Type 2 Diabetes is the consequence of the disturbances of both α- and β-cell secretions.Glucagon-like peptide-1 ( GLP-1 ),which regulates the function of alpha and beta cells,has led to the emergence of a new class of oral anti-diabetic drugs.DPP-4 inhibitors,which increase the concentration of GLP-1 and consequently improve HbAlC,have become a choice for patients in different stages of diabetes.

Objective To investigate the feasibility of segmental splenectomy for treatment of splenic benign tumor. Methods The clinical data of 6 patients with splenic benign tumor treated in our hospital with spleen-preserving surgery in the past 2 years were retrospectively analyzed. Results All the 6 patients successfully underwent surgery and recovered smoothly after operation. No acute severe infection occurred. Ultrasonography showed that the blood supply of residual spleen was of no problem. The components of peripheral blood had no significant changes after operation. Conclusion As long as we know the vascular anatomy of spleen well, segmental splenectomy is safe, reliable and feasible.

Antiviral Agents ; administration & dosage ; therapeutic use ; Hepatitis C ; drug therapy ; Humans ; Liver Cirrhosis ; drug therapy

OBJECTIVE:To establish a method for simultaneous determination of chlorogenic acid,geniposide,gentiopicro-side,baicalin,berberine hydrochloride and chrysophanol in Longze xiongdan capsule. METHODS:HPLC method was adopted. The separation was performed on Dikma Platisil ODS column with mobile phase consisting of acetonitrile-0.1% phosphoric acid so-lution(gradient elution)at the flow rate of 1.0 mL/min. The detection wavelength were set at 254 nm for geniposide,gentiopicro-side,baicalin,berberine hydrochloride and chrysophanol and 327 nm for chlorogenic acid. The column temperature was 30 ℃. RE-SUITS:Linear ranges of chlorogenic acid,geniposide,gentiopicroside,baicalin,berberine hydrochloride and chrysophanol were 0.0082-0.164 μg (r=0.9998),0.027-0.540 μg(r=0.9997),0.02525-0.505 μg(r=0.9998),0.046-0.920 μg(r=0.9997), 0.059-1.180 μg(r=0.9995),0.00805-0.161 μg(r=0.9994),respectively;the limits of quantitation were 0.61,0.59,0.87,0.51, 0.92,0.65 ng,and the limits of detection were 1.67,1.73,1.96,1.82,2.31,1.87 ng. RSDs of precision,stability and reproduc-ibility tests were lower than 2.0%;recoveries were 95.34%-98.27%(RSD=1.2%,n=9),98.18%-101.92%(RSD=1.4%,n=9), 97.36% -101.09%(RSD=1.0% ,n=9),95.24% -98.93%(RSD=1.3% ,n=9), 95.76% -99.34%(RSD=1.5% ,n=9), 95.00%-98.75%(RSD=1.4%,n=9),respectively. CONCLUSIONS:The method is simple,accurate,reproducible and suitable for simultaneous determination of chlorogenic acid,geniposide,gentiopicroside,baicalin,berberine hydrochloride,chrysophanol in Longze xiongdan capsule.

Objective To observe the effect of liraglutide on arteriosclerotic lesions in diabetic ApoE- / - mice via regulating receptor for advanced glycation end-products ( RAGE) expression of aorta. Methods Thirty male ApoE- / - mice (10 weeks) were randomly divided into 3 groups: control group, diabetes group, and diabetes +liraglutide group. All the mice were fed a high-fat diet. Diabetes was induced by 2 intraperitoneal injection of streptozotocin (STZ, 55 mg·kg-1 ·d-1 ). Mice were treated with subcutaneous injection of liraglutide (0. 4 mg· kg-1 ·d-1 ) in diabetes+liraglutide group. After 9 weeks, blood was drawn and the aorta were rapidly procured. The serum levels of advanced glycation end-products ( AGEs), soluble receptor for advanced glycation end-products (sRAGE ), stromal cell-derived factor-1α ( SDF-1α ), as well as total cholesterol and triacylglycerol were measured. Atherosclerotic plaque area was determined by Sudan Ⅳ staining. The expression of RAGE was determined using RT-PCR and western blot, respectively. Results Compared with control group, the serum AGEs, the RAGE protein and mRNA expression, the atherosclerotic lesions were significantly increased in the mice of diabetes group. Compared with diabetes group, the serum AGEs, the RAGE protein and mRNA expression, the atherosclerotic lesions were significantly decreased in the mice of diabetes+liraglutide group. Conclusion Liraglutide is confirmed as to have an anti-atherosclerotic effects and inhibits RAGE expression in diabetic ApoE- / - mice.

