1.Gene Expression of Endothelin-1 and Endothelin Receptor A on Monocrotaline-Induced Pulmonary Hypertension in Rats After Bosentan Treatment.
Kyoung Ah LIM ; Kwan Chang KIM ; Min Sun CHO ; Bo En LEE ; Hae Soon KIM ; Young Mi HONG
Korean Circulation Journal 2010;40(9):459-464
BACKGROUND AND OBJECTIVES: Endothelin (ET)-1, a potent endothelium-derived vasoconstrictor peptide, has a potential pathophysiologic role in pulmonary hypertension. Bosentan, a dual ET receptor (ET(A)/ET(B)) antagonist, is efficacious in treatment of pulmonary hypertension. The objectives of this study were to investigate the expression of ET-1 and ET receptor A (ERA) genes and to evaluate the effect of bosentan in monocrotaline (MCT)-induced pulmonary hypertension. MATERIALS AND METHODS: Four-week-old male Sprague-Dawley rats were treated as follows: control (n=36), subcutaneous (sc) injection of saline; MCT (n=36), sc injection of MCT (60 mg/kg); and bosentan (n=36), sc injection of MCT (60 mg/kg) plus 25 mg/kg/day bosentan orally. RESULTS: Serum ET-1 concentrations in the MCT group were higher than the control group on day 28 and 42. Quantitative analysis of peripheral pulmonary arteries revealed that the increase in medial wall thickness after MCT injection was significantly attenuated in the bosentan group on day 28 and 42. In addition, the increase in the number of intra-acinar muscular arteries after MCT injection was reduced by bosentan on day 14, 28 and 42. The levels of ET-1 and ERA gene expression were significantly increased in the MCT group compared with control group on day 5, and bosentan decreased the expression of ET-1 on day 5. CONCLUSION: ET-1 contributes to the progression of cardiopulmonary pathology in rats with MCT-induced pulmonary hypertension. Administration of bosentan reduced ET-1 gene expression in MCT-induced pulmonary hypertension in rats.
Animals
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Arteries
;
Endothelin-1
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Endothelins
;
Gene Expression
;
Humans
;
Hypertension, Pulmonary
;
Male
;
Monocrotaline
;
Pulmonary Artery
;
Rats
;
Rats, Sprague-Dawley
;
Receptors, Endothelin
;
Sulfonamides
2.Clinical Mnaifestations, Management and Obstetrical Outcome of the Pregnant Women with Aplastin Anemia.
Jong Kwan JUN ; Joong Shin PARK ; Bo Hyun YOON ; Hee Chul SHIN ; En Kyung KIM ; Won Suk SEO ; Bae Hoon LEE ; Joo Chul KIM ; Young Ah KIM
Korean Journal of Perinatology 1998;9(3):252-262
BACKGROUND: Aplastic anemia is an uncommon disease characterized by marrow hypocellularity, resulting in a reduction of the counts of circulating red blood cells, neutrophils, and platelets. The etiology of aplastic anemia remains unknown in the majority of cases. An association of aplastic anemia with pregnancy has been reported but the strength of such a relationship remains somewhat controversial. OBJECTIVE: To evaluate the association of aplastic anemia with pregnancy and the optimal management of patients with aplastic anemia during pregnancy. METHODS: This retrospective study was done by review of medical records of 18 patients with aplastic anemia, of whom 8 patients were diagnosed before pregnancy and 10 patients were diagnosed during pregnancy, who had a total of 25 pregnancies after the diagnosis from January, 1990 to October, 1997 at the Seoul National University Hospital, RESULTS: The incidence of aplastic anemia during pregnancy was one in 806 persons(18/14, 507) in this study. During pregnancy, the circulating blood cell levels decreased in all pregnancies. In all 7 cases that showed a relapse during pregnancy the remission occurred following the termination of pregnancy. 12 patients presenting with severe aplastic anemia during pregnancy had poorer hematological improvement than patients with mild aplastic anemia after termination of pregnancy. CONCLUSION: In all patients with aplastic anemia during pregnancy the circulating blood cell levels decreased. The successful outcome in these cases illustrates the benefit of modern supportive care in the management of such patients. The present observations suggest that a pregnancy which occurs during the long-term remission of aplastic anemia may be allowed to progress to birth.
