Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

ObjectiveTo study the correlation between blast lung injury (BLI) and acute posttraumatic stress disorder(PTSD) through the establishment of the model about blasting injury of rats.Methods84 SD male rats were randomly divided into four groups:the normal control group( A),the model groups ( B,C) and the sham model group(D).21 rats in each group.The chest of the model group and sham model group rats,respectively,were exposed to 8＃ instantaneous electric detonator at different distances.Then,emotion and behavior of rats was observed through open field test and elevated plus maze test,at day 1,14,28 after blasting.Group A was not given the explosion of stress treatment.After testing,each group was randomly picked up seven rats for succumbing to draw blood which was used to detecting the level of plasma corticosterone by radioimmunoassay,respectively.Results( 1 ) On day 1,it was a significant difference that the latency time ( LT),crossing frequency (CF),rearing frequency(RF) and modification frequency(MF) which were both group B( (25.34 ± 4.50)s,( 19.73 ± 11.10 ),(0.52±0.11),(0.53±0.21) respectively)and group C((22.13 ±6.54)s,(28.67 ±7.22),(1.07 ±0.26),( 0.89 ± 0.15 ) respectively ) were compared with group A,including LT,CF and RF of group D ( ( 23.71 ± 3.9 ) s,(25.13 ± 12.35 ),( 1.26 ± 0.74 ) respectively ) (P ＜ 0.05 ).Then,on day 28,although there was a significant difference which both the LT,CF and RF of group B,and the CF and RF of group C were compared with group A(P ＜ 0.05 ),no significant discrepancy was observed between group B and C (P ＞ 0.05 ).( 2 ) In the elevated plus maze test,the result was similar to the open field test.(3)On day 1 after blasting injury,it was significant distinction that the level of serum corticosterone of group B,C and D (( 153.15 ± 44.50) ng/ml,( 132.72 ±78.77)ng/ml,(96.16 ±8.96)ng/ml respectively)were higher than group A(P＜0.05).Finally,the level of serum corticosterone of group B,C and D were lower than group A on day 28 (P ＜ 0.05 ).ConclusionBLI model successfully induces acute stress disorder(ASD) of rots which behaved like anxiety,fear,hyperarousal and avoidance,and promots furtherly the development of ASD to PTSD,and establishes the modle of acute PTSD.

To explore the effect of comprehensive management on psychological influence in children with asthma, 60 cases with asthma was administrated by WHO global comprehensive strategy for the management of asthma including the persistent use of beclomethaone with the intermittent inhalation of salbutanmol, the lung function monitored by peak flow meter, the detection and avoidance of the induced factors, the management of the social education and the psychological intervention. All children on comprehensive management were followed one year for observation. The results showed that the rate of the total effect was 98.3%. There were all decrease significantly in the rate of the hospitalization, the rate of the emergency, and the rate of the absence for school. It was obvious that asthmatic children and their parents's confidence for the treatment of asthma enhanced significantly.Their psychological disturbance, the school grade, and the life quality were also obtained advanced improvement.

We compared the total errors observed from both precision and accuracy validation experiments with defined medically allowable errors for each quantitative test,and used Westgard's method evaluation decision chart for initial judgement of the acceptability of method validation performance. Moreover,we need to judge the acceptability of other method validation parameters,so that we could integratedly assess and judge whether the testing system is acceptable to use in the laboratory.

Arterial Occlusive Diseases ; diagnosis ; etiology ; Coronary Disease ; complications ; diagnosis ; Humans ; Lower Extremity ; blood supply ; Male ; Middle Aged

BACKGROUND: Rhodiola rosco L acts on anti-aging,anti-fatigue and improving immunity in human body and improves angina pectoris.OBJECTIVE: To study the effect of rhodiola root on heart hemodynamics and myocardial contractility and probe into its mechanism.DESIGN: Observational and controlled based on the experimental animals.SETTING: Department of physiology in a medical university.MATERIALS: The experiment was performed in Laboratory of Cardiac Vessels of Department of Physiology of Guangxi Medical University,from January to April 2001.Thirty New Zealand white rabbits of either sex,weighted varied from 1.9 to 2.3 kg,were randomized into two groups,named as the control(physiological saline 4 mL/kg) and rhodiola sacra SH Fu (nuodikang) group(0.5 g/4 mL · kg-1),15 rabbits in each group. A hundred common wild frogs were employed,weighted varied from 120 to 160 g,randomized into 4 groups, named as the control group of physiological saline,nuodikang of small dosage group(blood concentration 209 mg/L),nuodikang of moderate dosage group(blood concentration 418 mg/L) and nuodikang of large dosage group(blood concentration 627 mg/L),25 frogs in each group.METHODS: ms2000 multiple medium biological signal analysis system was applied,by Straub method,frog heart perfusion specimen in vitro was prepared and the cardiac activity was assayed. The relevant indexes of ventricle activity in rabbits were determined with hemodynamics.MAIN OUTCOME MEASURES: Comparison of hemodynamics changes in rabbits of two groups and the comparison of effects of nuodikang at various dosages on frog heart work in vitro.RESULTS: Myocardial contractility in every nuodikang group was strengthened remarkably compared with the control(F = 8.939, P<0.01 ), the myocardial contractility in nuodikang groups at the dosages of 209 mg/L,418 mg/L and 627 mg/L was in the tendency of increasing,strengthened by 52. 3%,70. 1% and 86.4% respectively. But the significant difference was not apparent in the comparison among experimental groups(P>0.05). The significant difference in frog heart rates among groups was not apparent(P>0.05). Concerning to hemodynamics in rabbits,in nuodikang groups,the maximum increasing rate of pressure in the left ventricle was increased by 22.1% (F = 6.259,P<0.05),the mean pressure of artery was decreased by 8.2% (F = 5.688, P<0.05) . The significant difference in other indexes was not apparent in the comparison between the control and nuodikang groups.CONCLUSION: Nuodikang strengthens myocardial contractility and speeds up myocardial contraction, but does not present remarkable influence on myocardial dilation; simultaneously it reduces the mean pressure of artery.

