Objective To investigate the drinking water quality of secondary water supply in Tongzhou District, Beijing in order to establish a data monitoring management system. Methods The uniform questionnaires were used for the on-the-spot investigation at secondary water supply units in the whole district, which included secondary water supply facility situations, environmental sanitary conditions and health management and so on. At the same time, municipal water and secondary supply water were tested. Results 48 units of secondary water supply were investigated. In this investigation, the main systems of secondary water supply were the low water tank added frequency conversion pump(accounts for 77.1%) and the inverter constant pressure water supply system with negative pressure(accounts for 14.3%). According to the hygienic requirements, the unqualified rate of water tank structure, disinfection facility and water tank room were 81.0%, 73.3% and 87.3% respectively; besides, 39.6% of the units used the invalid hygienic licenses, 43.5% of the water managers had no health certificates, and 54.5% of the units had never cleaned their water tanks. The total qualification rate of the secondary water supply was 66.7%. The main items that were not up to the standard were ammonia-nitrogen, free chlorine, recognizable objects with naked eyes, total bacteria count and turbidity. For the different water material, the qualification rate showed a significant difference(P=0.023). Conclusion The drinking water quality of the secondary water supply is lower than before and the preventive hygiene supervision and evaluation is comparatively delayed in the investigated district.

Objective To observe and analyze the oral and maxillofacial appearance of patients with acquired immune deficiency syndrome(AIDS)and to explore the effective detection methods of hunman immunodeficiency virus(HIV)infection,aimed at early diagnosis of HIV infection and AIDS,providing reference for treatment and prevention.Methods 21 6 cases of suspected HIV infec-tion and AIDS therapy-naive patients in the department of infection disease in this hospital,from January 2012 to March 2014,were selected.Oral and maxillofacial appearances,detection methods and its results were retrospectively analysed.Results The main clinical manifestations of patients with HIV infection and AIDS were oral candidiasis,oral hairy leukoplakia,herpes simplex virus infection,Kaposi′s sarcoma,periodontal disease,ulcer disease and other abnormal attribute.All of the 21 6 samples were positive in the preliminary screening,214 cases were confirmed by western blotting(WB),and the sensitivity,specificity and false positive rate were 99.07%,100.00% and 0.926%,repectively.Conclusion Focusing on clinical symptoms and signs in oral and maxillofacial re-gion of patients with HIV infection and AIDS could provide significant references for clinical diagnosis,and combing detection meth-ods of enzyme linked immunosorbent assay(ELISA)and WB could make a definite diagnosis,which might be benefit to make effec-tive prevention and treatment measures and in order to control the infection and improve the prognosis.

BACKGROUND:It has been demonstrated that shortening of femoral eccentricity would lead to decreased abductor muscle strength,and overlengthening eccentricity would increase the risk of femoral neck prosthesis fracture and the compressive stress of cemented femoral stem prosthesis after artificial hip replacement,which easily causes prosthetic loosening and subsidence.OBJECTIVE:To investigate the importance of eccentricity adjustment in the artificial hip replacement for treatment of femoral neck fracture in elderly patients.DESIGN,TIME AND SETTING:An observational study was performed at the Department of Orthopedics,First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine between January 2004 and December 2005.PARTICIPANTS:A total of 30 patients with femoral neck fracture,12 males and 18 females,averaging 73.8 years of age (range 62-90 years old),received treatment at the Department of Orthopedics,First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine and were recruited into this study.METHODS:All patients underwent artificial hip replacement operated by the same team of surgeons.Prior to surgery,some indices on the uninjured side were determined through the anteroposterior radiographs of pelvis using X-Caliper measurement tool,including rotation center of femoral head,rotation center of hip joint,collodiaphyseal angle,femoral transverse eccentricity,abduction moment arm,and moment arm of gravity.These indices were precisely calculated using trigonometric function.The angle of osteotomy plane was determined.Proper prosthesis was selected and eccentricity was adjusted.Following artificial hip placement,a comparison was made between uninjured and injured sides.MAIN OUTCOME MEASURES:Eccentricity and hip joint function.RESULTS:The eccentricity on uninjured side of 30 patients averaged 38.7 mm (range 22-57 mm) prior to surgery and that on the injured side averaged 39.2 mm (range 21-59 mm) after surgery.Limb discrepancy ranged 1.8-8.7 mm,with a mean of 3.4 mm,after surgery.There were 26 patients (86.7%) presenting long limbs and 4 patients (13.3%) showing short limbs after surgery.All patients were followed-up 0.5-2.5 years old.Harris hip score results were excellent in 10 patients,good in 19 patients,and fair in 1 patient,with an excellent and good rate of 97%.CONCLUSION:In treatment of femoral neck fracture,eccentricity adjustment during artificial hip replacement is closely associated with hip joint function after surgery.

In order to improve the reference service, the necessity to provide reference service for translational medicine in academic library was analyzed and the translational medicine-based reference service in Library of Qingdao University was elaborated from the aspects of its contents and ways with the existing problems and weaknesses summarized.

