Objective To investigate the mental health and the general well-being among Hangzhou scientific and technical workers,and the mediating effect of coping strategies in perceived level of stress and mental health status.Methods A stratified cluster sampling method were developed among engineering technicians,agrotechnicians,researchers,health works and teaching staff.510 participants were evaluated with perceived stress of different types,Trait Coping Style Questionnaire (TCSQ),General Well-Being Schedule (GWB),and Self-Reporting Questionnaire 20 (SRQ-20).Results There was significant difference among sub-groups with different educational backgrounds,different occupation,and different age group for total scores of perceived stress (F=5.026,5.637,6.421,P＜0.01).Female had significantly higher scores than male on total scores of negative coping factor of TCSQ,total scores SRQ-20 (26.33±6.29,4.36±3.84 vs 24.93±5.68,3.32±3.53) (all P＜0.05).Total scores of negative coping factor of TCSQ,total scores of positive coping factor of TCSQ,and total scores of perceived stress were the significant predictors of both total scores SRQ-20 and total scores of GWB (all P＜0.001).Both total scores of positive coping factor of TCSQ and total scores of negative coping factor of TCSQ mediated the effects of perceived stress on total scores of SRQ-20 (23.87％,29.33％,respectively) and total scores of GWB (24.29％,23.24％,respectively).Conclusion There do exist the mediating effects between coping strategies in perceived stress and mental health status,the improvement of coping modes of scientific and technical workers can be regarded as a useful intervention strategy to improve their mental health status.

Objective To investigate the feasibility and superiority of enteral-intestinal nutrition after opera- tion of replacement of esophagus with stomach.Methods 62 elderly patients with cancer of esophageal were radom- ly divided into early enteral-intestinal nutrition(EEN) group and total parenteral nutrition(TPN) group,the average cost of hospitalization,recovery situation,postoperative bodyweight,liver function,nutritional status were compared between the two groups.Results The average cost in the early enteral-intestinal nutrition group was less than that in the parenteral nutrition group,the bodyweight in two group were declined and the level of lactate dehydrogenase (LDH) were increased after operation,the level of transferring ferroprotein was lower in TPN group after operation than that in the EEN group(P

Homoacetogens are a group of microorganisms with application potential to produce chemicals and biofuels by the bioconversion of synthesis gas. In this study, we collected waste activated sludge samples to screen homoacetogens by Hungate anaerobic technique, and studied the effect of pH on acetate and alcohol production from H2/CO2 gas. The mixed culture contained Clostridium ljungdahlii, Lysinibacillus fusiformis and Bacillus cereus. Acetate concentration achieved 31.69 mmol/L when the initial pH was 7. The mixed culture containing homoacetogen could converting H2/CO2 to acetate, which provides an efficient microbial resource for the bioconversion of synthesis gas.
Acetates ; chemistry ; Bacteria ; classification ; Biofuels ; Carbon Dioxide ; Hydrogen ; Sewage ; microbiology

Objective To observe the effect of rapid reduction of physical activity on bone mass (BMC), bone mineral density (BMD)and bone metabolism in growing rats. Methods 48 male SD rats were divided into the high-intensity exercise group (T1, n=12), high tolow-intensity exercise group (T2, n=12), low-intensity exercise group (T3, n=12) and control group (Con, n=12). During the first 6 weeks,the rats in T1 and T2 group ran on treadmill with high intensity, while rats in T3 group took low-intensity treadmill exercise. 6 rats were sacrificedfrom each group at the end of 6th week. BMC, BMD of femur were measured with DEXA. Alkaline phosphatase (ALP), osteocalcin(BGP), tartrate-resistant acid phosphatase (TRACP) were detected with ELISA. The exercise intensity of T2 group was reduced rapidly to alow level as in T3 group, but remained in the other 2 groups. At the end of the second 6 weeks, the other rats were investigated again. ResultsCompared with the first measurements, ALP and BGP of the T2 group decreased significantly (P<0.05), and TRACP increased (P<0.05). There was no significant difference in BMC and BMD of femur (P>0.05). Conclusion Rapid reduction of physical activity can suppressbone formation and stimulate bone resorption of long bone among growing rats.

