BACKGROUND: By varying the collected blood volume and storage temperature of the blood culture bottles prior to entry in an automated blood culture system, growth of organisms will be affected. METHODS: Blood culture bottles with a 20 mL blood volume per set were stored at 37degrees C (1st period) and room temperature (RT, 2nd period) upon arrival at the laboratory after working hours compared to baseline period (10 mL, RT). The time to detection (TTD) for all strains and the number of days until the final report after bottle entry were compared among the three periods. RESULTS: The median TTD for all strains was 13.5 h, 10.6 h, and 11.3 h in the baseline (N=268), 1st (N=454), and 2nd period (N=370), respectively (P<0.001). The final identification report was available within two days of bottle entry for 12.3%, 30.6% and 15.1% of bottles in the three different periods, respectively (P<0.001). CONCLUSION: Collecting an adequate blood volume is critical to reduce TTD. The preincubation of blood culture bottles at 37degrees C during the night shift might enable earlier final reports than storage at RT for samples with the same collected blood volume.
Blood Volume*

No abstract available.
Blood Volume

At the first step of studying about “the volume of cord blood” in Vietnamse, a total of 50 samples have been collected. We obtained some results: - The mean volume of cord blood was: 50.06 +/- 20,73ml. -The mean number of total mononuclear cells was 352.4 +/- 161.6 (x106). -Some cord blood colections had many total MNC as some larger cord blood harvests. So, it is our belief that total MNC numbers are more useful indicator of clinical utility than more sample volume.
Fetal Blood ; Blood Volume

OBJECTIVEThe same person's pulmonary venous blood volume, left atrial volume and stroke volume were measured by lung CT scans and cardiac CT angiography (CTA). Then their relationships were analyzed in order to investigate the mechanism of breathing control.

METHODSAs we described before, full pulmonary vascular (-0.6mm) volume was accurately calculated by three-dimensional imaging technology from lung CT scan; left atrial volume and stroke volume of left ventricle were calculated from the CTA data. Then the relationships among them were analyzed for estimation of the lung-artery time.

RESULTSThe total volume of lung and pulmonary vascular blood was 3486 ± 783 (2156-4418) ml, and the pulmonary vascular blood volume was 141 ± 20 (105-163) ml. The estimated pulmonary venous volume was 71 ± 10 (52-81) ml. Left atrial volume at the end diastolic was 97 ± 39 (53-165) ml, Stroke volume of left ventricle was 86 ± 16 (60-106) ml. Pulmonary venous volume and the left atrial volume were double of stroke volume(1.7-2.4).

CONCLUSIONThe estimated lung-artery time was three heart beat.

BACKGREOUND: Several formulas for estimating allowable pre-transfusion blood loss were used to reduce unnecessary intraoperative blood replacement. The postoperative predicted hematocrit computed by formula was compared with the actual hematocrit and was tested which formula was more accurate in spine surgery. METHOD: Total blood volume was estimated in spine surgery of 34 patients. The target hematocrit (Hct) was suggested on 30% and the allowable blood loss was computed using the formula 1 and 2. For each patients, simultaneous measurement of blood loss and Hct was obtained at the end of operation. The postoperative predicted Hct by the formula 1 and 2 was calculated and compared with the actual Hct, and the difference between the formula 1 and 2 was evaluated. Allowable blood loss=Estimated blood volume (Initial Hct Target Hct)/Initial Hct ... Formula (1) Allowable blood loss=Estimated blood volume (Initial Hct Target Hct)/Average Hct ... Formula (2) RESULTS: 1) The preoperative Hct was 40.7 3.9%. The postoperative predicted Hct by the formula 1 and 2 were 34.3 4.6 and 34.9 4.3% respectively. The postoperative actual Hct was 30.1 4.6%.2) The difference between the predicted Hct by the formula 1 and the actual Hct was 4.2% (P<0.05). The difference between the predicted Hct by the formula 2 and the actual Hct was 4.8% (P<0.05). The difference between the predicted Hct by the formula 1 and 2 was 0.6% (P<0.05). It was thought that the predicted Hct by the formula 1 was more closer to the actual Hct. CONCLUSIONS: The predicted Hct by both formulas is underestimated when the results compare with the actual Hct. But the predicted Hct by the formula 1 provides a closer results to the actual Hct than the predicted Hct by the formula 2.
Blood Volume ; Hematocrit* ; Humans ; Spine

