Animals ; Disease Models, Animal ; Heart Failure

Animals ; Evidence-Based Medicine ; Health Knowledge, Attitudes, Practice ; Humans ; Infant ; Meta-Analysis as Topic ; Milk Hypersensitivity ; diagnosis ; prevention & control ; Milk Proteins ; adverse effects ; Practice Guidelines as Topic ; standards ; Professional Staff Committees ; organization & administration

Actins ; metabolism ; Carcinosarcoma ; metabolism ; pathology ; Craniopharyngioma ; pathology ; Diagnosis, Differential ; Esthesioneuroblastoma, Olfactory ; pathology ; Humans ; Immunohistochemistry ; Keratins ; metabolism ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Nasal Cavity ; Nose Neoplasms ; metabolism ; pathology ; Paranasal Sinus Neoplasms ; metabolism ; pathology ; Pituitary Neoplasms ; pathology ; Teratoma ; metabolism ; pathology ; Vimentin ; metabolism

Objective To assess effects of cerebral ischemic postconditioning(IPost)on neuronal apoptosis and phosphated glycogen synthase kinase-3β(GSK-3β)after cerebral ischemic/reperfusion.Methods The experiment was conducted at the center for animal experiment of Renmin Hospital,Wuhan University.Thirty male Wistar rats were randomly divided into three groups:sham operation(S),ischemic/reperfusion(I/R)and ischemic postconditioning(IPost).Each group contained ten rats.Global brain ischemia was produced with four-VO method.Animals were killed after two days.Apoptosis in neurons in the cortex region were detected by TUNEL assay; infarct areas were detected by TTC ; activity of p-GSK-3β was detected by spectrum assay; Statistical software SPSS13.0 was applicated to perform one-way ANOVA Student-Newman-Keul test; correlation was detected by linear regression.Results Compared with group S,TUNEL-positive cells and infarct areas increased(P ＜0.01),the activity of p-GSK-3β decreased in I/R and IPost groups(P ＜ 0.01).Compared with group I/R,TUNEL-positive cells and infarct areas significantly decreased(P ＜ 0.01),the activity of p-GSK-3β increased in group IPost(P ＜ 0.01).The activity of p-GSK-3β and TUNEL-positive cells had highly correlation,so as infarct areas(P ＜ 0.01).Conclusions IPost can lessen the ischemic/reperfusion injury of Cortex,through increas the activity of p-GSK-3[β and decreasing neuronal apoptosis.

The emerging technologies change rapidly in the medical field, refresh the medical ethical scenari-os, brought some medical ethical blind spot.Therefore, building the platform of academic research, making use of the new media, MOOC and“flipped classroom”, inheriting the native“The Spirits of A Great Physician” in tradi-tional Chinese medicine and western medicine, life ethics education and sublation of new exploration, the ideolog-ical and moral education for medical students is expected to be improved in China.

Objective To observe the effects of pentabrominated diphenyl ether (BED-99) on the histological structure and function of the thyroid of rats. Methods Forty SPF, SD rats aged 4 weeks, were randomly divided into 4 groups according to the body weight,10 in each group. The rats were treated with BED-99 at the doses of 30 mg/kg,60 mg/kg,120 mg/kg,respectively, through gavage. The rats in the control group were received the equal volume of corn oil. The levels of serum hormone were determined,the change of histopathology,histochemistry and follicular cells ultramicroscopic structure were observed at 15 days of exposure to BED-99. Results Serum levels of thyroxin (FT4),triiodothyronine (FT3),decreased gradually with dose increase at 15 days of exposure to BED-99.However,thyroid stimulating hormone (TSH) levels increased. The thyroid follicular epithelium hyperplasia was aggravated gradually with dose increase. Proliferating cell nuclear antigen (PCNA) positive cells increased constantly with dose increase. The activities of thyroperoxidase (TPO) and succinate dehydrogenase (SDH) of the treated rats increased significantly compared with the controls,and appeared color darkened. The rough endoplasmic reticulum of the thyroid follicular cells changed to saccate under the electron microscope,whereas nucleolus,cell membrane and mitochondria were not injured. Conclusion BDE-99 may damage the histological structure and function of the thyroid, the mechanism may be that BDE-99 down-regulates the thyroglobulin synthesis and then induces thyroxin level decrease which causes an increase of TSH through feedback.

