MicroRNA(miRNA) is a class of small noncoding RNA molecules that regulate gene expression by functioning as a post-transcriptional regulatory factor basing on pairing with target mRNA,leading to mRNA cleavage or translation repression,and playing an important role in several fundamental biological and pathological processes.Consequently,nearly every biological process is related to miRNA.MicroRNA-21 is a typical miRNA,which has been found in recent years.It has been shown to participate in the development and prognosis of the pathogenesis of acute kidney injury,IgA nephropathy,renal cell carcinoma and other renal disea ses.Therefore,microRNA-21 may be a new target for the treatment of these diseases in the future.This paper reviews the correlative literature and research development regarding the roles of microRNA-21 in kidney disease,and providing references for diagnosis and treatment of kidney disease in the future.

Objective To discuss the clinical effects of enteral immunonutrition on patients with hyperlipidemic acute pancreatitis and its influence on levels of immunologic function and nutrition indicators.Methods 2015,100 patients with hyperlipidemic acute pancreatitis Selected from department of general surgery between January 2012 and December were randomly divided into observation group and control group,and each group had 50 cases.All patients were treated with conventional treatment,monitored for vital signs,fasting water,gastrointestinal decompression,anti-infection,acid suppression therapy and suppression of trypsin activity,balance of water electrolyte and acid-basewere included.After bowel function in patients with recovery,two groups were given enteral nutrition support,observation group were received enteral immunonutrition therapy.At the same time,we added the immune enhancement components.After treatment for 10 days,we compare two groups of patients with therapeutic effect,for APACHE-Ⅱ score,nutrition indicators (ALB,PAB),immune index(TLC,IgA,IgG,IgM) changes.Results After two groups of patients were given different enteral nutrient solution,the clinical total effective rate of observation group was obviously higher than that of control group(P ＜ 0.05).After treatment for 10 days,the indicators were obviously improved.The APACHE-Ⅱ score of the observation group were significantly fallinger than that of the control group (t =3.311,P ＜0.05).The ALB and PAB levels of the observation group were significantly higher than those of the control group (t =2.217,6.622,P ＜ 0.05).At the same time,the TLC,IgA,IgG,IgM of the observation group were improved significantly better than the control group (t =3.600,4.867,3.270,3.911,P ＜ 0.05).Conclusion Enteral immunonutrition can improve the nutrition indicators and immunological function of patients with hyperlipidemic acute pancreatitis,thus improve prognosis and promoting recovery of the patient.

BACKGROUND: Achilles peritendinitis can change characteristics of mechanical and viscoelastic properties in Achilles tendon, especially the biomechanical properties of Achilles tendon after heparin local injection. OBJECTIVE: To examine the changes of mechanical and viscoelastic properties in Achilles tendon of overuse injuries and the effects of heparin on the mechanical and viscoelastic properties in Achilles tendon. DESIGN, TIME AND SETTIMG: Control animal specimen study was performed at the Biomechanical Laboratory of Sichuan Province Orthopaedic Hospital between March and December 2005. MATERIALS: Fifty Achilles tendon samples from Japanese White rabbits weighing (4.10±0.23) kg were used in this study. METHODS: Rabbits were divided into a control group (n=20), an exercise group (n=12) and a heparin treatment group (n= 18) at random. The rabbits were exercised for 3 weeks, simultaneously the heparin solution were injected into the peritendineum around the insertion of Achilles-calcaneus from the 4th week to the 6th week, twice a week. Then the cyclic stretching on the Achilles tendon was performed for 25 cycles and the tendon was stretched to the constant load to determine the stress relaxation vs, time. Finally the preparation was loaded to failure to measure the biomechanical characteristics of the tendon. MAIN OUTCOME MEARES: The cross-sectional area of the tendon, the cyclic creep lengths versus time, hysteresis loop, the stress relaxation versus time, the strength properties and the stress versus strain characteristics. RESULTS: The normalized area of hysteresis from exercise and heparin groups were significantly reduced in the most cycles, and the stress relaxation reduced more quickly in the exercise and heparin groups. The mean values for yield load and failure energy, yield energy, and yield stress, strain energies from the exercised preparations were higher than those of the controls. All of the mechanical properties from the heparin treated preparations were improved significantly. CONCLUSION: The results showed that the tensile strength and viscoelastic properties of tendon were improved by the management of heparin. It is indicated that the local injections of heparin at the location of peritendineum and tendon-bone insertions are beneficial to viscoelastic and tensile behaviors of the Achilles tendon system.

