1.Research advances on burn blister fluid.
Hong Fei DONG ; Xi HUANG ; Shuang YOU ; Xian Hui LI
Chinese Journal of Burns 2022;38(10):994-998
Burns often cause the damaged tissue to produce a large amount of exudate and the formation of blisters on the wound. The burn blister fluid contains a large number of molecules related to wound healing, which can reflect the state of local tissue microenvironment of the burn wound. Analyzing relevant information such as cellular components, signal mediators, and protein molecules in burn blister fluid is helpful to understand the local reaction and tissue microenvironment of burn wounds, and then help clinical burn treatment. In this article, by understanding the production mechanism of burn blister fluid, discussing its role in wound evaluation, and integrating the research progress of burn blister fluid in proteomics, metabolomics, cellular components, and pharmacokinetics, we propose our thoughts and prospects on the research of burn blister fluid, in order to provide assistance for clinical evaluation and treatment of burn wounds, and also provide idea for the follow-up study of burn blister fluid.
Humans
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Blister/metabolism*
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Follow-Up Studies
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Burns/metabolism*
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Exudates and Transudates/metabolism*
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Wound Healing
2.Effect of burn blister fluid on human mesenchymal stem cell (MSC) in vitro and its phenotypic modulation in culture.
Li-Zhu LIU ; Xin-Zhou RONG ; Tao ZHANG ; Rong-Hua YANG ; Zhen WANG
Chinese Journal of Burns 2009;25(3):207-210
OBJECTIVETo study the effect of blister fluid obtained from burn patient on human MSCs in vitro and its phenotypic modulation in culture.
METHODSBlister fluid from burn patients was collected at 12, 24, 48 post burn hour (PBH). The human MSCs were isolated, cultured, amplified and identified in vitro, then were divided into A (culture with 20% blister fluid collected at 12 PBH) , B (culture with 20% blister fluid collected at 24 PBH), C (culture with 20% blister fluid collected at 48 PBH), N (with ordinary culture medium) groups. The growth of MSCs and micro-organisms in blister fluid were observed. Positive expression rates of CD44 and CK7 were detected by flow cytometry after culture for 8 days.
RESULTSBacterial and fungal growths were absent in 15 blister fluid samples. There was no obvious change in MSC morphology in each group. Compared with that of N group, the number of MSCs in A, B, C groups was decreased, especially in C group. CD44 positive expression rate in A, B, C groups was (83.0 +/- 3.1)%, (77.2 +/- 2.9)% and (65.1 +/- 2.3)%, respectively,which was obviously lower than that in N group [(89.5 +/- 3.2)%, P < 0.01]. CK7 positive expression rate in A, B, C groups was (24.06 +/- 0.11)%, (16.41 +/- 0.09)% and (4.48 +/- 0.07)%, respectively, which was obviously higher than that in N group [(3.87 +/- 0.04)%, P < 0.01].
CONCLUSIONSBurn blister fluid can obviously inhibit the growth of human MSC cultured in vitro, and may promote modulation of its phenotype to certain extent.
Blister ; metabolism ; Bone Marrow Cells ; cytology ; Burns ; Cell Differentiation ; Cell Separation ; Cells, Cultured ; Flow Cytometry ; Humans ; Mesenchymal Stromal Cells ; cytology
3.A Case of McArdle's Disease.
Sang Ik LEE ; Chang Won SONG ; Kyu Hyun PARK ; Sang Wook KIM
Journal of the Korean Neurological Association 1992;10(4):554-558
McArdle's disease is a disorder of carbohydrate metabolism, which is inhented as an autosomal recessive or occasionally an autosomal dominant trait. Hallmark of clinical features is exercise intolerence, I.e. muscle pain following strenuous exercise. Electrophysiologically insertion of an EMG needle shows that there is no electrical activity, differentiating this contracture from a muscle cramp. Histological examination of muscle biopsy specimen shows increase in glycogen and the presence of subsarcolemrnal blebs. We report a 23-year-old, male patient who presented clinical, electrophysiological, and histological findings compatible with McArdle's disease.
Biopsy
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Blister
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Carbohydrate Metabolism
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Contracture
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Glycogen
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Glycogen Storage Disease Type V*
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Humans
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Male
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Muscle Cramp
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Myalgia
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Needles
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Young Adult