Objective To understand the actual psychological conditions of pregnant patients with gynecologic malignancies during chemotherapy so as to instruct the nurses to provide individualized psychological care to the patients and their relatives. Method The in-depth interviews were conducted to 7 patients by implementing the phenomenological approach of qualitative study. The acquired data were analyzed. Result The patients experienced four psychological conditions: pessimism about prognosis, anxiety to know more about the chemotherapy and guilty to their family and hope for treatment effect. Conclusion Nurses should pay attention to the psychological conditions of pregnant patients with gynecologic malignancies and help them successfully live through the treatment.

Objective To study the relationship between IP 3 and detrusor cell contraction mediated by M 3R. Methods [3H]-IP contents of human cultured detrusor cells were detected after stimulated by carbachol,atropine,methoctramine and 4-DAMP respectively. Results [3H]-IP contents increased with carbachol concentration.On the different concentrations (10 -9、10 -8、10 -7、10 -6、10 -5、10 -4 mmol/L )of 4-DAMP,[3H]-IP contents were 3 926.57?273.29、2 780.52?211.09、2436.84?153.62、1 973.22?164.71、1 372.38?141.35 and 1 107.98?920.45 cpm respectively.On the some concertrations of Atropine,[3H]-IP contents were 3 602.69?280.17,2 891.31?207.45,1 983.97?145.74,1 269.57? 105.31,1 106.37?75.23,927.50?77.36/min,respectively.On the same concentrations of methoctramine, the [3H]-IP contents was 4 462.74?360.69、3 938.61?327.13、3 315.45?270.36、3 063.19? 246.79、2927.37?226.45 and 2 836.55?241.63 cpm.This donoted that 4-DAMP and atropine signficantly inhibited the effect of carbachol on PI decomposition(P0.05). Conclusions M 3R is much related to IP 3.

Objective To investigate the effect of liver transplantation on extensive intrahepatic duct stones with biliary cirrhosis and unresectable intrahepatic alveolar echinococcosis.Methods Orthotopic liver transplantation was performed on 2 patients with extensive intrahepatic stones with biliary cirrhosis and 4 cases of unresectable alveolar echinococcosis. The pre- and intraoperative condition and postoperative complications were evaluated. All patients were regularly followed up. Results Two patients with intrahepatic stones have survived for more than 2 years after the transplantation. Three of 4 patients with alveolar echinococcosis have survived for 9, 15 months and 2 years respectively, up to now. Another one died of heart failure at the postoperative 3rd month. All survivors have recovered well their normal life and work. Conclusion Liver transplantation could be regarded as effective therapeutic means for extensive intrahepa- tic stones and unresectable alveolar echinococcosis.

[Objective]To evaluate the results of treatment with arthroscope and high tibial osteotomy with fixation of staple made of retention alligation for osteoarthritis of the knee,and to discuss the indication for this technique.[Method]From March 1999 to May 2003,42 arthroscopic treatment and high tibial osteotomies were performed in 34 patients.There were 6 men(8 knees)and 28 women(34 knees,with a mean age of 54.2 years(ranged,42～67 years.The average postoperative follow-up was 36 months(ranged,16～44 months.The indications for high tibial osteteotomy were unicompartmental osteoarthritis and varus malalignment.After arthroscopic treatment,a lateral closing-wedge osteotomy was performed with fixation of staple made of retention alligation.The arthroscopic examination showed:plica synovialis in l0 knees,medial meniscus injuries in 12 knees,lateral meniscus injuries in 6 knees,articular cartilage injuries in 20 knees,body loose in joint in 7 knees,intercondylar fossa stenosis in 9 knees.Common peroneal nerve was not exposed and the periosteum opposite to the insicion position was left intact for sake of stability and acted as a hinge around which the wedge osteotomy was closed.The patients were reexamined to obtain a knee score,to make lateral andanteroposterior radiograph of the involved knee with the patient standing.[Result]During the follow-up,the patients showed satifying pain relief,improvement of joint function,correction of yams deformity.The average preoperative knee score was 53.714?6.7,The average postoperative knee score at the time of the latest follow-up was 91.02?7.7.Comparing preoperative with postoperative,there were significant differences(P