Objective:To investigate the effects of losartan combined with Bailing capsule on oxidative stress, renal function and microinflammation in patients with chronic kidney disease. Methods:Ninety-four patients with chronic kidney disease who received treatment in Rong Jun Hospital from September 2018 to September 2019 were included in this study. They were randomly assigned to receive treatment with either oral losartan (control group, n = 47) or oral losartan combined with Bailing capsule (treatment group, n = 47) for 24 weeks. Preoperative and postoperative oxidative stress, renal function and microinflammatory factor level as well as clinical efficacy were compared between the control and treatment groups. Results:Total effective rate in the treatment group was significantly higher than that in the control group [91.49% (43/47) vs. 68.09% (32/47), χ 2 = 7.982, P < 0.05]. After treatment, superoxide dismutase and glutathione peroxidase levels in the treatment group were significantly higher than those in the control group [superoxide dismutase: (138.16 ± 5.67) U/L vs. (119.74 ± 3.64) U/L, glutathione peroxidase: (48.20 ± 3.78) U/L vs. (41.36 ± 3.29) U/L, t =18.742, 9.358, both P < 0.05]. After treatment, malondialdehyde level in the observation group was significantly lower than that in the control group [(2.78 ± 0.32) mmol/L vs. (3.21 ± 0.38) mmol/L, t = 5.934, P < 0.05]. After treatment, serum urea nitrogen [(4.32 ± 0.45) mmol/L] and creatinine [(54.45 ± 8.98) mmol/L] in the treatment group were significantly lower than those in the control group [(6.53 ± 0.62) mmol/L and (78.31 ± 10.16) mmol/L, t = 19.777, 12.063, both P < 0.05]. After treatment, serum levels of tumor necrosis factor-α [(58.36 ± 6.64) μg/L], hypersensitive C-reactive protein [(3.94 ± 1.25) mg/L] and interleukin-18 [(176.58 ± 17.49) ng/L] in the treatment group were significantly lower than those in the control group [(71.32 ± 6.07) μg/L, (6.87 ± 1.49) mg/L and (229.31 ± 20.09) ng/L, t = 9.876, 10.328, 13.572, all P < 0.05]. Conclusion:Losartan combined with Bailing capsule can improve oxidative stress, renal function and microinflammation in patients with chronic kidney disease and can achieve good clinical efficacy.

Objective To understand the sanitary quality of ground water in countryside of Tongzhou District, Beijing, and to provide basic data for water improvment in countryside in future. Methods The water sampls collected from wells by stratified sampling method in every township of Tongzhou District, Beijing were analyzed. Results The sanitarg quality of source water samples from every township showed significant differences. The completely-qualified rate of source water was 100% in Maqiao, 0% in Xuxinzhuang, Langfu, Gantang. 13 indexes among 26 determined sanitary indexes including ammonia nitrogen, turbidity, total iron, and manganese exceeded the related standards. The concentrations of total hardness, arsenic, fluoride nitrite exceeded the related standards. Regional differences in contaminative degree were noticed. Conclusion The ground water in countryside of Tongzhou District was contaminated in different degree, and show regional differences in water quality, which needed to be improved urgently.

8 m. Class Ⅱ: phonation time 6-9 s, and 6-9 syllables; clear pronunciation, fluent expression, audible distance 5 m. Class Ⅲ: phonation time 80 dB was regarded as normal. ③Language diagram: Normal vowels were characterized by sine harmonic wave, even and regular flow mark, clear first and second resonance peaks and little noise in high frequency area above 4 000 Hz. RESULTS: All 60 patients were included in final analysis. ①After professional instruction and training, 39 cases reached the class Ⅰ, 14 reached class Ⅱ, and only 7 was at class Ⅲ. ②The maximum mean sound intensity was 85.5 dB. ③The language diagram characteristics of the patients was similar to healthy persons. The sound energy predominantly located at low and medium frequency areas, and gradually decreased in high frequency area. CONCLUSION: The pneumatic artificial larynx is characterized by simple operation, clear speech, nearly normal sound intensity, tone, and tone color. It is worthy of extensive application in clinic.