Objective To evaluate the multi-slice spiral CT scan of liver dynamic dual-phase three-dimensional vascular imaging portal phase clinical value. Methods 80 cases in clinic, who were patients with liver function and imaging diagno- sis of liver and portal hypertension in liver cirrhosis, and 20 cases of healthy persons were carried out multi-slice spiral CT dual-phase scanning. The workstation used volume rendering techniques (VR) and maximum density multi-planar recon- struction technique for reconstruction. Results The hepatic arterial phase VR image and MIP MPR images can clearly show the celiac trunk, splenic artery, hepatic artery or artery and its branches, including 2-3 grade tumor blood supply variation of blood vessels and blood vessels, the portal venous phase, VR images and MIP MPR images clearly show the 1-6 level structure and the portal vein and hepatic vein branches of 1-3, with strong three-dimensional sense of space. Conclusion The multi-slice spiral CT three-dimensional reconstruction of portal vein imaging is a fast and effective non-invasive an- giography techniques, contributing to the clinical choice of reasonable efficacy of treatment programs and follow-up.

Objective To monitor right heart hemodynamic changes of patients with lung cancer during and after the procedures of pneumonectomy and discuss the effect of pneumonectomy on right heart function and risk of postoperation morbidities. Method 16 patients with lung cancer were randomly selected and the clinical database was queried and Swan-Ganz catheter was applied awake from jugular vein to pulmonary artery ,measuring mean arterial pressure(MAP) ,heart rate(HR) ,central venous pressure(CVP) ,mean pulmonary artery pressure (MPAP),mean pulmonary capllary wedge pressure(MPCWP) ,and eardiaoc output(CO) and calculating cardiac index (CI),left ventricular-stroke work index (LVSWI), right ventricular stroke work index (RVSWI) , and stroke volume index(SVI) instantaneously before anaesthesia, after anaesthesia with single lung ventilation, after pulmonary artery and pulmonary vein occlusion and supine chest dosed during the procedure of pneumonectomy. Pre-and post-operative complications were recored. Results Cardiovascular complications occurred in 6 patients(37.5%) postoperatively. There were no operative or perioperative deaths. MPAP increased significantly after the procedure of pneumonectomy compared with that of preoperation, and MPAP > 26 mm Hg was in 4 patients who got cardiovascular complications postoperatively with morbidity of 100% ,which was "significantly higher than the morbidity of 16.7% (2/12) when MPAP < 25 mm Hg. Conclusion Pneumonectomy has significant effects on right heart hemodynemic changes and as MPAP increases postoperatively, the risk of cardiovascular complications becomes higher.

Objective:To observe the expression and correlation of eIF4E and c-myc in laryngeal squamous cell carcinoma.Methods:Western blot was applied to examine the expression of eIF4E and c-myc on tumor core,transition core and tumor-free core from 36 laryngeal carcinoma patients.The results were statistically analyzed.Results:The overexpression of eIF4E and c-myc protein levels suggests an increasing tendency from tumor-free core,transition core and tumor core;and shows an significant correlation of eIF4E and c-myc expression.Conclusion:The overexpression of eIF4E and c-myc cause the laryngeal carcinoma cell malignant transformation;the correlation of eIF4E and c-myc may provide a basis for gene therapy of laryngeal carcinoma.

Objective To observe the sensitivity ofastrocytoma to chemotherapeutic drugs, in order to explore the drug selection using chemosensitivity testing in vitro on astrocytoma. Comparing different WHO grade astrocytoma to chemotherapeutic drugs sensitivity differences,providing the basis data for clinical treatment. Methods The astrocytoma cells were seperated and cultured in vitro from 142 tumor tissues by glioma resection, including 55 cases of low-grade astrocytoma (WHO grade Ⅰ , Ⅱ) and 87 cases of high-grade astrocytoma (WHO grade Ⅲ, Ⅳ). The proliferation inhibition and chemosensitivity of tumor cells to 11 drugs were investigated by using MTT method. Results There were 54.93% (78/142) patients recommended chemotherapeutic drug in 142 cases. The percentage was 41.82%(23/55) in the low-grade astrocytoma, and 63.22% (55/87) in the high-grade astrocytoma respectively. Teniposide,carboplatin,vincristine as the sensitive drugs were similar in different WHO grade astrocytoma. Conclusions The chemosensitivity testing could be used to filtrate sensitive drugs to clinical chemotherapy. The action is more prominent in high-grade astrocytoma than in low-grade astrocytoma. There is no difference in the choice of sensible drugs among different WHO grade astrocytoma.

Adenoma, Oxyphilic ; classification ; pathology ; Carcinoma, Renal Cell ; classification ; metabolism ; pathology ; Humans ; Kidney ; pathology ; Kidney Neoplasms ; classification ; genetics ; pathology ; therapy ; Molecular Biology ; methods ; trends

Objective To investigate the relationship between promoter methylation of RASSF1A gene and laryngeal squamous cell carcinoma.Methods A methylation-specific PCR was performed to detect the promoter methylation of RASSF1A gene in 48 tumor tissues,48 corresponding normal tissues and 48 normal blood plasma.The expression of RASSF1A protein was determined by means of Western blotting.Results The methylation in RASSF1A gene was detected in 34(70.83%) tumor tissue samples,11(22.92%) corresponding normal tissue samples and 6(12.50%) blood plasma samples,respectively.The methylation degree of tumor tissue was higher than that of corresponding normal tissues and normal blood plasma(P