Objective To investigate the causes of failure of manually rotating fetal head technology and its delivery outcomes.Methods One hundred and Ninety-eight cases with persistent occipito transverse position or persistent occipitoposterior position admitted to our hospital form January 2008 to December 2010 were enrolled as our subjects.According to the result of manually rotating fetal head technology,these patients were divided into the success group (126 cases) and the failure group (72 cases).The information related to delivery was recorded.Results The proportion of fetal weight ≥ 3500 g in the failure group was 76.4％ (55/72),higher than that of the success group(31.7％ (40/126)),and there has statistic significant difference (x2 =30.177,P =0.001).The proportion of uterine inertia in the failure group(58.3％ (42/72)) was higher than that of the success group(38.1％ (48/126)),and there has statistic significant difference (x2 =7.569,P =0.006).The proportion of critical or low-grade narrow birth canal in the failure group(38.9％ (28/72)) was significantly higher than that of the success group (23.8％ (30/126)),and there has statistic significant difference (x2 =5.030,P =0.002).The proportion of the improper time for manually rotating fetal head (opening of cervix was less than 6 cm,and fetal head was above ischial spine and opening of cervix was 8-10 cm,fetal head reach to more than 2 cm lower of ischial spine) in the failure group was 61.1％ (44/72),which was significantly higher than that of the success group (38.9 ％ (49/126)),and there has statistic significant difference (x2 =9.084,P =0.003).The proportion of maternal and neonatal complications (postpartum hemorrhage,puerperal morbidity,fetal distress,neonatal asphyxia) in the failure group were significantly higher than those of the success group (x2 =9.586,P =0.002 ; x2 =9.334,P =0.002 ; x2 =5.910,P =0.015 ; x2 =5.240,P =0.022).The proportion of cesarean section in the failure group(72.2％ (52/72)) was significantly higher than that of the success group(34.1％ (43/126)),and there has statistic significant difference(x2 =26.641,P =0.001).Conclusion The manually rotating fetal head technology can transform from dystocia to normal delivery and decrease the rate of cesarean section.It is necessary to prevent and deal with the failure causes of manually rotating fetal head technology.And it must be cautious to perform the follow-up correction and trial production after the failure of previous correction.

ObjectiveTo explore the influence of cognitive behavioral intervention on hospitalized patients with schizophrenia.Methods100 hospitalized schizophrenia patients were randomly divided into experimental group and the control group.Routine treatment given to the patients in the control group and cognitive behavioral intervention was applied to the patients at the same time based on routine treatment in experimental group.The patients were assessed with the mini-mental state examination( MMSE),activities of daily living(ADL) and WHO battery of cognitive assessment instrument(WHO-BCAI) pre-and post-cognitive behavioral intervention.ResultsThe expenrentcl geoup and ntrol group before and after the intervention the average MMSE score was(21.8 ± 3.9),(24.4 ± 4.7 ),(21.5 ±3.4),(22.9 ±3.8) ;ADL score an average of(28.8 ±7.9),(25.1 ±4.5),(27.9 ±7.1),(25.2 ±4.8) (t =6.89,11.13,all P ＜ 0.05 ) ; cognitive function in the auditory vocabulary learning,language skills,visual identification,the connection test and cancellation tests were different ( P ＜ 0.05 or P ＜ 0.01 ).ConclusionCognitive behavioral intervention had positive influence on cognitive function in hospitalized patients with schizophrenia.

Histone deacetylase7 (HDAC7) belongs to Ⅱ a histone deacetylases.HDAC7 can alter chromosome structure and regulate gene transcription,and plays an important role in tumorigenesis and tumor angiogenesis.Increasing evidences show that HDAC7 can maintain the vascular integrity and continuity,and regulate the expression of angiogenic genes.HDAC7 also can regulate the migration and proliferation of vascular endothelial cells,and regulate angiogenic effect mediated by VEGF and other proangiogenenic factors contributing to tumor angiogenesis.Therefore,studies of the mechanisms in which HDAC7 contributes to tumor angiogenesis and the development of HDAC7 inhibitors could provide a new direction to tumor diagnosis and therapy.

