1.Stable Expression of Coagulation Factors by RPS6 Promoter.
Wen-Hui ZHANG ; Wen-Tian WANG ; Ying CHI ; Hui-Yuan LI ; Feng XUE ; Ren-Chi YANG ; Lei ZHANG
Journal of Experimental Hematology 2023;31(2):489-494
OBJECTIVE:
To screen better promoters and provide more powerful tools for basic research and gene therapy of hemophilia.
METHODS:
Bioinformatics methods were used to analyze the promoters expressing housekeeping genes with high abundance, so as to select potential candidate promoters. The GFP reporter gene vector was constructed, and the packaging efficiency of the novel promoter was investigated with EF1 α promoter as control, and the transcription and activities of the reporter gene were investigated too. The activity of the candidate promoter was investigated by loading F9 gene.
RESULTS:
The most potential RPS6 promoter was obtained by screening. There was no difference in lentiviral packaging between EF1 α-LV and RPS6-LV, and their virus titer were consistent. In 293T cells, the transduction efficiency and mean fluorescence intensity of RPS6pro-LV and EF1 αpro-LV were proportional to the lentiviral dose. The transfection efficiency of both promoters in different types of cells was in the following order: 293T>HEL>MSC; Compared with EF1 αpro-LV, RPS6pro-LV could obtain a higher fluorescence intensity in MSC cells, and RPS6pro-LV was more stable in long-term cultured HEL cells infected with two lentiviruses respectively. The results of RT-qPCR, Western blot and FIX activity (FIX∶C) detection of K562 cell culture supernatant showed that FIX expression in the EF1 α-F9 and RPS6-F9 groups was higher than that in the unloaded control group, and there was no significant difference in FIX expression between the EF1 α-F9 and RPS6-F9 groups.
CONCLUSION
After screening and optimization, a promoter was obtained, which can be widely used for exogenous gene expression. The high stability and viability of the promoter were confirmed by long-term culture and active gene expression, which providing a powerful tool for basic research and clinical gene therapy of hemophilia.
Humans
;
Transduction, Genetic
;
Genetic Vectors
;
Hemophilia A/genetics*
;
Transfection
;
Blood Coagulation Factors/genetics*
;
Lentivirus/genetics*
2.Correlation between the Polymorphism of Coagulation-Related Genes and Lower Extremity Deep Venous Thrombosis.
Yao Ru JIANG ; Lei Lei NIU ; Na FENG ; Hao Liang FAN ; Qian Qian JIN ; Qiu Xiang DU ; Jie CAO ; Ying Yuan WANG ; Jun Hong SUN
Journal of Forensic Medicine 2021;37(2):145-150
Objective To investigate the correlation between the polymorphism of 4 coagulation-related genes, rs1799963 (coagulation factor V gene Leiden), rs6025 (prothrombin gene G20210A), rs1042579 (thrombomodulin protein gene c.1418C>T) and rs1801131 (methylenetetrahydroflate reductase gene) and lower extremity deep venous thrombosis (LEDVT). Methods The 4 genotypes mentioned above of 150 LEDVT patients and 153 healthy controls were detected by the kompetitive allele specific polymerase chain reaction (KASP), then related blood biochemical indicators were collected, binary Logistic regression was established to screen the independent risk factors of LEDVT, and the correlation between polymorphism of 4 coagulation-related genes and LEDVT and its indicators under different genetic modes after adjusting confounding factors were analyzed. Results Five variables, D-dimer, fibrinogen degradation product, homocysteine, sex and age might be the risk factors of LEDVT. These variables were put into 4 genetic inheritance models, and adjusted in binary Logistic regression. The results suggested that the mutations of rs1042579 were correlated with LEDVT under dominant inheritance mode. Conclusion The gene polymorphism of rs1799963, rs6025 and rs1801131 has no significant correlation with the formation of LEDVT. The gene polymorphism of rs1042579 plays a role under dominant inheritance mode, and might be an independent risk factor for formation of LEDVT.
Blood Coagulation/genetics*
;
Humans
;
Lower Extremity
;
Polymorphism, Genetic
;
Risk Factors
;
Venous Thrombosis/genetics*
3.Congenital factor X deficiency: a retrospective analysis of 11 cases.
