Objective To facilitate a better understanding of the progress in the research, diagnosis and treatment of Peutz Jeghers syndrome (PJS).Methods Almost all the papers related to PJS from various magazines published in English and Chinese in recent years were reviewed. Current progresses in PJS research and related diagnosis and treatment were discussed in this review.Results PJS is a rare inherited disease with autosomal dominant trait, which is characterized by the presence of hamartomatous gastrointestinal polyps and mucocutaneous pigmentation of the lips, buccal mucosa, and digits. This syndrome is commonly complicated with intestinal obstruction, bleeding, or intussusception,and patients with this disease are at high risk for the development of both GI and extraintestinal malignancies. STK11 on chromosome 19p13.3 are responsible for most cases of PJS. The polyps of PJS tend to have a high incidence of malignant change, and the recurrence of malignancy after treatment is also high. Conclusion The STK11 has been identified as one of the main genes responsible for PJS and has close correlation with formation and development of tumors. Patients with PJS are at high risk for the development of both GI and extraintestinal malignancies.

OBJECTIVETo investigate the therapeutic effects of manipulative reduction for calcaneal fractures.

METHODSFrom January 2009 to June 2012, 53 cases (60 affected feet) of Sanders type I-IV calcaneal fractures were treated by manipulative reduction and external fixation with cardboard splint,including 45 males and 8 females with an average age of (33.5 +/- 1.54) years old ranging from 18 to 65. The course of disease ranged 0.5 h to 7 d. Before treatment the feet were swelling, ache and activity limitation in evidence, some feet were wide flat deformity, the cortical bone was broken in the imaging examination. All selected cases were evaluated using Creighton-Nebraska health foundation assessment scale for fractures of calcaneus.

RESULTSAll cases were followed up at 12 months after treatment, 13 feet got excellent curative effect, 34 good, 11 moderate and 2 poor.

CONCLUSIONManipulative reduction for Sanders type I-IV calcaneal fractures could get excellent curative effect. Manipulative reduction for calcaneal fractures could avoid surgical trauma while assure high curative effects. Manipulative reduction is not only economical and easy therapy, but also can restore maximum function of the calcaneus with few complications and facilitate early rehabilitation of ankle and joint function.

Adolescent ; Adult ; Aged ; Calcaneus ; injuries ; surgery ; External Fixators ; Female ; Fractures, Bone ; rehabilitation ; surgery ; Humans ; Male ; Manipulation, Orthopedic ; methods ; Middle Aged ; Splints

Chromosome Aberrations ; radiation effects ; DNA Damage ; radiation effects ; Electromagnetic Fields ; adverse effects ; Sister Chromatid Exchange ; radiation effects

Objective To investigate the imaging feature of Chinese familial BCS, and to evaluate the efficacy of interventional treatment for Chinese familial BCS. Methods Angiography was taken in 4 familial BCS patients(from families A and B), and PTA was attempted in 2 of 4 patients, stents were placed in another 2 patients. Results Sisters in family A and sisters in family B had membranous obstruction of the inferior vena cava(MOVC) and segmental obstruction of the inferior vena cava(SOVC), respectively. PTA was attempted in younger sisters of the two families, stent was implanted in elder sisters of the two families. Patency of inferior vena cava and clinical symptom improvement were noted in 4 patients after interventional treatment. Restenosis was detected by angiography in sisters of family A after follow up 2 years later, and stenting was performed in the elder sister, PTA was performed in the younger sister once again. The elder sister was dead after the second stenting, and the younger sister had no symptom up to now. Sisters in family B had no restenosis after 4 years′ follow up. Conclusion 1.Lesion types of Chinese familial BCS were varied. 2.Vena cava thrombosis is the etiology of Chinese familial BCS .3.PTA, stenting and long time anticoagulation treatment may be effective methods in the treatment of familial BCS.

Objective To evaluate the safety and effect of percutaneous fenestration of intimal flap(FIF) and endovascular stent(ES) placement for aortic dissection. Methods Male patient, 54 years old. DeBakey Ⅲb aortic dissection, tear of intimal flap situated at the beginning of desconding aorta, developed to abdominal aorta and right iliac artery. The true lumen was 3 mm at narrowest locatation. Through femoral artery approach, percutaneous fenestration of intimal flap and ES placement are operated and four ES were placed. Results The blood flow of aortic true lumen and branches were resumed. The true lumen raised to 12 3 mm at the narrowest locatation. The clinical symptoms vanished. Conclusion Percutaneous fenestration and ES placement for aortic dissection feature little injure, high safety and effecacy. So, It is the first choice for certain aortic dissection.

