OBJECTIVE: Aseptic prosthetic loosening is a primary problem of total hip arthroplasty. For this reason, several prosthesis interfaces with different characteristics have been studied, such as ceramic-on-ceramic prosthesis. The purpose of this study wasto investigate the mid- and short-term curative effects of ceramic-on-ceramic total hip arthroplasty in treatment of avascularnecrosis of the femoral head (ANFH) and to evaluate its clinical application value.METHODS: A total of 23 patients (28 hips) with ANFH who received ceramic-on-ceramic non-cement hip prosthesis replacementwere followed up. All hip prostheses were BiCONTACT~((R)) ceramic-on-ceramic hip prostheses. Functional evaluation was assessedaccording to the Harris hip score. Radiographic analysis was performed using the Gruen's zonal method for the stem and the zonal classification of Delee-Charnley for the cup implants.RESULTS: All patients were followed up for an average of 28 months. The mean Harris hip scores ranged from 39.45 points prior to surgery to 88.57 points after surgery, with a difference which was significant (P < 0.05). Radiographically, no obvious prosthesis loosening or subsidence was observed. CONCLUSION: The ceramic-on-ceramic total hip arthroplasty can produce good short-term curative effects in treatment of ANFH.Radiographic findings do not show obvious prosthesis loosening.

Objective To compare the application effects of pentobarbital sodium ,urethane and midazolam combined with ket-amine/Sumianxin Ⅱ in rabbit bone defect repair .Methods 120 healthy male adult rabbits were randomly divided into three groups according to different anesthetic agents ,40 cases in each group .The group A was intramuscularly injected by pentobarbital sodium , the group B was given urethane by the ear vein and the group C was given midazolam combined with ketamine/and Sumianxin Ⅱ by intramuscular injection .The onset time of anesthesia ,maintenance time ,breathing rate ,heart rate ,complications ,recovery time ,ad-ditional anesthetic agents and postoperative death rate in 3 groups were recorded respectively .Results The onset time of anesthesia in the group B and C was(4 .53 ± 0 .65) min and(3 .56 ± 1 .31) min respectively ,which were significantly shortened compared with the group A(P<0 .05);the maintenance time of anesthesia in the group B was (152 .12 ± 12 .53) min ,which was significantly short-ened compared with the group A and C (P<0 .05);the recovery time in the group C was (31 .05 ± 5 .73) min ,which was significant-ly shortened compared with the group A and B(P<0 .05) ,the propability of additional anesthetic agents and the postoperative mor-tality in the group C were significantly decreased compared with the group A and B (P<0 .05) .Conclusion The combined anesthe-sia of midazolam and ketamine/Sumianxin Ⅱ has definite effect without any observed intraoperative depressions of breathing rate and heart rate and with high safety ,high controllability and less influence on the long term survival of the experimental animals , which is a more ideal anesthesia method of the rabbit bone defect repair .

Composite Resins ; radiation effects ; Light

Objective To comparatively complete clinical effectiveness of laparoscopic and open-bifemoral bypass in the treatment of advanced atherosclerosis.Methods During January 2008 to January 2013,according to the treatment method,a total of 60 patients with advanced atherosclerosis patients were divided into group A and group B,and there were 30 cases in each group,and group A was treated with open master bifemoral bypass surgery,and group B was treated with laparoscopic totally master bifemoral bypass surgery,to compare the general information of the two groups,surgery related indicators,clinical efficacy and survival rate,and to analyze the effect of surgical treatment on the prognosis of patients by COX model.Results The operation time of patients in group B (365.3 ±41.3) min and aortic clamping time (59.5 ± 18.3) min were significantly higher than that of group A,(294.3 ±35.5) min and(26.5 ± 19.3) min (P ＜0.05),bleeding amount (400.0 ± 145.3) ml and length of hospital stay (10.5 ± 2.1) d of group B patients was significantly lower than that of group A (1 002.3 ± 324.3) ml and (16.4 ± 4.3) d (P ＜ 0.05);postoperative mortality,thrombosis rate and the incidence of serious complications of patients in group B were significantly lower than group A (P ＜ 0.05);survival rate at different time of group B was significantly higher than that of group A (P ＜ 0.05);COX model analysis show that the surgical methods affect the postoperative mortality,thrombosis and the occurrence of severe complications.Conclusions Completely laparoscopic master bifemoral bypass can significantly decreased advanced main iliac artery occlusion disease mortality,and has small surgical trauma,less postoperative complications,rapid recovery and other advantages,especially for elderly critically ill patients,and it was worthy of clinical application.