PURPOSE: The present study developed formulas for estimation of standard liver volume (SLV) with high accuracy for the Korean population. MATERIALS AND METHODS: SLV estimation formulas were established using gender-balanced and gender-unbalanced measurements of anthropometric variables, body composition variables, and abdominal geometry of healthy Koreans (n=790). Total liver volume excluding blood volume, was measured based on CT volumetry. RESULTS: SLV estimation formulas as preferred in various conditions of data availability were suggested in the present study. The suggested SLV estimation formulas in the present study were found superior to existing formulas, with an increased accuracy of 4.0–217.5 mL for absolute error and 0.2–18.7% for percentage of absolute error. CONCLUSION: SLV estimation formulas using gender-balanced measurements showed better performance than those using gender-unbalanced measurements. Inclusion of body composition and abdominal geometry variables contributed to improved performance of SLV estimation.
Blood Volume ; Body Composition* ; Liver*

Bolld volume in 10 adult rabbits were measured by means of RISA dilution. The values were: eye plasma volume 0.0093 ml/gm eye, eye bolld volume 0.016 ml/gm eye and total blood volume of whole eye was 0.043 +/- 0.013(S.D.) ml. Total body plasma volume obtained simultaneously was 4.0% body weight and total body blood volume 6.9% body weight.
Adult ; Blood Volume* ; Body Weight ; Humans ; Plasma Volume ; Rabbits

BACKGROUND: Inoculated blood volume and residual antibiotic effects are the key variables that influence successful blood cultures especially in patients receiving antibiotics. In our hospital we have inoculated BacT/Alert blood culture bottles with 5 mL of blood, but recent reports about the BacT/Alert system recommend an inoculation of up to 10 mL of blood. METHODS: We studied about 931 paired aerobic blood culture sets and each set was inoculated with 3 mL (3 mL bottle) and 7 mL (7 mL bottle) of blood. The positive rates, the yield and speed of detecting microorganisms in the culture bottles were compared to the specimen information of antibiotic usage. We also analyzed the antibiotic effect on each set of blood cultures by inoculation with standard microorganisms (E. coli ATCC 25922 and S. aureus ATCC 25923) in the used bottle sets. RESULTS: Positive cultures were obtained from 98 sets (10.5%) and 161 organisms grew. Of 931 blood culture bottle sets, 744 (79.9%) were obtained from patients on antibiotic therapy. The positive rate of blood culture bottles from patients receiving antibiotic therapy was significantly lower than those from patients who were not receiving antibiotic therapy (P<0.05). Irrespective of antibiotic usage before using the blood cultures, the 7 mL bottle showed a better yield and speed of detecting microorganisms than the 3 mL bottles, but it was not statistically significant (P>0.05). When culture bottles with no growth (from patients receiving antibiotic therapy) were used, no differences were found in detection time and positive rates between 3 mL and 7 mL blood culture bottles that were artificially seeded with standard E. coli or S. aureus strains. CONCLUSIONS: Although antibiotic usage rate before using the blood cultures was high in our hospital, the 7 mL bottle showed a better yield and speed of detecting microorganisms than did the 3 mL bottle irrespective of antibiotic usage before using the blood cultures. This data suggests that blood volume elevation (from 5 mL up to 10 mL) can be recommended for BacT/Alert Blood culture systems.
Anti-Bacterial Agents ; Blood Volume* ; Humans

OBJECTIVETo assess the accuracy of cardiac output (CO) measured by transpulmonary thermodilution technique (TPTD)and explore the validity of intrathoracic blood volume index (ITBVI) for assessment of circulatory volume status.

METHODSTen immature pigs with a mean weight of (20.6±1.9)kg were studied during the conditions including normovolemia, hypervolemia, and hypovolemia. Simultaneous CO was measured in each condition using pulmonary artery thermodilution (PATD) method and TPTD. More specifically, CO (COPA) was determined with PATD, while CO (COTP) and ITBVI were determined with TPTD. All measurements were repeated 3 times. Central venous pressure (CVP) and heart rate were measured at the same time. The potential correlations of CVP and ITBVI with cardiac index (CI) and stroke volume index (SVI) in each blood volume status were analyzed.

RESULTSA total of 90 simultaneous measurements of COPA and COTP in 3 different blood volume conditions were made. The correlation coefficient between the two measurements was 0.977 (P<0.001) and the mean difference was (0.25±0.26)L/min (95%CI:0.20-0.30 L/min, P<0.001). The coefficient of variation of COTP was 3.7%, while COPA was 5.4%. Compared with those in normovolemia, CVP and ITBVI in hypervolemia significantly increased (P=0.002, 0.019), ITBVI in hypovolemia decreased significantly (P<0.001), and CVP in hypovolemia decreased insignificantly (P=0.05). Correlation analysis revealed a significant correlation between ITBVI with CI and SVI in normovolemia (r=0.741, P=0.014; r=0.885, P=0.001). In contrast, correlations between CVP with CI and SVI were poor.

CONCLUSIONSTPTD can accurately and precisely measure CO in different blood volume conditions. ITBVI measured by TPTD has better validity for the assessment of circulatory volume status than CVP.

No abstract available.
Blood Platelets ; Mean Platelet Volume ; Urticaria