The nature of renal tubular cell injury in ischemic acute renal failure includes not only cell death (necrosis or apoptosis) but also sublethal injury causing cell dysfunction. The role of intracellu- lar calcium, calcium - dependent enzymes calpain, nitric oxide, phospholipase A2, loss of tubule cell polarity and tubular obstruction in the pathophysiology of the renal tubular cell injury during hypoxia/ischemia is described. The effects of vascular factors, infiltrating activited leukocytes, apoptosis and growth factors on renal tubular cell injury are discussed. Potential mechanisms that tubular injury leads to a profound fall in glomerular filtration rate are proposed.

Chromosome Aberrations ; radiation effects ; DNA Damage ; radiation effects ; Electromagnetic Fields ; adverse effects ; Sister Chromatid Exchange ; radiation effects

As an outward, voltage-dependent potassium channel, M type channel is crucial in the regulation of neuronal excitability; it is modulated by a variety of factors in vivo and its dysfunction often results in neuronal system diseases. Great efforts have been made to elucidate the mechanism underlying M channel modulation since its discovery decades ago. It is generally accepted that the Phospholipase C (PLC) signaling pathway plays a significant role in the M channel modulation. This review highlights the relationship between PLC signaling pathway and M channel modulation, as well as some recent progresses in the research of this field.

Objective: To explore a simple and effective method for establishing three dimensional finite element model of the maxillary first premolar, so as to provide a mathematic model for future biomechanic research. Methods: Micro-CT was used to scan the extracted, intact left maxillary first premolar and the obtained images were transferred for use. The point data of the enamel, dentin, and pulp were collected by Mimics 10.0 software and were fed into Imageware 12.0 software for treatment with point cloud data and inverse algorithm of curve. After surface fitting, the tooth solid model was accomplished by Ansys 11.0 software. Then the enamel, dentin, and pulp were integrated through Boolean operation using Ansys 11.0. The solid models of periodontium and alveolar bone were constructed and the 3D meshes were created separately; and the models were tested with loading. Results: A fine three-dimensional finite element model of the maxillary first premolar, including the pulp, periodontium and alveolar bone was established. After 3D meshing, the enamel had 26 685 tetrahedral elements, the dentin had 11 082 tetrahedral elements, the pulp had 11 843 tetrahedral elements, the periodontium had 22 004 tetrahedral elements, and the alveolar bone had 66 767 tetrahedral elements. The model had a total of 241 381 10-node-tetrahedral elements; it had a high precision and exactly demonstrated the morphology of the tooth and the curvilinear shape of enamelo-cementel junction. The stress distribution of enamel focused on the middle of occlusal face and enamelocemental junction; the stress distribution of dentin also focused on the enamelo-cemental junction. Conclusion: A three-dimensional finite element model of maxillary first premolar has been established by combining the Micro-CT technology and reverse-engineering software; the model has satisfactory geometric and mechanical comparability.

Objective To realize fully automatic X-ray exposure controlling,simplify operating processes,and improve the quality of images.Methods We designed 5 or 7 X-ray detectors with silicon batteries,improved film-holding board of the main engine of the X-ray machine,then assembled them by mechanical gearing,and thus made multi-measured-limit,automatically-changed X-ray detectors.Results When choosing film boxes according to the radiographed positions,the detectors will change automatically into measured-limits coinciding with the interested positions.The average density was around 1Ⅴ in 9 randomly selected photographs.High quality was achieved in up to 98% of 10 000 radiographed images for clinic radiography.Conclusion The key of X-ray automatic exposure controlling technology is X-ray detecting technology,by which the quality of image can be improved.

OBJECTIVE:To investigate the study of traditional Chinese medicine (TCM) in the treatment of chloasma,and to provide reference for clinical treatment and study of chloasma. METHODS:Retrieved from CBM,Wanfang database,CNKI and VIP,usingchloasmaas subject,the literatures of TCM therapy were collected during Jan. 2003-Dec. 2012. Bibliometric meth-od was adopted to analyze included literatures statistically in respects of publication amount,periodicals,research institution,litera-ture types,citing references,other periodicals citatien times,fund support,distribution of provinces and cities. RESULTS:During 2005-2012,the amount of the literatures increased slowly. Among top 10 journals in the list of publication amount,only Chinese Journal of Aesthetic Medicine was not TCM journal and took up the first place. Among top 7 research institutions in the list of publi-cation amount,TCM colleges took up predominant place,followed by TCM hospitals. The types of the literatures were mainly case report,contrastive study before and after treatment,group-divided control study;the amount of group-divided control studies increased gradually year by year. The references cited were mainly English literatures. There were 82 literatures which were cited by other periodicals more than 10 times;the highest time of other periodical citation per literature was 47 times;only 11 literatures were supported by fund. The institutions of literatures authors were distributed in 32 provinces,cities and autonomous regions of China,mainly in the eastern coastal areas. CONCLUSIONS:More and more attention has being paid to TCM therapy for chloas-ma,but the scope and depth of the studies were inadequate. From the point of view of modern medicine,we can explore the patho-logical TCM mechanism and the treatment method of shortening the course of disease.

Objective To observe the effects of a novel HLA-derived peptide, RDP1258, on the human peripheral blood mononuclear cell (PBMC) proliferation to ConA and MLR, and to investigate the mechanisms. Methods Peptide RDP1258 was chemically synthesized. The effects of the peptide on alloreactive cytotoxic activities of human PBMCs were observed using 3HTdR incorporation method. RDP1258, HLA-B2702.75-84, and control peptide were administrated respectively in every experiment. The activity of heme oxygenase-1 (HO-1) was analyzed by the enzymochemical method. Results The results showed that the synthetic HLA-derived peptide could obviously inhibit the proliferation of human PBMCs and inhibited HO activity in a dose-dependent manner in vitro. Conclusion HO-1 might participate in the inhibitory effect of RDP1258 on the proliferation of human PBMCs induced by mitogen and isoantigen.

To introduce the international registration condition of acupuncture clinical research. With the examples of World Health Organization International Clinical Trials Registry Platform and the U. S. National Institutes of Health Clinical Registration Platform, the registration method and current condition of acupuncture clinical trials in international clinical trials registration platform were analyzed. The results indicate that the number of acupuncture clinical trials registration is gradually increased and the registration number from China is on the rise as well. But most domestic acupuncture clinical researches haven't been registered arid the researchers' valuing degree for clinical trials registration and methodology research needs to be improved.
Acupuncture Therapy ; standards ; Biomedical Research ; legislation & jurisprudence ; standards ; Clinical Trials as Topic ; legislation & jurisprudence ; standards ; Humans ; Registries

AIM:To investigate the role of JAK-STAT pathway , IL-1βand IL-6 in the PC12 cells with X-ray irradiation.METHODS:The PC12 cells were irradiated with X-ray at doses of 2, 4 and 8 Gy.After 24 h, the levels of IL-1βand IL-6 were detected by ELISA .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 were measured by Western blot .RESULTS:Compared with control group , the levels of IL-1βand IL-6 increased .The protein levels of p-JAK1, p-JAK2, p-STAT1, p-STAT3 and p-STAT5 increased with the doses of X-ray exposed.CONCLUSION:JAK-STAT signaling pathway , IL-1βand IL-6 play a role in the injury of PC12 cells with X-ray irradiation.