Adenocarcinoma, Papillary ; metabolism ; pathology ; secondary ; surgery ; Adult ; Diagnosis, Differential ; Female ; Follow-Up Studies ; Humans ; Keratin-7 ; metabolism ; Keratins ; metabolism ; Lung Neoplasms ; metabolism ; pathology ; secondary ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; surgery ; Nuclear Proteins ; metabolism ; Thyroid Neoplasms ; metabolism ; pathology ; secondary ; Thyroid Nuclear Factor 1 ; Transcription Factors ; metabolism

Objective To investigate the key points for management of subclinical and agonal types of penetrating cardiac trauma (PCT).Methods A retrospective analysis was conducted on clinical data of 135 PCT cases treated from January 2005 to March 2012.The cases were divided into subclinical type,clinical type (cardiac tamponade or hemorrhagic shock types) and agonal type.Managements of the two extreme types including subclinical type and agonal type were studied in groups.Results (1) Thirty cases of subclinical type failed to have timely diagnosis and treatment due to the withdrawal from inhospital observation,which resulted in 22 deaths.Eleven cases of subclinical type had timely diagnosis through in-hospital observation or cardiac exploration,but three cases died in operating room thoracotomy (ORT).There were 27 cases of agonal type,but 15 died in ORT and two died in emergency room thoracotomy (ERT).Of 67 cases of clinical type,seven died in ORT.(2) A total of 86 cases survived ORT or ERT after timely diagnosis or diagnosis through in-hospital observation plus cardiac exploration.In the meantime,sound recovery was observed in 3-24 months of follow-up.Conclusion Success rate in treatment of PCT can be enhanced by close observation and timely cardiac exploration for subclinical type PCT and by timely ERT and bleeding control for agonal type PCT.

A rapid, sensitive and convenient LC-MS/MS method was developed for the determination of γ-aminobutyric acid (GABA) in human plasma. d2-γ-Aminobutyric acid (d2-GABA) was synthesized as internal standard (IS). After extraction from human plasma by protein precipitation with acetonitrile, all analytes were separated on a Luna HILIC column (100 mm x 3.0 mm, 3 μm) using an isocratic mobile phase of water: acetonitrile: formic acid (20 : 80 : 0.12) with a flow rate of 0.5 mL x min(-1). Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode (MRM) in positive electrospray ionization using the transitions of m/z 104 --> 69 for GABA and m/z 106 --> 71 for d2-GABA. The method was linear in the concentration range of 5.00 to 1 000 ng x mL(-1). The intra- and inter-day precisions were within 9.9%, and accuracy ranged from 99.1% to 104%, within the acceptable limit across all concentrations. The method was successfully applied to a pharmacokinetic study of GABA tablets in healthy Chinese volunteers.
Chromatography, Liquid ; Humans ; Tandem Mass Spectrometry ; gamma-Aminobutyric Acid ; blood

Ice microalgae, which inhabit in the extreme conditions of polar marine environment, not only play an important role in Antarctica ecosystem but also show broad prospects in basic research and application. Ice microalgae have the specific physiological and metabolic properties that are responsible for their adaptation and acclimation to the special polar conditions. Based on the references in the recent twenty years and the partial research results of author's, the ice microalgae composition and distribution, and the application in bait, unsaturated fatty acid, anti-freezing proteins, UV-absorbing pigments and other active substances are reviewed. It is expected to provide references for the research and active substance exploitations of ice microalgae.

Objective To explore the effect of Taichi exercise on bone mineral density (BMD) of postmenopausal women. Methods 105 postmenopausal women were divided into Taichi group (n=54) and control group (n=51). BMD was detected with dual energy X ray absorptiometry (DXA) in both groups. Results The BMD of L1, L2 and shaft of femur were higher in Taichi group than in control group (P<0.05). The BMD of L4, left neck of femur and left wards triangle were significantly higher in the Taichi group than in the control group (P<0.01).The prevalence of postmenopausal osteoporosis (PMOP) was lower in the Taichi group (31.48%) than in the control group (39.22%), but there was no significant difference. The BMD of L1, L2 and shaft of femur, left wards triangle and shaft of femur were higher in postmenopausal women excising Taichi more than 5 years (P<0.05). Conclusion Long-term of Taichi exercise may increase the BMD of the postmenopausal women effectively and prevent the development of PMOP.