Rong Wei LI ; Xiao Fan LIU ; Feng XUE ; Yun Fei CHEN ; Wei LIU ; Rong Feng FU ; Lei ZHANG ; Rong Chi YANG
Chinese Journal of Hematology 2022;43(1):26-30
Objective: To analyze the clinical characteristics, laboratory examination, diagnosis, treatment, and outcome of hereditary factor Ⅹ (FⅩ) deficiency. Methods: Clinical data of 11 patients with congenital FⅩ deficiency were retrospectively analyzed from July 2009 to February 2021. Results: There were 3 males and 8 females. Median age was 39 (5-55) years. The media duration of follow-up was 81.67 (1.87-142.73) months. Of the 11 patients, 10 had bleeding symptoms, 7 had ecchymosis or hemorrhage after skin bump, 7 had nosebleed, 6 had gingival hemorrhage, and 1 had muscle hematoma. Among the female patients, 6 had menorrhagia and 1 experienced bleeding after vaginal delivery. Family history of FⅩ deficiency was found in one case. Eight patients had a history of surgery, and four had postoperative bleeding. Laboratory findings were characterized by significantly prolonged activated partial thromboplastin time, prothrombin time, and decreased FⅩ activity (FⅩ∶C) . Four cases underwent gene mutation analysis and five new mutations were found. Four cases were treated with prothrombin complex concentrates (PCC) and seven cases with fresh frozen plasma (FFP) . One female patient had significantly reduced menstrual volume after PCC prophylactic therapy. One patient received FFP for prophylactic infusion with no bleeding during and after the operation. Conclusion: Most patients with congenital FⅩ deficiency had bleeding symptoms and there was no significant correlation between severity of bleeding symptoms and FⅩ∶C. Prophylaxis should be applied in patients with severe bleeding tendencies. Gene mutation test is significant for screening, diagnosis, and prognosis prediction of congenital FX deficiency.
Adolescent
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Adult
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Blood Coagulation Factors/therapeutic use*
;
Blood Coagulation Tests
;
Child
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Child, Preschool
;
Factor X Deficiency/genetics*
;
Female
;
Hemorrhage/drug therapy*
;
Humans
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Male
;
Middle Aged
;
Plasma
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Retrospective Studies
;
Young Adult
4.Development of inhibitor against hemophilia and prevention and management strategies forpatients with hemophilia.
Chinese Journal of Pediatrics 2013;51(8):631-634
Blood Coagulation Factor Inhibitors
;
antagonists & inhibitors
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blood
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Factor VIII
;
administration & dosage
;
antagonists & inhibitors
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immunology
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Hemophilia A
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drug therapy
;
genetics
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immunology
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Humans
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Immune Tolerance
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Isoantibodies
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blood
;
immunology
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Recombinant Proteins
;
adverse effects
;
immunology
;
therapeutic use
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Risk Factors
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Time Factors
5.Relationship between endothelial cell protein C receptor gene 6936A/G polymorphisms and deep venous thrombosis.
Xu-Dong CHEN ; Lu TIAN ; Ming LI ; Wei JIN ; Hong-Kun ZHANG ; Cheng-Fei ZHENG
Chinese Medical Journal 2011;124(1):72-75
BACKGROUNDDeep venous thrombosis (DVT) can result in pulmonary embolism, a fatal complication that is due to the dislodgement and movement of a blood clot (thrombus) from a limb into the lungs. Genetic risk factors related to DVT development include mutations in coagulation proteins, especially the endothelial protein C receptor (EPCR), a component of the anticoagulation protein C (PC) pathway. The objective of the present study was to analyze the relationship between the 6936A/G polymorphism in the EPCR gene and the occurrence of DVT.
METHODSThis study involved 65 patients with DVT and 71 age- and gender-matched healthy controls. Peripheral blood samples were collected from all subjects. Plasma levels of soluble EPCR (sEPCR) were measured by enzyme-linked immunosorbent assay. Genomic DNA was extracted and EPCR gene product was amplified by a standard PCR reaction. Gene product bands were sequenced to identify EPCR gene polymorphisms.
RESULTSIn the control group, the level of sEPCR in subjects with 6936AG genotype was significantly higher than that in subjects with 6936AA genotype ((0.97 ± 0.32) pg/ml vs. (0.61 ± 0.24) pg/ml, P < 0.01). Similarly in the DVT group, the level of sEPCR in subjects with the 6936AG were greater than that in subjects with the 6936AA genotype ((0.87 ± 0.21) pg/ml vs. (0.50 ± 0.18) pg/ml, P < 0.01). The sEPCR level in DVT patients was significantly higher than that in healthy controls ((0.68 ± 0.32) pg/ml vs. (0.54 ± 0.22) pg/ml, P < 0.05). The 6936AG genotype frequency in DVT patients was significantly higher than that in healthy controls (P < 0.05). In contrast, the 6936AA genotype frequency in DVT patients was lower than that in healthy controls (P < 0.05). Subjects carrying 6936AG had an increased risk of thrombosis (OR = 2.75, 95%CI: 1.04 - 7.30, P < 0.05).
