ObjectiveTo study the correlation between blast lung injury (BLI) and acute posttraumatic stress disorder(PTSD) through the establishment of the model about blasting injury of rats.Methods84 SD male rats were randomly divided into four groups:the normal control group( A),the model groups ( B,C) and the sham model group(D).21 rats in each group.The chest of the model group and sham model group rats,respectively,were exposed to 8＃ instantaneous electric detonator at different distances.Then,emotion and behavior of rats was observed through open field test and elevated plus maze test,at day 1,14,28 after blasting.Group A was not given the explosion of stress treatment.After testing,each group was randomly picked up seven rats for succumbing to draw blood which was used to detecting the level of plasma corticosterone by radioimmunoassay,respectively.Results( 1 ) On day 1,it was a significant difference that the latency time ( LT),crossing frequency (CF),rearing frequency(RF) and modification frequency(MF) which were both group B( (25.34 ± 4.50)s,( 19.73 ± 11.10 ),(0.52±0.11),(0.53±0.21) respectively)and group C((22.13 ±6.54)s,(28.67 ±7.22),(1.07 ±0.26),( 0.89 ± 0.15 ) respectively ) were compared with group A,including LT,CF and RF of group D ( ( 23.71 ± 3.9 ) s,(25.13 ± 12.35 ),( 1.26 ± 0.74 ) respectively ) (P ＜ 0.05 ).Then,on day 28,although there was a significant difference which both the LT,CF and RF of group B,and the CF and RF of group C were compared with group A(P ＜ 0.05 ),no significant discrepancy was observed between group B and C (P ＞ 0.05 ).( 2 ) In the elevated plus maze test,the result was similar to the open field test.(3)On day 1 after blasting injury,it was significant distinction that the level of serum corticosterone of group B,C and D (( 153.15 ± 44.50) ng/ml,( 132.72 ±78.77)ng/ml,(96.16 ±8.96)ng/ml respectively)were higher than group A(P＜0.05).Finally,the level of serum corticosterone of group B,C and D were lower than group A on day 28 (P ＜ 0.05 ).ConclusionBLI model successfully induces acute stress disorder(ASD) of rots which behaved like anxiety,fear,hyperarousal and avoidance,and promots furtherly the development of ASD to PTSD,and establishes the modle of acute PTSD.

To explore the effect of comprehensive management on psychological influence in children with asthma, 60 cases with asthma was administrated by WHO global comprehensive strategy for the management of asthma including the persistent use of beclomethaone with the intermittent inhalation of salbutanmol, the lung function monitored by peak flow meter, the detection and avoidance of the induced factors, the management of the social education and the psychological intervention. All children on comprehensive management were followed one year for observation. The results showed that the rate of the total effect was 98.3%. There were all decrease significantly in the rate of the hospitalization, the rate of the emergency, and the rate of the absence for school. It was obvious that asthmatic children and their parents's confidence for the treatment of asthma enhanced significantly.Their psychological disturbance, the school grade, and the life quality were also obtained advanced improvement.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

We compared the total errors observed from both precision and accuracy validation experiments with defined medically allowable errors for each quantitative test,and used Westgard's method evaluation decision chart for initial judgement of the acceptability of method validation performance. Moreover,we need to judge the acceptability of other method validation parameters,so that we could integratedly assess and judge whether the testing system is acceptable to use in the laboratory.

Objective To understand the actual psychological conditions of pregnant patients with gynecologic malignancies during chemotherapy so as to instruct the nurses to provide individualized psychological care to the patients and their relatives. Method The in-depth interviews were conducted to 7 patients by implementing the phenomenological approach of qualitative study. The acquired data were analyzed. Result The patients experienced four psychological conditions: pessimism about prognosis, anxiety to know more about the chemotherapy and guilty to their family and hope for treatment effect. Conclusion Nurses should pay attention to the psychological conditions of pregnant patients with gynecologic malignancies and help them successfully live through the treatment.

Objective To assess the removal efficiency of bacteria by water purifiers using different purifying materials. Methods The bacterial removal effectiveness of 7 types of commercially available water purifiers was tested using the National Standard Methods. Results Apart from one type using a single activated-carbon filtering material, the bacteria removal effectiveness of the other 6 types of water purifiers met the requirements of water purification device according to the rated treated-water-capacity conditions. Conclusion The removal efficiency of bacteria by water purifiers made from different filtering materials was different. Bacteria elimination effectiveness of a single-filtering material was lower and it is inversely proportional to its water-treating capacity. Water purifiers using multiple-purifying materials gave better removal efficiency of bacteria.

