Objective:Toinvestigate the effect of ATP50 on GH-induced testosterone secretion in isolated SD rat Leydigcells.Methods:The Leydig cells were treated with ATP50 antisense oligodeoxynucletides(Anti-ODN)in GH group,nonsense tat ODN in control group,cAMP and testosterone in culture medium was detected by radioimmunoassay,expression of ATP50 protein was investigated by immunohistochemistry.Results:Antisense ATP50 ODN significantly inhibited GH-induced testosterone secretion of isolated rat Leydig cells in a dose-dependent manner(P

Objective To investigate the therapeutic effect and side effect of combined capecitabine with oxaliplatin on advanced and recurrent gastric cancer.Methods 56 patients with advanced and recurrent gastric cancer were treated with combined oxaliplatin and capecitabine chemotherapy,and the therapeutic effect and side effect were analyzed retrospectively.Results Among all 56 patients treated with combined oxaliplatin and capecitabine chemotherapy,4 patients (7.1%) were complete remission,26 patients (46.4 %)were partial remission,20 patients (35.7 %) were stabilization,6 patients (10.7 %) were progression,and the response rate was 53.6 %.The median remission time was 7.8 months,and the median survival time was 11.3 months.The main side effects were nausea and vomiting,diarrhea,hand-foot syndrome,peripheral nerve toxicity,transaminase increasing and leuco cytopenia,however,the side effects were mainly grade Ⅰ and Ⅱwhich could be tolerated by patients.Conclusion The combined oxaliplatin and capecitabine chemotherapy is an effective method and has better tolerance in treatment of advanced gastric cancer and is well worth clinical application.

Objective: To observe preventive and therapeutic effects of Ganlan Jiejiu Yin on acute alcoholic hepatic injury in the rat and the mouse, and to study preliminarily effects on alinine transaminase(ALT) and aspartic transaminase (AST) in serum and hepatic homogenate. Methods: The acute alcoholic hepatic injury model was made by stomach perfusion of white spirit for 5 days, and drunk time and the recovering time were observed every day, and serum ALT on the 3rd day and ALT and AST in serum and hepatic homogenate on the 5th day were determined, and the results were compared with those of Hugan Capsules. Results: Ganlan Jiejiu Yin could significantly decrease the drunk rate, increase the recovery/drunk ratio, and decrease ALT and AST in serum and ALT in hepatic homogenate with significant differences as compared with those of the control group. Conclusion: Ganlan Jiejiu Yin has good drunk-preventing, alcoholism-relieving and liver-protecting actions.

In view of the fact that the current concept of asymptomatic period of HIV infection can not express the disease development in this period accurately,and easily lead to ambiguity,the author proposed the concept of‘chronic progress phase’on basis of long-term clinical and scientific research.The author thought that the so-called asymptomatic period of HIV infection had many signs and symptoms of clinical significance,besides the syndrome can be identified,thus it was of great significance for the diagnosis and treatment of AIDS.The concept of‘chronic progress phase’being in accordance with the actual disease evolution,was a more scientific appellation and had been widely accepted by the industry.

Congenital cataract is the leading cause for low vision and blindness in infancy and childhood.One third of congenital cataract cases are associated with genetic mutation and hereditary,and the etiology of congenital cataract is heterogenous and its phenotype is variable.The known mutation genes include encoding structural lens protein,gap junction protein,membrane protein and lens-developing-related regulatory protein.Location and identification of mutation genes in congenital cataract patients are necessary for us to understand the molecular defects and pathophysiologic features of congenital cataract.With the development of molecular biology techniques,the study on the mechanism of congenital cataract has made great progress,which is helpful for us to further understand the heredity pattern as well as the influence of environment and nourishment to the metabolism of lens.The purpose of this review was to summarize the literature of current advance in the study on molecular genetic basis of congenital cataract.

This article proposed a new cognition on the duration of asymptomatic HIV infective stage.It expounded the reason for treating AIDS with TCM first choosing asymptomatic HIV infective stage from the views of "preventive treatment of disease" and treatment based on syndrome differentiation.

Objective: To summarize our experience on retroperitoneal laparoscopic radical nephrectomy (RLRN) in treatment of patients with renal carcinoma. Methods: The clinical data of 180 patients with renal carcinoma, who received RLRN from Jan. 2001 to Jan. 2007, were retrospectively studied. The experience was summarized and the clinical outcomes of patients were assessed. Results: All the 180 patients were pathologically confirmed to have T1-T2 phase renal carcinomas, with 87 cases had left side lesions and 93 had right ones. The diameter of tumors was 1.5-8 cm, with a mean of (4.8±1.3)cm. All the patients were subjected to RLRN and all underwent successful operation. The operation time was 56-90 min, with a mean of (70±12) min; the blood loss was about 70-1 500 ml, with a mean of (122±36) ml. One patients had right renal venous injury (not converted to open surgery), 7 patients had pleural injury, 1 had diaphragmatic injury, 2 had subcutaneous emphysema, 1 had incision hernia, and 6 had delayed union of incision. The patients began oral intake of food 24-48 h after operation and were discharged 7-9 days after operation. Forty-one patients were lost during the follow up (4 months to 6 years); 4 patients died due to other reasons and 2 had pulmonary metastasis. There was no puncture site metastasis in this group. Conclusion: RLRN has the advantages of less trauma, rapid recovery, and slight pain. It is a safe and effective method for treatment of renal carcinoma.

Objective: To compare the clinical outcomes of laparoscopic nephrectomy and open nephrectomy for radical treatment of renal cancer. Methods: Fifty-three patients with renal cell carcinoma (due to receive radical renal tumor resection) were randomly allocated into 2 groups: retroperitoneal laparocopic (RL) group (n = 27) and open approach (OA) group (n = 26). The operation time, hospital stay, use of painkiller, fasting period after operation, and blood loss during operation were analyzed and compared. All the cases were followed up for 6-12 months and the survival rates, wound healing, and carcinoma metastasis were recorded. Results: Patients in both groups were all successfully treated and their sexes, clinical stages, ages, and tumor sizes were all comparable. The operation time was similar in the 2 groups, with that of RL group being (66.66±10.37) min and of OA group being (69.08±11.22) min. The fasting period in RL group ([1±0.36] d) was significantly shorter than that in the OA group ([2±0.68] d, P<0.05). The hospital stay in RL group (6.92±0.96) was significantly shorter than that in OA group (11.42±1.57, P<0.05). The blood loss in RL group ([72.03±19.37] ml) was less than that in OA group ([154.4±20.42] ml, P<0.01). The use of painkiller was 2 person-times in the RL group and 20 person-times in the OA group (P<0.01). No post-operation infection was noticed in RL group and there was one case of infection in OA group during follow-up. Conclusion: Compared with traditional open radical nephrectomy, laparoscopic radical nephrectomy has the advantages of rapid recovery, less pain, less blood loss, and less complications, etc.

Carcinoma, Squamous Cell ; diagnosis ; Humans ; Maxilla ; pathology ; Maxillary Neoplasms ; diagnosis ; Neoplasm Metastasis ; Spinal Neoplasms ; secondary

The determination and characterization of solid drug form polymorphisms plays an important role in drug quality control, selection of the production process and clinical efficacy evaluation. Vibrational spectroscopy is a powerful method for the characterization of drug polymorphisms. In this paper we review recent research and application advances in the polymorphic characterization of active pharmaceutical ingredients (APIs) and drug cocrystals/salts by using Fourier transform infrared (FTIR) and Raman spectroscopy to elucidate the characteristics of APIs and drug complexes. This may provide theoretical support for structural analysis during the development process for drugs.