CONCLUSIONSEPCR gene 6936A/G polymorphism is associated with increased plasma levels of sEPCR. Subjects carrying 6936AG likely have an increased risk of thrombosis.
Blood Coagulation Factors ; genetics ; Case-Control Studies ; Female ; Genetic Predisposition to Disease ; genetics ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Genetic ; genetics ; Receptors, Cell Surface ; genetics ; Venous Thrombosis ; genetics
6.Cellular microparticles and pathophysiology of traumatic brain injury.
Zilong ZHAO ; Yuan ZHOU ; Ye TIAN ; Min LI ; Jing-Fei DONG ; Jianning ZHANG
Protein & Cell 2017;8(11):801-810
Traumatic brain injury (TBI) is a leading cause of death and disability worldwide. The finding that cellular microparticles (MPs) generated by injured cells profoundly impact on pathological courses of TBI has paved the way for new diagnostic and therapeutic strategies. MPs are subcellular fragments or organelles that serve as carriers of lipids, adhesive receptors, cytokines, nucleic acids, and tissue-degrading enzymes that are unique to the parental cells. Their sub-micron sizes allow MPs to travel to areas that parental cells are unable to reach to exercise diverse biological functions. In this review, we summarize recent developments in identifying a casual role of MPs in the pathologies of TBI and suggest that MPs serve as a new class of therapeutic targets for the prevention and treatment of TBI and associated systemic complications.
Animals
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Astrocytes
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metabolism
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pathology
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Biological Transport
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Blood Coagulation Factors
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genetics
;
metabolism
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Brain
;
metabolism
;
pathology
;
physiopathology
;
Brain Injuries, Traumatic
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genetics
;
metabolism
;
pathology
;
physiopathology
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Cell-Derived Microparticles
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chemistry
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metabolism
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pathology
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Cytokines
;
blood
;
genetics
;
Disease Models, Animal
;
Disseminated Intravascular Coagulation
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genetics
;
metabolism
;
pathology
;
physiopathology
;
Gene Expression Regulation
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Humans
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Microglia
;
metabolism
;
pathology
;
Neurons
;
metabolism
;
pathology
;
Signal Transduction
7.Expression and characterization of fusion protein tTF/SA as a universal effector for targeting blood coagulation.
Zhen WANG ; Jiang-Hua YAN ; Jie-Ping WANG ; Lian-Ying XIE ; Na WU
Chinese Journal of Biotechnology 2007;23(2):218-222
UNLABELLEDTo prepare a novel fusion protein (tTF/SA) as a universal effector for targeting therapy of blood coagulation and to analyze its biological activities. The fusion gene tTF/SA was constructed by PCR, then inserted into expression vector pET22 b (+), and expressed in E. coli BL21 (DE3). The fusion protein was purified using Nickel-affinity chromatography column. The activities of tTF moiety of the fusion protein were analyzed by clotting assay and FX activation assay, and the binding activities of Streptavidin(SA) to Biotin(B) were analyzed using ELISA.
RESULTSThe recombinant plasmid tTF/SA/pET22 b (+) with the correct sequence was obtained. The fusion gene tTF/SA was expressed with high yield in E. coli BL21 (DE3). The purified fusion protein retain the abilities of activating FX, inducing blood coagulation, and binding Biotin. The fusion gene tTF/SA was successfully expressed in E. coli BL21 (DE3). The recombinant tTF/SA proteins retain the activities of TF and SA. The multitarget therapy of selectively inducing thrombosis in tumor blood vessels can be achieved by the combination of tTF/SA, as a universal effector, and biotinlated carriers directing to tumor blood vessels.
Animals ; Binding, Competitive ; drug effects ; Biotin ; metabolism ; Blood Coagulation ; drug effects ; physiology ; Chromatography, Affinity ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; genetics ; Gene Expression ; Mice ; Polymerase Chain Reaction ; Recombinant Fusion Proteins ; genetics ; metabolism ; pharmacology ; Streptavidin ; genetics ; metabolism ; pharmacology ; Thromboplastin ; genetics ; metabolism ; pharmacology ; Time Factors
8.Effects of realgar on tissue factor expression of NB4 and MR2 cells.
