OBJECTIVETo prove if it is possible for using the shattering extraction with solvent to extract ingredients of traditional Chinese medicine.

METHODThe shattering extraction with solvent, the refluxing extraction and the ultrasonic extraction were used to extract paeoniflorin from Radix Paeoniae rubra, and to extract baicalein from Radix Scutellariae, and to extract chlorogenic acid from Flos lonicerae japonicae respectively, using ingredient content and extract yield as the measuring indexes.

RESULTThe content of each every ingredient obviously higher by using shattering extraction with solvent than using refluxing extraction or the ultrasonic extraction.

CONCLUSIONThe shattering extraction with solvent is a high efficiency, simple and quick extraction. It may be used to extract the ingredient of three kinds of traditional Chinese medicine.

Benzoates ; isolation & purification ; Bridged-Ring Compounds ; isolation & purification ; Chemical Fractionation ; methods ; Chlorogenic Acid ; isolation & purification ; Drugs, Chinese Herbal ; chemistry ; Flavanones ; isolation & purification ; Glucosides ; isolation & purification ; Monoterpenes ; Solvents ; chemistry ; Time Factors

OBJECTIVETo explore the anti-inflammatory effect and possible mechanism of Shuang-huang-lian (SHL) microemulsion.

METHODRat model of pleuritis was established by thoracic injecting 0.2 mL of 1% carrageenan. Rats in the treated groups were orally administered with SHL microemulsion prescription 1, 2, and oral liquid, while those in the positive control group were given aspirin. Rats in the normal group and the model group were given equal volume of water. Each groups were given their medicine for successive 6 days. Modeling was performed 30 mins after the 5th day medication. After 12 hrs of modeling, took suction of the pleurorrhea and measured the amount of tumor necrosis factor alpha (TNF-alpha), interleukin-8 (IL-8), prostaglandin E2 (PGE2) and protein (pro).

RESULTCompared with the normal group, all the parameters were higher in model group (TNF-alpha and IL-8 P<0.01, PGE2 and pro P<0.05). While compared with the model group, only the amount of TNF-alpha and PGE2 were lower in all the treated group (P<0.01).

CONCLUSIONBoth SHL microemulsion prescription 1 and 2 have obvious anti-inflammatory effect. The effect might be related to inhibiting the increase of cytokines as TNF-alpha and PGE2, and intervening of the metabolic process of arachidonic acid (AA).

Animals ; Antineoplastic Agents ; pharmacology ; therapeutic use ; Carrageenan ; adverse effects ; Cytokines ; metabolism ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Emulsions ; Male ; Pleurisy ; chemically induced ; drug therapy ; metabolism ; Rats ; Rats, Wistar