Viniferin is the generic term of oligomers of resveratrol, which acts as phytoalexin in Leguminosae, Polygonaceae, Vitaceae, Ranunculaceae, Dipterocarpaceae and other plants. Viniferin plays important physiological roles in protecting against UV damage and resisting bacterial fungal and viral infection in plants. Nevertheless, these oligomers have shown various pharmacological activities including antioxidative activities, anti-pathogenic, anti-inflammatory and anti-tumor activities. This paper review the recent advances in research of viniferins microbes to show their key pharmaceutical activities for pharmaceutic references.

OBJECTIVE To investigate the pharmacodynamics of chronopharmaceutical drug delivery of erlotinib in lung cancer model nude mice and its potential mechanism. METHODS A nude mouse model of human lung adenocarcinoma HCC827 cell subcutaneously implanted tumor was established and subsequently the nude mice were randomly divided into 6 erlotinib groups and a model group, with 10 nude mouse per group. Erlotinib groups were respectively gavaged with 5 mg.kg-1 erlotinib at 08:00, 12:00, 16:00, 20:00, 24:00 and morrow 04:00, while the model group was given the same volume fraction of captisol. The tumor volume and tumor mass were measured and the tumor growth inhibitory rate was calculated. The mRNA expression of epidermal growth factor receptor (EGFR), mitogen-acti-vated protein kinase (MAPK), cyclin-dependent kinase inhibitor 1A(P21Waf1) and the related protein level were detected by real-time quantitative PCR and Western blotting. RESULTS Compared with model group, the tumor volume and tumor mass of mice at the dosing time of 08:00 and morrow 04:00 were significantly decreased(P<0.05). Compared with dosing time at 20:00 group〔(0.70±0.36)g〕, the tumor mass of 08:00 group〔(0.30±0.17)g〕 and morrow 04:00〔(0.39±0.29)g〕 group was significantly decreased(P<0.05). The mRNA expression of EGFR and MAPK in the tumor group of 08:00 was lower than in group of 20:00(P<0.05), while the mRNA expression of P21Waf1 was significantly higher than that of model group( P <0.05). Compared with model group, the protein expression of p-EGFR and p-MAPK in tumor 08: 00 and morrow 04: 00 group was negative-regulated significantly (P<0.05). CONCLUSION The antitumor effect of erlotinib on the human lung adenocarcinoma implanted tumor nude mice model presents rhythmicity. The dosing time at 08:00 is the most effective. lts mechanism is likely related to EGFR/ MAPK/ P21Waf1 signal transduction pathway.

Brain natriuretic peptide (BNP) is a major marker for evaluating cardiac function and has been widely used in clinical practice. Recent researches show that BNP is also useful for identification of sudden cardiac death in forensic pathology. This article reviews the molecular structure and biological characteristics of the BNP and its application as a functional indicate in forensic medicine. It shows that the expression of BNP in cardiac muscles, together with the expression of BNP in blood and pericardium liquid can be used to evaluate the pathological physiology changes and dysfunction degrees of the heart during the cardiac sudden death.
Amino Acid Sequence ; Animals ; Autopsy ; Biomarkers ; Death, Sudden, Cardiac ; Forensic Pathology ; Heart Diseases/physiopathology* ; Heart Failure/physiopathology* ; Humans ; Immunohistochemistry ; Molecular Sequence Data ; Myocardium/pathology* ; Natriuretic Peptide, Brain/metabolism* ; Peptide Fragments/metabolism* ; Pericardium/metabolism* ; Postmortem Changes ; RNA, Messenger/metabolism*

Aim To investigate whether DCP has pro- teetive effeet on 2 ,4-dimethylnitrosamine ( DMN) -induced liver fibrosis rat model and its effect on MAPK signaling pathway.Methods Hats were intraperitoneal ly injected with DMN to establish HF model,and then were randomly divided into five groups, namely model group, colchicine group, DCP low-dose, medium-dose and high-dose groups,and control group.The rats were given DMN continuously for six weeks.Serum was col-lected afterwards to detect biochemical indexes of liver function.HE and Masson staining and immunohisto- chemical experiments were performed on liver tissues.RT-PCR was applied to detect the expression of inflammatory factors.Western blot was used to detect the ex pression of proteins related to MAPK pathway,the preventive effect of DCP on HF was observed, and its in-tervention effect on MAPK pathway was explored.Results The liver function of rats in model group was severely impaired, with obvious hepatocyte damage, inflammatory cell infiltration and increased interstitial fibrosis , suggesting that the preparation of HF model was successful.Conclusions DCP can interfere with MAPK signaling pathway to inhibit the inflammatory response and alleviate the progression of HF in rats.