AIM: To study the effects of angiotensin Ⅳ(Ang Ⅳ) on growth of cultured neonatal rat cardiomyocytes.METHODS: Cardiomyocytes isolated from neonatal SD rat were cultured in vitro and divided into groups: control DMEM,10~(-6) mol/L Ang Ⅳ, 10~(-5) mol/L Ang Ⅳ.The effects of Ang Ⅳ were assayed as follows: protein synthesis was determined by modified Bradford method,and cell cycle by flow cytometry.RESULTS: Ang Ⅳ promoted protein synthesis(P

Appendicitis ; complications ; Humans ; Infant, Newborn ; Intussusception ; complications

Colectomy ; methods ; Colorectal Neoplasms ; mortality ; pathology ; surgery ; Humans ; Laparoscopy ; Treatment Outcome

Fistula ; complications ; surgery ; Humans ; Male ; Maxilla ; pathology ; Middle Aged ; Osteomyelitis ; complications ; surgery

Medical students should study and enlarge their medical terminology from their medical English courses as well as bilingual courses. This ability is a key part in the cultivation of high-levelled medical talents.However,medical words are special from general English in three parts:long,great in numbers and difficult to read.These characteristics leave the medical students in a hard situation in their promotion of medical terminology.If the students are provided and guided with a convenient and efficient software based on modem education technology,they can achieve much better results.The audio medical English terminology corpus can help the medical students achieve their goal in promotion of medical termi-nology.

Anesthesia related skills training is an important component of standardized residency training plan.Attention should be paid to the clinical thinking ability,preoperative assessment capacity,sterile concepts and technologies,cardiopulmonary brain recovery skills and teamwork ability cultivation during the rotation of resident physieians and resident surgeons,and comprehensive and scientific examination system should be established

Objective: To analyze the influence of long-term swimming training on spatial learning-memory in rats and its relationship with cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP) signal transduction pathway. Method: After 3 times adaptable swimming exercises (30min each time), 40 male SD rats were divided into 2 groups: control group (CR, n=20) and exercises, group (TR, n=20). CR group didn't swim, and TR group swam without burden (6 times/week, 60 min each time). After 8 weeks training, 10 rats were selected from both groups respectively for examing of Morris water maze test. Radioimmunoassay was used to measure the levels of cAMP and cGMP in hippocampus and prefrontal cortex of rats. Result: ①Compared with CR group, in TR group learning-memory improved in a certain extent: ②Compared with CR group, in TR group, the level of cAMP in hippocampus enhanced very obviously (P＜0.01), the cAMP/cGMP ratio enhanced obviously (P＜0.05); in prefrontal cortex the levels of cAMP and cAMP/cGMP ratio enhanced obviously (P＜0.05). Conclusion: Swimming training for 8 weeks improved the spatial learning-memory of rats in a certain extent, and elevated the level of cAMP and cAMP/cGMP ratio in hippocampus and prefrontal cortex of rats obviously. It is suggested that exercises might affect learning-memory by cAMP and cGMP signal transduction pathway.

Ear Diseases ; congenital ; surgery ; therapy ; Ear, External ; abnormalities ; Ear, Middle ; abnormalities ; Humans

Objective:To compare the effects of levosimendan injection at single dose and multiple doses on the left ventricular function , biomarkers and neuroho-rmonal activity in the patients with acute exacerbation of advanced heart failure .Methods:Totally 39 patients with chronic heart failure at acute exacerbation were divided into two groups , single dose group and multiple doses group .All the patients were given loading dose of 6μg· kg-1 levosimendan at baseline , and then given 0.1μg· kg-1 · min-1 continuous 24h in-fusion pump.The above regimen was administered once again after one month and six months in the patients of multiple doses group . Left ventricular end systolic volume ( LVESV ) , left ventricular end diastolic volume ( LVEDV ) , left ventricular ejection fraction (LVEF), mitral annulus systolic velocity (SM), myocardial performance index (MOI), blood brain natriuretic peptide (NT-proB-NP), tumor necrosis factor alpha (TNF-α) and interleukin 6(IL-6) were compared between the two groups before the treatment , and after tge three-day and 6-month treatment.Results:Compared with those before the treatment , LVESF, LVEF, Sm, MPI, NT-proB-NP, IL-6 and TNF-αin in the two groups were significantly improved after the three-day treatment (P<0.05).The indices were sig-nificantly improved in multiple doses group after the 6-month treatment when compared with those before the treatment and in single dose group, and the differences were statistically significant (P<0.05).Conclusion: Repeated intravenous administration of levosi-mendan is superior to the single dose administration in improving hemodynamics and inflammatory factors .

Objective To study the relationship between ambulatory arterial stiffness index and extent of coronary atherosclerosis.Methods All subjects were underwent coronary CT angiography.Ambulatory arterial stiffness index(AASI) was measured before coronary CT angiography.AASI value between different periods in each group were calculated and compared.Results AASI between different periods in the normal control group and coronary heart disease group existed statistical difference (P ＜ 0.05).The extent of coronary sclerosis and AASI between different periods in different coronary artery lesions groups were statistically different(P ＜0.05),AASI was proportional to the degree of coronary sclerosis,24 h AASI,DAASI,NAA SI were related to extent of coronary atherosclerosis lesions,the correlation coefficient between NAASI and extent of coronary atherosclerosis was the largest.Conclusion AASI night is related to coronary atherosclerosis most closely,namely the higher the value of the nighttime AASI,the greater the degree of coronary atherosclerosis.