Objective To construct pEGFP-N1-TGF-β1 recombinant plasmid and transfect it into primary cultured neonatal piglet type Ⅱ alveolar epithelium cell (AEC-Ⅱ) by using lipofectamine 2000,in order to provide basis of methodology for producing recombinant plasmids for transplantation of transfected AEC-Ⅱ into ALI/ARDS animal model lungs.Methods PCR primers were designed to amplify the human TGF-β1 cDNA fragment from plasmid.XhoI and EcoRI were used for double digesting the empty plasmid pEGFP-N1 and cDNA fragment of human TGF-β1.Then the products of double enzyme digestion by using T4 DNA ligase were connected and transformed into DH5α and cultured over night for 16 hours.The structure of recombinant plasmid was identified by using PCR and base sequencing to verify the correctness of pEGFP-N1-TGF-β1 recombinant plasmid.It was then transfected into primarily cultured AEC-Ⅱ by lipofectamine2000 mediated transfection and cultured for another 48 hour.Plasmid DNA (pEGFP-N1-TGF-β1 recombinant plasmid) and lipofectamine 2000 were added into serum-free DMEM respectively,then DNA suspension and Lipofectamine 2000 suspension were blended together and added into cells.After 24-48 hours later,the expression level of enhanced green fluorescent protein (EGFP) was evaluated under fluorescence microscope.Results The structure of vector was verified as pEGFP-N1-TGF-β1 recombinant plasmid by using PCR and base sequencing.Green fluorescence found in some cells showed that the pEGFP-N1-TGF-β1 recombinant plasmids had been successfully transfected into primary cultured AEC-Ⅱ,however,the transfection efficiency still need tobe further improved such as repeating the transfection procedure once again or using adenovirus mediated transfection method to improve the efficiceny and to transplant the cells into animal lungs eventually.Conclusions pEGFP-N1-TGF-β1 recombinant plasmid was successfully constructed and,for the first time,transfected into primarily cultured AEC-Ⅱ of newborn piglets.This established method should be useful for investigation of therapeutic effect and outcomes of lung with experimental acute lung injury.

Objective:To design a new rehabilitation system for cerebral apoplexy patients, and help patients complete rehabilitation treatment on the bed.Methods: Design the basic structure of the system through mechanical technology, make the realization of the function by using circuit and wireless control system, global-eye system was embedded in it by using the principle of the 3G to 4G intelligent technology for improving the humanized design of the system. Results:Through application in physical therapy, nursing, clinical rehabilitation, and clinical therapy in hospitals, it achieved good economic and social benefits, got the recognition of patients, clinicians and rehabilitation therapists. Conclusion: It can be used for the treatment of cerebral apoplexy, reduce medical expenditure, save amount of social resource and improve the work efficiency of medical staff.

Objective:To observe therapeutic effect and influence of rosuvastatin on levels of interleukin (IL)-35 and nu- clear factor-κB (NF-κB)in patients with coronary heart disease (CHD).Methods:A total of 85 CHD patients were randomly divided into rosuvastatin group (n=43,received rosuvastatin calcium therapy based on routine treatment)and atorvastatin group (n=42,received atorvastatin calcium therapy based on routine treatment),both groups were treated for eight weeks all.Cardiac index (CI),cardiac output (CO),left ventricular ejection fraction (LVEF),left ventricular end-diastolic dimension (LVEDd),serum levels of IL-35 and NF-κB,and total effective rate were measured and compared be- tween two groups before and after treatment.Results:Compared with before treatment,there were significant rise in CI, CO,LVEF and IL-35 level,and significant reductions in LVEDd and NF-κB level in both groups after treatment (P<0.05 or<0.01).Compared with atorvastatin group after treatment,there were significant rise in CI [(3.54±0.72)L· min-1 ·m-2 vs.(3.88±0.83)L·min-1 ·m-2 ],CO [(3.78±0.89)L/min vs.(4.94±0.96)L/min],LVEF [(56.20 ±9.71)% vs.(63.48±14.15)%]and serum IL-35 level [(96.26±24.33)pg/ml vs.(106.92±27.26)pg/ml],and sig- nificant reductions in LVEDd [(4.71±0.89)cm vs.(4.36±0.75)cm]and NF-κB level [(21.51±5.01)ng/ml vs. (18.32± 5.17)ng/ml]in rosuvastatin group,P<0.05 all.Total effective rate of rosuvastatin group (95.35% vs. 76.19%)was significantly higher than that of atorvastatin group,P<0.05. Conclusion:Rosuvastatin possesses more sig- nificant therapeutic effect than that of atorvastatin on coronary heart disease,and its heart protection effect besides lipid lowering may be related to regulating levels of interleukin-35 and nuclear factor-κB.

