AIM:To investigate the effect of suramin concentration changes on trabeculectomy in rabbit, and to provide treatment strategies for glaucoma on the basis of experiment.
METHODS:Thirty-two albino rabbits were randomly divided into four groups, including standard control group, experimental group Ⅰ, experimental group II, and experimental group Ⅲ. Each eye was performed standard trabeculectomy. During surgery, standard control group was given a piece of cotton with 0. 3mg/mL mitomycin C ( MMC ) for 2min, and the other three groups were given a piece of cotton with 0. 3, 0. 4, and 0.5mg/mL suramin respectively for 2min. The filtering blebs and intraocular pressure ( IOP ) were observed at the 3, 7, 15, and 30d after surgery. Some conjunctiral specimen were observed with hitochemicall ( HE staining) and immunohistochemicall methods.
RESULTS:At postoperative 7, 15, and 30d, the changes of the IOP, functional filtering blebs, and the number of positive cell nuclear in experimental group II and experimental group Ⅲ were significantly different compared with those in standard control group and experimental group Ⅰ (all P<0. 05), and the differences between experimental group Ⅰ and standard control group were not significant (P>0. 05). The changes of the IOP and the number of positive cell nuclear in experimental group Ⅲ were significantly different compared with those in experimental group II (P<0. 05), whereas the differences in functional filtering blebs between experimental group Ⅲ and experimental groupII were not significant (P>0. 05). The status of filtering channel in experimental groupII and experimental group
Ⅲ were better than those in experimental group Ⅰ and standard control group.
CONCLUSION: The concentration of suramin has a significantly influence on its effect. When the concentration is 0. 3mg/mL, the antiproliferative effect of suramin is no more than that of MMC. The effect of 0. 4, 0.5mg/mL suramin is better than MMC. 0. 5mg/mL suramin has a better effect on controlling IOP and suppressing the growth of fibroblasts than 0. 4mg/mL suramin.

Carcinoma, Squamous Cell ; diagnosis ; Humans ; Maxilla ; pathology ; Maxillary Neoplasms ; diagnosis ; Neoplasm Metastasis ; Spinal Neoplasms ; secondary

The growth and pigment production of 47 strains on casein medium were studied comparatively.T4 and Neurospora crassa AS 3.1602 were selected for their capacity of producing melanin on five different culture media. Melanin produced by T4 was investigated, and T4 was identified as Proteus mirabilis primarily.

Actins ; metabolism ; Carcinosarcoma ; metabolism ; pathology ; Craniopharyngioma ; pathology ; Diagnosis, Differential ; Esthesioneuroblastoma, Olfactory ; pathology ; Humans ; Immunohistochemistry ; Keratins ; metabolism ; Male ; Middle Aged ; Mucin-1 ; metabolism ; Nasal Cavity ; Nose Neoplasms ; metabolism ; pathology ; Paranasal Sinus Neoplasms ; metabolism ; pathology ; Pituitary Neoplasms ; pathology ; Teratoma ; metabolism ; pathology ; Vimentin ; metabolism

Adult ; Aged ; Constipation ; drug therapy ; etiology ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Male ; Middle Aged ; Phytotherapy ; Stroke ; complications

Alveolar Process ; Housing ; Humans ; Incisor ; Maxilla ; Tooth Root

Objective To explore the anxious condition of nurses and correlative factors,and to explore the relationship between the nurses' anxiety and the correlative factors,so that can help the management echelon and nurses to correctly understand the relationship of anxiety and pressure,and control and handle the anxiety problem of nurses fundamentally,and improve the physical and mental health,work efficiency and nursing quality of nurses. Methods A questionnaire investigation was conducted in 85 nurses from a certain hospital in Harbin about the anxiety,life events,work stressor and the relationship between them. Results 30 nurses had anxiety(the anxiety group),another 55 nurses showed no anxiety(the nonanxiety group).The comparison of items in life events inventory showed that great difference existed in pregnancy,addition of new family members,the difficulty of training their children,property damage or being stolen,living apart with the spouse,magnificent changes of the life style,having got severe illness or wound.In the aspect of work sttessor scale,professional and career issues,workload and allocation of time,working environment,patient care and interaction showed significant difference.The work pressure was significantly associated with the level of anxiety. Conclusions Our finding suggest that effective stress management strategies should be implemented to reduce or eliminate work pressure of nurses,carry out training to improve the self-regulation ability of nurses,and if necessary biofeedback therapy can be introduced.

BACKGROUND:Lumbar spinal stenosis can severely affect the activities and functions of the lower limbs, and even cause paralysis. OBJECTIVE:To evaluate the clinical effect of intervertebral bone graft with internal fixation for the treatment of lumbar spinal stenosis. METHODS:The lumbar spinal stenosis patients treated with posterior decompression and intervertebral bone graft with internal fixation were fol owed-up. The effect of intervertebral bone graft with internal fixation for the treatment of lumbar spinal stenosis was evaluated through assessing the clinical symptoms and improvement of signs, recovery of neurological function, as wel as intervertebral bone graft and fusion and the occurrence of complications. The effects of posterior decompression and intervertebral bone graft with internal fixation and simple decompression for the treatment of lumbar spinal stenosis were compared, in order to further determine the treatment of intervertebral bone graft with internal fixation. RESUTLS AND CONCLUSION:The fol ow-up results of the lumbar spinal stenosis patients treated with posterior decompression and intervertebral bone graft with internal fixation showed that the symptoms of low back pain, partial limb sensory disturbances and intermittent claudication were improved significantly;the neurological recovery score could be up to 90%or above. Radiographic examination showed good intervertebral bone graft and fusion with less complication of nerves, blood vessels and dural injury. Comparative study showed that the excel ent and good rate of posterior decompression and intervertebral bone graft with internal fixation can reach to 95.2%, while the excel ent and good rate of simple decompression was 23.8%, indicating that the effect of posterior decompression and intervertebral bone graft with internal fixation was significantly better than that of simple decompression.

0.05);the body weight of 170 and 357 mg/kg 3,4-DCA exposure groups decreased significantly(P

To investigate the mechanism of detrusor instability, using an apparatus to measure energy regulated tonicity and length of muscle strip in vitro , detrusor instability in bladder outlet obstruction (BOO) and spinal cord injuries (SCI) after pharmacological intervention was studied. The detrusor of both groups contracted under definite tension. Contraction of DI strips occurred under lower tension and under same tension the frequency of contraction was higher in DI detrusor than the normal one. There were no obvious changes when the detrusor strips were treated with M receptor antagonists, ATP or VIP. Muscarinic receptor agonist could increase the excitability of detrusor. The increase of detrusor excitability plays a major role in the occurrence of DI. The excitability is independent on neurogenic factors.