 21 cases. Conclusion Arthroscopically guided operation, gives less trauma, and is a simple and effective method for the avulsion fracture of the tibial intercondylar eminence.

Objective To investigate the imaging feature of Chinese familial BCS, and to evaluate the efficacy of interventional treatment for Chinese familial BCS. Methods Angiography was taken in 4 familial BCS patients(from families A and B), and PTA was attempted in 2 of 4 patients, stents were placed in another 2 patients. Results Sisters in family A and sisters in family B had membranous obstruction of the inferior vena cava(MOVC) and segmental obstruction of the inferior vena cava(SOVC), respectively. PTA was attempted in younger sisters of the two families, stent was implanted in elder sisters of the two families. Patency of inferior vena cava and clinical symptom improvement were noted in 4 patients after interventional treatment. Restenosis was detected by angiography in sisters of family A after follow up 2 years later, and stenting was performed in the elder sister, PTA was performed in the younger sister once again. The elder sister was dead after the second stenting, and the younger sister had no symptom up to now. Sisters in family B had no restenosis after 4 years′ follow up. Conclusion 1.Lesion types of Chinese familial BCS were varied. 2.Vena cava thrombosis is the etiology of Chinese familial BCS .3.PTA, stenting and long time anticoagulation treatment may be effective methods in the treatment of familial BCS.

Objective To study the correlation between organizational commitment of nurses and their intention to drop out. Methods Toally 304 contract nurses were recruited in this investigation. The questionnaires including demographic data, scale of organizational commitment and intention to drop out scale were used. Results The score of intention to drop out was 16.94 ± 2.16. The score on organizational commitment was 71.19 ± 6.65. There was a positive correlation between intention to drop out and organizational commitment among the contract nurses. Conclusions Improving contract nurses′organizational commitment can be an effective mean to reduce contract nurses′intention to drop out. It is an important means to promote the development of the nurse team and provide a reference for the scientific development of nursing.

BACKGROUND:Due to special physiological and anatomical location, stability of the spine is very complicated during thoracolumbar fractures. It is difficult to identify the stability of the spine. It should be based on their individual circumstances, to explore more effective internal fixation repair method.
OBJECTIVE:To explore the Cobb’s angle and vertebral height of patients with thoracolumbar fracture and spinal cord compression treated with posterior laminectomy and screw fixation, and compared with anterior laminectomy.
METHODS:One hundred patients with thoracolumbar fracture and spinal cord compression, who were treated in the Panyu District Central Hospital from January 2013 to November 2014, were enroled in this study. The patients were equaly and randomly divided into posterior laminectomy fixation group and anterior laminectomy fixation group. Tactile and sports of American Spinal Injury Association scores, Cobb’s angle and vertebral height were assessed before treatment and 1 month after treatment, and fixation effects were compared between the twogroups.
RESULTS AND CONCLUSION:(1) No significant difference in each index was detected between the two groups preoperatively (P> 0.05). (2) Tactile and sports of American Spinal Injury Association scores, Cobb’s angle and vertebral height were better in the posterior laminectomy fixation group than in the anterior laminectomy fixation group at 1 month postoperatively (P< 0.05). (3) These findings indicated that compared with the anterior laminectomy fixation, posterior laminectomy fixation for thoracolumbar fracture combined with spinal cord compression obtained better outcomes, and could obviously relieve spinal cord compression. Posterior laminectomy fixation isasafe and effective treatment method for thoracolumbar fracture and spinal cord compression.

AIM: To analyze the associated factors of poor vision after phacoemulsification operation.
METHODS:Clinical data of cases with poor vision after cataract phacoemulsification combined with IOL implantation from 341 patients(418 eyes) during Jan. 2015 to Jan. 2016 in our hospital were collected and logistic correlation analyzed.
RESULTS: The incidences of postoperative low vision were 10. 3% for one month after operation. Forty-three eyes were reported low vision, and 41 of 43 the patients with postoperative low vision had fundus oculi disease before operation, which included 20 eyes ( 48. 8%) with age-related macular degeneration, 16 eyes(39. 0%) with diabetic retinopathy, 3 eyes ( 7. 3%) with glaucomatous optic neuropathy, 1 eye(2. 4%) with pathologic myopia of retinopathy and 1 eye ( 2. 4%) with branch retinal vein occlusion.
CONCLUSION: The associated factors of poor vision after phacoemulsification operation are preoperative eye diseases and the nuclear hardness, and preoperative eye diseases may be the main causes of postoperative low vision. Comprehensive examination should be attached importance to, and be careful in the operation to decrease the harm for visual performance.

BACKGROUND: Donor complications have been detected following autologous tendon transplantation for posterior cruciate ligament reconstruction. Although artificial tendon development and tissue-engineered tendon have achieved great progresses, there are some issues in clinical application. Since 1980's, allogenic tendon transplantation has aroused increasing attention. OBJECTIVE: To explore the selection of allogenic tendon materials and the effect of their application on reconstructing posterior cruciate ligament. METHODS: A total of 17 patients with posterior cruciate ligament injury of knee joint were treated with cryopreserved allogenic tendon by Tibial-inlay technique. During the operation, two tracts of tendons soaked in gentamicin saline for 15 minutes were conduplicated, and one end of the tendon was cancellous bone screw and fixed to the tibia attachment point of posterior cruciate ligament, and the other end was introduced into the joint through retention suture. The posterior joint capsule was repaired. The patient was placed at supine position, and the knee was flexed for 90°. The other end of the graft was introduced to femoral tunnel, and anterior drawer was tensed, and fixed by screw. RESULTS AND CONCLUSlN: The preoperative posterior drawer test of patients was >2+, including 7 cases of 3+ and 6 of 4+. The postoperative posterior drawer test was 0 in 4 cases, 1+ in 8 cases, 2+ in 4 cases and 3+ in 1 case, suggesting the posterior movement of the knee joint was significantly improved. Lysholm scores of patients were (48.5±4.3) points before operation and (88.3±5.4) points after operation. Results show that cryopreserved allogenic tendon by Tibial-inlay technique could restored function of posterior cruciate ligament with a favorable effect.