Objective To compare the effect of hepatic oval cells and bone marrow mesenchymal stem cells to treat liver fibrosis in mice. Methods The C57BL/6 mice were fed with 3,5-diethoxycarbonyl-1, 4-dihydro-collidine (DDC) in a standard food at a concentra-tion of 0.1% to activate the oval cells proliferation, then the nonparenchymal cells were separated by in situ perfusion and density gradient centrifugation, and finally Sca-1 antibody was used in conjunction with magnetic activated cell sorting (MACS) to separate the Sca-1 +cells. Other mice were injected subcutaneously with CCl4 to establish experimental liver fibrosis model. Finally, the fibrosis mice were trans-planted with HOC or MSC or saline by spleen injection according to the group they belonged to. Four weeks later, the liver function index, the hydroxyproline and the histopathological change among the groups were used to evaluate the effect of the treatment. Result The liver function index was improved after transplantation. HOC and MSE treatment could reduce both the hydroxyproline level and alleviate the fibro-sis, but HOC seems more effective in the group with continuing CCl4 injection(P < 0.05). When stopping injecting CCl4, the change of fi-brosis was not visible(P >0.05). Conclusion Both hepatic oval cells and bone marrow mesenchymal stem cells can improve the liver function, and alleviate the fibrosis level by transpleen transplantation, but hepatic oval cells were more effective.

especially in the group of ceasing CCl4 injection. Conclusion After transplanted into the liver by spleen injection, hepatic oval cells could plant in the portal area, which improve liver function and alleviate the cirrhosis level.

Objective To Study the effect of splenectomy plus endoscopic varices ligation (SEVL) in the treatment of portal hypertension. Methods SEVL was carried out in 38 patients with portal hypertension from January 2000 to June 2002. The authors compared hemodynamic parameters and gastric emptying time before and after the operation. The incidence of upper gastrointestinal bleeding, the disappearance rate of esophageal varicosis, the outcomes of portal hypertensive gastropathy (PHG) and the incidence of complications were recorded at postoperative 3 weeks, 6 months, 12 and 18 months, respectively. Results Postoperatively, the free portal pressure, portal flow volume and velocity were significantly reduced in all the cases ( P 0.05). Portal thrombosis was seen in 6 cases (6/38, 15.8%). Conclusions SEVL has little influence on gastric emptying and PHG, offering a high disappearance rate of varicosis and a low incidence of complications.

Medical students should study and enlarge their medical terminology from their medical English courses as well as bilingual courses. This ability is a key part in the cultivation of high-levelled medical talents.However,medical words are special from general English in three parts:long,great in numbers and difficult to read.These characteristics leave the medical students in a hard situation in their promotion of medical terminology.If the students are provided and guided with a convenient and efficient software based on modem education technology,they can achieve much better results.The audio medical English terminology corpus can help the medical students achieve their goal in promotion of medical termi-nology.

Objective To investigate the alteration in gene expressions of collagen type Ⅰ and type Ⅲ in tissue of obstructive low compliant bladder of rats. Methods Gene expressions of collagen type Ⅰ and type Ⅲ in the rat bladder tissues were evaluated with RT-PCR. Results The gene expressions of collagen type Ⅰ and type Ⅲ in smooth muscle of low compliant bladder were up regulated, but the level of type Ⅲ gene expression was stronger than that of the type I. Conclusion Collagen type Ⅲ and Ⅰ gene expression was upregulated in tissue of obstructive low compliant bladder of rats. Up regulation of collagen gene expression was related to low compliant bladder after obstruction.

Objective:To explore whether the mRNA expression of TNFRSF12 in peripheral blood leukocytes of SLE patients is abnormal and to determine whether the TNFRSF12 gene is susceptibility gene to SLE.Methods:The level of mRNA expression of TNFRSF12 in peripheral blood leukocytes was detected by TaqMan one-step RT-PCR method.Results:The mRNA expression pattern in SLE patients is different to that of normal control.The mRNA in SLE consists of 2 types of isoforms of TNFRSF12,encoding membrane bound glycoprotein and potentially secreted molecules respectively,whereas only the latter in normal control.After PHA treatment,the mRNA expression pattern in normal control becomes the same as that of SLE patients.Overall TNFRSF12 mRNA expression was significantly decreased in active SLE group and significantly increased in lupus nephritis group.Conclusion:The results indicated that TNFRSF12 may play a role in the pathogenesis of SLE and was susceptibility gene to SLE.

Objective To evaluate the effect of endoscopic variceal ligation(EVL) combined with splenectomy for the patients with portal hypertension. Methods Thirty two cases of portal hypertension underwent EVL plus splenectomy from May 1999 to May 2002 in our department. The patients were registered and followed up in the third, sixth and twelfth months after operation respectively. Re ligations were performed in case of relapsed varices. Results Thirty one patients were followed up for ≥2 years.Of them,esophageal varices recrrented in 6.4% of patients during following-up period,but no reblecding, and no death caused by hepatic encehpalopathy or other complications.Conclusions EVL combined with splenectomy has less trauma , less postoperative complications and high eradication rate of esophageal varices, so it can be used safely in the treatment of patients with ruptured esophageal varices.

Objective:To study the significance of QT dispersion(QTd) changes in electrocardiogram(ECG) after coronary artery bypass graft. Methods:Forty eight patients scheduled for elective coronary artery bypass graft were studied. The changes of QTd were monitored by 12 lead electrocardiogram system before surgery (as baseline values), at the end of surgery, and 1 4 d and 7 d after surgery.Results:The baseline values for QTd was (56.32?2.25) ms.QTd were significantly longer as compared to the baseline values ( P

Objective : To understand the current situation and influencing factors of milk and dairy intake among primary and middle school students in Tongzhou District, Beijing, so as to provide the reference for the intervention of milk and dairy intake among primary and middle school students. Methods: The stratified cluster sampling method was used to randomly select the students from the third to sixth grades of primary school, three grades of junior middle school and three grades of senior high school from downtown and township of Tongzhou District as the survey objects. The demographic information, family data, and milk and dairy intake of a week before were collected. Taking the average daily intake of 300 g milk and dairy as the standard (the Chinese Dietary Guidelines 2016), the multivariate logistic regression model was used to analyze the influencing factors for reaching the standard of average daily milk and dairy intake among the students. Results: A total of 804 questionnaires were sent out, and 771 valid questionnaires were received, with a recovery rate of 95.90%. There were 321 primary school students, accounting for 41.63%; 228 junior high school students, accounting for 29.57%; and 222 high school students, accounting for 28.80%. The intake rate of milk and dairy products was 90.92%, and the adverse reaction rate was 10.12%. The daily intake rate was 36.71%. The median daily intake was 214.29 g, and the rate of average daily intake reaching the standard was 28.02%. The multivariate logistic regression results showed that primary school students (OR=1.672, 95%CI: 1.102-2.535), middle school students (OR=2.086, 95%CI: 1.349-3.225), overweight (OR=1.747, 95%CI: 1.131-2.700), obesity (OR=2.469, 95%CI: 1.698-3.591), and parents with bachelor's degree or above (OR=1.760, 95%CI: 1.022-3.029) were the influencing factors for reaching the standard of average daily milk and dairy intake among the students. Conclusions The average daily intake of milk and dairy products of primary and middle school students in Tongzhou District does not reach the recommended standard, and grade, body mass index and parents'education level were the influencing factors.

Animals ; Cardiomyopathy, Dilated ; Disease Models, Animal ; Mice ; Mice, Transgenic