Objective:Toinvestigate the effects ofantisense oligonucleotide(ASODN)targetinghsa-miR-518b on the growth and cycle of gastric cancer cell,and the mechanism in gastric cancer.Methods:Specific phosphorothioate antisense oligodeoxynucleotides targeting hsa-miR-518b were synthesized and then transfected into the gastric cancer cel(lSGC7901).We investigated the change of the hsa-miR-518b expression in SGC7901 after the transfection by using real time RT-PCR.Methyl thiazolyl tetrazolium(MTT)assay was used to detect the cell growth of SGC 7901 and fluorescence activated cell sorting(FACS)was used to analyze the cell cycle of SGC7901.Results:After transfection,the expression of hsa-miR-518b was decrased(P=0.000),the growth of SGC7901 was suppressed(P=0.000) and the cell cycle arrested in G(1P=0.000).Conclusion:hsa-miR-518b(ASODN)could suppress the growth of SGC 7901 by arresting the cell cycle in G1 phase.Hsa-miR-518b could promote the growth of gastric cancer cell by regulating Gl/S transition.

Objectives:To explorer the influence of siRNA-Smad3-mediated Smad3 gene silence on the expression of fibronectin and collagenⅠin human keloid fibroblast(sKFB).Methods:One specific Smad3-siRNA fragment and another irrelevant siRNA fragment was respectively transfected into human primary KFB cells.RT-PCR,Western blot,immunohistochemisty and immunofluorescence were employed to evaluate the changes in either the expression or the secretion of both fibronectin and collagenⅠ.Restults:The expression and secretion of both the fibronectin and collagenⅠin KFB were inhibited significantly by the treatment of Smad3-siRNA for 48 hours at a final concentration of 75nmol/L as compared with those of the untreated or the irrelevant siRNA fragment treated group.Conculsion:Smad3-siRNA could be employed to specifically inhibit the Smad3 gene expression in KFB cells and to efficiently inhibit both the synthesis and secretion of fibronectin and collagenⅠ.

Objective: The galvanic corrosion behavior of dental amalgam coupled to three types of dental alloys in common use(pure Ti?Ni-Cr alloy and Co-Cr alloy),was investigated in artificial saliva.Methods: An electrochemical method was used in the measurement of corrosion potential(Ecorr)?polarization resistance(Rp) and corrosion current density(Icorr) of four types of alloys,and current density of three galvanic couples.These electrochemical parameters were analyzed statistically.Results: The corrosion potential,polarization resistance and corrosion current density,respectively,were statistically different among the different alloys(P

Objective To study the effects of metformin on the blood glucose (BG), insulin (Ins), serum plasminogen activator inhibitor-1 (PAI-1) and blood lipid levels in diabetic patients. Methods Clinical data of 124 cases of diabetic patients admitted between Jan. 2010 and Dec. 2012 were retrospectively analyzed. The diabetic patients were randomly divided into the observation group (62 cases) and control group (62 cases). Patients in the observation group were treated with metformin, while the control group underwent Gliclazide sustained-release tablets treatment. Changes in BG, Ins, PAI-1 and blood lipid levels of the two groups were observed, and the contrast analysis was carried out. Results The BG, Ins and PAI-I levels of the observation group was significantly lower than that of the control group, and the differences were statistically significant (P<0.05).The cholesterol, triglyceride and low density lipoprotein cholesterol levels of patients in the observation group were significantly lower than that of the control group, and the differences were statistically significant (P<0.05). Standard deviation of NN intervals (SDNN), root mean square of successive differences (RMSSD) and percentage of adjacent NN intervals in a 24 hour recording which differ by at least 50 ms (PNN50) of the observation group were significantly higher than that of the control group, and the differences were statistically significant (P<0.05). Conclusions Metformin can effectively control the BG, Ins, PAI-1 and blood lipid levels of diabetic patient and improve the SDNN, RMSSD and PNN50, which is very favorable for the patients. Along with good safety, Metformin is suitable for clinical recommendation.