1.Progress of new-generation genome editing mediated by engineered endonucleases.
Chinese Journal of Biotechnology 2015;31(6):917-928
Genome editing refers to the experimental methods to targeted modify specific loci in the genomic DNA sequence. In recent years, engineered endonucleases, including ZFN, TALEN and CRISPR/Cas, have been developed as a new-generation genome editing technique, and greatly improved the feasibility of gene function analyses, gene therapy, etc. Here, we briefly summarize the basic principle, developmental process and applications of this technology.
Clustered Regularly Interspaced Short Palindromic Repeats
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Endonucleases
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genetics
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Genetic Engineering
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methods
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Genetic Therapy
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Genome
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Genomics
2.Dr. GUAN Zun-hui's Clinical Experience in Treating Simple Obesity by Acupuncture
Journal of Acupuncture and Tuina Science 2003;1(6):6-8
Objective The clinical effects of acupuncture and massaging abdomen with salt the treatment of simple obesity was observed. Methods: Eighty cases were randomized into two groups: treatment group in which 40 cases were treated by acupuncture and massaging abdomen with salt and control group in which another 40 cases were treated by conventional acupuncture. Their effects were observed and compared. Results: The clinical cure rate and marked effective rate in treatment group were significantly higher than in control group (P<0.01). Conclusion: The clinical effects in the treatment group are much better than those in the control group.
3.On the medical students' Humanism-Oriented Education In human's full scale development
Na AN ; Xiao-Bo WANG ;
Chinese Medical Ethics 1994;0(06):-
The theory of human's full scale development is an important content of the Marxism theory.It is the need of human's full scale development theory to strengthen the humanism - Oriented Education of the medical students.Under the situation of constructing the new medicine education pattern,we should consider thoroughly the necessity of strengthening the humanism - Oriented Education of the medical students and discusses profoundly the effective way to strengthen the humanism - Oriented Education of the medical students,thus improve the humanities quality of the medical students in the essence.
4.On Strengthening Education of Medical Students' Behavior Criterions Consciousness
Xiao-Bo WANG ; Na AN ;
Chinese Medical Ethics 1995;0(04):-
Contemporary medical college students' misconducts are so serious that it is urgent to strengthen their behavior criterions consciousness,for example,the sense of rules of law,codes of ethics,school disciplines,medical norm.Schools should do more to strengthen consciousness,make an effort to the daily management,actively guide students to participate in social practice.
5.Peroxisome proliferator-activated receptors and lung diseases
Journal of Medical Postgraduates 2004;0(01):-
Peroxisome proliferator-activated receptors(PPAR) belong to the nuclear hormone receptor family.They have been implicated as important regulators of various physiological processes,such as lipid and lipoprotein metabolism and inflammation.This article reviews the structure of PPARs,their activity regulation and ligands and their correlation with lung diseases.
7.The present status and prospect of antiepileptic drugs
Chinese Journal of Neurology 2021;54(1):5-8
Epilepsy is one of the five major neuropsychiatric diseases on which the World Health Organization focuses. New treatment methods are emerging, such as neuromodulation and radiofrequency ablation to remove lesions and precise treatment for gene mutation. However, antiepileptic drug therapy is still the first choice mostly. Since potassium bromide was applicated in epilepsy in 1857, which opened the prelude to the development of modern antiepileptic drugs, more than 20 kinds of antiepileptic drugs have been used in clinical practice. This article reviews the present status and future development of antiepileptic drugs at home and abroad.
8.Survival and Pathway of Regenerative Motoneurons after Brachial Plexus Root Injury
Chinese Journal of Rehabilitation Theory and Practice 2010;16(11):1031-1033
ObjectiveTo observe the survival and pathway of regenerative axons of spinal motoneurons following root injury of brachial plexus (BPI) and to investigate the correlation between the pathway of regenerative axons and the recovery of motor function. Methods48 adult male SD rats were randomly divided into four groups: crush group, cut group, avulsion group and the control group. 2 months later, the functional recovery was evaluated with Grooming test. Fluorogold labeled regenerative motoneurons retrogradely when injected into the motor or sensory branch of musculocutaneous nerve. 3 d later, the C5-8 spinal cord were harvested and the regenerative motoneurons were counted. The survival neurons were counted with neutral red staining. ResultsThe number of survival neurons in the cut group (1005±89) were more than that of the avulsion group (881±76), but less than the crush group (1126±25). The control group was the most (1900±39). The regenerative motoneurons reinnervated motor branch were much more than that of the sensory branch in all groups. For the Grooming test, the grade in the crush group (3.42) was much higher than that of the avulsion (1.75) and cut (1.75) group. ConclusionRegenerative axons of spinal motoneurons reinnervate the motor branch after BPI. The number of survival neurons is different after different injuries, which associated with the motor functional recovery.
10.Advance in studies on effect of Glycyrrhizae Radix et Rhizoma in relieving purgative activity of Rhei Radix et Rhizoma.
China Journal of Chinese Materia Medica 2015;40(4):577-581
Rhei Radix et Rhizoma, as a commonly used traditional Chinese medicine, has been widely applied in clinic. Its major purgative constituent is anthraquinones, which are believed to be a toxic ingredient. Glycyrrhizae Radix et Rhizoma has been reputed as the best alexipharmic to moderate medicine natures. In this paper, the effect of Glycyrrhizae Radix et Rhizoma in relieving purgative activity of Rhei Radix et Rhizoma was studied in two aspects--the boiling process and intestinal metabolism; Studies on combined administration of Glycyrrhizae Radix et Rhizoma and Rhei Radix et Rhizoma in recent years were summarized according to chemical constituent, intestinal flora, I/II phase metabolism and drug transport. However, the material basis and mechanism for their compatibility remain unclear, further studies will be made in the future.
Animals
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Cathartics
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adverse effects
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analysis
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pharmacology
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Chemistry, Pharmaceutical
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Drugs, Chinese Herbal
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adverse effects
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analysis
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pharmacology
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Glycyrrhiza
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chemistry
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Humans
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Rheum
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adverse effects
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chemistry
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Rhizome
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chemistry