Objective To explore the clinical curative effect of locking plate in elderly patients with proximal humeral fractures. Methods Seventy elderly patients with proximal humeral fractures were selected, and all patients were treated with surgical treatment. Among them, application of locking plate was in 35 cases (locking plate group), and application of ordinary steel plate was in 35 cases (ordinary steel plate group). The operation time, intraoperative blood loss, fracture healing time and curative effect between 2 groups were compared. Results The operation time, intraoperative blood loss and fracture healing time in locking plate group were significantly lower than those in ordinary steel plate group: (61.3 ± 5.6) min vs. (78.3 ± 4.2) min, (190.5 ± 11.5) ml vs. (268.5 ± 21.2) ml and (12.6 ± 1.8) weeks vs. (16.5 ± 1.5) weeks, the excellent and good rate was significantly higher than that in ordinary steel plate group: 94.29% (33/35) vs. 74.98% (26/35), and there were statistical differences (P<0.05). Conclusions The curative effect of locking plate in elderly patients with proximal humeral fractures is accurate. Locking plate can be fixed firmly, and it is conducive to early recovery after surgery.

Objective To properly choose and employ an air disinfect-purifying machine in ICU. Method This paper analyses the principle of air disinfect-purifying machine and put forward some attention-deserved problems such as the value of CADR in clinical application. Conclusion. The disinfect-purifying machine with different CADR value should be chosen according to the area of air disinfect-purifying district, thus can really bring the machine into full play.

BACKGROUND: Construction of lentivirus vector has the advantages of simplicity,it is regard as the most effective and successful method in transgene therapy. OBJECTIVE: To clone the metastasis suppressor gene KiSS-1 from human normal placenta tissue and construct its lentivirus vector. DESIGN,TIME AND SETTING: The open experiment was performed at the Laboratory of Fujian Normal University from September 2006 to December 2007. MATERIAL: The pNL-IRES2-EGFP vector was conservated by the Laboratory of Fujian Normal University. METHODS: Total RNA was extracted from human placenta tissue. The opening reading frame cDNA of KiSS-1 was isolated by using RT-PCR,and cloned into its lentiviral vector pNL-IRES2-EGFP to construct expression plasmid pNL-IRES2-EGFP-KiSS-1. MAIN OUTCOME MEASURES: Clone objective gene fragment of KiSS-1,restriction enzyme digestion and gene sequencing of the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 were observed in the study. RESULTS: The nucleotide sequence isolated from the recombinant plasmid pNL-IRES2-EGFP-KiSS-1 was confirmed the same as expected by restriction enzyme digestion and gene sequencing. CONCLUSION: The recombinant plasmid pNL-IRES2-EGFP-KiSS-1 has been constructed successfully.

Human tissue factor pathway inhibitor (TFPI) is a member of the Kunitz-type serine protease inhibitor family, which is divided into TFPI-1 and TFPI-2. The key function of TFPI-1 is anticoagulate, while TFPI-2 is a broad-spectrum serine protease inhibiter. Both of them are composed of three repeated Kunitz-type domains. Even though sharing some structural homology, they are quite different from each other in terms of the coding sequences, tissue origins and distribution, the functions and the mechanisms involved. These differences between TFPI-1 and TFPI-2 lead to the discrepancies in the roles they played in a variety of physiological and pathological processes. The recent progress in the related research is reviewed in this article.

With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.

Objectives To compare the hemodynamic effects and ventricular activation sequence associated with the respective pacing of the right ventricular outflow tract (RVOT) and right ventricular apex (RVA). Methods 79 patients with complete atrioventricular block (AVB) or sick sinus syndrome (SSS)to undergo permanent ventricular stimulation either at the right ventricular apex (RVA) (n = 38) or right ventricular outflow tract (RVOT) (n =41) were selected. The left ventricular ejection fraction (LVEF),cardiac index (CI), mitral E/A ratio and QRS duration were recorded before implantation of pacemaker and 6 or 12 months after implantation. Results There were no significant differences in CI , LVEF, E/A between RVOT and RVA pacing(P ＞0. 05). Compared with normal sinus rhythm, the QRS duration was significantly lengthened when pacing at RVOT or RVA (P ＜0. 05). RVA pacing was significantly longer than RVOT in QRS duration (P ＜ 0. 05). Conclusions There was no significant difference with the hemodynamic effect between RVOT and RVA pacing in subject with normal heart function. Compared with RVA pacing, RVOT pacing remained relatively normal ventricular activation sequence.