Objective To explore the correlation of serum high -sensitivity C -reactive protein and ische-mic stroke in elderly people.Methods 120 patients with acute cerebral infarction were selected as the cerebral infarction group,100 healthy people taken examination at the same period were selected as the control group.The lev-els of serum high -sensitivity C -reactive protein in the two groups were detected,and the serum high -sensitivity C -reactive protein levels in patients with different cerebral areas and different neurological function scores were analyzed.Results Serum high -sensitivity C -reactive protein level in the cerebral infarction group was (10.17 ± 3.13)mg/L,which was significantly higher than that of the control group[(0.81 ±0.24)mg/L],the difference was statistically significant(t =29.82,P <0.05).And the serum high -sensitivity C -reactive protein level was gradually increased with cerebral infarct volume increased and the deepening of neurological deficits(P =0.01).Conclusion The serum high -sensitivity C -reactive protein level in elderly patients with ischemic stroke was higher than healthy people,the larger the infarct volume and the more severe neurological deficits,the higher sensitivity C -reactive protein levels,it is worthy of reference.

OBJECTIVE:To observe the efficacy and safety of Xingnaojing injection in the treatment of ischemic stroke. METH-ODS:110 patients with ischemic stroke were randomly divided into control group and observation group. 2 groups were given regu-lation of blood pressure and blood glucose,lipid lowering,aspirin,citicoline and edaravone,mannitol depending on severity and other conventional treatment. Based on it,control group was intravenously infused 250 ml 0.9% Sodium chloride injection,once a day;observation group was additionally intravenously infused 20 ml Xingnaojing injection,adding into 250 ml 0.9% Sodium chlo-ride injection,once a day. The efficacy was judged after 14 d. US National Institutes of Health Stroke Scale(NIHSS),Glasgow coma score(GCS),nitric oxide(NO),nitric oxide synthase(NOS),inducible nitric oxide(iNOS)content in 2 groups were ob-served,and in the incidence of adverse reactions was recorded. RESULTS:The total effective rat in observation group was signifi-cantly higher than control group,the difference was statistically significant(P<0.05). Before treatment,there were no significant differences in the NIHSS,NO,NOS and iNOS contents between 2 groups(P>0.05);after treatment,GCS in 2 groups were sig-nificantly higher than before,and observation group was higher than control group,NIHSS,NO,NOS and iNOS contents were significantly lower than before,and observation group was lower than control group,the differences were statistically significant (P<0.05). And there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLU-SIONS:Based on conventional treatment,Xingnaojing injection has significant efficacy in the treatment of ischemic stroke,with good safety,which may be related that it can reduce NO and GCS.

Objective: To observe preventive and therapeutic effects of Ganlan Jiejiu Yin on acute alcoholic hepatic injury in the rat and the mouse, and to study preliminarily effects on alinine transaminase(ALT) and aspartic transaminase (AST) in serum and hepatic homogenate. Methods: The acute alcoholic hepatic injury model was made by stomach perfusion of white spirit for 5 days, and drunk time and the recovering time were observed every day, and serum ALT on the 3rd day and ALT and AST in serum and hepatic homogenate on the 5th day were determined, and the results were compared with those of Hugan Capsules. Results: Ganlan Jiejiu Yin could significantly decrease the drunk rate, increase the recovery/drunk ratio, and decrease ALT and AST in serum and ALT in hepatic homogenate with significant differences as compared with those of the control group. Conclusion: Ganlan Jiejiu Yin has good drunk-preventing, alcoholism-relieving and liver-protecting actions.

ObjectiveTo observe the effcacy of isoflurane supplemented with ropivacaine for sacral block in indirect inguinal hernia.Methods120 cases of children who were going to be operated in inguinal hernia repairs were randomly divided into isoflurane group( A),ropivacaine group( B ),and isoflurane supplemented with ropivacaine group(C).Sacral block was performed after induction with different anesthetics.Haemodynamics( MAP、HR 、SpO2 ) in three groups were compared,and anesthesia effect,waking time,drug dosage and adverse reactions were analyzed.ResultsHaemodynamics in isoflurane supplemented with ropivacaine group were more stable than the other two groups,and anesthesia effect was better than isoflurane group and ropivacaine group( all P ＜0.05).Moreover,waking time,drug dosage and adverse reactions in group C were statistical differently conpared with controls( all P ＜ 0.05 ).ConclusionIsoflurane supplemented with ropivacaine for sacral block was helpful for respiration and circulation management applied in indirect inguinal hernia and could be a perfect anesthetic methods.

