As the reference method, microscopic examination is still the most basic method of red cell morphology examination.There are several ways to improve the laboratory personnel's skill and ability on abnormal erythrocyte morphology diagnosis, including deep understanding of pathological mechanisms of abnormal red cell morphology, raising the ability of recognizing abnormal red blood cell that are of great clinical value, following and implementing rules to ensure the accuracy and repeatability of the examinations, standardizing reports of abnormal erythrocyte morphology.

Objective To investigate the efficacy of normative ankle balance training on chronic ankle instability (CAI).Methods Ninety patients were randomized into control group (17 males,28 females;13-26 years,mean 19.6 years) and experiment group (18 males,27 females;14-27 years,mean 20.3 years) according to the random number table.There were 19 left ankles and 26 right ankles injured in control group,and 19 left ankles and 26 right ankles injured in experiment group.Patients in control group only completed routine muscle strength training.Besides the routine muscle strength training,balance training (rubber band,spongy cushion,balance pedal,rubber balance ball and jump ball) were added in experiment group.The two groups were compared with respect to foot and ankle disability index (FADI),anterior,posterior-median and 45° to posterolateral direction reach distance of star excursion balance test (SEBT) and visual analogue score (VAS).Results After training,comparison of experiment and control groups showed significant differences in FADI (35.16 ± 3.23 and 22.33 ± 1.36),SEBT distance in posterior median direction [(78.59 ± 7.22) ％ and (71.89 ±7.11)％],SEBT distance in 45° to posterolateral direction [(75.79 ± 8.27)％ and (68.95 ±8.03) ％] and VAS [(3.01 ± 0.91) scores and (3.42 ± 1.02) scores] (P ＜ 0.05).For intragroup comparison,FADI,SEBT and VAS in experimental group showed significant differences before and after training (P ＜ 0.05),but only FADI in control group differed significantly before and after training (P ＜0.05).Conclusion Normative balance training can improve CAI patients' self-feelings of dynamic balance and instability with satisfactory therapeutic efficacy,and is worthy to be popularized.

(Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.hours to induce RPE cells apoptosis.The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR).Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular Ca2+ fluorescence imaging system.RPE cells and it significantly increased after co-cultured with Ver.The fluorescence in resting RPE cells was strong and distributed throughout the cells.The nucleus appeared more fluorescent than the cytoplasm.Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.[Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.

Objective To investigate the size distribution and change of plasma DNA in different gestational weeks between the normal and abnormal pregnancy.Methods Two methods were applied to measuring the lengths of different derived plasma DNA fragments.For the maternal derived DNA,GAPDH gene distribution of each DNA in size-fractionated fragment was assayed by real-time PCR after gel electrophoresis separation;for the fetal derived DNA,size distribution of SRY gene of fetal derived DNA was also determined by using a series of real-time PCR.Results Compared with that of matenal derived DNA,the fragment length of fetal derived DNA was mostly shorter than 300 bp.Compared with that in mid-pregnancy,plasma maternal derived DNA fragment distribution had no significant difference in late-pregnancy and in patients with high-risk of carrying a Down's child,whereas fetal derived DNA fragment was a bit longer.Conclusion The fragment length of fetal derived DNA is significantly longer than that of fetal one in maternal blood.Moreover,because of abnormal pregnancy and accompanied with the pregnancy progress,the number and physico-chemical property(fragment length,for example) of fetal derived DNA has a certain change.

Objective To observe and analyze the oral and maxillofacial appearance of patients with acquired immune deficiency syndrome(AIDS)and to explore the effective detection methods of hunman immunodeficiency virus(HIV)infection,aimed at early diagnosis of HIV infection and AIDS,providing reference for treatment and prevention.Methods 21 6 cases of suspected HIV infec-tion and AIDS therapy-naive patients in the department of infection disease in this hospital,from January 2012 to March 2014,were selected.Oral and maxillofacial appearances,detection methods and its results were retrospectively analysed.Results The main clinical manifestations of patients with HIV infection and AIDS were oral candidiasis,oral hairy leukoplakia,herpes simplex virus infection,Kaposi′s sarcoma,periodontal disease,ulcer disease and other abnormal attribute.All of the 21 6 samples were positive in the preliminary screening,214 cases were confirmed by western blotting(WB),and the sensitivity,specificity and false positive rate were 99.07%,100.00% and 0.926%,repectively.Conclusion Focusing on clinical symptoms and signs in oral and maxillofacial re-gion of patients with HIV infection and AIDS could provide significant references for clinical diagnosis,and combing detection meth-ods of enzyme linked immunosorbent assay(ELISA)and WB could make a definite diagnosis,which might be benefit to make effec-tive prevention and treatment measures and in order to control the infection and improve the prognosis.

Objective To explore the diagnosis and treatment rule of eczema by Zhuang Guokang. Methods Data of 58 eczema patients treated by Zhuang Guokang were collected as research objects to set up the database. Support vector machine (SVM) method was adopted to take data mining research of the key points of syndrome differentiation and the rule of medication. Results The nourishing Yin method was extracted as the category property to get 3 classification models of the syndrome differentiation key points, the related treatment methods and medication rule and 63 relevant quantitative data. The accuracy rate of model classification of the main differentiation points of nourishing Yin method corresponding to Yin reached 77.586%, and Kappa value was 0.536. Among these points, the weight of chapping(1.558), papules(1.456), thready pulse(1.036)and desquamation(0.858) got higher significance. The accuracy rate of model classification of medication feature reached 86.2069%, and Kappa value was 0.719. Among these medicines, Pseudostellaria heterophylla(0.776), Semen plantaginis (0.550), Asparagus root(0.525) and Solomonseal (0.388) got higher significance. Conclusion The research revealed the syndrome differentiation key points and the medication rule of Zhuang Guokang’snourishing Yin methodin the treatment of eczema, and explored the path of experience transforming to knowledge of the famous old TCM doctors.