Anemia*
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Anemia, Aplastic
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Blood Cells
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Bone Marrow
;
Diagnosis
;
Erythrocytes
;
Female
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Humans
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Incidence
;
Medical Records
;
Neutrophils
;
Parturition
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Pregnancy
;
Pregnant Women*
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Recurrence
;
Retrospective Studies
;
Seoul
3.The relationship between umbilical artery doppler velocimetry and pathologic findings of placental vascular bed.
Hyun Jin SONG ; Pyl Ryang LEE ; Gil Sang EUN ; En Kyung KIM ; Bo Hyun YOON ; Hee Chul SYN ; Je Geun CHI ; Syng Wook KIM
Korean Journal of Perinatology 1992;3(2):28-34
No abstract available.
Rheology*
;
Umbilical Arteries*
4.Studies on development of serum-free conditioned media using Vero cells and DMEM with controlled concentration of glucose and pyruvate.
Ju Hwan KIM ; Young Seok SEO ; Hai Bum SONG ; Jung Bo YANG ; Kyung En LEE ; Ki Hwan LEE
Korean Journal of Obstetrics and Gynecology 2010;53(2):143-151
OBJECTIVE: The purpose of this study was to examine in vitro development of early preimplantation mouse embryos in various kind of serum-free conditioned media (SF-VCM) manufactured from DMEM cultured with Vero Cells. METHODS: A total of 846 two-cell mouse embryos were cultured in different kind of SF-VCM. SF-VCMs were divided into SF-VCM-10, -30 and -50 by media volume using DMEM #1 media, and divided into SF-VCM #1, #2 and #3 by controlled concentration of glucose and pyruvate (manufactured by DMEM #1: mixed three volume of DMEM-G (DMEM with glutamine without glucose and pyruvate) and one volume of DMEM-GGP (DMEM with glutamine, glucose, pyruvate), #2: mixed same volume of DMEM-G and DMEM-GGP and #3: mixed one volume of DMEM-G and three volume of DMEM-GGP, respectively). Experimental groups were mainly added 10% SSS, and 20% hFF was added to only Control group co-cultured with Vero cells. Development of embryos was observed every 24 hours. Results between different groups were analyzed using Chi-square test, and considered statistically significant when P-value was less than 0.05. RESULTS: In vitro developmental rate by each cleavage stages of mouse embryos cultured in SF-VCMs with a various volumes were significantly (P<0.05) higher in SF-VCM-30 (morula< or =: 97.2%, Blastocyst (BL)< or =: 97.2%, Hatching BL< or =: 82.2%) than other groups. In the rate of development on in vitro co-culture vs. a various SF-VCMs manufactured by DMEM controlled concentration of glucose and pyruvate, Group I (SF-VCM #1) was higher than other groups in each cleavage stages (morula< or =: 98.1%, Blastocyst (BL)< or =: 97.1%, hatching BL< or =: 81.7%, respectively). Moreover, specially, in the developmental rate into the hatching blastocyst < or = after 96 hours in vitro culture, Group I (81.7%) was significantly higher than control group (67.6%, P<0.05). CONCLUSION: SF-VCM #1 manufactured by volume of 30 mL DMEM #1 media cultured in vitro for 48 hours in 250 mL flask was the most effective on in vitro developmental rate of mouse preimplantation embryos. Therefore, it is expected that SF-VCM #1 has application to human IVF-ET.
Animals
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Blastocyst
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Coculture Techniques
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Culture Media, Conditioned
;
Embryonic Structures
;
Fertilization in Vitro
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Glucose
;
Glutamine
;
Humans
;
Mice
;
Pyruvic Acid
;
Vero Cells
5.A Hospital-based Tabletop Exercise for Pandemic Influenza Preparedness: Design and Evaluation.