Objective To examine the relationships among measures of gait recovery and community integra-tion index in people with brain injury.Methods 142 brain injured patients were chosen in this study.Community Balance and Mobility Scale,Dynamic Gait Index,Ten-Meter Walk Test for gait speed,and the Community Integration Questionnaire (CIQ) was measured.Results Average walk speed was 0.31-5.30 (1.36 ±0.88)m/s;DGI score was 5-24 (18.6 ±5.44) points;CMBS score was 0 -96 (54.1 ±25.3) points;CIQ subscale 6 -29 (16.2 ± 5.1)points,CIQ family subscale was 0-10 (4.8 ±3.0)points;CIQ society subscale was 4-12(8.2 ±2.1)points;CIQ productivity subscale was 0-7(3.8 ±2.2).The correlation between age and Ten-Meter Walk Test was nega-tive significantly (r=-0.50,P<0.05).All 3 balance/gait measures correlated significantly with the CIQ Productiv-ity subscale (r=0.35-0.58,P<0.05).Conclusion The ability of people with brain injury to engage in work/school/volunteer activity may be reduced by impairments in balance and mobility.

Diabetes is common in men and women aged 50 years and older often complicated with peripheral arterial disease(PAD) arousing more serious attention by vascular surgeon and interventional radiologist. The present review of this article put the main focus on curative effect and limitation of intravascular treatment for patients with diabetic PAD, including proper medical imagings for suitable follow-up and formation of regular managing program for accessing limb function and whole body condition. Furthermore the dosage and proceduce of medical thrombolysis in dealing diabetic PVD, together with the possibility of mechanical thrombolysis approach should expecially emphasized. For further objective acknowledgement of interventional therapy for diabetic PVD; the selection of interventional procedune, the management of single or mutliple lesions and post procedural treatment ought to be considered thoroughly before hard.

Arterial systolic pressure variab il ity (ASPV), heart beat interval variability (HRV) and baro-reflex sensitivity ( BRS) were measured by finger arteriole blood pressue monitor system and cardiova scular spectral analysis system. The diabetic patients with cardiovascular auton omic neuropathy show significant changes in ASPV and HRV and decreased BRS. BRS changes earlier than other parameters.

Objective To investigate the expression changes of connexin 43 (Cx43) gene and the functional changes of the gap junction intercellular communication (GJIC) among cultured unstable detrusor cells and their roles in the development of detrusor instability (DI). Methods Forty healthy female Wistar rats were divided into two groups: DI group and normal control group. RT-PCR and Western blot techniques were employed to detect the expression of Cx 43 mRNA and protein in the cultured detrusor cells. Scrape loading dye transfer (SLDT) technique was used to monitor the GJIC among cultured detrusor cells. Results The expression of Cx43 mRNA and protein in DI group was much higher than that in normal control group (P

Objective To explore the effects of the continuous quality improvement ( CQI) on recovery and health of patients with chronic kidney disease ( CKD) .Methods The clinical data of 485 cases with CKD undergoing standard treatment from Jan .30th, 2012 to Dec.30th, 2012 in Shaoxing People's Hospital were retro-spectively analyzed .A professional team introduced four-step intervention of continuous quality change .The nu-tritional status, renal function changes , and patients'cognition and compliance with low protein diet before and af-ter treatment were compared .Results The nutritional status of patients significantly improved after the treat-ment.The patients with score ≥23 points increased 58.14%.After CQI intervention , urinary albumin/creati-nine (mg/g) was calculated and it was 82.17 before treatment and 64.23 after treatment.The difference had sta-tistical significance (t=11.321,P＜0.05).After the treatment, 82.06% patients understood low-protein diet concept .The treatment was implemented well and the overall data improved significantly .Conclusions CQI has beneficial effects on immunity and renal function recovery of patients with CKD .It can significantly improve the patients'nutritional status , and their cognition of low protein diet .