Objective A meta-analysis was conducted to figure out the effects of anesthetic methods on postoperative myocardial infarction in noncardiac surgery patients.Methods We searched PubMed, Embase, Ovid, Cochrane Library, Google scholar, CNKI, Wang-fang data and VIP Database (by September 2016) to identify relevant studies that focused on the effect of intrathecal blockade on postoperative myocardial infarction and mortality in non-cardiac surgery.Meta-analysis was performed using software of RevMan 5.3.Results A total of 21 859 patients from 10 RCTs were enrolled.The meta-analysis showed that there were no differences in myocardial infarction within 7 postoperative days (OR=0.44, 95%CI 0.13-1.46, P=0.18) or 30 days (OR=1.49, 95%CI 0.89-2.49, P=0.13) and all-cause mortality (OR=1.26, 95%CI 0.84-1.88, P=0.26) between epidural anesthesia combined with general anesthesia and general anesthesia.Furthermore, there were no differences in myocardial infarction within postoperative 7 days (OR=1.14, 95%CI 0.31-4.17, P=0.84) and all-cause mortality within postoperative 30 days (OR=0.88, 95%CI 0.43-1.79, P=0.73) between spinal anesthesia alone and general anesthesia alone.Conclusion Intrathecal blockade cannot affect the incidence of myocardial infarction and mortality in high-risk cardiac patients undergoing intermediate or high-risk non-cardiac surgery.

Objective To evaluate the clinical efficacy and complication rate by open or retroperitoneal laparoscopic radical nephrectomy for renal tumor with stages T1N0M0 or T2N0M0.Methods Between October 2003 and October 2006,90 patients with renal cell carcinoma,which were clinically localized stages T1N0M0 or T2NOM0 Based on the patients' options to undergo retroperitoneal laparoscopic radical nephrectomy (group A,49 patients)and open radical nephrectomy(group B,41 patients).The clinical efficacy were compared between group A and group B,retrospectively.Results In group A,the operations of 46 patients were successful,4 cases occurred major complications(8.7%)during the follow-up visit which lasted for (21.9±6.1)months.The operations in group B were all successful.9 cases occurred major complications (22.5%)during the follow-up visit which lasted for(24.9±7.8)months.All cases were renal malignant tumors with pathologically confirmed stages T1N0M0 or T2N0M0 and there were no renal pedical lymph node metastasis.The age,weight,body mass index(BMI),tumor size,operating time and the time of follow-up were no statistically significant differences between the two groups(P＞0.05),while the blood loss,amount of postoperative drainage,time to ambulation,recovery of intestinal function after operation,hospital stay,use of analgesic and transfusion blood or plasma in group A were significantly reduced than those in group B(P＜0.01). Conclusions As compared with open radical nephrectomy,retroperitoneal laparoscopic radical nephrectomy afords patients with renal cell carcinoma an impreved postoperative course with minimal invasion,less pain,quicker recovery and less complications,while providing equally effective cancer control for patients with T1N0M0 or T2N0M0 tumor.

Objective To explore a new method of comparing the references images first to enhance the precision of the central point of the radiation treatment planning(RTP), try to establish a reference standard for this method in the nasopharyngeal cancer(NPC)and carcinoma of utercin cervix in the work of position verification. Methods For 50 RTPs of NPC and 20 RTPs of carcinoma of utercin cervix, the reference-CT-images in set-up and in position verification were compared, and to measure the difference between the two images. Then, in the same way, compare and measure the difference in the central-pointimages. Results For NPC, there was over 90% RTPs in which every difference measured was less than 2 mm;for carcinoma of utercin cervix, over 80% RTPs meet the criterion:the value of △MU1 ' or △MU2' was less than 5 mm and the others are less than 3 mm. Conclusions By comparing the references-CT-images in set-up and in position verification, the precision of the central point of the RTP is enhanced. The marks on the skin become more credible. So, it is feasible to perform the criterions in the work of position verification:for NPC every difference measured is less than 2 mm;for carcinoma of utercin cervix the value of △MU1 ' or △MU2 ' is less than 5 mm and the others are less than 3 mm.

Objective To explore the attentional biases in individuals with different types of obsessivecompulsive symptom and the correlations between the attentional bias of high obsessive compulsive symptom (HOC) and symptoms. Methods 22 individuals with HOC and 38 individuals with low obsessive compulsive (LOC) symptom completed the Chinese Emotional Stroop task that assessed the attentional bias. Comparisons were made between HOC and LOC and in different types of HOC, and the correlations between the attentional bias of HOC and symptom severity scores were searched for. Results The comparison between HOC and LOC on the reaction time of neutral, negative and disgust-related words were not significant. The contamination/washing subtype in HOC showed faster reaction time on negative and disgust-related words compared to that of neutral words,and its attentional bias exhibited significant correlation with symptom severity scores( r= -0. 648, P=0. 031 ). Conclusion Such information point out the contamination/washing subtype may have a different neural mechanism compared to the other subtypes of OCD.