Objective To evaluate the effi ciency of thrombectomy of iliac-femoral venous thrombosis (I-FVT) via transjugular approach. Methods Seven patients wit h I-FVT were treated with implantation of inferior vena caval filter, balloon- di rected drag, and catheter-directed suction. Some patients were treated with PTA and stent. Meanwhile, strict anti-coagulation was necessary. Results Thrombectom y via transjugular was successful in all 7 patients. The iliac- femoral veins w e re patent after the operation and swollen symptom of lower limb disappeared. The re was swollen of the lower limb in 1 patient after treatment. Symptom disappear ed after PTA or stent treatment again. There were no serious complications. Foll ow-up study showed no pulmonary embolization or recrudescence in all 7 patients . Conclusion Thrombectomy via transjugular approach is a safe an d effective method for the treatment of I-FVT.

Most of the proteomics analysis methods based on tandem mass spectrometry rely on the matching scoring of actual spectrum and theoretical spectrum, the interference of a large number of co-eluting peptides could cause error in the identification and quantification of peptides and proteins. Peptide retention time prediction, as a auxiliary and verification index of the peptide, can transition the chromatographic behavior into stable independent time attributes, and improve the accuracy of the peptide identification. Prediction of peptide chromatographic retention in complex systems is also of great significance for optimizing proteomics determination conditions and improving the detection rate and repeatability of mass spectrometry data in data-independent acquisition. This review focused on the chromatographic retention prediction method of unmodified peptides and modified peptides, summarizes the content, characteristics and limitations of four types of peptide retention time prediction methods based on standardized indexes, peptide molecular models, amino acid residue parameters, and machine learning, as well as their applications in proteomics, with a prospect of their future.

Objective To observe the effect on the patients of diabetes mellitus and cerebral infarction in terms of level of blood rheology and endothelin after using both intravenous low intensity laser irradiation (ILILI) and traditional Chinese medicine Tangnaoluotong in the treatment. Method Ninety patients of diabetes mellitus and cerebral infarction, having the symptom of deficiency in both vital energy and yin with blood stasis, were divided into three treatment groups at random:ILILI group (A), TCM treatment group (B), and a group with compound treatment of ILILI and TCM (C). The level of blood rheology and endothelin were observed after 1 month’s treatment. Result Compared with group A and group B, group C could significantly reduce the level of fibrinogen and endothelin, improve the deformed ability of RBC. Conclusion ILILI with Tangnaoluotong can significantly alter the blood rheology and endothelin on the patients of diabetes mellitus and cerebral infarction, which result in a better curative effect.

Objective To study the inhibitory effects of stachyose on the hyperacute rejection in pig-to-human heart xenotransplantation.Methods A pig-to-human xenogeneic heart transplantation model was established based on an in vitro free heart blood perfusion system.The pig hearts were di- vided into two groups:group A(pig hearts treated with human blood perfusion as control)and group B(pig hearts treated with human blood plus stachyose perfusion).After perfusion for 1h,the heart xenografts were examined for deposit of lgG and IgM by immunohistochemistry and pathological analy sis.Results The mean survival time of perfusion hearts in groups A and B was(9.5?2.5)min and (46.8?8.1)min respectively(P

Objective To compare retrospectively the clinical outcome of total knee replacement with and without patellar replacement. Methods From January 1994 to December 2000, 109 knees in 86 patients treated for osteoarthritis and rheumatoid arthritis were evaluated postoperatively using a questionnaire and physical examination. There were 17 males and 69 females with the age ranging from 37 to 80 years (average 65.7 years). The osteoarthritis was 8 knees and rheumatoid arthritis 69 knees. Forty-two knees underwent patellar replacement, and 67 knees with reserved patella. The patients were scored using HSS Score for knees and Feller Score for patella. AP and lateral views of the knee as well as 30? and 90? axial views of the patella were taken in radiography .The data were analyzed using SPSS software. Results Replacement and non-replacement groups showed no obvious difference in post-operative knee function and the incidence of complication. The two groups showed statistical significant differences in scoring on climbing and descending stairs and rising from sitting position, the results suggested the replacement group scored slightly better than the nonreplacement group. Post-operative anterior knee pain occurred more commonly in the non-replacement group and showed significant difference (P