Objective To ob se rve the effects of danazol on hypothalamus-pituitary-gonad axises and expressi ons of hypothalamic GnRH and pituitary GnRH receptor mRNA in female rats. Methods Female rats at 5 days of age were given a sin gle subcutaneous injection of 300 ?g danazol to induce true precocious puberty. Animals were divided into 3 groups: normal control, model group and vehicle gro up. The day of vaginal opening from 25 days of age was monitored, and the establ ishment of first estrus cycle from the day of vaginal opening was investigated b y examination of vaginal smears. The weights of body, pituitary, adrenal, uterus and ovary were measured to identify the developmental status of main organs. Th e expressions of hypothalamic GnRH and pituitary GnRH receptor mRNA were assayed by semi-quantitative RT-PCR. Results Female rats treated with danazol significantly (P

Objective In order to evaluate the quality and performance of chlorine dioxide disinfectant,it is necessary to develop a set of methods to determine the components in chlorine dioxide disinfectant.Methods Iodometry,malonic acid iodometry,improved malonic acid iodometry and five-step iodometry were employed to determine the components including ClO_2, Cl_2,ClO_2~-,and ClO_3~-in chlorine dioxide disinfectant,the advantages and disadvantages of the test methods were compared.Results Using iodometry,the liquid stabilized chlorine dioxide products activated by hydrochloric acid,the content of ClO_2 was 20.23 mg/ml that was accord with the content which was marked in the product label.Using malonic acid iodometriy,the liquid stabilized chlorine dioxide contents of ClO_2 was 19.99 mg/ml,Cl_2 was 0.35 mg/ml,in which a little chlorine was detected.Using improved malonate iodometry and five-step iodometry,the activation rates of the liquid stabilized chlorine dioxide products were 88.0% and 75.6% respectively,ClO_2 and ClO_2~-in the liquid stabilized chlorine dioxide products could be distinguished.Conclusion Active chlorine dioxide and residual ClO_2~-can be detected by using iodometriy and improved malonic acid iodometry,but iodometriy and malonic acid iodometry can not.

Objective To observe the effects of exogenous sodium phosphocreatine (PCr) on cerebral repeffusion injury of rats after ischemia in order to explore the potential mechanism. Method Thirty-six healthy adult male Wistar rats with body weight 200- 220 g were randomly (random number) divided into sham operation group, ischemic reperfusion (I/R) group and PCr treatment group. The I/R model was established by using electro-cauterizing bilateral vertebral arteries and occluding bilateral common carotid arteries with atraumatic carotid clasps for 10 min, and then the clasps were released for 48 hours reperfusion. In sham operation group, bilateral common carotid arteries were exposed without occlusion. In PCr treatnent group, PCr in dose of 150 mg/kg was administered intravenously 60 min before the occlusion of bilateral common carotid arteries. Normal saline was administered intravenously instead of PCr into rats of I/R group. After reporfusion for 48 hours, the rats were sacrificed and brains removed for detections of neuron apoptosis by using TUNEL, malondialdebyde (MDA) level by using chromtometry and calmodulin (CaM) activity by using ELISA. Results Compared with sham operation group, TUNEL-positive cells, MDA level and CaM activity increased in I/R group and PGr treatment group ( P ＜0.01). Compared with I/R group, TUNEL-positive cells, MDA level and CaM activity were lower significantly in PCr treatment group ( P ＜ 0.01). Conclusions PCr can lessen cerebral ischemic reperfusion injury and neuron apoptosis, the mechanism maybe relates to the attenuation of abnormalities in calcium balance and reduction of oxygen free radicals by PCr.

Objective To investigate the relationship between the expression of β-glucuronidase mRNA in hepatocellular carcinoma(HCC)tissue and the clinicopathologic factors.Methods The expressions of β-glucuronidase mRNA in 25 samples of HCC tissue and 10 samples of normal hepatic tissue were detected by RT-PCR,and the relationship between β-glucurenidase mRNA in HCC tissue and clinicopathologic factors was analyzed.Results The expression of β-glucuronidase mRNA in HCC tissue was not influenced by the age of patient,tumor size and alpha-fetoprotein level.The expression of β-glueuronidase mRNA in patients with portal vein tumor thrombus or lymph node metastasis Was significantly higher than those without(t=7.857,9.341,P＜0.05).Conclusions The expression of β-glucuronidase mRNA is closely related to the existence of portal vein tumor thrombus and lymph node metastasis.β-glucuronidase may play a role in the invasion and metastasis of HCC.