Objective To study the clinical application of 3-dimensional arterial spin labeling technology (3D-ASL) in brain tumors.Methods Twenty patients with the pathological diagnosis of brain tumors were selected in this study,with low grade glioma in 4 cases,the high grade glioma in 2 cases,lymphoma in 1 case,metastatic tumor in 7 cases,meningioma in 6 cases.All patients were appLIed preoperative 3D-ASL and dynamic contrast-enhanced perfusion magnetic sensitive (DSC) MR imaging line two methods,to analyze the value of 3D-ASL in brain tumors.Results 3D-ASL examination lesions in patients with brain tumors might be displayed in high or low perfusion; 3D-ASL and DSC were obtained from tumor tissue blood supply,the local relative cerebral blood flow (rCBF),there was no significant difference (P ＞ 0.05).The high grade glioma in 3D-ASL image showed a high perfusion,rCBF was 3.0 ± 1.3; Low grade gliomas showed low perfusion,rCBF was 0.6 ± 0.2,the rCBF between the high-and low-grade gliomas was significantly different (P ＜ 0.01).Metastatic tumor 3D-ASL showed ring high perfusion,central necrosis and edema area of low perfusion.Lymphoma 3D-ASL showed slightly higher perfusion.Meningioma showed high perfusion,and highest rCBF,3.5 ± 1.9.Conclusions 3D-ASL in the diagnosis of brain tumors,has high repeatability,non-invasive,without contrast agent.3D-ASL can be further developed into the brain blood flow dynamics of conventional evaluation methods,and can be used for brain tumors level assessment,has broad application prospects.

Herpes simplex keratitis (HSK) is the first inflammatory cause for uniocular blindness.The clinical inspection methods commonly used are limited to general observation,while in vivo confocal microscopy (IVCM) can provide details of cellular changes in an intravital HSK.IVCM not only gives more clinical evidence for pathology studying of HSK,but also serves as a new technology for the whole progress of HSK epidemiology.This article reviewed current researches of applying IVCM for HSK clinical observation and summarized these latest results.Morphological changes of each layer of the cornea,the specificity of these changes and clinical application of IVCM had been discussed in this article.

Retinal anatomic structure and physiology function are very complex.Electroretinogram (ERG) is currently the only objective assessment for retinal function.There are groups of devastating diseases characterized by affecting the inner retinal functions.However,compared with its advantages in evaluating the outer retina function,ERG is less sensitive in judging the inner retinal function.The decrease of sensitivity is mainly associated with the crosstalk between the rod and cone systems in the longer visual signal pathways.In the past twenty years,new recording techniques in ERG analysis including oscillatory potentials,ON-OFF responses,photopic negative responses and scotopic threshold response have been developed.These ERG components are generated from the bipolar cells,amacrine cells or retinal ganglion cells and are becoming novel tools to assess the function of the inner retina.Ophthalmologists should fully understand the clinical significance of these ERG components in assessing inner retinal function to better guide the diagnosis and treatment of retinal diseases.

Glaucoma is a degenerative disease of the retina and the optic nerve.The apoptosis of retinal ganglion cells is the pathological basis of glaucoma,but its mechanism has not been fully elucidated.Currently,a large number of studies in vivo and in vitro have demonstrated that retinal glial cells (including Müller cells,astrocytes and microglial cells) are closely related to the development of glaucoma.Retinal glial cells not only release active factors but also express various neurotransmitter receptors,ion channels,surface markers and effector molecules.Therefore,they are actively involved in neuronal information transmission in addition to the traditional view of the role of nutritional support for neurons.In glaucoma,retinal glial cells are activated to undergo a great number of changes in physiology,biochemistry and morphological features.These activated cells initiate different signaling cascades that may serve as a protective role.Meanwhile,they may release toxic factors to start or aggravate the damaging effects on retinal neurons.Usually,both effects occur in glaucoma in parallel,but the underlying mechanisms and signaling pathways are still not clear.More research is needed to understand that how the retinal glial cells are able to rebuild the neuronal function after injury or to promote the neurodamage.With all these unresolved issues in mind,the progress in the study of the mechanism of Müller glial cells,microglial cells and astrocytes participating in the glaucomatous disease were reviewed.

180% elevation of PKC activity in the retinal vasculature.????????except ??? distributed in the membrane and cytosolic fraction in retinal vessels in normal or diabetic rats,the expression of ?????-PKC significantly increase in the membrane pool OD Value 282%?32%?340%?26%?187%?14% respectively ,but ?????-PKC expression significantly decrease in cytosolic pool in retinal vessels of diabetic rats compared with nondiabetic rats,OD value 71%?11%?63%?8%?84%?6%,respectively,however ? isoenzym expression in cytosolic pool significantly increase 208%?6%,compared with normal rats.Conclusion:Diabetes can induce the translocation of ?????-PKC from cytosol to membrane pool,but ? isoform translocated from membrane to cytosol pool in retinal vessels of diabetic rats.