Objective To investigate the etiology and management of early postoperative hyperbilirubinemia after liver transplantation. Methods The etiology and dynamic alteration of early postoperative hyperbilirubinemia in 50 liver transplants were retrospectively analyzed by a comparative trial of clinical manifestation with serial liver biopsy. Results The total serum bilirubin (TB) level profile presented like a invert "S" curve. At the first week, second week and 4th week after liver transplantation the serum TB levels were in average ( 127.19? 113.15)? ( 135.45? 124.6) and ( 73.1? 49.52)??mol/L respectively. Three months later, the serum TB level approximated to normal TB level ( 29.8? 37.56)??mol/L. The dynamic alternations of total serum bilirubin level were incorporated with the morphological improvement under microscopy of liver allograft following liver transplantation. The initial hyperbilirubinemia of reciepient before liver transplantation (10 cases, 20?%), preservation injury (containing 44 cases of ischemic reperfusion injury, 88?%), acute cellular rejection (13 cases, 26?%) and bile duct leakage (4 cases, 8?%) were 4 essential causes responsible for the early postoperative hyperbilirubinemia. The total serum bilirubin level profile was not characteristic of each catergory. Those 4 casuses mentioned above presented either independently or concomitantly in concrete case. No primary hepatic failure (PHF) occurred and curability of hyperbilirubinemia was about 100?% in our series. Furthermore, the perioperative survival rate of the recipients and liver allograft was 90.6?% and 1-year accumulative survival rate was about 80?%.Conclusions The hyperbilirubinemia is common clinical manifestion within 3 months after liver transplantation. Preservative injury, acute rejection, preoperative hyperbilirubinemina and bile duct leakage are four essential causes. The comprehensive management targeted to etiology can usually achieve a good outcome for the reciepients with hyperbilirubinemia.

Objective To study the relationship between IP 3 and detrusor cell contraction mediated by M 3R. Methods [3H]-IP contents of human cultured detrusor cells were detected after stimulated by carbachol,atropine,methoctramine and 4-DAMP respectively. Results [3H]-IP contents increased with carbachol concentration.On the different concentrations (10 -9、10 -8、10 -7、10 -6、10 -5、10 -4 mmol/L )of 4-DAMP,[3H]-IP contents were 3 926.57?273.29、2 780.52?211.09、2436.84?153.62、1 973.22?164.71、1 372.38?141.35 and 1 107.98?920.45 cpm respectively.On the some concertrations of Atropine,[3H]-IP contents were 3 602.69?280.17,2 891.31?207.45,1 983.97?145.74,1 269.57? 105.31,1 106.37?75.23,927.50?77.36/min,respectively.On the same concentrations of methoctramine, the [3H]-IP contents was 4 462.74?360.69、3 938.61?327.13、3 315.45?270.36、3 063.19? 246.79、2927.37?226.45 and 2 836.55?241.63 cpm.This donoted that 4-DAMP and atropine signficantly inhibited the effect of carbachol on PI decomposition(P0.05). Conclusions M 3R is much related to IP 3.

[Objective]To evaluate the results of treatment with arthroscope and high tibial osteotomy with fixation of staple made of retention alligation for osteoarthritis of the knee,and to discuss the indication for this technique.[Method]From March 1999 to May 2003,42 arthroscopic treatment and high tibial osteotomies were performed in 34 patients.There were 6 men(8 knees)and 28 women(34 knees,with a mean age of 54.2 years(ranged,42～67 years.The average postoperative follow-up was 36 months(ranged,16～44 months.The indications for high tibial osteteotomy were unicompartmental osteoarthritis and varus malalignment.After arthroscopic treatment,a lateral closing-wedge osteotomy was performed with fixation of staple made of retention alligation.The arthroscopic examination showed:plica synovialis in l0 knees,medial meniscus injuries in 12 knees,lateral meniscus injuries in 6 knees,articular cartilage injuries in 20 knees,body loose in joint in 7 knees,intercondylar fossa stenosis in 9 knees.Common peroneal nerve was not exposed and the periosteum opposite to the insicion position was left intact for sake of stability and acted as a hinge around which the wedge osteotomy was closed.The patients were reexamined to obtain a knee score,to make lateral andanteroposterior radiograph of the involved knee with the patient standing.[Result]During the follow-up,the patients showed satifying pain relief,improvement of joint function,correction of yams deformity.The average preoperative knee score was 53.714?6.7,The average postoperative knee score at the time of the latest follow-up was 91.02?7.7.Comparing preoperative with postoperative,there were significant differences(P

Objective To study the role of the expression of P-selectin and intercellular adhesion molecule-1(ICAM-1) in hamster to rat concordant cardiac xenotransplantation.Methods Intra-(abdominal) cardiac transplantation was performed by using SD rats as recipients of Golden Syrian(hamster) hearts.The rats were divided into 4 groups randomly: group I(n=15),unmodified(recipients) were used as control; group II(n=15),splenectomy at day 0;group III(n=15),the rats were treated with cyclosporine A(CsA) 10 mg/kg every day from day 0;group IV(n=15),splenectomy in combined with CsA.The survival of hamster hearts was observed in each group.The xenograft was harvested at rejection and scheduled time in accordance with the experimental design,and analyzed for histology.The expression of P-selectin and ICAM-1 was detected by immunohistochemistry in xenograft after xenotransplantation.Results The mean survival time of the xenograft in group IV was 34.20?8.98 days,which was significantly longer in groups I,II and III (P