Objective To investigate the effect of medication guide on IL-17,NO,IL-8 and the respiratory function in patients with COPD,and to provide evidence for the prevention and treatment of COPD.Methods From January 2014 to December 2015,100 patients with COPD in our hospital were selected,and 3 months medication guide was given to the patients.The IL-8,IL-17,NO and other oxidative stress index were observed before and after the intervention.Results Before the intervention,the IL-17,IL-8 and NO levels of patients were (77.48 ± 5.48) ng/mL,(216.48 ± 29.40) ng/mL,(59.74 ± 10.38) mol/L respectively.After the intervention,the IL-17,IL-8 and NO levels were (28.57 ± 3.94) ng/mL,(43.80 ± 3.69) ng/mL and (78.62 ± 11.49) μmol/L.The levels of IL-17 and IL-8 were significantly decreased after the intervention,the level of NO was significantly increased (t =7.49,4.83,6.59,all P ＜ 0.05).Before the intervention,the levels of FEV1,FVC and FEV1/FVC were (50.24 ± 3.27) ％,(1.24 ± 0.38) L and (53.52 ± 2.37) ％ respectively.After intervention,the levels of FEV1,FVC and FEV1/FVC were (74.46 ± 5.74) ％,(1.99 ± 0.41) L and (83.27 ± 3.25) ％ respectively.The respiratory parameters of the patients after intervention were significantly increased (t =6.38,3.89,7.02,all P ＜ 0.05).After 3 months of intervention,in 100 patients,7 cases of grade 0,33 cases of grade Ⅰ,54 cases of grade Ⅱ,6 cases of grade Ⅲ,through statistical analysis,compared with those before intervention,significantly improved (x2 =6.49,all P ＜ 0.05).Conclusion After 3 months of intervention,the patients' respiratory function limit improved,the condition improved significantly,and the medication guide for the patients with COPD has a positive effect.

Objective To evaluate the efficacy of MRCP in the determination of common bile duct (CBD) exploration in gallstone pancreatitis(GP). Methods The clinical data of the patients who underwent CBD exploration due to gallstone pancreatitis during two different periods were retrospectively reviewed. Between 1995 and 1999,46 patients with gallstone pancreatitis underwent CBD explorations based on the preoperative ultrasonographic examination of stones and dilatation of CBD . Between 2000 to 2002,the explorations were carried out in 30 patients based on MRCP findings. Results Negative exploration occurred in 28 patients (60.8%) during the first period and 7 patients (23.3%) during the second period. The difference of negative CBD exploration rate between the two groups was statistical significance(P

Purpose:To study LPS induced iNOS mRNA expression and NO production by murine peritoneal macrophages, and their relationship with cytotoxic function of activated macrophages.Methods:LPS induced iNOS mRNA expression, production of NO and effects of activated macrophages on anti tumor were investigated separately by RT PCR, nitrate reductase method and 3HTdR incorporation.Results:LPS can upregulates iNOS mRNA expression in a dose dependent manner. L NIL(specific inhibitors of iNOS) pretreatment significantly reduced cytotoxic effect on the proliferation of HL 60 cells, but had little effect on the K562 tumor target cells on condition that NO production is sufficiently inhibited. Conclusions:LPS can effectively induce iNOS mRNA expression and NO production in murine peritoneal macrophages. NO is one of the important effector molecules in unspecific immunity of activated macrophages, but it plays different roles in cytotoxic effects of macrophages on different target tumor cells.

OBJECTIVE:To study the clinical pharmacokinetics of Midazolam.METHODS:Fourteen patients undergoing general anesthesia with Midazolam in combination with remifentanil and isoflurane were enrolled in our study.The midazolam was administered by constant-rate infusion of 20 ?g?kg-1?min-1 for 20 min.The sampling of radial artery blood samples(3 mL/sample)were schedule before the infusion and at 1,3,5,7,10,15 and 20 min after the initiation of infusion and at 5,15,30,45 min and 1,2,4,6,12,18,24 h following the completion of midazolam.The plasma midazolam concentrations were determined by RP-HPLC.The pharmacokinetic analysis was performed using DAS2.1.1 program.RESULTS:The concentration-time curves of midazolam were best described by a simple three-compartment open model in which the weight coefficient was 1 and the typical pharmacokinetics parameters of midazolam were as follows:t1/2?=9.584 2 min,t1/2?=85.367 7 min,t1/2?=339.736 5 min,V1(the distribution volume of central compartment)=0.182 1 L?kg-1,CL=119.434 4 mL?h-1?kg-1,K10=0.045 8 min-1,K12=0.086 3 min-1,K21=0.017 5 min-1,K13=0.013 3 min-1,K31=0.002 4 min-1.CONCLUSION:t1/2?,t1/2?,t1/2? and CL were in line with those reported in the literature abroad,but V1 is on the lower side and it is easy for the medication to reach a balance.However,the rapid clearance of midazolam is more in line with the pharmacokinetic parameters of the Chinese subjects.