Hee Yun SEOL ; Ji Ryang KIM ; Jung Ha MOK ; Bo Ran KWON ; Sun Hee LEE ; Im Soo KWAK ; Jin Woo JUNG ; Jeong su KIM ; Ock Bae KO ; En Hi CHO ; Seong Sun KIM ; Sang Sook SHIN ; Sang Won LEE
Infection and Chemotherapy 2008;40(2):83-92
BACKGROUND: To evaluate the usefulness and compliance of a hospital-based tabletop exercise in setting of pandemic influenza in hospitals. MATERIALS AND METHODS: Tabletop exercise was held in Pusan National University Hospital and forty two hospital employees were invited to participate in the exercise. The scenario for hospital-based tabletop exercise was designed. It consisted of three modules, which simulated the influx and outbreak of H5N1 influenza that was epidemic in Southeast Asia. Pre-, post-exercise surveys were completed by anonymous questions. RESULTS: Thirty-seven (88%) of 42 invited participants attended exercise. All members of the administration group and the ancillary services group participated. But, only 77% members of the clinical services group participated. In pre-exercise survey, priorities of eight goals regarding skills and knowledge during exercise were inquired., The highest priorities pointed out by the respondents were "Increase the knowledge of pandemic influenza" (22%), "Development of strategies for optimal communication among employees within specific department" (19%) and "Development of strategies for improved coordination between facilities within the health system" (19%). Twenty-one (57%) of participants completed the post-exercise surveys. At post-exercise surveys, 81% of the participants stated that the tabletop exercise was extremely or very useful, 86% of the participants also stated that it increased their knowledge of pandemic influenza. CONCLUSION: Tabletop exercise is an effective modality for increasing pandemic influenza preparedness in hospitals, and this method is useful for guiding preparedness activities within the hospital environment. Further studies to determine the appropriate method of discussion, questionnaire, duration of exercise and injection are needed.
Anonyms and Pseudonyms
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Asia, Southeastern
;
Compliance
;
Influenza, Human
;
Pandemics
;
Surveys and Questionnaires
6.A Hospital-based Tabletop Exercise for Pandemic Influenza Preparedness: Design and Evaluation.
Hee Yun SEOL ; Ji Ryang KIM ; Jung Ha MOK ; Bo Ran KWON ; Sun Hee LEE ; Im Soo KWAK ; Jin Woo JUNG ; Jeong su KIM ; Ock Bae KO ; En Hi CHO ; Seong Sun KIM ; Sang Sook SHIN ; Sang Won LEE
Infection and Chemotherapy 2008;40(2):83-92
BACKGROUND: To evaluate the usefulness and compliance of a hospital-based tabletop exercise in setting of pandemic influenza in hospitals. MATERIALS AND METHODS: Tabletop exercise was held in Pusan National University Hospital and forty two hospital employees were invited to participate in the exercise. The scenario for hospital-based tabletop exercise was designed. It consisted of three modules, which simulated the influx and outbreak of H5N1 influenza that was epidemic in Southeast Asia. Pre-, post-exercise surveys were completed by anonymous questions. RESULTS: Thirty-seven (88%) of 42 invited participants attended exercise. All members of the administration group and the ancillary services group participated. But, only 77% members of the clinical services group participated. In pre-exercise survey, priorities of eight goals regarding skills and knowledge during exercise were inquired., The highest priorities pointed out by the respondents were "Increase the knowledge of pandemic influenza" (22%), "Development of strategies for optimal communication among employees within specific department" (19%) and "Development of strategies for improved coordination between facilities within the health system" (19%). Twenty-one (57%) of participants completed the post-exercise surveys. At post-exercise surveys, 81% of the participants stated that the tabletop exercise was extremely or very useful, 86% of the participants also stated that it increased their knowledge of pandemic influenza. CONCLUSION: Tabletop exercise is an effective modality for increasing pandemic influenza preparedness in hospitals, and this method is useful for guiding preparedness activities within the hospital environment. Further studies to determine the appropriate method of discussion, questionnaire, duration of exercise and injection are needed.
Anonyms and Pseudonyms
;
Asia, Southeastern
;
Compliance
;
Influenza, Human
;
Pandemics
;
Surveys and Questionnaires