China Journal of Chinese Materia Medica 2003;28(6):553-556
OBJECTIVETo investigate the effects of Realgar on procoagulant activity (PCA), tissue factor expression and tissue factor mRNA transcription in acute promyelocytic leukemia (APL) cell lines NB4 and MR2 cells.
METHODNB4 and MR2 cells were treated with 300 micrograms.L-1 Realgar PCA of the treated cells was detected using one-stage clotting assay. TF antigen was detected by ELISA and TFmRNA by semi-quantitive RT-PCR.
RESULTThe PCA and TF antigen level in NB4 and MR2 cells were significantly higher than that in HL-60 and K562 cells. Realgar could down-regulate the membrane PCA, TF antigen and TF mRNA transcription of NB4 and MR2 cells in a time-dependent manner.
CONCLUSIONDown-regulating TF expression and PCA of NB4 and MR2 cells by Realgar may be one of the mechanism of its improvement effect on DIC-related hemorrhage of APL patients.
Antineoplastic Agents ; pharmacology ; Arsenicals ; pharmacology ; Blood Coagulation Factors ; Cysteine Endopeptidases ; metabolism ; Drug Resistance, Neoplasm ; Gene Expression Regulation, Leukemic ; HL-60 Cells ; Humans ; K562 Cells ; Leukemia, Promyelocytic, Acute ; metabolism ; pathology ; Materia Medica ; pharmacology ; Neoplasm Proteins ; metabolism ; RNA, Messenger ; genetics ; Sulfides ; pharmacology ; Thromboplastin ; biosynthesis ; genetics ; Tretinoin ; pharmacology
9.Clinical and laboratory features of patients with CD34(+) acute promyelocytic leukemia.
Jian-ying LIANG ; De-pei WU ; Yue-jun LIU ; Qin-fen MA ; Yong-quan XUE ; Ming-qing ZHU ; Zi-xing CHEN
Chinese Journal of Oncology 2009;31(3):196-198
OBJECTIVETo explore the expression of CD34 in patients with acute promyelocytic leukemia (APL) and investigate the clinical and laboratory features of CD34(+) APL patients.
METHODS262 APL patients diagnosed by chromosome analysis and/or fusion gene examination in the last five years were retrospectively analyzed in this study. To survey the expression of CD34 in those patients, all the cases were divided into two groups (CD34(+) APL vs. CD34(-) APL). The clinical features including age, gender, abnormal values of the peripheral hemogram before treatment, the complete remission (CR) rate and the incidence of DIC and laboratory data such as the results of morphology, immunology, cytogenetics and molecular biology (MICM) between those two groups were compared.
RESULTSOf the 262 APL patients, 38 (14.5%) cases were positive for CD34 expression. There were no statistically significant differences between CD34(+) APL and CD34(-) APL groups in gender and age (P > 0.05). Before treatment, the median level of WBC in CD34(+) APL was 25.92 x 10(9)/L, which was significantly higher than that of CD34(-) APL (5.3 x 10(9)/L, P < 0.05). CD34(+) APL by morphology classification were mostly of the subtypes M3b and M3v (65.8%), while these subtypes in CD34(-) APL (40.3%) were significantly less (P < 0.01). There were no statistically significant differences between the two groups compared in respect of complete remission (CR) rate and the incidence of DIC (P > 0.05). The expression level of CD34 in APL had correlation to the expression level of CD2, CD7 and CD117; the latter three phenotypes in CD34(+) APL were significantly higher than those in CD34(-) APL (P < 0.01). No significant difference was found between those two groups by chromosome analysis, but there was more PML-RAR-alpha transcript short form in CD34(+) APL than that in CD34(-) APL (P < 0.05).
CONCLUSIONCD34(+) acute promyelocytic leukemia is a unique subtype of APL with different biological characteristics.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antigens, CD34 ; blood ; Antigens, CD7 ; blood ; Antineoplastic Agents ; therapeutic use ; CD2 Antigens ; blood ; Child ; Disseminated Intravascular Coagulation ; etiology ; Female ; Humans ; Immunophenotyping ; Leukemia, Promyelocytic, Acute ; complications ; drug therapy ; genetics ; immunology ; Male ; Middle Aged ; Nuclear Proteins ; metabolism ; Phenotype ; Promyelocytic Leukemia Protein ; Proto-Oncogene Proteins c-kit ; blood ; Receptors, Retinoic Acid ; metabolism ; Remission Induction ; Retinoic Acid Receptor alpha ; Retrospective Studies ; Transcription Factors ; metabolism ; Translocation, Genetic ; Tretinoin ; therapeutic use ; Tumor Suppressor Proteins ; metabolism ; Young Adult